대하 정소의 cDNA 라이브러리 제작

서지선,김재만 木浦大學校 基礎科學硏究所 2003 基礎科學硏究誌 Vol.21 No.-

In the shrimp industry, female shrimps are preferred to males because the female grow faster and 1.5 to 2 times larger than male. The female, however, is not distinguished from male in appearance before it grow up to adult stage. This discourages the cultivators for the final yield drops by the biased male selection. In order to meet this problem, we tried to develop genetic diagnosis technique. Poly A mRNA was isolated from male testes to synthesize testis cDNA. The sum total 172㎍ of whole RNA was obtained from frozen teses in liquid nitrogen. By using biotin-labeled oligo (dT)_(20) and streptavidin-coated magnetic particles, 25㎕ of poly A^(+) RNA (573.8㎍/㎖) was yielded. Vector-primer was made to have Not I oligo (dT)_(25) primer at one end and it was hybridized to poly A^(+) RNA to construct cDNA by using reverse transcriptase. Synthesis of cDNA was confirmed by E. coli transformation and Southern hybridization.

상아질과 복합레진 접착부의 피로파괴에 관한 실험적 연구

이지연,김재곤,차경,백병주 大韓小兒齒科學會 1995 大韓小兒齒科學會誌 Vol.22 No.2

The purpose of this study was to investigated and evaluate the tensile bond strengths and fatigue fracture characteristics of four dentin adhesive systems to dentin surface. 99 extracted human teeth were randomly assigned to four groups, and exposed dentin surfaces treated with four dentin bonding agents. Resin composite was bonded to dentin by use of commercial adhesive systems. Thensile bond strengths were determined in an Instron universal testing machine at a crosshead speed of 1mm/min. and the fatigue characteristics were measured by the fatigue characteristics were measured by the Fatigue testing machine(Electro-dynamic type). Fractured dentin surfaces were examined by SEM. The following results were obtained. 1. The tensile bond strengths were 21.45MPa in All-Bond 2 group, 18.67MPa in AElite group, and 18.59MPa in Scotchbond Multi-Purpose group were higher than 10.29MPa in Gluma group(P<0.001). 2. The fatigue life of All-Bond 2, AELite and Scotchbond Multi-Purpose group were singificantly higher than Gluma group when a 24.13MPa cyclic flexure load was applied(P<0.001). 3. The fatigue life of All-Bond 2, AElite and Scotchbond Multi-Purpose group were decreased when a 39.21 or 45.25MPa cyclic flexure load was applied, but these were not showed statistically different(P>0.05). 4. In All-Bond 2, AElite and Scotchbond Multi-Purpose group, the fatigue fracture of resin/dentin interface occured within a resin-infiltrated area, but in Gluma group, at near its interface with adhesive layer.

CARIES-METER을 이용한 치아우식 치료법의 기준에 관한 고찰

이지연,백병주,정흥주 大韓小兒齒科學會 1992 大韓小兒齒科學會誌 Vol.19 No.1

The purpose of this study was finding of criteria for exact diagnosis of inital caries and advanced caries lesions. 57 primary molar teeth & 43 permanent first molar teeth of 75 children that visited pedodontic department of Chonbuk National University hospital were used for experiment. We compared with radiogrophic examination and suitable clinical treatment according to the extent of dental caries by using Caries Meter L. The obtained results were as folloWs. 1. Concerning of th equality rate in the primary molars and permanent teeth, the case of comparing radiographic examination and clinical treatment method in the highest reat as 98.24% in the primary molars, whereas the case of comparing Caries Meter L and clinical treatment is the highest rate as 90.7% in the permanent teeth. 2. When comparing of the measurement in Caries Meter L and radiographic examination of primary molars, the concurrent rate on extent of dental caries was 100% in the enamel, 78% in the dentin and 50% in the pulp, the Pearson Correlation was r=0.864 and there were significant difference statistically as significant level α=0.01(P<0.01). 3. When comparing of the measurement in Caries Meter L and clinical treatment method of primary molars, the concurrent rate on extent of dental caries was 100% in the enamel, 80% in the dentin and 55.6% in the pulp, the Pearson Correlation was r=0.877 and there were significant difference statistically as significant level α=0.01(P<0.01). 4. When comparing of the radiographic examination and clinical treatment method of primary molars, the concurrent rate was high as 90% in the pulp, the Pearson Correlation was r=0.982, and there were significant difference statistically as significant level α=0.01(P<0.01). 5. When comparing of the measurement in Caries Meter L and radiographic examination of permanent teeth, the concurrent rate was 78% in the enamel, 66.7% in the dentin and 100% in the pulp, the Pearson Correlation was r=0.689 and there were significant difference statistically as significant level α=0.01(P<0.01). 6. When comparing of the measurement in Caries Meter L, and clinical treatment method in the permanent teeth, the concurrent rate was 95.2% in the enamel, 85.7% in the dentin and 50% in the pulp, the Pearson Correlation was r=0.930 and there were significant difference statistically as significant level α=0.01(P<0.01). 7. When comparing of the radiographic examination and clinical treatment method in the permanent teeth, the concurrent rate was 74.1% in the enamel, 57.1% in the dentin and 50% in the pulp, the Pearson Correlation was r=0.681 and there were significant difference statistically as significant level α=0.01(P<0.01).

