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Effect of Gamiojeoksan Remnants used as Fertilizer on Growth and Yield of Korean mint
Jaihyunk Ryu,Seongkyu Choi,Kyeong Won Yun,Youngnam Seo,Kyoungsun Seo,Hyunjin Kim 한국자원식물학회 2009 한국자원식물학회지 Vol.22 No.3
In these experiments Gamiojeoksan remnant components were analyzed to recycle this medicinal herb remnant fertilizers. The basic growth of Korean mint by application of Gamiojeoksan remnants were higher than control. As the amount of fertilizers were increased, plant height, stem diameter, number of leaves and number of branches were increased. The growth and yield were the highest in the treatment of 30 g/pot. Weight of whole plant of Korean mint has a tendency to be heavy in application of herbs remnant than that of control.
Assessment of Growth and Seed Oil Composition of Kenaf (Hibiscus cannabinus L.) Germplasm
Jaihyunk Ryu,강시용,하보근,김동섭,김진백,Sang Hoon Kim 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.4
This study was conducted to evaluate the growth characteristics and fatty acid composition among 15 kenaf mutants derived fromthe kenaf germplasm C14 and 15 kenaf accessions originating from Russia, India, China, Iran, and Italy. The overall growth performance(plant height, stem diameter, flowering date, leaf, and flower size) of the stem color mutant lines derived from C14 are similarto those of the original variety. However, the flower color mutant lines derived from C14 showed flowering to occur 10 days laterwhen compared with the original variety and showed smaller leaf sizes than the original variety. Late-ripened kenaf accessions(Jinju, Auxu, and Jnagdae) can yield more bio-mass compared with early or medium-maturing germplasm. The late maturity kenaf(Auxu, Jinju, and Jangdae) has a higher oil percentage than the early maturity germplasm. Linoleic, oleic, and palmitic acids were thepredominant fatty acids in all kenaf seeds. The stem color mutant lines significantly surpassed the parental means of all saturatedfatty acids. In addition, the flower color mutant lines showed broad ranges of variation in oleic acid. The 15 accessions showed awide range of fatty acid compositions, spanning from 29.75 to 38.30% saturated fatty acids and 61.70 to 70.24% total unsaturatedfatty acids, and the late maturity kenaf has a higher linoleic acid percentage than the early maturity germplasm. The flowering periodwas highly positively (P ² 0.01) correlated with the plant height, stem diameter, oil percent, and linolenic acid (C18:3), and it was significantlynegatively (P ² 0.01) correlated with stearic acid (C18:0). These results will provide valuable information to assist theparental selection of kenaf breeding.
Chemical Components in the Leaves of Selected Mutant Cultivars of kenaf (Hibiscus cannabinus L.)
Jaihyunk Ryu,Sang-Wook Jeong,Seung Bin Im,Joon-Woo Ahn,Soon-Jae Kwon,Dong Sub Kim,Jin-Baek Kim,Sang Hoon Kim,Si-Yong Kang 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Kenaf (Hibiscus cannabinus L.) native to Africa can be used as fiber, food, feedstock and bio plastic. This study was carried out to evaluate the mineral, amino acid and vitamin contents of six selected kenaf cultivars which are enable to produce seed under Korean circumstance. The leaves of three mutant cultivars (Jangdae, Jeokbong and Baekma), two original cultivars (Jinju, C14) and one Chinese cultivar (Auxu) were harvested at flowering time. Mineral components of kenaf leaves, such as calcium, potassium, and mineral, did not showed significant differences among the cultivars. As major amino acids including proline and phenylalanine, significant differences were found in these kenaf cultivars. The Auxu cultivar contained the highest amount of essential amino acid (Phenylalanine, Leucine, Isoleucine, Valine, Methionine and Lysine). The amount of vitamin displayed significant differences such as vitamin E and vitamin K among these cultivars. Especially, Jangdae cultivar contained the highest amount of vitamin E and vitamin K. Thus, these data suggested that Jangdae and Auxu is the most desirable cultivar containing high amount of vitamin and amino acid.
