면양(緬羊)에 의한 Formaldehyde 처리 Alfalfa 엽(葉) 분미의 단백질 가치평가에 관한 연구

강희신,R. H. Weston,J. R. Ashes,P. Davis,R. W. Edols 한국낙농학회 1985 韓國酪農學會誌 Vol.7 No.4

低質 粗飼科 밀짚으로 飼育되는 交雜種 緬羊 6頭를 供試하며 alfalfa 葉 粉末, 當年葉, 貯藏葉, 2% HCHO處理 當年葉 및 4% HCHO處理貯藏葉을 供試 飼料로 하고 밀짚 基本飼料 700g 및 alfalfa 粉末 補充飼料 300g 計 1.0㎏를 日量 飼料로 連續 給餌器에서 3시간 間隔으로 1日 8回 給與하며 20日間씩의 代謝試驗을 4回 實施하였다. ^(51)Cr-EDTA 및 ^(103)Ru-phe의 二重 標識物質을 使用하여 腸內 內容物의 通過量 및 滯在時間反芻胃 溶量, 有機物 및 窒素의 利用性을 測定한 結果 다음과 같은 結果를 얻었다. 1. 反芻胃 溶液中의 NH₃態 窒素 濃度는 貯藏葉이 當年葉보다 17.5% 높으며(P<.01) 2% HCHO處理 當年葉은 그 無處理보다 7% 程度 減少되고 4% HCHO處理 貯藏葉은 無處理보다 34.0% 程度 減少(P<.01)되었다. 2. 反芻胃로부터 流入되는 NH₃態 窒素 量은 當年葉보다 貯藏葉이 18% 增加(P<.01)되고 2% HCHO處理 當年葉은 無處理보다 6% 減少 (P<.05)되며, 4% HCHO處理 貯藏葉은 無處理보다 32% 減少(P<.01)를 나타내었다. 3. 第4胃內 NAN含量은 當年葉이 貯藏葉보다 6% 增加되며 2% HCHO處理 當年葉은 無處理보다 5.6%의 增加(P<.05) 4% HCHO處理 貯藏葉은 그 無處理보다 17% 程度의 增加(P<.01)를 나타내었다. 4. 第4胃로부터 排出되어 小腸에 流入되는 NAN의 量은 當年葉보다 貯藏葉이 9.0% 더 流入되며(P<.05) 2% HCHO 處理 當年葉은 그 無處理보다 6% 程度 增加(P<.05) 流人되었다. 5. 飼料源 室素 100g 攝取量當 NAN의 小腸內 消化量은 當年葉보다 貯藏葉이 10% 程度 消化가 增進(P<.05)되며 2% HCHO 處理 當年葉은 無處理보다 6% 程度 消化가 增進(P<.05)되었다. 6. 飼料源 室素 100g 攝取量 當 糞中 窒素의 排泄量은 當年葉보다 貯藏葉이 8% 정도(P<.01), 2% HCHO處理 當年葉은 無處理보다 6% 程度(P<.05) 더 排泄되었으며 4% HCHO處理葉은 그 無處理에 比하여 2% 程度 더 排泄되었으나 有意差는 認定되지 않았다. 7. 反芻胃液(Y, ㎎ N%)과 第4胃 濾液(X, ㎎ N%)中의 NH₃態 室素 濃度와의 關係는 다음 回歸 方程式으로 表示되었다. Y = 3.981 + 1.2783(±0.3736)X (r = 0.59, n-2=22) 8. 飼料 窒素 攝取量에 對한 補充飼料 alfalfa 葉 粉末의 小腸內 流入 NAN의 百分比는 當年葉, 2% HCHO處理 當年葉 및 無處理 貯藏葉의 順으로 각각 57.0%, 68.0% 및 65%로 推定되었다. 따라서 小腸內 流入 NAN은 2% HCHO處理 當年葉이 無處理보다 19%, 貯藏葉은 當年葉 無處理보다 14% 程度 增加된 것으로 推定된다. 9. 貯藏葉 補充時 音機物 消化率은 當年葉을 補充할 때 보다 約 1% 減少되고 當年葉 및 貯藏葉에 HCHO處理는 葉中 有機物의 排泄量을 0∼3.4% 程度 增加시키는 傾向이었으나 有意差는 當年葉과 貯藏葉 間에만 認定되었다. 10. 反芻胃液 및 第4胃液의 各 腸器 通過率, 反芻胃 容量 및 그 內容物의 滯在時間은 處理間有意差가 認定되지 않았다. 1. The OM out-put in the faeces was about 3% unit higher with the Old than that with the New, while with the HCHO treated meals there was only a slightly and insignificantly increasing tendency in the OM out-put. 2. No significant differences in the liquor flow rate of the rumen and the abomasum fluid, and in the rumen volume and retention time were found between the treatments. 3. The ruminal NH₃-N concentration in the Old was about 17.5% unit higher (P<0.01) than that in the New, while the New + 2% HCHO was about 7.0% unit (P<0.10) and the Old + 4% HCHO was about 34% lower (P<0.01) than those in the untreated-New and Old. 4. The amount of NH₃-N excreted from the rumen in the Old was 18% unit higher (P<0.01) than that in the New, and that in the New + 2% HCHO about 6% unit was lower, though insignificant, than that in the New, while that in the Old + 4% HCHO was about 32% unit lower (P<0.01) than that in the Old. 5. The NAN content of the intestine in the New was about 6% unit higher (P<0.10) than that in the Old, while in the New + 2% HCHO and the Old + 4% HCHO about 6% unit (P<0.05) and 17% unit (P<0.01) were higher than those in the New and the Old, respectively. 6. The amount of NAN excreted from the abomasum in the Old was about 9% unit higher (P<0.05) than that in the New, while the New + 2% HCHO resulted about 6% unit higher (P<0.01) NAN excretion than the New. 7. The NAN digested in the intestine per 100g of dietary nitrogen intake in the Old was about 10% unit higher (P<0.10) than that in the New, while in the New + 2% HCHO about 6% unit was higher (P<0.05) than that in the New. 8. The fecal nitrogen output per 100g of dietary nitrogen intake in the Old was about 8% unit (P<0.01), in the New + 2% HCHO was about 6% unit and in the Old + 4% HCHO about 2.4% unit was higher (P<0.10) than in the New and the Old. 9. The significant correlation between the concentrations of NH₃-N in the abomasal filtrates and those in the ruminal fluids permitted to draw a predictive equation by regression analysis as follows: y = 3.981 + 1.2783(+0.3736) X, where, Y = Ruminal NH₃-N concentration (㎎ N%) X= NH₃-N concentration in abomasal filtrates (㎎ N%), (n-2=22) and (r=0.59) 10. The percent of NAN entered the intestines over supplemental leaf meal nitrogen intake in the New, the New + 2% HGHO and the Old were 57.0, 68.0 and 65%, respectively. The NAN entered the intestine in the New + 2% HCHO and the Old were 19% and 14% higher than those in the New, respectively.

