Cloning, Purification, and Characterization of a New DNA Polymerase from a Hyperthermophilic Archaeon, Thermococcus sp. NA1

( Yun Jae Kim ),( Hyun Sook Lee ),( Seung Seob Bae ),( Jeong Ho Jeon ),( Jae Kyu Lim ),( Yo Na Cho ),( Ki Hoon Nam ),( Sung Gyun Kang ),( Sang Jin Kim ),( Suk Take Kwon ),( Jung Hyun Lee ) 한국미생물생명공학회 2007 Journal of microbiology and biotechnology Vol.17 No.7

원위부 신세뇨관성 산증에서 산-염기 운반체의 결손

김혜영,한진석,이정상,김현리,김진,이중건,이서진,김근호,진호준,전은실,주권욱,나기영,정우경,오지은,엄재호,궁성수 대한신장학회 2000 Kidney Research and Clinical Practice Vol.19 No.5

The purpose of this study was to elucidate whether the molecular defect of acid-base transporters in renal tubules is related to the functional defect of urinary acidification in distal renal tubular acidosis(RTA). We performed NH₄Cl, furosemide, or bicarbonate loading test to evaluate renal acidification function, and immunohistochemistry using antibodies to H^+ -ATPase, Cl^-/HCO₃^- exchanger(band-3 protein), and Na^+/K^+ -ATPase in kidney tissue in 6 patients with RTA and renal cell carcinoma patients as normal controls. Kidney tissue was obtained either by percutaneous needle biopsy(RTA) or nephrectomy(NC). The results were as follows; 1) In all six RTA patients, proton secretory defect of distal acidification was shown by a failure to lower the urine pH after NHC1 loading or furosemide test or abnormally low urine-blood pCO₂ difference during bicarbonate loading. In two patients with RTA, proximal acidification defect was combined, which was demonstrated by increased fractional excretion of bicarbonate. 2) In mal control, intense H^+ -ATPase and band-3 protein staining was observed in collecting ducts. 3) In distal RTA patients, H6+ -ATPase and band-3 protein staining was not demonstrable or markedly decreased in the intercalated cells of distal nephron. 4) In two patients who had both proximal and distal RTA, H^+ -ATPase staining was markedly decreased in the brush border of proximal tubules as well as the distal nephron. In conclusion, the defect of acid-base transporters in renal tubule was related with the functional defect of urinary acidification in distal RTA.

품종별 무화과의 영양성분 비교

나환식 ( Hwan Sik Na ),김진영 ( Jin Young Kim ),박학재 ( Hak Jae Park ),최경철 ( Gyeong Cheol Choi ),양수인 ( Soo In Yang ),이지헌 ( Ji Heon Lee ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2013 한국식품저장유통학회지 Vol.20 No.3

