1994년도 法醫剖檢 통계분석

서재관,김종열 大韓法醫學會 1995 대한법의학회지 Vol.19 No.2

This is a statistical observation by authors based on the data of so called unusual death brought to this institute for medicolegal autopsy by all of law enforcement agencies in Korea in the year of 1994. This report aims to reveal the facts on various causes of unusual death in Korea. The following are the summary of results, 1. The total numbers of autopsy made for the unusual deaths were 2,123 cases, of these 1,576 cases were males and 547 cases were females. 2. Violent deaths were 1,380 cases (65.0%), natural deaths were 657 cases (30.9%), and unknown cases were 86(4.1%). 3. For deaths due to injuries, blunt object injuries were the leading ahead. 4. Asphyxial deaths were 340 cases, 16.0 percents of the total and drowning were 139 cases showing the biggest number among the asphyxial deaths. 5. Deaths from abnormal temperature and electricity were death due to fire, burning, hypothermia, heatstroke and electrocution. 6. There were 5 cases for starvation 7. Infanticides were 8 cases, 0.4 percent of the total number. 8. For the death due to intoxication, 236 cases (11.1%) were recorded and the biggest number among these was CO intoxication. 9. For the natural death, 657 cases, 30.9 percents of the total were recorded and the death due to the disease of the cardiovascular system was taking the most of the total number with 323 cases. 10. Cause of death unknown due to negative autopsy and to the severe decomposition of the body were 86 cases, 4.1 percents of the total number

조직배양에서 얻어진 쪽파의 유전적 변이체들이 보여주는 단백질과 Isozyme의 유형분석

朴敬淑,李在悅,徐奉甫 慶北大學校 1992 論文集 Vol.52 No.-

Some genetic variants of Amphihaploid, Hypoamphidiploid, Amphidiploid are regenerated from tissue culture in Allium wakegi. They were applied to investigate the effects of the chromosomal differences on the gene expression of some isozymes. The electrophoretic migration patterns of total proteins were different at the position of MW5×10^5 Dalton among the genetic variants. Three different isozymes were characterized from the migration patterns of isozymes in the discontinuous polyacrylamide gel electrophoresis. Alcohol dehydrogenase(ADH) appeared 3 bands in the gel electrophoresis corresponding to 3 isozymes, that is allozymes distributed as single isozyme in each individual. Isocitric acid dehydrogenase(ICDH) appeared 5 bands in the gel and corresponds to 5 isozymes as allozymes which distributed single isozyme in each individual. Phosphoglucoisomerase(PGI) appeared 8 bands in electrophoresis and to 8 isozymes which originated from 2 genes and distributed more than two isozumes in each individual. According to the isozume expression in the genetic variants of Allium wagegi, the amplification of chromosomal numbers can affect neither to increase the isozyme numbers and concentration nor to express heterogeneous types in the genetic variants.

Trichloroethylen의 직업적 노출에 의한 독성간염 및 박탈성 피부염 1례

채홍재,이성관,이강진,김종율,이승철,신동현,문재동 大韓産業醫學會 2003 대한직업환경의학회지 Vol.15 No.1

28세 남자가 trichloroethylene을 이용한 세척 및 탈지작업에 종사한지 한 달만에 발열, 오한 및 홍반을 동반한 피부발진 등의 증상이 발현되었다. 황달을 동반한 간기능 부전의 소견을 보였으며 피부는 박탈되었다. 부신피질호르몬, 항히스타민제 및 간기능 보조제를 이용한 치료로 간기능 및 피부 병변은 호전되었다. 검사소견상 Ig E가 상승되었고 trichloroethylene에 의한 과민반응으로 추정되었다. Trichloroethylene을 사용하는 소규모 사업장에서의 노출억제 방안을 포함한 보건관리가 요구되며 특수건강진단 주기에 대한 변경이 필요하리라 사료된다. A 28-year-old male began working as a degreaser. The solvent used in the degreasing operation was trichloroethylene. Over the next month the man experienced fever, chills, and an erythematous skin rash and itching. At that time he had a marked elevation in his liver enzyme, with cholestasis. Over the next few days the rash persisted then peeled. There was an elevation of Ig E, and a positive patch test reaction to trichloroethylene. His dermatitis and hepatitis were considered to be mediated by a hypersensitivity mechanism.

