Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells

( Jae-sung Ryu ),( Kyu-tae Chang ),( Ju-taek Lee ),( Malg-um Lim ),( Hyun-ki Min ),( Yoon-ju Na ),( Su-bin Lee ),( Gislain Moussavou ),( Sun-uk Kim ),( Ji-su Kim ),( Kinarm Ko ),( Kisung Ko ),( Kyung- 생화학분자생물학회(구 한국생화학분자생물학회) 2017 BMB Reports Vol.50 No.6

The ACKNOWLEDGEMENTS should be corrected as follows, "This research was supported by a grant (Code# PJ0074922012) from the Korean Rural Development Administration, and has been supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education Science and Technology (2010-0022316 and KCG5401011), and the KRIBB/KRCF Research Initiative Program (NAP), Korea. The published article in this journal was a part of the 2013 Doctoral thesis of Jae-Sung Ryu submitted to Wonkwang University, Korea." and not "This research was supported by a grant (Code# PJ0074922012) from the Korean Rural Development Administration, and has been supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education Science and Technology (2010-0022316 and KCG5401011), and the KRIBB/KRCF Research Initiative Program (NAP), Korea."

Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

결핵항원에 의한 결핵환자 말초혈액 단핵구의 IL-2,IL-10 및 TNF-α mRNA 발현비교

박정규,임영재,김화중,조은경,민들레,임재현,최덕례,박성규 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

The various clinical features of tuberculosis are mediated by diverse cytokines produced by various immune cells which are initially triggered by M. tuberculosis antigens. CD4+ T cells can be classified into two subsets according to the patterns of cytokines they produce; Thl cells give rise to cell-mediated immunity and are characterized by the production of IL-2 and IFN-y, whereas Th2 cells are more efficient in mediating antibody production and secrete II-4, IL-5, IL-6 and II-10, Thl cells can control Th2 cell and vice versa. Peripheral blood mononuclear cells of healthy, cured and chronic refractory tuberculosis patients were stimulated with PPD, TSP and PHA antigen, and lymphoproliferative response and expression of II-2, IL- 10, TNF-α mRNAs were measured. Lymphoproliferative responses to PPD, TSP and PHA antigen were depressed in chronic refractory case compared with others expression of IL-2 mRNA was depressed in chronic refractory case stimulated with all antigens. Expression of IL-10 and TNF-α were depressed in cured and chronic refractory cases stimulated with PPD and TSP antigens.

이화방어기제검사 표준화연구

김재은,김정규,박영숙,이근후 大韓神經精神醫學會 1991 신경정신의학 Vol.30 No.3

This study aimed at an evaluation of the reliability and validity of Ewha Defense Mechanism Test(EDMT) for standardization during the period from June 25 to July 10 with nationalwide sample which was standardized by Rhee Kun Hoo. Kin Jae Un. Kim Jung Kyu. and Park Young Sook. The number of subjects were 1.323 who were sampled by the method of proportional stratification on age. sex. education. local area variables. This test is composed of 20 defense mechanism subscales and total items of the test are 200. The results were as follows : 1) The range of means of each scales were from 2.70 to 3.34 and the range of items standard deviation was from .71 to 1.28. 2) The corrected item-total correlation coefficients were than .10 for all items except two items which was the index of internal consistancy. 3) The spearman-Brown split-half reliability coefficients were more than .66 and the retest reliability coefficients were more than .72 for all scales. 4) By the evaluation of the cross validation with “16 Personality Factor Inventory”. it was found that 11 factors were consistent with expectation among 16 factors so that the result of cross validity was moderately sufficient. 5) By the factor analysis. four factors were extracted. Factor 1 was “unstable sensitization defense mechanisms”which were acting out, displacement, somatization. dissociation. projection. and passive-aggressive behavior. Factor 2 was “ego-expansive defense mechanisms” which were composed of controlling. distortion. altruism. humor. and sublimation. Factor 3 was “ego-denial defense mechanisms” which were reaction formation. bragging identification. and regressin. Factor 4 was “reality evasive defense mechanisms” which were denial rationalization. suppression. anticipation. and evasion.

