Priming 처리종자의 저장기간에 따른 발아력 전당함량 및 α-amylase 활력

김재현,이석순,박성태,정명근 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.3

벼 종자의 priming처리 후 저장성을 검토하기 위하여 저장기간과 저장온도에 따라서 종자의 발아력과 저장양분 및 발아에 관여하는 주요 효소활력의 변화를 조사한 결과를 요약하면 다음과 같다. 1. Priming한 종자를 6개월간 저장하여도 어느 저장온도에서나 발아율의 차이가 없었다. 2. Priming한 종자에서 발아속도(T_50)가 2∼3일 빨랐으며 3개월 동안은 변화가 없었고 3개월 이후 발아속도가 약간 감소하였으나 priming 처리하지 않은 종자에 비해서 빨랐다. 3. 발아균일도는 priming 처리효과가 6개월간 계속 유지되었으며 25℃보다 저온인 17℃에서 효과가 더 크게 나타났다. 4. α-amylase 활력은 저장기간이 길수록 높았고 전당함량의 경우 3개월 이상 저장하였을때 감소하였으나 발아력에는 영향을 미치지 않았다. 5. 종자 수확후 6개월 동안은 어느 시기에 priming 처리하여 저장하여도 발아력 및 종자의 활력은 유지되었다. This experiment was carried out to determine changes of seed germination ability, total sugar, and α-amylase activity to different storage period, and temperature after seed priming treatment. In the storage during the six months, final germination rate and speed were similar to the untreated control seeds and the uniformity was continuously preserved. Germination speed(T_50) was faster than the control seeds by 2∼3 days. T_50 was a little decreased to priming effect after three months but faster than the control seeds. In the case of germination temperature 25℃, germination uniformity was decreased to long term storage periods. Total sugar content was increased to compare with the control seed during the one months at -10, 5 and 20℃ and total sugar content was decreased after two months. α-amylase activity was increased to storage of long term period and seed of storage temperature 5℃ was increased compared with -10 and 20℃. Germination ability and α-amylase activity was preserved continuously during six months after priming treatment.

Medx 운동치료 프로그램이 경추근력과 통증에 미치는 효과

남덕현,이한경,박재우,김명준 龍仁大學校 2000 용인대학교 논문집 Vol.18 No.-

The purpose of this study was to evaluate the improvement of the cervical extensor muscles strength in a 103 patients with laser discectomy using Medex program. 103 Patients was divide into 3 Groups, Study group of 40 patients (M=17, F=23), Control Group of 32 patients (M=17, F=15), Normal Group of 31 patients(M= 19, F= 12) Study and Control group was the patient of Laser discectomy on the cervical disc. Study Group was trained with Medx exercise program for the cervical muscles for 12 weeks after 1 month from cervical Laser discectomy. Control Group was rest at home for 12 weeks after the therapeutic exercise program. Normal group was good condition normal persons in cervical and Lumber spine without lesion, 31 persons was tested cervical extension muscle strength before and after 12 weeks. The results of this study were as follows ; 1. In initial muscle strength. The male was found 34275.95 8546.55(n=20) in study croup, 32279.66 3965.43(n=18) in control group, 54297.42 721,39(n=19) in Normal group. The female was found 24445.25 7360.80(n=20) in study group, 25125.64 4463.65(n=14) in control group, 42829 9649.62(n=12) in normal group. Therefore in a study and control group, It was found that the subjects have similar muscle strength after the 12 weeks with no difference. 2. Between three group, after 12 weeks, improvement of male muscle strength was found 50142.29 13017.37(n= 17, study group), 38973.44 7424.36 (n=18, control group), 55774.41 9268.05 (n=17, Normal group) in male. And improvement of female muscle strength was found 40227.80 6062.89(n= 15, study group), 29777.78 10647.34 (n=14, control group). As a result, muscle strength after 12weeks, it was found a little difference cervical extension muscle strength in control group without therapeutic Exercise program. But study group there was a much difference. 3. Study group was statistically significant difference(P<.05, n=40) after 12weeks. And control group was statistically significant difference, too(P<.05, n=32). But in real value, improvement of muscle strength was 50% improvement in study group more than 19% in control group. 4. After 12weeks in a study and control group, Pain scale shows statistically significant difference in study group than control group(P<.05). 5. After 12Wks, Cervical ROM was increased in a study and control group, each other. In a Study group it was found that there was an increase ROM more than control group(Flexion position; study/control G. =124.6 /118.8, Extension position: =1.68 /8.4, ROM; full Extension=0, full Flexion= 126). In conclusion, After 1 month laser discectomy in cervical HNP, The study group with procedure of Medx therapeutic exercise program was statistically significant increased cervical extension muscle strength more than control group with no therapeutic exercise program. And compared with control group, Study group shows decrease in a pain scale as well as the ROM of cervical mobility. Therefore, during 12 weeks Medx therapeutic Exercise in the cervical spine was effective in cervical muscle strength and for the pain, limited ROM, and dysfunction. And the early recurrent and prevention is positively necessary scientific controlled therapeutic exercise program.

