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재조합 균주 Bacillus subtilis가 생산하는 Strept-omyces albus KSM-35 Amylase의 정제 및 특성
이재우, 위성언, 윤옥현 김천대학교 1997 김천대학교 논문집 Vol.18 No.-
Amylase encoded by the samy gene of Streptomyces albus KSM-35 was produced from Bacillus subtilis LKS88 carrying a recombinant plasmid PASA240. The amylase was purified 14.6-folds to homogeneity by salting out with ammonium sulfate, and by some chromatographic procedures using DEAE-Toyopearl, Sephadex G-75, and hydroxyapatite. The molecular weight of purified enzyme was estimated to be 50.000 by SDS-polyacrylamide gel electrophoresis analysis. The optimum temperature and pH of the amylase was 500 and 6.0, repectively. The amylase activity was stimulated by the addition of Na+ ion, whereas it was inhibited by 10 mM Al3+, Cu2+ ions, and 1 mM EDTA. Enzyme inhibitors, such as the ß-mercaptoethanol, concanavalin A, hydoroxylamine-HCl, and acetic anhydride, did not affect the amylase activity. Maltotetraose was detected as the major product during the soluble starch hydrolysis, and small amounts of maltopentaose. maltotriose, and maltose were also found. The general properties like pH, temperature dependences, and starch hydrolysis patterns of Bacillus-expressed amylase maintained the original properties of the s albus KSM-35 amylase.
김재찬,김철위 大韓齒科器材學會 1990 대한치과재료학회지 Vol.17 No.2
The purpose of this study was to evaluate the characteristics of eight commercially available chemically activated composite resins (CT, HP, LC, PFQ, MR, SR-R, P-10, and BF-II) and ten light activated comkposite resins (BFM, DR, GT, LFA, SL, SP, AD-II, BF-I, EP and LFP) being using in Korea. The following properties were studied: surface hardness, diametral tensile strength, surface roughness, solubility and water sorption, differential thermal analytical properties. These tests were performed according to the modified methods of ISO 4049 and ANSI/ADA Specification No. 27. From the experiments, the following results can be drawn: 1. The surface hardness value of the chemically activated composite resins showed considerable differences among to the products, but there was no significant differences among the light activated composite resins. 2. The diametral tensile strength of the composite resins showed considerable difference among to the products, and the light activated composite resins showed a higher strength than that of the chemically activated resins. 3. Surface roughness values (Ra) and the maximum peak/valley values (Rtm) for the light activated composite resins showed considerably lower values, and the higher values was attained for the chemically activated resins. 4. Water sorption of the light activated composite resins was considerably higher than that of the chemically activated resins, and the solubility of the light activated composite resins was significantly higher than that of the chemically activated resins. 5. The slightly exothermic temperature curves of the composite resins proceeded at 150℃∼188℃, and the broad endothermic temperature curves were appeared at 380℃∼494℃.
정재위 한국세무회계학회 2003 세무회계연구 Vol.13 No.-
오늘날에는 자본시장의 급속한 발전으로 기관투자자의 투자결정에 필요한 회계정보의 수요가 확대되었다. 따라서 정보공시도 과거의 형식적인 공시에서 벗어나 기관투자자가 이해할 수 있고 투자목적에 적합하여 신뢰할 수 있는 정보의 공시가 제시되어야 한다. 본 연구는 이에 부합하여 현재의 기관투자자에 관한 정보에 대한 반응이 차이가 있는지를 알고자 한다. 기관투자자가 투자의사결정 시 어떤 접근방법을 활용하고 각 접근방법을 활용하는 요인이 무엇인지를 확인하며, 투자의사결정에 포함되는 정보집합(information set)이 무엇인지 추출하여, 회계정보가 투자의사결정시에 어느 정도 활용되며, 활용되는 정도를 결정하는 요인으로서 회계정보의 질적 속성을 비롯한 여러 변수들을 검정하는데 있다. 이와 같은 연구는 기관투자자가 활용하는 정보 집합 중에서 회계정보가 어느 정도 활용되는지를 확인하여 회계정보의 유용성을 결정하는 변수들을 밝힘으로써 회계정보의 유용성제고에 도움이 될 것이다. 특히 자본시장에 근거한 회계정보의 유용성 검정에 집중되어온 연구성과에 추가적으로 회계정보의 유용성을 확인하는 증거가 될 수 있을 것이다. 그리고 회계정보에 대한 이해가능성은 보통과 약간 높은 수준사이에 있으며 목적적합성은 보통 정도이고 신뢰성은 보통 이하로 나타났다. 이해가능성이 높을수록 목적적합성을 높게 인식하고 있으며 특히 손익계산서와 대차대조표에 대해서는 이해가능성에 불문하고 신뢰성이 매우 낮은 것으로 나타났다. This thesis examines the extent to which institutional investors utilize accounting information in their investment decision making. Based on prior researches and theoretical aspects, three groups of hypotheses are developed : ⑴ the degree of the utilzation of accounting information depends on the expected holding period of stocks and the approached to analyze their intrinsic value, ⑵ relevance and reliability are determinants of the degree of utilization of accounting information, and ⑶ accounting information is an alternative source of economy wide and industry specific information. Questionnaires collected from 123 institutional investors are analyzed. Statistical methods used are : descriptive statistics, X² test, t test. correlation analysis, analysis of valiance. regression