CARIES-METER를 이용한 치아우식 치료법의 기준에 관한 고찰

이지연,정홍주,백병주 全北大學校 齒醫學硏究所 1991 전북치대논문집 Vol.9 No.1

The purpose of this study was finding of criteria for exact diagnosis of initial caries and advanced caries lesions. 57 primary molar teeth & 43 permanent first molar teeth of 75 children that visited pedodontic department of chonbuk National University hospital were used for experiment. We compared with radiographic examination and suitable clinical treatment according to the extent of dental caries by using Caries Meter L. The obtained results were as follows. l. Concerning of the equality rate in the primary molars and permanent teeth, the case of comparing radiographic examination and clinical treatment method in the highest rate as 98.24% in the primary molars, whereas the case of comparing Caries Meter L and clinical treatment is the highest rate as 90.7% in the permanent teeth. 2. When comparing of the measurement in Caries Meter L and radiographic examination of primary molars, the concurrent rate on extent of dental caries was 100% in the enamel, 78% in the dentin and 50% in the pulp, the Pearson Correlation was r=0.864 and there were significant difference statistically as significant level α=0.01(P<0.01). 3. When comparing of the measurement in Caries Meter L and clinical treatment method of primary molars, the concurrent rate on extent of dental caries was 100% in the enamel, 80% in the dentin and 55.6% in the pulp, the Pearson Correlation was r=0.877 and there were significant difference statistically as significant level α= 0.01(P<0.01). 4. When comparing of the radiographic examination and clinical treatment method of primary molars, the concurrent rate was high as 90% in the pulp, the Pearson Correlation was r=0.982 and there were significant difference statistically as significant level α= 0.01(P<0.01). 5. When comparing of the measurement in Caries Meter L and radiographic examination of permanent teeth, the concurrent rate was 78% in the enamel, 66.7% in the dentin and 100% in the pulp, the Pearson Correlation was r=0.689 and there were significant difference statistically as significant level α=0.01(P<0.01). 6. When comparing of the measurement in Caries Meter L and clinical treatment method in the permanent teeth, the concurrent rate was 95.2% in the enamel, 85.7% in the dentin and 50% in the pulp, the Pearson Correlation was r=0.930 and there were significant difference statistically as significant level α=0.01(P<0.01). 7. When comparing of the radiographic examination and clinical treatment method in the permanent teeth, the concurrent rate was 74.1% in the enamel, 57.1% in the dentin and 50% in the pulp, the Pearson Correlation was r=0.681 and there were significant difference statistically as significant level α= 0.01(P<0.01).

치원성 석회화 낭종

이영수,이지연,백병주 大韓小兒齒科學會 1992 大韓小兒齒科學會誌 Vol.19 No.2

The calcifying odontogenic cyst is considered to occupy a position between a cyst and an odontogenic tumor-having some characteristics of both. The calcifying odontogenic cyst is a slow-growing commpletely benign codintion. It occured with equal frequency in the maxilla and mandible, their review of cases showed that 47% occured before 31 years of ages, and that 75% of the lesions were situated anterior to the first molar teeth. Most of the lesions are intrabony, but some occur in the soft tissue and may cause a saucering of the adjacent bone, unerupted teeth and root resorption were observed in approximately one half of the cases. The radiographic appearance in of a cystlike radiolucency containing quite distinct radiopaque foci. The microscopic picture is unique for an oral lesion and the cells above the basal layer are irregular in arrangement and surround nests or sheets of large ghost epithelial cells filled with atypical-appearing keratin. Since the calcifying odontogenic cyst has a tendency for continued growth, surgical enucleation is the treatment of choice. Recurrence occur on occasion.