Clinical efficacy and safety of abatacept in methotrexate-naive patients with early rheumatoid arthritis and poor prognostic factors

Westhovens, R,Robles, M,Ximenes, A C,Nayiager, S,Wollenhaupt, J,Durez, P,Gomez-Reino, J,Grassi, W,Haraoui, B,Shergy, W,Park, S-H,Genant, H,Peterfy, C,Becker, J-C,Covucci, A,Helfrick, R,Bathon, J BMJ Group 2009 Annals of the Rheumatic Diseases Vol.68 No.12

<P><B>Objectives:</B></P><P>To assess the efficacy and safety of abatacept in methotrexate-naive patients with early rheumatoid arthritis (RA) and poor prognostic factors.</P><P><B>Methods:</B></P><P>In this double-blind, phase IIIb study, patients with RA for 2 years or less were randomly assigned 1 : 1 to receive abatacept (∼10 mg/kg) plus methotrexate, or placebo plus methotrexate. Patients were methotrexate-naive and seropositive for rheumatoid factor (RF), anti-cyclic citrullinated protein (CCP) type 2 or both and had radiographic evidence of joint erosions. The co-primary endpoints were the proportion of patients achieving disease activity score in 28 joints (DAS28)-defined remission (C-reactive protein) and joint damage progression (Genant-modified Sharp total score; TS) at year 1. Safety was monitored throughout.</P><P><B>Results:</B></P><P>At baseline, patients had a mean DAS28 of 6.3, a mean TS of 7.1 and mean disease duration of 6.5 months; 96.5% and 89.0% of patients were RF or anti-CCP2 seropositive, respectively. At year 1, a significantly greater proportion of abatacept plus methotrexate-treated patients achieved remission (41.4% vs 23.3%; p<0.001) and there was significantly less radiographic progression (mean change in TS 0.63 vs 1.06; p = 0.040) versus methotrexate alone. Over 1 year, the frequency of adverse events (84.8% vs 83.4%), serious adverse events (7.8% vs 7.9%), serious infections (2.0% vs 2.0%), autoimmune disorders (2.3% vs 2.0%) and malignancies (0.4% vs 0%) was comparable for abatacept plus methotrexate versus methotrexate alone.</P><P><B>Conclusions:</B></P><P>In a methotrexate-naive population with early RA and poor prognostic factors, the combination of abatacept and methotrexate provided significantly better clinical and radiographic efficacy compared with methotrexate alone and had a comparable, favourable safety profile.</P>