국내에서 주로 재배되는 무화과 품종인 도후인(Dauphine)2종, 봉래시(Horaish)2종, 바나네(Banane) 1종을 대상으로 이화학적 특성을 분석한 결과, 무화과의 수분은 85.37~87.28%, 회분 0.38~0.43%, 조지방 0.20~0.26%, 조단백질 0.39~0.81%로 품종별 차이는 보이지 않았으며 조섬유는 바나네가 7.55±0.13%로 다른 품종 5.12~7.60%에 비해 다소 높은 결과를 보였다. 무기성분 함량은 모든 품종에서 K>Ca>Mg>Na순으로 나타났으며 전체 함량의 경우 도후인 품종이 타 품종에 비해 조금 높은 결과를 보였다. 바나네 품종과 봉래시 품종의 총 유리아미노산 조성은 glutamic acid>proline>aspartic acid 순으로 높은 분포를 보였으며, 전체 함량의 51.68~58.71%를 차지하고 있으며, 도후인 품종의 경우 glutamic acid>aspartic acid>alanine이 전체의 약 50%를 차지하고 있었다. 또한 기억력 감퇴 예방 등 학습 능력에 도움을 주는 기능성 물질인 γ-aminobutyric acid(GABA) 함량은 바나네에서 26.69mg/100g으로 상당히 높은 함량을 보였다. 총 폴리페놀 함량은 도후인이 198.91~261.64mg/kg, 봉래시 품종이 169.90~174.33mg/kg, 바나네 품종이 211.07mg/kg으로 나타났으며 총 플라보노이드 함량은 바나네(84.95mg/kg), 도후인(60.47~68.71mg/kg), 봉래시(44.12~44.60mg/kg)순으로 바나네가 가장 높았으며 품종간의 차이를 보였따. 플라보노이드성분 중 quercetin과 kaempferol은 각각 2.40~3.54mg/kg, 1.09~1.16mg/kg으로 분석되었다. In this study, the proximate compositions, minerals, free amino acids, total polyphenol and flavonoids of figs were analyzed according to their cultivars. The proximate compositions showed moisture at 85.37~87.28%, crude ash at 0.38~0.43%, crude lipid at 0.20~0.26%, crude protein at 0.39~0.81%, carbohydrate at 11.23~13.66% and crude fiber at 5.12~7.55% (dry base). The amounts of the following minerals in the figs were highest, in this order: K > Ca > Mg > Na. The total polyphenol contents were highest in this order: Dauphine (198.91~261.64mg/kg), Banane (211.07 mg/kg) and Horaish (169.90~174.33 mg/kg). The total flavonoid contents were highest in this order. Banane (84.52 mg/kg) > Dauphine (60.47~68.71 mg/kg) > Horaish (44.12~44.60mg/kg). The quercetin contents were highest in this order. Dauphine, 2.40~3.54 mg/kg; Banane, 3.54 mg/kg; and Horaish, 2.40~2.75 mg/kg; but the flavonoid contents were lowest in this order. Dauphine, 1.11~1.16 mg/kg; Banane, 1.14 mg/kg; and Horaish, 1.09~1.11 mg/kg. The free amino acid content of the figs was 199.70~328.77 mg/100g; their essential free amino acid contents, 46.45~67.46 mg/100 g; and their GABA (γ-aminobutyric acid) contents, 13.57~26.69 mg/100 g.

Hierarchically nanoporous pyropolymer nanofibers for surface-induced sodium-ion storage

Yoon, Hyeon Ji,Lee, Min Eui,Kim, Na Rae,Yang, Seung Jae,Jin, Hyoung-Joon,Yun, Young Soo Pergamon Press 2017 Electrochimica Acta Vol. No.

<P><B>Abstract</B></P> <P>Surface-driven charge storage materials based on both electrochemical double layer (EDL) formation and pseudocapacitive behavior can deliver high energy and power capabilities with long-lasting cycling performance. On the other hand, the electrochemical performance is strongly dependent on the material properties, requiring sophisticated electrode design with a high active surface area and a large number of redox-active sites. In this study, hierarchically nanoporous pyropolymer nanofibers (HN-PNFs) were fabricated from electrospun polyacrylonitrile nanofibers by simple heating with KOH. The HN-PNFs have a hierarchically nanoporous structure and an exceptionally high specific surface area of 3,950.7m<SUP>2</SUP> g<SUP>−1</SUP> as well as numerous redox-active heteroatoms (C/O and C/N ratio of 10.6 and 16.8, respectively). These unique material properties of HN-PNFs resulted in high reversible Na-ion capacity of ∼292mAhg<SUP>−1</SUP> as well as rapid kinetics and stable cycling performance in the cathodic potential range (1-4.5V vs. Na<SUP>+</SUP>/Na). Furthermore, energy storage devices based on HN-PNFs showed a remarkably high specific energy of ∼258 Wh kg<SUP>−1</SUP> at ∼245Wkg<SUP>−1</SUP> as well as a high specific power of ∼21,500Wkg<SUP>−1</SUP> at ∼78 Wh kg<SUP>−1</SUP>, with long and stable cycling behaviors over 2,000 cycles.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Hierarchically nanoporous pyropolymer nanofibers (HN-PNFs) were fabricated. </LI> <LI> HN-PNFs have a high specific surface area of ∼3,950m<SUP>2</SUP> g<SUP>−1</SUP> and numerous heteroatoms. </LI> <LI> HN-PNFs exhibit high capacities of ∼292mAhg<SUP>−1</SUP> and great rate/cycling performances. </LI> <LI> High energy (258Whkg<SUP>−1</SUP>) and high power (21,500Wkg<SUP>−1</SUP>) are achieved. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Hierarchically nanoporous pyropolymer nanofibers (HN-PNFs) fabricated from electrospun polyacrylonitrile nanofibers by simple heating with KOH exhibited high electrochemical performance for Na-ion storage.</P> <P>[DISPLAY OMISSION]</P>