경구용 세팔로스포린계 항생제 Cefditoren pivoxil의 주요 임상분리세균에 대한 시험관내 항균력

이종윤,이재규,김의종 대한감염학회 2003 감염과 화학요법 Vol.35 No.4

목적 : Cefditoren은 경구용 세팔로스포린제로서, 지역사회 획득 폐렴의 주요 원인균인 H. influenzae, S. pneumoniae와 M. catarrhalis에 대하여 우수한 항균력을 갖고 있다고 국외에서 보고되었다. 본 연구는 최근 우리 나라에서 분리된 주요 임상분리세균을 대상으로 Cefditoren pivoxil에 대한 최소 억제농도를 측정하여 Cefditoren pivoxil의 항균제 감수성 양상을 파악하는데 그 목적이 있다. ㎍/㎖방법 : 임상환자의 검체로부터 2002년에 분리한 총 504개의 임상균주를 대상으로 미국임상검사표준화협회의 방법에 따라 MIC를 측정하였다. E. coli, K. pneumoniae, E. cloacae, C. freundii, S. marcescens, P. mirabili와 Staphylococcus spp.의 경우 128㎍/mL에서 0.03㎍/mL까지의 범위내에서 배수희석한 한천배지희석법으로 MIC를 측정하였으며, H. influenzae, S. pneumoniae와 M. catarrhalis의 경우 16㎍/mL에서 0.015㎍/mL까지의 범위 내에서 배수희석하여 액체배지희석법으로 MIC를 측정하였다. 결과 : Enterobacteriaceae에 대한 cefditoren의 항균력은 균종에 따라 다양하게 나타났다. E. coli와 P. mirabilis에 대한 MIC90는 각각 8㎍/mL과 0.5㎍/mL으로서 비교적 낮았다. 그러나 K. pneumoniae, E. cloacae, C. freundii와 S. marcescens의 경우 cefditoren의 MIC_(90) 범위가 32㎍/mL에서 >128㎍/mL으로 비교적 높았다. MSSA와 MSSE에 대한 cefditoren의 MIC_(90)는 각각 1㎍/mL와 0.5㎍/mL이었다. Cefditoren은 S. pneumoniae에 대하여 penicillin의 감수성 여부에 관계없이 MIC_(90)가 1㎍/mL이었고, H. influenzae와 M. catarrhalis에 대하여 MIC_(90)가 각각 0.03㎍/mL와 0.5㎍/mL이었다. 결론 : Cefditoren은 Enterobacteriaceae에 대하여는 다양한 항균력이 관찰되었으나, S. pneumoniae, H. influenzae와 M. catarrhalis에 대하여 우수한 항균력을 보였으므로, 호흡기 감염을 일으키는 주요 원인균에 대하여 항균력이 우수함을 알 수 있었다. 따라서 cefditoren은 지역사회 획득 폐렴을 치료하기 위한 경구용 항균제로 유용할 것으로 생각한다. Background: Cefditoren is an oral cephalosporin with excellent activity against Haemophilus influenzae, Streptococcus pneumoniae. and Moraxella catarrhalis, which are the predominant bacterial causes ot community-acquired respiratory tract infections. The current study attempted to determine the antibacterial activity of cefditoren against the major clinical isolates. Methods: According to the NCCLS recommendations, antibacterial activities of cefditoren were measured against total 504 major clinical isolates. MICs were determined by the agar dilution method, a series of doubling dilutions from 128 to 0.03 ㎍/mL, on E. coli. K. pneumoniae, E. cloacae. C. freundii, S. marcescens. P. mirabilis, and Staphylococcus spp. In case of H. influenzae. S. pneumoniae, and M. catarrhalis, broth microdilution method, a series of doubling dilutions from 16 to 0.015 ㎍/mL, was performed. Results: Cefditoren had variable activity against Enterobacteriaceae. MIC cumulative curves showed that cefditoren had low MIC distributions against E. coli and P. mirabilis, and MIC_(90) were 8 and 0.5 ㎍/mL, respectively. Against K. pneumoniae, E. cloacae, C. freundii, and S. marcescens, cefditoren's MIC_(90) values ranged from 32 to >128 ㎍/mL. For clinical isolates of methicillin-susceptible S. aureus and methicillin-susceptible S. epidermidis, cefditoren had MIC_(90) values of 1 ㎍/mL and 0.5 ㎍/mL, respectively. Cefditoren had MIC_(90) values of 1 ㎍/mL for penicillin-susceptible and penicillin-not-susceptible strains of S. pneumoniae. Cefditoren had MIC_(90) values of 0.03 ㎍/mL and 0.5 ㎍/mL against H. influenzae and M. catarrhalis, respectively. Conclusion: Cefditoren had excellent activity against S. pneumoniae, H. influenzae, and M. catarrhalis. Cefditoren had variable activity against Enterobacteriaceae. The results of this study confirm the excellent activity of cefditoren against the major respiratory tract pathogens and suggest that cefditoren could be a good antibiotic for empiric oral treatment of community-acquired respiratory tract infections.