<i>In vitro</i> inhibitory effects of Wen‐pi‐tang‐Hab‐Wu‐ling‐san on human cytochrome P450 isoforms

Lee, H. W.,Kim, D. W.,Phapale, P. B.,Lim, M. ‐,S.,Park, J.,Seo, J. J.,Park, K. M.,Park, Y. ‐,K.,Yoon, Y. ‐,R. Blackwell Publishing Ltd 2011 Journal of clinical pharmacy and therapeutics Vol.36 No.4

<P><B>Summary</B></P><P><B>What is known and Objective: </B> Although Wen‐pi‐tang‐Hab‐Wu‐ling‐san (WHW), an oriental herbal medicine, has been prescribed for the treatment of chronic renal failure (CRF) in Korean clinics, no studies regarding WHW–drug interactions had been reported. The purpose of this study was to evaluate the possibility that WHW inhibits the catalytic activities of major cytochrome P450 (CYP) isoforms.</P><P><B>Methods: </B> The abilities of various WHW extracts to inhibit phenacetin O‐de‐ethylation (CYP1A2), tolbutamide 4‐methylhydroxylation (CYP2C9), omeprazole 4′‐hydroxylation (CYP2C19), dextromethorphan O‐demethylation (CYP2D6), chlorzoxazone 6‐hydroxylation (CYP2E1) and midazolam 1‐hydroxylation (CYP3A4) were assessed using human liver microsomes.</P><P><B>Results and Discussion: </B> WHW extract at concentrations up to 100 μ<SMALL>m</SMALL> showed negligible inhibition of the six CYP isoforms tested (CYP1A2, CYP2C9, CYP2C19, CYP2D6, CYP2E1 and CYP3A4), with apparent IC<SUB>50</SUB> values (concentration of the inhibitor causing 50% inhibition of the original enzyme activity) of 817.5, 601.6, 521.7, 310.2, 342.8 and 487.0 μg/mL, respectively.</P><P><B>What is new and Conclusion: </B> Our <I>in vitro</I> findings suggest that WHW extract at concentrations corresponding to a clinically recommended dosage range has no notable inhibitory effects on CYP isoforms. Therefore, we believe that WHW extract may be free of drug–herb interactions when co‐administered with other medicines. However, <I>in vivo</I> human studies are needed to confirm these results.</P>

Search for Λc+→ϕpπ0 and branching fraction measurement of Λc+→K−π+pπ0

Pal, B.,Schwartz, A. J.,Adachi, I.,Aihara, H.,Al Said, S.,Asner, D. M.,Aushev, T.,Ayad, R.,Badhrees, I.,Bakich, A. M.,Bansal, V.,Behera, P.,Berger, M.,Bhardwaj, V.,Biswal, J.,Bobrov, A.,Bozek, A.,Bra& American Physical Society 2017 Physical review. D Vol.96 No.5

<P>We have searched for the Cabibbo-suppressed decay Lambda(+)(c) -> pi p(0) in e(+) e(-) collisions using a data sample corresponding to an integrated luminosity of 915 fb(-1). The data were collected by the Belle experiment at the KEKB e(+) e(-) asymmetric-energy collider running at or near the (4S) and (5S) resonances. No significant signal is observed, and we set an upper limit on the branching fraction of B(Lambda(+)(c) -> phi p(0)) < 15.3 x 10(-5) at 90% confidence level. The contribution of nonresonant Lambda(+)(c) -> K+ K- p pi(0) decays is found to be consistent with zero, and the corresponding upper limit on its branching fraction is set to be B(Lambda(+)(c) ->. K+ K- p pi(0))(NR) < 6.3 x 10(-5) at 90% confidence level. We also search for an intermediate hidden-strangeness pentaquark decay P-s(+) -> phi p. We see no evidence for this intermediate decay and set an upper limit on the product branching fraction of B(Lambda(+)(c) -> P-s(+) pi(0)) x B(P-s(+) -> phi p) < 8.3 x 10(-5) at 90% confidence level. Finally, we measure the branching fraction for the Cabibbo-favored decay Lambda(+)(c) -> K- pi(+) p pi(0); the result is B(Lambda(+)(c) -> K- pi(+) p pi(0)) = (4.42 +/- 0.05(stat)+/- 0.12(syst)+/- 0.16(norm))%, which is the most precise measurement to date.</P>