Variation Analysis of in vitro Cultured Plant Rhizomes Based on RAPD
Jaihyunk Ryu,Chang-Hyu Bae 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04
RAPD (random amplified polymorphic DNA) analysis was examined to detect variation of in vitro cultured 30 rhizomes of Cymbidium goeringii and Cymbidium kanran, with long-term subculture, respectively. Out of 105 DNA bands detected, the 38 were polymorphic with a polymorphic rate 36.1% in the C. goeringii. Out of 126 DNA bands detected, the 58 were polymorphic with a polymorphic rate 46.0% in the C. kanran. The size of the amplified fragments of the two species ranged from about 200bp to 3,000bp. Genetic similarity matrix (GSM) shows from 0.810 to 1.00 with an average of 0.929 in the rhizomes of C. goeringii and 0.786 to 0.984 with an average of 0.915 in the C. kanran. The result indicate that the long-term in vitro cultured C. goeringii and C. kanran supplemented with growth regulators might be promoted in higher polymorphism and increased GSM. Thus, the higher polymorphism rate of in vitro cultured rhizomes might be resulted in long-term subculture and the plant growth regulators supplemented with the culture medium. The results provide as fundamental data to develop a new materials for plant breeding and resources plant.
( Jaihyunk Ryu ),( Soon-jae Kwon ),( Yeong Deuk Jo ),( Hong-il Choi ),( Kyung-yun Kang ),( Bo Mi Nam ),( Dong-gun Kim ),( Chang-hyun Jin ),( Jin-baek Kim ),( Ee-yup Kim ),( Seung Cheol Oh ),( Bo-keun 한국육종학회 2017 Plant Breeding and Biotechnology Vol.5 No.3
The Rubus fruit is an economically important berry crop that contains various functional compounds. The objective of this study was to analyze fruit qualities (i.e., pH, soluble solids content, titratable acidity, and mineral content) as well as fatty acid and phenolic compounds (i.e., ellagic acid and anthocyanins) among hybrid boysenberry lines developed by hybridization and gamma irradiation. There were no significant differences in the hybrid boysenberry fruit pH and titratable acidity (%) among the tested genotypes. However, the soluble solids content was higher in the BSA119 and BSA144 mutants than in the original genotype (BS_Hybrid). Meanwhile, linoleic acid was the most abundant fatty acid in the analyzed hybrid boysenberry fruits. The fatty acid composition did not differ significantly among the genotypes. The ellagic acid content of all genotypes ranged from 8.72 mg/100 g to 46.10 mg/100 g, with the highest concentration observed for the BSB127 genotype. Additionally, cyanidin-3-O-sophoroside (M-H<sup>+</sup>, 611 m/z) and cyanidin-3-O-glucoside (M-H<sup>+</sup>, 449 m/z) were the two major anthocyanins detected in the boysenberry and mutant genotypes, while cyanidin-3-O-glucoside was the predominant blackberry anthocyanin. The total anthocyanin concentrations of four mutant genotypes (i.e., BSA036, BSA078, BSA101, and BSB127) were significantly higher than that of the original genotype (382.0 mg/100 g). The highest total anthocyanin concentration was observed for the BSA078 genotype (467.9 mg/100 g). These results may be useful for identifying the optimal genotypes for breeding new cultivars with enhanced qualities and potential health benefits.