每日 學習日誌 作成이 學習障碍兒의 學習戰略 使用, 動機的 信念, 그리고 學業成就에 미치는 效果

羅東晋,李在松,張澤洙 全北大學校 敎育大學院 1994 敎育論叢 Vol.14 No.-

Recent laboratory researchers have developed training programs and models of instruction to improve students' thinking skills in real-world instruction. These studies reflect the information-processing approaches to human thinking skills that focus on the differences between skilled and learning disabled learners in information-processing and have a great influence on the educational effort to diagnose and resolve the cognitive and motivational deficits of learning disabled learners. Based on these expectations, this study was attempted to find that the cognitive and motivational deficits of learning disabled learner could be remedied by keeping a Daily Learning Log. With this purpose, three specific hypotheses were formulated as follows: 1. The experimental group that keep a daily learning log would exhibit greater use in learning strategies(cognitive strategies and self-regulation) in the MSLQ test than would the control group that was given traditional instruction. 2. The experimental group that keep a daily learning log would exhibit enhancing motivational beliefs(self-efficacy and intrinsic motivation) than would the control group. 3. The experimental group that keep a daily learning log would exhibit greater academic performance in the three task test(social inquiry language foreign language)than would the control group. To solve these problems, an experimental treatment was carried out for about 4 months. Subjects were 32 first graders of a high school in a rural area of Chon-Buk. During 16 weeks, subjects in the experimental group studied 2 hours a week the learning strategies, motivation beleifs and keeping a daily learning log. The findings of this study were summarized as follows: 1. The expremental group exhibited greater use in learning strategies and self-regulation than did the control group, F(1, 29)=32. 19, p<0.001, F(1, 29)=47. 04, p<0.001. Accordingly, the Hypothesis 1 was accepted. 2. The experimental group that keep a daily learning log resulted in statistically significant enhancer motivational beliefs in self-eefficacy and intrinsic value than did the control group, F(1, 29)=52.14, p<0.001, F(1, 29)=14.36, p<0.001, consequently 2 accepted. 3. The experimental group that keep a daily learning log exhibited stattistically significant greater academic performance in two task(social inquiry domain, language domain) of the three task test(social inquiry domain, language domain, foreign language domain) than did the control group, F(1, 29)=36.21, p<0.001, F(1, 29)=22.96, P<0001, Accordingly, the hypothesis 3 was partially accepted. These results might suggest that the cognitive and motivational deficits of learning disabled learners could be modified by keeping a daily learning log.

