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Jin, Jae-Youll,Park, Jin-Soon,Lee, Jong-Kuk,Park, Kwang-Soon,Lee, Dong-Young,Yum, Ki-Dai Korea Institute of Ocean Science Technology 1999 Ocean and Polar Research Vol.21 No.2
To meet increasing needs for environmentally sustainable management of coastal area, there has been compelling pressure to establish a cost-effective and long-term coastal water quality (CWQ) monitoring system. A remote CWQ monitoring system, STAMP, has been developed and is in operation along the route between Kyema harbor and Anma Island in the southwestern coastal area of Korea. STAMP uses a PCS phone as a telemetry unit to transmit acquired data for monitoring general water quality parameters, and a routinely operating coastal passenger ship or car ferry. STAMP has various merits of low-cost operations; long-term monitoring with secure instrumentation; and stable real-time telemetry of acquired data with-out the loss and noise. It is expected that the system will serve as a very useful tool in the CWQ managing programs of Korea taking the advantage of many coastal passenger ships in various routes including the ships departing from the coastal industrial cities. The acquired data compiled on suspended surface sediment concentrations (SSSC) will be also valuably helpful in understanding the sediment budget across the routes of the vessel.
Behavior of Currents and Suspended Sediments around a Silt Screen
Jin, Jae-Youll,Chae, Jang-Won,Song, Won-Oh,Park, Jin-Soon,Kim, Sung-Eun,Jeong, Weon-Mu,Yum, Ki-Dai,Oh, Jae-Kyung Korea Institute of Ocean ScienceTechnology 2003 Ocean and Polar Research Vol.25 No.suppl3
The behavior of Suspended Sediment Concentrations (SSC) around a silt screen in a microtidal coastal area was hydrodynamically measured. The current speed at the mid-layer about 30m downstream of the screen reduces to about half that at the same distance upstream. It was caused by the contraction of the vertical section due to the screen. Even during a relatively weak storm period the SSC increases to that of the value caused by dredging. Section-averaged SSC at the downstream of the screen is higher by about 60% than that at the upstream, suggesting that the silt screen plays an adverse effect rather than a constructive role in the reduction of SSC generated by dredging.
Jin, Jae-Youll,Hwang, Keun-Choon,Park, Jin-Soon,Yum, Ki-Dai,Oh, Jae-Kyung The Korean Society of Oceanography 1999 Journal of the Korean Society of Oceanography Vol.34 No.4
Seawater sampling is the primary task for the study of the marine environmental parameters that require shipboard or laboratory experiments for their analyses, and is also required for the calibration of some instruments for in situ measurement. A new automatic bottle (AUTTLE) is developed for seawater sampling at any desired time and water depth by self-triggering. Both any type of single or assembled mooring for 15 days and manual actuation by using a remote messenger as existing instantaneous single point water samplers are possible. Its sampling capacity and the resolution of time setting are 2 liters and 1 second, respectively. The result of a field experiment with an optical backscattering sensor (OBS) and a total of 14 AUTTLES for the in situ calibration of the OBS shows that the AUTTLE must improve our understanding on the behavior of the sand/mud mixtures in the environments with high waves and strong tides. The AUTTLE will serve as a valuable instrument in the various fields of oceanography, especially where synchronized seawater sampling at several sites is required and/or the information in storm period is important.
New and Improved Time-selective Self-triggering Water Sampler: AUTTLE
Jin, Jae-Youll,Hwang, Kuen-Choon,Park, Jin-Soon,Eo, Young-Sang,Kim, Seong-Eun,Yum, Ki-Dai,Oh, Jae-Kyung Korea Institute of Ocean Science Technology 2000 Ocean and Polar Research Vol.22 No.2
Time-selective self-triggering water sampler, AUTTLE developed by Jin et al. (1999) has been improved in order to prevent pre-deposition of suspended sediments (SS) before sampling. By using two solenoids, the improved sampler is able to be moored or deployed with inclination. Its position is changed to horizontal position by activating the first solenoid, and then the endcaps of the sampling bottle are closed by the second solenoid that is driven three times to minimize possible failure of sampling. An external control unit for setting sampling time has been also constructed. Additionally, the electric circuit housing of the sampler has been modified to be detached from the sampling bottle when operating manually. Its performance has been confirmed through flume tests and a field experiment. It will serve as a valuable tool in the various fields of oceanography and environmental engineering, especially where seawater sampling synchronized at several sites and/or the information in storm period is important.
Lee, Kwang-Youll,Heo, Kwang-Ryool,Choi, Ki-Hyuck,Kong, Hyun-Gi,Nam, Jae-Sung,Yi, Young-Byung,Park, Seung-Hwan,Lee, Seon-Woo,Moon, Byung-Ju The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.4
A biocontrol bacterium Bacillus licheniformis N1 grown in nutrient broth showed no chitinolytic activity, while its genome contains a gene which encodes a chitinase. The gene for chitinase from B. licheniformis N1 was amplified by PCR and the deduced amino acid sequence analysis revealed that the chitinase exhibited over 95% identity with chitinases from other B. licheniformis strains. Escherichia coli cells carrying the recombinant plasmid displayed chitinase activity as revealed by the formation of a clear zone on chitin containing media, indicating that the gene could be expressed in E. coli cells. Chitinase gene expression in B. licheniformis N1 was not detected by RT-PCR analysis. The protein was over-expressed in E. coli BL21 (DE3) as a glutathione S-transferase fusion protein. The protein could also be produced in B. subtilis 168 strain carrying the chitinase gene of N1 strain. The crude protein extract from E. coli BL21 carrying GST fusion protein or culture supernatant of B. subtilis carrying the chitinase gene exhibited enzyme activity by hydrolyzing chitin analogs, 4-methylumbelliferyl-$\beta$-D-N,N'-diacetylchitobioside and 4-methylumbelliferyl-$\beta$-D-N,N',N"-triacetylchitotrioside. These results indicated that even though the chitinase gene is not expressed in the N1 strain, the coding region is functional and encodes an active chitinase enzyme. Furthermore, B. subtilis 168 transformants expressing the chitinase gene exhibited antifungal activity against Fulvia fulva by suppressing spore germination. Our results suggest that the proper engineering of the expression of the indigenous chitinase gene, which will lead to its expression in the biocontrol strain B. licheniformis N1, may further enhance its biocontrol activity.