複合 韓藥劑 淸籬滋坎湯의 抗癌效果 및 作用機轉 糾明

Cho, Kyung-Sam,Kim, Si-Young,Park, Jai-Kyung,Choi, Seung-Hoon,Chung, Se-Young,Yoon, Hwi-Joong 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung Sam Cho¹Si Young Kim¹, Jai Kyung Park²,Seung Hoon Choi³,Se Young Chung⁴, Hwi Joong Yoon¹¹College of Medicine, ²East-West Medical Research Institute, ³College of Oriental Medicine, ⁴College of Pharmacology, Kyung Hee University,Seoul, Korea. The Anti-cancer Effect of Oriental Medicine Chung-Ri-Ja-Gam-Tang in the Mouse with Metastatic Lung Cancer. Proceedings of International Symposium on East-West Medicine, Seoul. 231-243, 1999.-The oriental medicine Chung-Ri-Ja-Gam-Tang is an herbal medicine which has been used for pulmonary diseases and known as having immune stimulatory effects. It has been known effective in lung cancer. So we studied the effects and the mechanisms of this herbal medicine it the mouse with metastatic lung cancer. The metastatic lung cancer of the mouse was produced with melnoma cell line(B16BL/6).5×10□/mouse tumor cells were injected intravenously to the CDF1 mouse via tail vein. The mice were divided 4 groups. The first group was treated with 50mg/kg extract of Chung-Ri-Ja-Gam-Tang per oral for 10 days after cancer cell injection, second group treated with saline after cancer cell injection, the third group with medicine without cancer cell, and the last group with saline only. After 10 days treatments some of the mice were scarificed and the lung and spleen was removed. The survival duration, weight change, the number of metastatic cancer nodule of the lung, the NK cell activity, the capacity of cytokines(INF-γ,INF-α,IL-2) production and the proliferation activities of mouse lymphocytes were measured. The surival times of the group 1 mice were longer and the weight loss was less than the group 2 significantly. The number of the metastatic nodule of the lung were decresed in the group 1 than the group 2. The production of INF-γ,was increase in group 2 than group 3 and 4, IL-2 production was increased in group 1 than group 2,3,4 significantly. There was no difference in TNF- α production and proliferation activity of lymphocyte in each group. The NK cell activity was significantly increased in group 1 than group 2,3,4. We conclude that the Chung-Ri-Ja-Gam-Tang has the effect of increasing the NK cell activity of the CDF1 mouse with metastatic lung cancer(B16BL/6). And it is suggested that the increased production of the IL-2 is the mechanisms of enhanced NK cell activity.

조직배양에서 얻어진 쪽파의 유전적 변이체들이 보여주는 단백질과 Isozyme의 유형분석

朴敬淑,李在悅,徐奉甫 慶北大學校 1992 論文集 Vol.52 No.-

Some genetic variants of Amphihaploid, Hypoamphidiploid, Amphidiploid are regenerated from tissue culture in Allium wakegi. They were applied to investigate the effects of the chromosomal differences on the gene expression of some isozymes. The electrophoretic migration patterns of total proteins were different at the position of MW5×10^5 Dalton among the genetic variants. Three different isozymes were characterized from the migration patterns of isozymes in the discontinuous polyacrylamide gel electrophoresis. Alcohol dehydrogenase(ADH) appeared 3 bands in the gel electrophoresis corresponding to 3 isozymes, that is allozymes distributed as single isozyme in each individual. Isocitric acid dehydrogenase(ICDH) appeared 5 bands in the gel and corresponds to 5 isozymes as allozymes which distributed single isozyme in each individual. Phosphoglucoisomerase(PGI) appeared 8 bands in electrophoresis and to 8 isozymes which originated from 2 genes and distributed more than two isozumes in each individual. According to the isozume expression in the genetic variants of Allium wagegi, the amplification of chromosomal numbers can affect neither to increase the isozyme numbers and concentration nor to express heterogeneous types in the genetic variants.