건축공사시방서에서의 KS규격의 국제인증방안에 관한 연구

김경식,이재섭,김용수,이규진,조용훈,최완철,전재열,이현수,구재동,이기환 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

The purpose of this research is make out construction specifications for International Construction. Using off-shore products incur cost increase and longer delivery term of construction. and inconvenient on International Construction. Expand the range of additional local products conforming to performance. Life Cycle Cost. maintainability. operability. user's acceptability, etc.. And this study is suggest the standard formats and the techniques for construction specification.

Biochemical effect of Ginsenosides on chemical mediator release from Mast cell in Type I Hypersensitivity

Jai Youl Ro 대한천식 및 알레르기학회 ( 구 대한알레르기학회 ) 1993 추계학술대회 초록집 Vol.1993 No.-

The Inhibitory Mechanism of Aloe Glycoprotein (NY945) on the Mediator Release in the Guinea Pig Lung Mast Cell Activated with Antigen-Antibody Complexes

Jai Youl Ro,Byung Chul Lee,Myung Hee Chung,Seung Ki Lee,Chung Ki Sung,Kyung Hwan Kim,Young In Park 대한생리학회-대한약리학회 1998 The Korean Journal of Physiology & Pharmacology Vol.2 No.1

It has been reported that the g1ycoprotein extracted from A10e has strong anti-inflammatory response,However, there has been no research report yet about the effect of A10e on allergic hypersensitivity reactivity, By using guinea pig 1ung mast cells, this study aimed to examine the effects of A10e g1ycoprotein (NY945) on the mediator re1eases caused by mast cell activation, and a1so aimed to assess the effects of NY945 on the mechanism of mediator re1eases in the mast cell activation. We partially purified mast cell from guinea pig 1ung tissues by using the enzyme digestion, the rough and the discontinuous density percoll gradient method. Mast cells were sensitized with IgG1 (anti-OA) and challenged with ova1bumin. Histamine was assayed by fluorometric ana1yzer, 1eukotrienes by radioimmunoassay. The phospholipase D activity was assessed by the production of 1abe1ed phosphatidy1alcoho1. The amount of mass 1,2-diacy1g1ycero1 (DAG) was measured by the eH]DAG produced when pre1abe1ed with eH]myristic acid. The phosph01ipid methy1ation was assessed by measuring the incorporation of the eH]methy1 moiety into phosph01ipids of cellu1ar membranes. Pretreatment of NY945 (10 μg) significant1y decreased histamine and 1eukotrienes re1eases during mast cell activation. The decrease of histamine re1ease was stronger than that of 1eukotriene during mast cell activation. The phospholipase D activity increased by the mast cell activation was decreased by the dose-dependent manner in the pretreatment of NY945. The amount of DAG produced by PLC activity was decreased by NY945 pretreatment. The amount of mass 1, 2-diacy1g1ycero1 produced by activation of mast cells was decreased in the pretreatment of NY945. NY945 pretreatment strong1y inhibited the incorporation of the eH]methy1 moiety into phosph01ipids. The data suggest that NY945 purified from A10e inhibi엄 in part an increase of 1, 2-diacy1g1ycero1 which is produced by activating mast cells with antigen-antibody reactions, which is mediated via phosphatidylcholinephospholipase D and phosphatidylinosit01-phospholipase C systems, and then followed by the inhibition of histamine re1ease. Furthermore, NY945 reduces the production of phosphatidylch01ine by inhibiting the methy1transferase 1 and II, which decreases the conversion of phosphatidylcho1ine into arachidonic acid and inhibits the production of 1eukotrienes.