問項反應理論을 適用한 TENNIS FORHAND STROKE의 難易度

남덕현,성락민,정재윤 龍仁大學校 1997 용인대학교 논문집 Vol.13 No.-

The purpose of this study was to identity the applications of rating scale toward an evaluation of tennis forhand stroke. The ability test of tennis volley presented in AAHPERD research, Rasch Model, was adapted and it's rate point was used in FACETS (lincere, 1994). First, The functional ability in tennis stroke was aimed to be efficiently by using RASCH'S rating scale based on six positions. Test difficulties were shown between -0.15 to 0.15. The easiest position amonsg those 6 was #3(-0.15) and #2(-0.09) in order and the most difficult position was known as #4(0.15). Second, a logit point of subjects was shown in difference when logit average was set on zero. Test 2,3,4, and 5 were revealecl to be valid as a stroke test in which subject's functional ability of tennis forehand stroke coned be identified. Third, the difficulty rate based on position was ordered by #1, #3, and #6 position as low change position #4 and #5 as high change Finally, the positional test in 6 position' base was regarded as a valid test method to evaluate tennis forehand stroke. Rasch's rating scale model was identified as a well appllied model to measure the difficulty rate in tennis forehand stroke.

동등백분위법을 이용한 순발력 검사 방법간의 동등화 방안

남덕현,윤상화,김도준,박재우 龍仁大學校 體育科學硏究所 1999 體育科學硏究論叢 Vol.9 No.1

This paper is designed to equalize the points obtained from different tests of agility by means of equipercentile equating in accordance with grade and sex. The subjects consist of 923 high school students who have ever gone through the tests of power. As a result, I have reached the following conclusion. 1. I used equipercentile equating for recording the tests of power of the first male graders, coming up with equalized values such as 223㎝ in a standing board jump, 33.7m in five seconds' running and 42m in throwing, and 53m in a vertical jump at the 50 percent level. 2. The second male graders, coming up with equalized values such as 232㎝ in a standing board jump, 33.5m in five seconds' running and 42m in throwing, and 57㎝ in a vertical jump at the 50 percent level. 3. The third male grades, coming up with equalized values such as 242㎝ in a standing board jump, 34.8m in five seconds' running and 45m in throwing, and 57㎝ in a vertical jump at the 50 percent level. 4. The first female graders, coming up with equalized values such as 168㎝ in a standing board jump, 25.8m in five seconds' running and 10m in throwing, and 34㎝ in a vertical jump at the 50 percent level. 5. The second female grader, coming up with equalized values such as 174㎝ in a standing board jump, 25.8m in five seconds' running and 17m in throwing, and 31㎝ in a vertical jump at the 50 percent level. 6. The third male graders, coming up with equalized values such as 187㎝ in a standing board jump, 27m in five seconds' running and 16.6m in throwing, and 30㎝ in a vertical jump at the 50 percent level.