Effects of epigallocatechin gallate on CoCl_(2)-induced apoptosis in PC12 cells

Yang, Kyu-Ho,Mo, Hyun-Chul,Choi, Nam-Ki,Kim, Seon-Mi,Kim,Won-Jae 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

Neuronal apoptotic events, consequently resulting in neuronal cell death, are occurred in hypoxic/ischemic condition. This cell death has been shown to be accompanied with the production of reactive oxygen species (ROS), which can attack cellular components such as nucleic acids, proteins and phospholipid. However, the underlying mechanisms of apoptosis induced in hypoxic/ischemic condition and its treatment methods are unsettled. Cobalt chloride (CoCl_(2)) has been known to mimic hypoxic condition including the production of ROS. Epigallocatechin gallate (EGCG). a green tea polyphenol, has diverse pharmacologial activities in cell growth and death. This study was aimed to investigate the apoptotic mechanism by CoCl_(2) and effects of EGCG on CoCl_(2)-induced apoptosis in PC12 cells. Administration of CoCl_(2) decreased cell survival in dose- and time-dependent manners and induced genomic DNA fragmentation. Treatment with 100 µM EGCG for 30 min before PC12 cells were exposed to 150 µM CoCl_(2), being resulted in the cell viability and DNA fragmentation being rescued. CoCl_(2) caused morphologic changes such as cell swelling and condensed nuclei, whereas EGCG attenuated morphologic changes by CoCl_(2). EGCG suppressed the apoptotic peak and a loss of Δψ_(m) induced by CoCl_(2). CoCl_(2) decreased Bcl-2 expression but Bax expression was not changed in CoCl_(2)-treated cells. EGCG attenuated the Bcl-2 underexpression by CoCl_(2). CoCl_(2) augumented the cytochrome c release from mitochondria into cytoplasm and increased caspase-8, -9 and caspase-3 activity, a marker of the apoptotic executing stage. EGCG ameliorated the incruement in caspase-8, -9 and -3 activity, and cytochrome c release by CoCl_(2). NAC (N-acetyl-cysteine), a scavenger of ROS, attenuated CoCl_(2)-induced apoptosis in consistent with those of EGCG. These results suggest, that CoCl_(2) induces apoptotic cell death through both mitochondria- and death receptor-dependent pathway and EGCG has neuroprotective effects against CoCl_(2)-induced apoptosis in PC12 cells. 신경세포자멸사는 저산소 및 허혈환경에서 일어나며 이러한 세포죽은 reactive oxidant species (ROS) 생성을 동반함이 알려져있다. 그러나, 저산소 및 허혈환경에서 일어나는 세포자멸사의 기전 및 그 치료방법은 아직 정립되어 있지 않다. CoCl_(2)는 ROS를 생성하는 등 저산소환경과 유사한 조건을 초래하는 것으로 알려져 있다. Epigallocatechin gallate (EGCG)는 녹차의 polyphenol 성분으로서 세포성장과 죽음에 다양한 약리학적 효과를 나타냄이 알려져 있다. 본 연구는 PC12 세포에서 CoCl_(2)에 의한 세포자멸사기전을 밝히고 이에 미치는 EGCG의 효과를 조사하는데 목적이 있다. Cell viability는 MTT 측정으로 조사되었고, DNA fragmentation은 DNA laddering으로 조사되었다. Bcl-2와 Bax발현 정도는 RT-PCR로, caspase-3와 -9의 활성은 spectrophotometer, caspase-8의 활성은 flow cytometry에 의해 측정되었다. 미토콘드리아에서 세포질로 분비된 cytochrome c는 western blot으로, 분해된 DNA 양과 미토콘드리아 세포막전위 (Δψ_(m))는 FACScan으로 조사되었다. CoCl_(2)투여로 PC12 세포수는 용량 및 시간 의존형태로 감소하였고, genomic DNA fragmentation이 발생하였다. CoCl_(2)투여로 야기된 cell viability의 감소와 DNA fragmentation은 EGCG 전처치에 의해 억제되었다. CoCl_(2)은 세포용적팽창과 condensed nuclei 같은 형태적 변화를 일으켰으며, apoptotic peak, Δψ_(m)감소 및 cytochrome c 유리를 야기하였다. EGCG는 CoCl_(2)에 의한 세포형태변화, apoptotic peak, Δψ_(m)소실 및 cytochrome c 유리를 억제시켰다. CoCl_(2)는 Bcl-2 발현을 감소시켰지만, Bax 발현에는 영향을 미치지 않았다. EGCG는 CoCl_(2)에 의해 야기된 Bcl-2 발현 감소를 억제시켰다. CoCl_(2)는 caspase-3, -8, 그리고 -9의 활성을 증가시켰으며, EGCG는 CoCl_(2)에 의한 세포자멸사를 억제시켰다. 본 실험결과는 PC12 세포에서 CoCl_(2)가 미토콘드리아 의존 및 death receptor의존 기전으로 세포자멸사를 일으키며, EGCG는 세포자멸사기전을 억세지킴으로 신경보호기능을 가짐을 시사하였다.