analysis, and nonparametric statistics when applicable. The results indicate that ⑴ institutional investors utilize five information sets above average, while accounting information is relatively less utilized, ⑵ the low level of utilization of accounting information is due to low reliability, ⑶ there exists a difference in analysis of intrinsic value of stocks depending on expected holding period, ⑷ the higher the understandability, the higher the relevance of accounting information, while reliability has no significant relationship with understandablity, ⑸ accounting information is a complementary information with other information sets. The contribution of this thesis is to provide an evidence on the utilization of accounting information whcih is a major vehicle to ensure transparent management practice. Also, the low degree of utilization of accounting information is stemmed from low reliazbility on accounting information. This implied that upgrading the reliability of accounting information is the most urgent subject to pursue by accounting society. This has some limitations as are cases of other studies. The limitations are mostrey from the nature of questionnaire study which is employed in this thesis. There many by several extions of this study. It may be possible to examine the determinants of low level of reliability of accounting information including verifiability, representational faithfulness, and neutrality. Similar type of research can be replicated on the period after IMF vailout because of dramatically changed investment environments.
콤포짓트 레진의 중합체계에 따른 중합률 및 잔류단량체 유출
문현정,임범순,이용근,송재경,김철위 대한치과기재학회 2001 대한치과재료학회지 Vol.28 No.2
Newly developed curing units for the dental composite resins are claimed to result in optimum properties and short curing time. The purpose of this study was to detemine the curing effectiveness of the curing units, and to evaluate the relationship between the degree of polymerization and leachability of residual monomer. Three composite resins were tested (Z100; z100, Herculite XRV; HX, Heliomolar; HM). Disk specimens of 2 mm in thickness and 6 mm in diameter were cured with a plasma arc [Apollo 95E; at 1370 mW/㎠, for 5 sec.(A5), 10 sec.(A10), 15sec(A15)], halogen lamp [VIP; at 500 mW/㎠, for 13 sec.(V13), 26 sec.(V26), 40 sec.(V40)] and custom made light emitting diode [LED; at 500 mW/㎠, for 13 sec.(L13), 26 sec.(L26), 40 sec.(L40)]. Specimens were immersed in 75% ethanol for 7 days. Eluates of the composites were analyzed by a high-performance liquid chromatography, and the degree of polymerization of composites were determined by a Fourier transform infrared spectroscopy. To obtain the sufficient curing by a plasma arc, the curing time should be longer than 10 sec. When the same light energy was irradiated, LED showed similar curing performance to halogen lamp. The light energy and the degree of polymerization was not correlated (p>0.05), but the light energy and the leachability of residual monomer was correlated (p<0.05). The leachability of residual monomer (TEGDMA+BisGMA) depended on the degree of polymerization.
( Jae We Cho ),( Han Won Ryu ),( Sung Ae Kim ),( Hajime Nakano ),( Kyu Suk Lee ) 대한피부과학회 2014 Annals of Dermatology Vol.26 No.6
Epidermolysis bullosa simplex (EBS), an inherited genetic disorder, is most often caused by a dominant-negative mutation in either the keratin 5 (KRT5) or the keratin 14 (KRT14) gene. These keratin mutants result in a weakened cytoskeleton and cause extensive cytolysis. It is important to analyze the KRT5 or KRT14 genes of the patient and their family members by mutational analysis in order to identify genetic defects as well as the need for genetic counseling. In this study, we present a 5-year-old Korean boy who had been developing blisters and erosions on the palms of his hands and soles of his feet since infancy. In addition, while his younger sister and father showed similar clinical manifestation, his mother did not. The patient was diagnosed with EBS based on clinical manifestation, which is characterized by the presence of blisters restricted to the palms and soles, histological findings, and mutational analysis. Mutational analysis of the patient’s DNA revealed a thymine-to-cytosine transition at codon 608 in the KRT-5 gene, resulting in a leucine-to-proline substitution in the keratin 5 protein. The same mutation was identified in the paternal, but not maternal, DNA. Here, we report a case of Weber-Cockayne type EBS with vesicles and bullae restricted to the palms and soles with a novel, paternally inherited mutation in KRT5 gene (exon2, c.608T>C). (Ann Dermatol 26(6) 739∼742,2014)