大豆油 高溫處理時 BHA, TBHQ, Silicone 및 시트르산 添加가 品質에 미치는 영향

全濟鉉,李應昊,金世權 釜山水産大學校 1984 釜山水産大學 硏究報告 Vol.24 No.1

튀김할 때 식용유는 가열매체인 동시에 풍미나 영양원이 된다. 튀김용 식용유의 품질변화방지를 위한 기초자료를 얻을 목적으로 식용대두유로서 고온처리 및 튀김을 할 때 BHA, TBHQ, silicone 및 시트르산 첨가가 식용유의 품질에 미치는 영향을 실험하였다. 98℃이하의 열처리 결과 시트르산 1ppm, TBHQ는 50∼200ppm첨가한 것이 산화방지에 효과적이었으나 BHA와 silicone은 효과가 거의 없었다. 180℃ 가열처리 및 튀김시험에 있어서는 시트르산은 산화를 촉진하는 경향이 있었고 BHA 및 TBHQ는 별 효과가 없었으며 silicone이 가장 효과가 좋았다. silicone 1∼5ppm가지는 품질변화방지효과에 큰 차이가 없었으나 튀김할 때는 원료에 이행하는 양이 있으므로 첨가량이 증가할수록 효과가 좋았다. 그러므로 식용유 품질변화를 방지하는데 있어 100℃이하에서 처리할 때는 시트르산을 첨가하는 것이 효과적이고, 튀김처럼 고온처리하는 식용유에서는 1∼5ppm의 silicone을 첨가하는 것이 효과적이라는 결론을 얻었다. Edible oils serve both as a heat transfer medium and a source of flavor and nutrition since ,some of the fat is absorbed by the food being fried. In this paper, the effects of butyl hydroxyanisole(BHA), teritary butyl hydroquinone(TBHQ), silicone and citric acid on thermal stability of the soybean oil during heating and frying were evaluated. Judging from the results of swift stability test (active oxygen method) that the minimum effective concentration of citric acid and TBHQ for ,the thermal stability of soybean oil when heated at 98℃ was 1 ppm and 50 to 200 ppm, respectively. But BHA and silicone showed no effect. On the other hand, silicone seemed to improve the thermal stability of soybean oil during heating at 180℃ or frying with potato while BHA and TBHQ showed no significant effect.

방광암에 특이한 RIBONUCLEASE 의 작용기전에 관한 연구

나현재,김동한,고재경 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

The neutral RNase isozyme with the highest activity isolated from bladder cancer tissue by an ion exchange column chromatography was shown to be specific to bladder cancer by means of analysis of high performance liquid chromatography(HPLC ) and polyacrylamide gel electrophoresis (PAGE) for the enzyme proteins. Also studied were substrate specificity and the product analysis of the RNase to investigate the role of the enzyme in pathogenesis of the bladder cancer. Neutral RNase in the bladder cancer tissue was separated by a DEAE-cellulose column chromatography into 3 peaks and a single neutral RNase isozyme present in the control bladder tissue disappeared from the cancer tissue. The substrate specificity of the peak Ⅴ neutral RNase isozyme with the highest activity among the RNases isolated from the bladder cancer tissue appeared to be different from that from the control bladder tissue. HPLC and PAGE patterns of proteins complexed with the peak Ⅴ RNase from the cancer tissue also appeared to be different form that the peak Ⅴ neutral RNase was specific to the bladder cancer. The peak Ⅴ neutral RNase isozyme from the bladder cancer tissue was not active toward ds polyribonucleotide, but active toward ss polyribonucleotide, principally catalyzing the hydrolysis of A-C, C-C A-U, linkages. The fact that majority of products of poly C digest by the RNase was oligoribonucleotide indicated that 1) the peak Ⅴ neutral RNase isozyme from the bladder cancer tissue was specific to the cancer, 2) the RNase isozyme was active toward ss polyribonucleotides (active toward A-C, C-C A-U linkages) and 3) the enzyme was endoribonuclease in nature, suggesting that the enzyme was suppressive to the bladder cancer in action.