연령증가에 따른 혈압요소들의 좌심실비대 및 관동맥질환과의 관련성

박재석,박창규,박미영,박재형,김용현,나진오,신성희,서순용,홍순준,박성미,임홍의,김응주,서홍석,오동주,노영무 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

혈액 및 비장조직에서 일산화탄소 헤모글로빈 농도에 관한 연구 : A Comparison of CO-Hb in Blood and Spleen Specimens

이진훈,오세진,김유나,이진숙,유재훈,장성길 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

성장기 어린이에서 상악 중절치 조기 상실 후 치조골 소실

나혜진,송제선,이제호,최형준,김성오,손흥규,최병재 大韓小兒齒科學會 2012 大韓小兒齒科學會誌 Vol.39 No.1

어린이는 8-10세 경에 외상을 자주 받으며 구강 내에서는 상악 절치부가 호발하는 부위이다. 외상으로 인하여 상악 절치가 상실되면 어린이에게 합병증을 야기할 수 있으므로 가능한 한 상악 절치를 유지해야 하지만 조기 상실되는 경우가 있다. 상악 절치의 조기 상실로 인한 합병증으로는 심미적 문제나 치조골의 수직적 높이와 수평적 너비의 감소, 인접치의 경사, 악궁 장 경 감소 등이 있다. 그 중에서 치조골이 소실되면 보철적 수복 시에 기능성과 안정성, 심미적인 면에 영향을 준다. 이 증례는 외상으로 인하여 상악 중절치가 조기 상실된 9세 여아와 6세 남아에서 각각 5년 5개월, 3년 7개월 후의 치조골 소실 정도를 cone beam computed tomography와 진단 모형 상에서 관찰하였기에 보고하는 바이다. The anterior maxillary incisor is the most traumatized region in the mouth and trauma is frequent between the ages of 8-10. Traumatic loss of teeth, can lead to many complications in children. Thus, as possible to keep traumatic teeth, but if you need extractions There may be. Complications occur and early tooth loss is frequent. Complications of early loss of central incisors are esthetic compromise, loss of vertical and horizontal width, height, contour of alveolar bone, tilting of adjacent teeth, arch length loss. Alveolar bone loss may affect normal function and stability, and results in esthetic problem for future prosthesis restoration. The 9-year-old girl and 6-year-old boy got early loss of upper central incisor. The amount of alveolar bone resorption was measured using cone beam computed tomograph and cast analysis.

IGCC용 고온건식 탈화제의 반응속도 모사에 관한 연구

박소진,이영우,민세홍,나재익,위영호 忠南大學校 産業技術硏究所 1999 산업기술연구논문집 Vol.14 No.1

고온건식 탈황공정에서 탈황제의 황화, 재생 반응은 비촉매 기고 반응이다. 이러한 비촉매 기고 반응의 수학적 모사는 실험데이터의 설명, 공정의 설계 그리고 scale-up에 있어서 매우 중요한 정보가 된다. 탈황제의 황화, 재생 반응에는 spray dryer로 제조된 zinc titanate 탈황제를 사용하였고, 변형된 미반응핵 모델식에 적용하여 각 반응의 반응속도를 해석한 결과 탈황제의 황화반응에는 화학반응 저항과 고체 상태의 확산 저항이 회합하여 전체반응속도를 지배하며, 이때 반응속도상수는 367.67cm/min 이었고 고체상태의 확산계수는 29.66cm²/min이었다. 또한 재생반응시에 는 화학반응 저항만이 전체반응의 저항으로 관여하며, 이때의 반응속도상수는 3.45x10e cm/min이었다. In the dry-type high temprature gas desulfurization process, sulfidation and regeneration reactions of desulfrrization sorbents are noncatalytic gas-solid reactions. The mathematical modeling of noncatalytic gas-solid reactions is improtant in order to interpret laboratory data and in design and scale-up. Zinc titanate sorbent to manufacture by spray dryer is used in sulfidation and regeneration reactions of desulfuization sorbents. The result which analyzes reaction rate of sulfidation reaction by applying each reactions to modified unreacted core model is that the global reaction rate is controlled by associating with chemical reaction resistance and solid state diffusion resistance. In this case,reaction rate constant is367.67e cm/min and solid state diffusion coefficient is 29.66e cm²/min Also gloval reaction rate of regeneration reaction is controlled only by chemical reaction resistance. In this case, reaction rate constant is 3.45x10e cm/min.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.