대학생의 신체발육 및 건강상태에 관한 조사연구 -1980년도 충북대학교 신입생을 중심으로-

이경순, 노재섭, 박재영 忠北大學校 學生生活硏究所 1980 學生生活硏究 Vol.5 No.1

본문참고

CT 유도하의 경피적 폐생검 : 임상적 의의 및 합병증에 관한 연구 A Study of Clinical Significance and Complication

박재성,백상현,차장규,홍현숙,김대호,박성진,조준희,임한혁,이혜경 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.2

Purpose : To determine the diagnostic accuracy and predicting factor of pneumothorax on CT-guided percutaneous transthoracic needle biopsy. Materials and Methods : Using automated biopsy devices, 107 CT-guided percutaneous needle biopsies of pulmonary lesions were performed. Final diagnosis was made with operation or other methods. We retrospectively analyzed the frequency of complications and diagnostic yields of 107 biopsies. The clinical features, treatment, and courses of complications were reviewed, and risk factors of pneumothorax such as depth and size of lesion, CT emphysema score of whole lung and pathway of needle, and the result of pulmonary function test were analyzed. Result : 96 biopsies(89.7%) yielded sufficient tissue for pathologic evaluation. For cases of malignant and benign disease, sensitivity was 91.5% and 87.5% respectively; the corresponding figures for diagnostic accuracy were 88.1% and 77.1%. So, sensitivity and diagnostic accuracy of percutaneous transthoracic needle biopsy was 89.7% and 83.2%. Eighteen(16.8%) of 107 patients developed pneumothorax and 3 of these required application of chest tube or percutaneous catheter drainage. In the group with pneumonthorax, the lesion was significantly smaller(p=0.029) and higher CT emphysema score of pathway of needle(p=0.032). But there were no significant difference between two groups(with or without pneumothorax) for depth of lesion, pulmonary function test(% predictive value of FEV1 and FEV1/FVC) and CT emphysema score of whole lung. Conclusion : CT-guided transthoracic needle biopsy of the pulmonary lesions is safe and easy method of obtaining specimens of good quality and quantity. It may be an useful procedure in the diagnosis of localized pulmonary lesions. For the prediction of pneumothorax, size of lesion and CT emphysema score of pathway of a needle are significant risk factors.

제조합균주 E. coli Ck1092가 생산하는 2,3-Dihydroxybiphenyl Dioxygenase의 정제 및 특성

박효남,김영수,김영창,김치경,임재윤 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

4-CB 분해균주인 Pseudomonas sp. P20으로부터 pcbC 유전자를 클로닝하여 얻은 E. coli CK1092로부터 2,3-DHBP dioxygenase을 분리, 정제하여 효소적 특성을 조사하였다. 효소의 정제는 acetone 침전, DEAE-Se-phadex A-25 ion exchange chromatography, preparative electrophoresis 방법으로 정제하였다. 2,3-DHBP dioxygenase의 분자량은 약 270 kDa으로 추정되며, SDS-PAGE에 의한 분자량은 34 kDa이였다. 따라서, 동일한 subunit 8개 존재하는 octamer로 추정된다. 이 효소는 2,3-DHBP에 대해 높은 기질특이성을 보였으며, 3-methylcatechol, 4-methylcatechol, 4-chlorocatechol에 대해서는 활성을 보이지 않았다. 2,3-DHBP에 대한 Km 값은 18 μM이였으며 30μM 이상의 기질농도에서 활성이 감소하였다. 효소활성의 최적 pH는 8.0이였으며, pH 7.0~10.0 범위에서는 안정하였고, 최적 활성 온도는 40~60℃이며, 60℃까지는 비교적 안정하였다. 또한, 이 효소는 Cu^2+, Fe^2+, Fe^3+ 이온들에 의하여 효소활성이 저해되었고, H_2O_2와 EDTA에 의해서도 활성이 저해되었으며, 10%의 유기용매에 의해서 안정화되지 않았다. 효소활성부위를 알아보기 위해 화학변형제를 처리해 본 결과 tryosine, tryptophan과 histidine이 효소활성에 관여할 것으로 추정된다. 2,3-DHBP dioxygenase was purified from E. coli CK1092 carrying the pcbC gene, which was cloned from 4-chlorobiphenyl-degrading Pseudomonas sp. P20. Purification of this enzyme was done by acetone precipitation, DEAE-Sephadex A-25 ion exchange chromatography, and preparative gel electrophoresis. The molecular weight of subunit was 34 kDa determined by SDS-PAGE, and that of native enzyme was about 270 kDa. It suggests that this enzyme consist of eight identical subunits. This enzyme was specifically active against only 2,3-DHBP as a substrate with 18 μM of Km value, but not catechol, 3-methylcatechol, 4-methylcatechol and 4-chlorocatechol. The optimal pH and temperature of 2,3-DHBP dioxygenase were pH 8.0 and 40~60℃. The enzyme was inhibited by Cu^2+, Fe^2+ and Fe^3+ ions, and was inactivated by H_2O_2 and EDTA. The lower concentrations of some organic solvents such as acetone and ethanol don't stabilize the activity of 2,3-DHBP dioxygenase. The enzyme was completely inactivated by adding the reagents such as N-bromosuccinimide, iodine and p-diazobenzene sulfonic acid.