汚加皮 抽出液이 마우스의 抗腫瘍 免疫反應에 미치는 影響

成載煥,權文鉉,金炯均 圓光大學校 韓醫學硏究所 1995 원광한의학 Vol.5 No.1

Ogapi(Acanthopanax senticosus) is being used for a tonic in the oriental medicine from ancient times, Even though it being used frequently for a tonic in the clinical medicine, basic study to make sure the mechanism of its action is the rare. Therefore, in this study, the author tried to know the effects of ogapi on the innate and specific immune systems in association with antitumor activity. The results obtained in this study are as follows: 1) Ogapi increased the phagocytic activities of murine peritoneal macrophages in vivo and in vitro. 2) Ogapi enhanced the production of such reactive oxygen intermediates as superoxide and hydrogen peroxide from the macrophages in vivo and in vitro. 3) Ogapi enhanced such humoral immune responses as hemagglutinin and hemolysin formations. 4) Administration of ogapi increased the number of rosette forming cells in the spleen. 5) Ogapi decreased the contact hypersensitivity against dinitrofluorobenzene. 6) Ogapi showed cytotoxic activity against human leukemia cell line (K562) 7) Ogapi increased the ratio of helper T cell versus suppressor T cell by increasing CD4^+ T cells and decreasing CD8^+ T cells. The above results show that ogapi increase the host antimicrobic and antitumor activities by the action on individual innate and specific immune systems in vitro. So this is considered as experimental results back up powerfully that ogapi can be used for a tonic in such a case when administered to a person lacking vital energy.

소음 노출 남성 근로자의 고음영역 청력손실과 관련 요인

정상재,우극현,박완섭,유재영,최태성,김상우,김진석 대한산업의학회 2000 대한직업환경의학회지 Vol.12 No.2

Objectives : The purpose of the study was to assess the risk factors assumed to be related to the high frequency hearing loss in the noise-exposed male workers. Methods : 712 occupationally noise-exposed male workers were included in this cross sectional study. The subjects filled out a questionnaire on the history of noise exposure, hearing protection, otologic diseases, tobacco smoking, and were tested on height, weight, hematocrit, serum total cholesterol level, fasting plasma glucose level and arterial blood pressure level. Pure tone audiometry and otoscopy were conducted and the subjects were allocated into two groups according to whether they had 4000 Hz hearing loss or not. Results : The multiple logistic regression analysis showed that military noise exposure, poor wearing status of the hearing protection devices, increased age, long noise exposure duration, mean arterial pressure less than 80mmHg, high fasting plasma sugar are the factors significantly related to high frequency hearing loss (P〈0.05). On the contrary, there was no significant relationship between noise intensity, body mass index, serum total cholesterol level, smelling and high frequency hearing loss. Low hematocrit was shown to be a significant factor with a p-value less than 0.1. Conclusions : High frequency hearing loss in the noise-exposed male workers is related to military noise exposure, use of hearing protection devices, age, noise exposure length, fasting glucose level and mean arterial pressure, in this order. Appropriate use of the hearing protection devices has a great impact on the prevention of the noise-induced hearing loss.

직업적 디메틸아세트아미드(Dimethylacetamide) 노출에 의해 집단적으로 발생한 독성간염

최태성,우극현,김진석,박완섭,함정오,정상재,유재영 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.2

목적 : DMAC에 노출된 후 집단적으로 발생한 독성 간염 7례보고. 방법 : 2000년 2월 신설된 스판덱스 섬유제조 공장에 근무하는 생산직 근로자 178명중 2000년 2월 부터 8월까지 7명이 간장질환이 발생하였다. 환례들의 나이는 23∼47세였고, 남자 5명, 여자 2명이었다. 이들에 대해 혈액검사, 간 초음파 검사 등의 임상검사와 작업장 조사를 실시하였다. 결과 : 근무 부서는 중합 1명, 방사 1명, 권취 4명, 그리고 포장 1명이며 평균근무기간은 10주였다. 주 호소 증상은 심한 피로감, 어지럼증 및 황달이었고, 검사실 소견상 독성간염의 소견을 보였다. A, B 및 C형 간염 표지자 검사는 모두 음성이었고, 간초음파 소견상 특이 소견이 없었다. 문진 상 알코올 성 간염을 일으킬 정도의 음주력이나 최근 약물 복용력을 가지고 있는 환례는 없었다. 입원 후 보존적인 치료로 모두 급속히 호전되었다. 초기에 발생했던 환례에서 퇴원후 DMAC에 재 노출되어 독성 간염이 재발되었다. 이후 모든 환례들은 퇴원 후 DMAC 비노출 부서로 전환하였고, 퇴원후 지속적인 추적검사에서 간 효소 수치가 완전히 정상화되었고, 정상화되는 기간은 1∼2개월 정도 소요되었다. 결론 : 환례들의 임상증상, 검사결파, 노출력 및 과거력상 DMAC에 의한 독성간염으로 추정된다. Dimethylacetamide is widely used in the production of plasics, resins, synthetic fibers, and gums and in purification and crystallization processes. Inhalation of the vapor or skin absorption of the liquid of dimethylacetamide (DMAC) can cause liver damage. Toxic hepatitis possibly attributable to DMAC exposure occurred in seven works among 178 employees who had worked on a new spandex-fiber production line. A large amount of DMAC is used as a spinning solvent for synthetic fibers in the factory. The patients were aged 23-47 years old and composed of five males and two females. They were involved in the process of polymerization(1 patient), spinning(1), take-up(4) and packaging(1). The mean duration of exposure was 10 weeks. They experienced fatigue, dizziness and jaundice. The patients showed elavated total bilirubln, alanlne aminotransferase (ALT), and aspartate aminotransferase (AST) levels. The serologic test for viral hepatitis A, B and C were negative, as were the abdominal ultrasonographic scans. Based on the presumptive diagnosis of DMAC-induced toxic hepatitis, they were removed from the workplaces. One or two months after removal from the work, the transaminase levels returned to normal. The patients had no history of significant alcohol use, blood transfusion, recent medication, and drug abuse. As a result, authors could not find any attributable cause of toxic hepatitis but the toxicity by DMAC exposure.