Expression phenotype changes of EBV-transformed lymphoblastoid cell lines during long-term subculture and its clinical significance

Lee, J.-E.,Nam, H.-Y.,Shim, S.-M.,Bae, G.-R.,Han, B.-G.,Jeon, J.-P. Blackwell Publishing Ltd 2010 Cell proliferation Vol.43 No.4

<P>Abstract</P><P>Objectives: </P><P>The EBV-transformed lymphoblastoid cell line (LCL) is a useful resource for population-based human genetic and pharmacogenetic studies. The principal objective here was to assess expression phenotype changes during long-term subculture of LCLs, and its clinical significance.</P><P>Materials and methods: </P><P>We searched for genes that were differentially expressed in 17 LCLs at late (p161) passage compared to early passage (p4) using microarray assay, then validated them by real-time RT-PCR analysis. In addition, we estimated correlations between expression phenotypes of 20 LCL strains at early passage and 23 quantitative clinical traits from blood donors of particular LCL strains.</P><P>Results: </P><P>Transcript sequences of 16 genes including nuclear factor-&kgr;B (NF-&kgr;B) pathway-related genes (such as <I>PTPN13</I>, <I>HERC5</I> and miR-146a) and carcinogenesis-related genes (such as <I>XAF1</I>, <I>TCL1A</I>, <I>PTPN13</I>, <I>CD38</I> and miR-146a) were differentially expressed (>2-fold change) in at least 15 of the 17 LCL strains. In particular, <I>TC2N</I>, <I>FCRL5</I>, <I>CD180</I>, <I>CD38</I> and miR-146a were downregulated in all 17 of the evaluated LCL strains. In addition, we identified clinical trait-associated expression phenotypes in LCLs.</P><P>Conclusion: </P><P>Our results showed that LCLs acquired expression phenotype changes involving expression of NF-&kgr;B pathway- and carcinogenesis-related genes during long-term subculture. These differentially expressed genes can be considered to be a gene signature of LCL immortalization or EBV-induced carcinogenesis. Clinical trait-associated expression phenotypes should prove useful in the discovery of new candidate genes for particular traits.</P>

Paclitaxel suppresses Tau-mediated microtubule bundling in a concentration-dependent manner

Choi, Myung Chul,Chung, Peter J.,Song, Chaeyeon,Miller, Herbert P.,Kiris, E.,Li, Youli,Wilson, Leslie,Feinstein, Stuart C.,Safinya, Cyrus R. Elsevier 2017 Biochimica et biophysica acta, General subjects Vol.1861 No.1

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Microtubules (MTs) are protein nanotubes comprised of straight protofilaments (PFs), head to tail assemblies of αβ-tubulin heterodimers. Previously, it was shown that Tau, a microtubule-associated protein (MAP) localized to neuronal axons, regulates the average number of PFs in microtubules with increasing inner radius <<I> R</I> <SUB>in</SUB> <SUP>MT</SUP> > observed for increasing Tau/tubulin-dimer molar ratio Φ<SUB>Tau</SUB> at paclitaxel/tubulin-dimer molar ratio Λ<SUB>Ptxl</SUB> =1/1.</P> <P><B>Methods</B></P> <P>We report a synchrotron SAXS and TEM study of the phase behavior of microtubules as a function of varying concentrations of paclitaxel (1/32≤Λ<SUB>Ptxl</SUB> ≤1/4) and Tau (human isoform 3RS, 0≤Φ<SUB>3RS</SUB> ≤1/2) at room temperature.</P> <P><B>Results</B></P> <P>Tau and paclitaxel have opposing regulatory effects on microtubule bundling architectures and microtubule diameter. Surprisingly and in contrast to previous results at Λ<SUB>Ptxl</SUB> =1/1 where microtubule bundles are absent, in the lower paclitaxel concentration regime (Λ<SUB>Ptxl</SUB> ≤1/4), we observe both microtubule doublets and triplets with increasing Tau. Furthermore, increasing paclitaxel concentration (up to Λ<SUB>Ptxl</SUB> =1/1) slightly decreased the average microtubule diameter (by ~1 PF) while increasing Tau concentration (up to Φ<SUB>3RS</SUB> =1/2) significantly increased the diameter (by ~2–3 PFs).</P> <P><B>Conclusions</B></P> <P>The suppression of Tau-mediated microtubule bundling with increasing paclitaxel is consistent with paclitaxel seeding more, but shorter, microtubules by rapidly exhausting tubulin available for polymerization. Microtubule bundles require the aggregate Tau-Tau attractions along the microtubule length to overcome individual microtubule thermal energies disrupting bundles.</P> <P><B>General significance</B></P> <P>Investigating MAP-mediated interactions between microtubules (as it relates to <I>in vivo</I> behavior) requires the elimination or minimization of paclitaxel.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Increasing paclitaxel suppresses Tau-mediated microtubule bundling. </LI> <LI> A length-dependent mechanism for Tau-mediated microtubule bundling is proposed. </LI> <LI> Understanding MAP/microtubule behavior requires elimination of paclitaxel use. </LI> </UL> </P>