체적제어를 이용한 신뢰성 기반 위상 최적화

박재용,황승민,임민규,오영규,박재용,한석영 한국공작기계학회 2009 한국공작기계학회 춘계학술대회논문집 Vol.2009 No.-

This paper presents a reliability-based topology optimization (RBTO) using bi-directional evolutionary structural optimization (BESO). An actual design involves uncertain conditions such as material property, operational load and dimensional variation. Deterministic topology optimization (DTO) is obtained without considering of uncertainties related to the uncertainty parameters. However, the RBTO can consider the uncertainty variables because it has the probabilistic constraints. In this paper, the reliability index approach (RIA) is adopted to evaluate the probabilistic constraint. RBTO based on BESO starting from various design domains produces a similar optimal topology each other. Numerical examples are presented to compare the DTO with the RBTO.

Low Density Lipoprotein의 실측치와 계산치에 관한 조사

김언규,정재린,이경옥,서덕규,이규범 대한임상병리사협회 1993 대한임상병리사회지 Vol.25 No.1

가족성 용종증에서 시행한 J형 회장 저장낭-항문 문합술의 수술성적

이현국,박규주,오재환,박재갑,이현국,박규주,오재환,박재갑 대한대장항문학회 1995 Annals of Coloproctolgy Vol.11 No.4

A. 高校 科學 (生物·地球科學) 및 數學科 探究學習의 評價模型 定立과 評價紙 開發

李相協,權炳奎,林在圭,孫文求,吳岱燮,楊洪準,梁承榮,姜用熙,林成圭,金正遠,金吉民,兪正在 경북대학교 과학교육연구소 1982 科學敎育硏究誌 Vol.6 No.-

Currently the evaluation of high school science and mathematics learning has been too much knowledge-centered without concerning the inquiring ability or scientific attitude. This study, therefore, was made for the purpose of establishing of evaluation model for high school science (biology and earth science) and mathematics learning and its evaluation items. In this paper, the research was done about three fields, biology, earth science and mathematics. 1. For the biology, the evaluation methods of inquiry learning were studied with the foreign references, and proper evaluation items were suggested. 2. For the earth science, after analizing 5 kinds of earth science textbook of current using in high school, essential 19 items were selected. 5 of them were tested in test group and control group, then, the results were analized. 3. For the mathematice, the teaching elements of「Set」and「Flow chart」in mathematics (Ⅰ) were selected and structured. Detailed teaching objects were established and evaluation items were introduced.