국민학교 아동의 체력실태 : 형태와 운동능력 중심으로 Based on Physique and Motor Fitness

朴庄平,李光宰,盧成圭,洪寬伊,李京濟 江原大學校附設體力硏究所 1980 江原大學校附設體育科學硏究所論文集 Vol.- No.5

신동맥 협착 정도에 따른 도플러 초음파 소견

박재성,이혜경,김대호,최득린,권귀향,홍현숙,임한혁,김기정 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

Renal artery stenosis is the one of causes of hypertension in a small percentage of all patients but is only anatomical cause that can be treated by means of surgery and percutanecous transluminal angioplasty. Angiography is the golden standard for diagnosis of renal artery stenosis, but a little invasive. With the advent of abdominal Doppler ultrasonography, renal artery stenosis could be diagnoed noninvasively. The experienced hands appears to be valuable in the doppler examination, ut some investigators consider it almost useless. The authors studied the effect of stenosis of renal artery on the downstream intrarenal blood flow, a snare was placed around renal artery in 12 rabbits and compressed to create stenosis while Doppler ultraonographic curve and flow index were obtained from main renal and interlobar arteries by means of placement of 10-MHz curved linear tranducer directly on the surface of kidney. The Doppler measurements were compared with the percentage of stenosis on angiograms. The results were as follow: 1. Ten satisfactory angiograms and Doppler ultrasonograms were obtained. 2. Three renal arteries were created stenosis below 70% (Group A), Seven were above 70% (Group B). 3. The ranges of RI (resistance index) were 0.61-0.86 (mean 0.78±0.154) at main renal arteries, 0.57-0.85 (mean 0.77±0.231) at interlobar artery. The range of PS (peak systolic velocity) were 13.1-28.8 (mean 21.65±4.445) m/sex at main renal artery, 5.2-18.1 (mean 11.74±4.095) m/sec at interlobar arteries. The RI of rabbits were higher than those of human (mean 0.58±0.05). 4. The range of RI in stenotic arteries of group B were 0.45-0.74 (mean 0.57±0.143) at main renal artery, 0.52-0.65 (mean 59±0.051) at interlobar arteries. The range of PS were 11.2-21.3 (mean 15.04±3.755) m/sec at main renal arte교, 5.7-11.3 (mean 8.24±2.056) at interlobar artery. The difference were statistical y significant (P=0.0302), but without remarkable change in group A. 5. Doppler ultrasonographic curve during progressive compression of renal artery showed dampened systolic waveform and diagonal upstroke at main renal arteries (71.4%) and interlobar arteries (85.7%) in group B, but without remarkable change in group A. 6. Release of the arterial compression caused higher than normal peak systolic velocity initially, at main renal arteries (42.9%) and interlobar arteries (28.6%). but the curve of all rabbits returned to normal within 5 minutes. The Doppler ultrasonographic index and curve were not changed on follow up Doppler ultrasonogram at 5 min. 10 min. 30 min. and 1 hour. The change of the systolic curves that compressed experimentally is identical to change in the pulsus tardus and parvus waveforms seen in both acute and chronic severe renal artery stenosis in humans, can helpul for the diagnosis of renal artery stenosis and the effects of treatment by percutaneous tranluminal angioplasty and surgery.