Effects of Ginsenosides on the Mechanism of Histamine Release in the Guinea Pig Lung Mast Cells Activated by Specific Antigen- Antibody Reactions

Jai Youl Ro,Young Soo Ahn,Kyung Hwan Kim 대한생리학회-대한약리학회 1997 The Korean Journal of Physiology & Pharmacology Vol.1 No.4

<P> We previously reported that some components of ginsenosides decreased mediator releases evoked by the activation of mast cells with specific antigen-antibody reactions. This study aimed to assess the effects of ginsenosides (Rb<SUB>2</SUB>, Re) on the mechanism of histamine release in the mast cell activation. We partially purified guinea pig lung mast cells by using enzyme digestion, the rough and the discontinuous percoll density gradient method. Mast cells were sensitized with IgG<SUB>1</SUB> and challenged with ovalbumin (OA). Histamine was assayed by fluorometric analyzer, leukotrienes by radioimmunoassay. Phospholipase D (PLD) activity was assessed more directly by the production of [<SUP>3</SUP>H]phosphatidylbutanol (PBut) which was produced by PLD-mediated transphosphatidylation in the presence of butanol. The amount of 1,2- diacylglycerol (DAG) were measured by the [<SUP>3</SUP>H]DAG labeled with [<SUP>3</SUP>H]palmitic acid or [<SUP>3</SUP>H]myristic acid. Pretreatment of Rb<SUB>2</SUB> (300 ㄍg) significantly decreased histamine release by 60%, but Re (300 ㄍg) increased histamine release by 34%. Leukotrienes release in Rb<SUB>2</SUB> was decreased by 40%, Re was not affected in the leukotrienes release during mast cell activations. An increasing PLD activity during mast cell activation was decreased by the dose-dependent manner in the pretreatment of Rb<SUB>2</SUB>, but Re pretreatment facilitated the increased PLD activity during mast cell activation. The amount of DAG produced by phospholipase C (PLC) activity was decreased by Rb<SUB>2</SUB> pretreatment, but Re pretreatment was not affected. The amount of mass DAG was decreased by Rb<SUB>2</SUB> and Re pretreatment during mast cell activation. The data suggest that Rb<SUB>2</SUB> purified from Korean Red Ginseng Radix inhibits the DAG which is produced by the activation of mast cells with antigen-antibody reactions via both phosphatidylinositide-PLC and phosphatidylcholine- PLD systems, and then followed by the inhibition of histamine release. However, Re increases histamine release by stimulation of DAG production, which is mediated by phosphatidylcholine-PLD system rather than by phosphatidylinositide-PLC system, but inhibits the mass DAG production. Thus, it could be inferred that other mechanisms play a role in the increase of histamine release during mast cell activation.

Cromakalim Blocks Membrane Phosphoinositide Activated Signals in the Guinea Pig Lung Mast Cells Stimulated with Antigen-Antibody Reactions

Jai Youl Ro,Ji Young Kim,Kyung Hwan Kim 대한생리학회-대한약리학회 1998 The Korean Journal of Physiology & Pharmacology Vol.2 No.2