( Jaihyunk Ryu ),( Soon Jae Kwon ),( Yeong Deuk Jo ),( Chang Hyun Jin ),( Bo Mi Nam ),( Seung Young Lee ),( Sang Wook Jeong ),( Seung Bin Im ),( Seung Cheol Oh ),( Lan Cho ),( Bo Keun Ha ),( Si Yong K 한국육종학회 2016 Plant Breeding and Biotechnology Vol.4 No.2
Blackberries (Rubus fruticosus L.) are a good source of antioxidants and contain appreciable levels of functional compounds. This study was carried out to evaluate fruit qualities (pH, soluble solids content, titratable acidity, and minerals), functional compounds (total phenolic content, total flavonoid content, anthocyanins, and ellagic acid), and antioxidant activity among five mutated blackberry lines over three ripening stages to examine the effect of fruit maturation on functional compounds and antioxidant activity. The content of sugars, total phenolic content, total flavonoid content, and anthocyanins (cyanidin-3-O-glucoside, cyanidin-3-O-xyloside, cyanidin-3-O-malonylglucoside, and cyanidin-3-O-dioxalylglucoside) increased as fruit development advanced, whereas the ellagic acid content decreased as the fruit became more mature. Among the minerals tested, potassium showed the highest concentrations at all stages of development. The antioxidant activity was correlated with total phenolic content (P≤0.01), total flavonoid content (P≤0.01), and total anthocyanins (P≤0.05). The results indicate that Maple and B201 lines contained the highest anthocyanin content and exhibited greater antioxidant activity.
Effect of Electron Beam on in vitro Cultured Orchid (Cymbidium) Organs
Jaihyunk Ryu,Hyun-Su So,Hyun Suk Kang,Byung Cheol Lee,Chang-Hyu Bae 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04
Ionizing radiations have been effective mutagen sources to overcome the limitation of the useful genetic resources in natural environment. The study was conducted to investigate an effect of electron beam on organogenesis, growth patterns and genetic variation in the irradiated orchid organs (rhizome and protocorm). The in vitro cultured rhizomes of orchids (Cymbidium goeringii, C. kanran) were irradiated with the electron beam in the dose range of 15Gy to 2240Gy under the condition of various beam energy and beam current (10MeV/n and 0.5mA, 10MeV/n and 0.05mA, 2MeV/n and 0.5mA). Significant decreases in growth and organogenesis were observed by increase of intensity of electron beam irradiation. The irradiation intensity of lethal dose 50 (LD50) of the in vitro cultured orchid was estimated as approximately 500Gy to 1000Gy under 10MeV/n, and 1000Gy to 2000Gy under 2MeV/n. While, the optimal irradiation dose was approximately 100Gy for growth and organogenesis of the cultures under 10MeV/n with 0.05mA treatment, and 300Gy under 2MeV/n and 0.5mA electron beam condition. RAPD and ISSR analyses for the electron beam irradiated organs were performed to analyze genetic variation under the electron beam condition (2MeV/n and 0.5mA).
Jaihyunk Ryu,Bo-Keun Ha,Dong Sub Kim,Joon-Woo Ahn,Jin-Baek Kim,Sang Hoon Kim,Si-Yong Kang 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
This study was carried out to determine the effects of different harvesting stage on nutritive value and the quality of ensiled kenaf after fermentation among six kenaf cultivars. Six kenaf cultivars including two different maturity groups, mid-late maturing (Auxu, Jangdae and Jinju) and early-maturing (Baekma, Jeokbong and C14), were planted on May 14, 2013. Four harvesting times were made at intervals of 20 days from 15 July to 16 September, 2013. In all cultivars, the CP (crude protein) contents were decreased by a delayed harvest; the CP contents of kenaf silage were ranged from 92 to 184 g kg-1. Interestingly, there were no significant difference of NDF (neutral detergent fiber) and ADF (acid detergent fiber) content between the cultivars, however NDF and ADF content of kenaf silage were significantly increased by a late harvest. The silages of all cultivars displayed a low pH ranges less than 4.0, which is sufficient for stable storage. The lactic acid contents in Auxu were from 2.57 to 3.21%, which is higher than other cultivars. The harvesting stages did not affect to the concentrations of butyric acid and acetic acid in all cultivars. These results indicate that the harvesting stage is more important for the quality of kenaf silage than cultivar differences. And kenaf silage could be also used as fodder for ruminants.