법랑질 형성 부전증에 대한 증례보고

이승영,백병주,이지연 大韓小兒齒科學會 1994 大韓小兒齒科學會誌 Vol.21 No.1

Amelogenesis imperfecta(AI) is defined as a genetically determined effect affecting enaml formation and may be associated with other ectodermal or systemic disorders. It is entriely an ectodermal disturbance, since the mesodermal components of the teeth are basically normal. The presentation of diverse clinical manifestations in thought to result from the heterogeneous structural and chemical defects. The anomaly occurs in the general population in the range of 1 n 14,000 to 16,000, to 1.4 in 1000, depending on the specific population studied. Classification of the AI types considers mode of inheritance and clinical manifestations. The most widely accepted classifi-cation system recognizes three major groups ; i.e., hypoplastic(thin enamel), hypocalcified (primary mineralization defect), hypomaturation(defect in enamel maturation). Delineationg speific AL types can be confusing given the phenotypical similarity of many forms and that the most recent classification lists 14 different AI types. A 8-years-old female patient with complaint of abnoraml crown shape and discoloration of maxillary anterior tooth and excessice wear of posterior dentition of both arch.came to Dept.of pedodontics, College of Dentistry, Chonbut National University for rahabilitation. The treatment depends on its severity and the demands of esthetic improvement. In this case, resin veneer crown of maxillary anteiror tooth and composite resin restoration of mandibular anterior tooth showed good esthetic result. Restoration of Mx. and Mn. 1st first molar with stainless steel crown rehabilitated the masticatory function and estabilished the vertical dimension of occlusion. But futher care is needed for estabilishment of final vertical dimension of occlusion after completion of permanent dentition.

Pendulum 장치를 이용한 상악 대구치 후방이동에 관한 치험례

김문현,백병주,이지연 大韓小兒齒科學會 1993 大韓小兒齒科學會誌 Vol.20 No.2

Orthodontists have long sought methods of correcting ClassⅡ malocclusions without straining the lower arch and without the need for strict patient compliance. In the 1990s, noncompliance therapies in various forms have become more prominent than ever before. The Pendulum Appliance is a hybrid that uses a large Nance acrylic button in the palate for anchorage, along with 032˝TMA springs that deliver a light, continuous force to the upper first molars without affecting the palatal button. Thus, the appliance produces a broad, swinging arc-or pendulum- of force from the midine of the palate to the upper molars. Since the Pendulum Appliance drives the upper molars distally (with slight lingual tipping) quite rapidly, there is a tendency for the anterior bite to open. This open bite generally corrects itself in brachyfacial types, especially those with tongue-thrust habits. I still recommend treating vertical growth patterns conservatively with extraction, directional headgears, and transpalatal bars. Patient tolerance of the Pendulum Appliance has been excellent; most adapt to the appliance within a week, Initial clinical findings have been encouraging. It is not unusual to see as much as 5mm of distal molar movement in three to four months.

동시형광 분광광도법에 의한 대기 시료 중의 다환방향족 탄화수소(PAHs)의 분석

유광식,정선이,정지영 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.1

Determination of some PAHs in ambient air at Ulsan have been carried out by collection of the components into n-hexane followed by synchronous spectrofluorimetric technique. 10 PAHs, such as acenaphthene (Ace), anthracene (Anth), benz[a]anthracene (BaA), benzo[b]fluoranthene (BbFt), benzo[k]fluoranthene (BkFt) benzo [a]pyrene (BaP), chrysene (Chry), phenanthrene (Phen), fluoranthene (Ft), perlyrene (Per), and pyrene (Pyr) in air samples were able to determine separately by synchronous spectrofluorimetry. Calibration curves for those components were linear for the concentration range of 0.2∼166 ppb PAHs with the correlation factor of 0.9985∼0.9999. The predominant contribution was phenanthrene which was included 36.9∼85.1% to the overall level of the 10 PAHs in some areas, Also benzo[a]pyrene which was known to carcinogenicity was detected from 6.4 to 55.8 ng/㎥, benzo[a]anthracene of some areas was contained from 21.9∼153 ng/㎥,

발암성물질에 대한 어린이의 장기적 노출 및 위해성 평가

양지연(JY Yang),임영욱(YK Lim),김호현(HH Kim),이용진(YJ Lee),김진용(JY Kim),신동천(DC Shin) 환경독성보건학회 2012 한국독성학회 심포지움 및 학술발표회 Vol.2012 No.10

블루투스 비콘을 활용한 의료현장에서의 실내 위치 기반 모니터링 시스템에 대한 연구

조재윤(JY Jo),육진영(Jy Youk),이선재(SJ Lee),장혁재(Hj Chang),송시영(Sy Song),한태화(TH Han) 대한전자공학회 2019 대한전자공학회 학술대회 Vol.2019 No.11