<P> Cromakalim (BRL 34915), known as an airway smooth muscle relaxant, inhibited the releases of mediators in the antigen-induced mast cell activation. It has been suggested that cromakalim, in part, inhibited mediator releases by inhibiting the initial increase of 1,2-diacylglycerol (DAG) produced by the activation of the other phospholipase system which is different from phosphatidylcholine-phospholipase D pathway. The aim of this study is to further examine the inhibitory mechanism of cromakalim on the mediator release in the mast cell activation. Guinea pig lung mast cells were purified by using enzyme digestion and percoll density gradient. In purified mast cells prelabeled with [<SUP>3</SUP>H]PIP<SUB>2</SUB>, phospholipase C (PLC) activity was assessed by the production of [<SUP>3</SUP>H]insitol phosphates. Protein kinase C (PKC) activity was assessed by measuring the protein phosphorylated from mast cells prelabeled with [γ-<SUP>32</SUP>P]ATP, and Phospholipase A<SUB>2 </SUB>(PLA<SUB>2</SUB>) activity by measuring the lyso-phosphatidylcholine produced from mast cell prelabeled with 1-palmitoyl-2-arachidonyl phosphatidyl-[<SUP>14</SUP>C]choline. Histamine was assayed by fluorometric analyzer, and leukotrienes by radioimmunoassay. The PLC activity was increased by activation of the passively sensitized mast cells. This increased PLC activity was decreased by cromakalim pretreatment. The PKC activity increased by the activation of the passively sensitized mast cells was decreased by calphostin C, staurosporine and cromakalim, respectively. The PLA<SUB>2</SUB> activity was increased in the activated mast cells. The pretreatment of cromakalim did not significantly decrease PLA<SUB>2</SUB> activity. These data show that cromakalim inhibits histamine release by continuously inhibiting signal transduction processes which is mediated via PLC pathway during mast cell activation, but that cromakalim does not affect PLA<SUB>2</SUB> activity related to leukotriene release.

Effects of a Mixture of Three Extracts of Wolfberry, Chives and Graviola on the Erectile Dysfunction Induced by Bilateral Cavernous Nerve Injury in Rats

Jai Youl Ro,Gwan Ui Hong,신연호,Chung Myung-Hee 건강기능식품미래포럼 2022 건강기능식품미래포럼 학술지 Vol.2 No.4

Although some chemicals are available for erectile dysfunction (ED), efforts have been also made to seek health functional foods that can improve ED. Lycium barbarum (wolfberry) and Allium tuberosum (chives) were reported to enhance erectile function. And Annona muricata L is known to have anti-inflammatory and antioxidant actions. We assumed that if the three plants were used together, they may enhance erectile function synergistically. Hyunsung Vital Co. Ltd. prepared a mixture of water extracts of the three plants and named it Jikdaijangryeuk (JDJR). In the present study, JDJR was tested for the effect of improving ED in the rats subjected to bilateral corpus cavernous nerve injury (BCNI). BCNI decreased intra-cavernous pressure and also induced biological responses in the penile tissue that lead to ED, which were decreases in the expressions of endothelial nitric oxide (NO) synthase (eNOS) and nervous NO synthase (nNOS) that produce NO (a stimulator of 3′,5′-cyclic guanosine monophosphate [cGMP] synthesis), neurofilament-1 (a marker of nerve fibers) and an anti-apoptotic protein (Bcl2) and in the amounts of NO, cGMP (a blood vessel dilator) and smooth muscle as well as increases in the expression of inducible NO synthase (iNOS) (an inducer of microvasculature dysfunction), phosphodiesterase-5 (a cGMP destroyer), apoptotic molecules (caspase-3 and Bax) and transforming growth factor-β (a fibrosis inducer) and in the amount of asymmetric dimethylarginine (an endogenous NO synthases inhibitor). These data indicate that BCNI suppresses function of NO/cGMP axis and causes apoptosis and fibrosis of cavernous tissue. JDJR, however, reversed all these responses in a dose-dependent manner and the effects of JDJR were comparable to those of sildenafil (a positive control). The powder of Platycodon grandiflorum (a negative control) did not show the effects. These data support that JDJR has the action to enhance erectile function and thus, may be of help for ED.

Gα12 and Gα13 Subunits Modulate Ca(2+) - Induced Histamine Release in Human Umbilical Cord Blood - Derived Mast Cells

(Jai Youl Ro),(Ji Young Kim),(Ji Hee Ha),(Chang Ho Lee) 한국미생물생명공학회 2002 Journal of microbiology and biotechnology Vol.12 No.3

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