경부 림프절 비대의 진단에 있어서 초음파 유도하의 핵 생검의 유용성

김은경,곽진영,유재경,박정수,정웅윤,양우익,오기근 대한초음파의학회 2003 ULTRASONOGRAPHY Vol.22 No.4

가정에서 제조된 재래식 된장, 간장, 고추장 중에 존재하는 Ochratoxin A의 효소면역분석법에 의한 잔존량 분석

김철재,박경란,송태희 숙명여자대학교 환경과학연구소 1995 환경과학 Vol.2 No.-

본 연구에서는 재래 방법에 따라 생산되고 있는 전통발효식품(된장, 간장, 고추장)에 함유되어 있는 Ochratoxin A(OT-A)를 면역학적 정량분석법인 Enzyme-Linked Immuno-sorbent Assay(ELISA) 법을 개발하여 분석하였다. 각 가정에서 생산하여 소비되는 장류 즉, 된장 13종, 간장 12종, 고추장 14종을 전국적으로 수거하여 분석을 실시하였다. OT-A를 정량 조사한 표준곡선의 작성 결과 ELISA 방법의 sensitivity는 20pg/assay 이었으며, 본 실험에서 사용한 면역분석법의 OT-A 회수율은 90%이상이었다. OT-A의 잔존량은 가정에서 생산하여 소비되는 된장이 7.1±3.7ng/g, 간장이 2.1±2.6ng/g, 그리고 고추장이 4.0±1.9ng/g이었으며, OT-A에 대한 오염도가 비교적 낮은 것으로 나타났다. 한편 OT-A의 가열안정성 시험에서는 60, 90, 120℃에서 120분까지 가열 처리하여 OT-A의 잔존량을 조사하였던 바 121℃의 고온에서 120분까지도 안정하였다. This study was undertaken to develop the analytical procedures, Enzyme-Linked Immunosorbent Assay(ELISA) for the quantitative detection of ochratoxin A(OT-A) in the home-made 13 Doenjang, 12 Kanjang, and 14 Gochujang collected throughout the country. The detection limit in ELISA was leveled as low as 20 pg/assay, and that the OT-A recorvery rates from sample clean-up were appeared to be more than 90%. Therefore, ELISA was the appropriate tool to detect the residual OT-A in the traditional fermented foods. The residual contents of OT-A in the home-made traditional fermented foods were 7.1 《3.7 ng/g for Deonjang, 2.1《2.6ng/g for Kanjang, and 4.0《1.9ng/g for Gochujang. Comparatively it was low,and OT-A seemed to be carried over Doenjang to Kanjang from Meju. At heating stability test of OT-A in the traditional fermented foods was it found to be stable even at 121℃ for 120 min.

유기인제 중독 후 발생한 중간형 마비 증후군

고재욱,박준석,이경룡,정성필,이한식 대한응급의학회 2000 대한응급의학회지 Vol.11 No.4

Background: Intermediate myasthenia syndrome(IMS) is thought to have clinical importance because it may cause sudden respiratory failure during the recovery phase of a cholinergic crisis of organophosphate poisoning. We designed this study to identify the prevalence, the inducing agent, clinical predictor, and the proposed treatment of IMS. Methods: Patients who had admitted with the diagnosis of acute organophosphate poisoning from 1992 to 1998 at two teaching hospitals were enrolled in this study. We selected the cases of IMS based on a review of medical records using modified He' s criteria. Results: Twelve(12) out of 110 patients with acute organophosphate poisoning were diagnosed for a prevalence at 10.9%. The drug inducing IMS were identified as dichlorvos, fenthion, EPN, methidathion, and phosphamidon. The occurrence of IMS was not related to either the initial treatment with atropine and pralidoxime, or the level of serum cholinesterase. Complications were pneumonia, sepsis, pancreatitis, and pseudomembranous colitis, etc. Eleven(11) patients were discharged without sequelae, and one patient was discharged as a hopeless case. Conclusion: This study suggests that IMS is not rare, so close observation is required to detect IMS in organophosphate-poisoning patients. Also, more studies are required to find predictors and treatments.