트랙타 부착 대형이앙기 개발 및 재배적 실용성

박성태,장익주,정창화,김상열,김재현,문헌팔,양세준,김순철 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.3

쌀 생산비를 줄이고 대경지 규모화 영농에 적응할 수 있는 농기계 개발을 목적으로 연구개발한 대규모 경지적응 트랙터 부착 대형벼이앙기의 구성과 특징 및 개발시작기의 재배적 실용성 평가결과를 요약정리하면 다음과 같다. 1. 트랙터 부착 12조 대형벼이앙기는 크게 바퀴자국 제거로타리, 문짝형 정지판, 이앙장치부 등으로 구성되어 있다. 2. 트랙터 부착 12조 이앙기는 트랙터 동력을 이용하여 로타리, 정지 및 이앙작업이 동시에 이루어 질 수 있도록 개발하였다. 3. 바퀴자국을 없애는 날은 기존의 로타리 날을 교체하면 되도록 반달형 날로 제작하였다. 4. 이앙기는 이동시는 접을 수 있도록 설계 제작되어 대형화가 가능하므로 기존 6조 승용이앙기에 비하여 이앙작업 능력이 1.5배 이상이었다. 5. 트랙터 부착 대형벼이앙기는 트랙터와 같은 큰 동력원을 이용할 수 있으므로 측조시비기, 긴매트모 탑재 등 다양한 작업기 및 기능을 추가로 부착 가능하도록 설계되었다. 6. 트랙터 부착 대형벼이앙기는 어린모 및 중묘이앙시 결주율은 3.0∼4.3%로 기계이앙기 결주 허용범위 5% 미만이었다. 하지만 무경운 논에서는 이앙작업은 가능하나 부묘율이 다소 높은 점은 보완이 필요하다. To develop a labor saving rice cultivation technology, a new tractor attached rice transplanter has been developed for large scale farming and its field performance was evaluated. The rice transplanter consists of three major parts such as rotary for removing tractor tire furrow, door-shaped soil leveling plate and transplanting equipment. The rice transplanter is operated using the power of the tractor and performs rotary, harrow and seedling transplanting simultaneously. A half moon shaped rotary blade was found to be very efficient to remove tire furrow developed during seedling transplanting and the original rotary blade can be replaced easily with existed rotary blade. The seedling transplanting part of the transplanter was designed to fold into small size and the transplanting efficiency of the transplanter was found to be 1.5 times higher than that of the conventional riding type six row transplanter. Since the transplanter is operated through a strong power of the tractor, the additional attachment such as fertilizer application apparatus and long mat rice seedling carrier is possible. Based on the field performance of the transplanter, although the missing hill percentage showed roughly with 3.0∼4.3% for 10-day and 30-day old seedlings but the percentage is less than 5% which is maximum missing hill permit in the transplanting rice. Seedling transplanting in no-tillage field condition is acceptable but floating seedling percentage is still high and thus further technology improvement for minimizing floating seedling percontage is needed.