Disruption of a regulatory loop between DUSP1 and p53 contributes to hepatocellular carcinoma development and progression

Hao, P.P.,Li, H.,Lee, M.J.,Wang, Y.P.,Kim, J.H.,Yu, G.R.,Lee, S.Y.,Leem, S.H.,Jang, K.Y.,Kim, D.G. Elsevier Science Publishers 2015 Journal of hepatology Vol.62 No.6

Background & Aims: Altered expression of dual specificity phosphatase 1 (DUSP1) is common in tumors including hepatocellular carcinoma (HCC), and is predictive of tumor progression and poor prognosis. However, the tumor suppressive role of DUSP1 has yet to be clearly elucidated. Methods: The molecular mechanisms of tumor suppression that were investigated were induction of apoptosis, cell cycle inhibition, and regulation of p53. Additionally, the antitumor effect of DUSP1 was assessed using a mouse model. Associated signaling pathways in HCC cells and tissues were examined. Results: Downregulation of DUSP1 expression was significantly correlated with poor differentiation (p<0.001) and advanced HCC stage (p=0.023). DUSP1 expression resulted in HCC suppression and longer survival (p=0.0002) in a xenoplant mice model. DUSP1 inhibited p38 MAPK phosphorylation and subsequently suppressed HSP27 activation, resulting in enhanced p53 phosphorylation at sites S15, S20, and S46 in HCC cells. Enhanced p53 activation induced the expression of target genes p21 and p27, which are linked to cell cycle arrest and apoptosis. Thus, DUSP1 was potentially linked to p53 activation via the p38 MAPK/HSP27 pathway. Wild-type but not mutant p53 transcriptionally upregulated DUSP1 via its DNA-binding domain. DUSP1 and p53 might collaborate to suppress tumors in hepatocarcinogenesis via a positive regulatory loop. Conclusions: Our results revealed that disruption of a positive regulatory loop between DUSP1 and p53 promoted HCC development and progression, providing a rationale for a therapeutic agent that restores DUSP1 in HCC.

The multiple merger assembly of a hyperluminous obscured quasar at redshift 4.6

Dí,az-Santos, T.,Assef, R. J.,Blain, A. W.,Aravena, M.,Stern, D.,Tsai, C.-W.,Eisenhardt, P.,Wu, J.,Jun, H. D.,Dibert, K.,Inami, H.,Lansbury, G.,Leclercq, F. American Association for the Advancement of Scienc 2018 Science Vol.362 No.6418

<P><B>Mergers drive a powerful dusty quasar</B></P><P>Massive galaxies in the early Universe host supermassive black holes at their centers. When material falls toward the black hole, it releases energy and is observed as a quasar. Astronomers found a population of powerful distant quasars that are obscured by dust, but it has been unclear how they are formed. Díaz-Santos <I>et al.</I> observed the dust-obscured quasar WISE J224607.56-052634.9 at submillimeter wavelengths, finding three small companion galaxies connected to the quasar by bridges of gas and dust. They inferred that galaxy mergers can provide both the raw material to power a quasar and large quantities of dust to obscure it.</P><P><I>Science</I>, this issue p. 1034</P><P>Galaxy mergers and gas accretion from the cosmic web drove the growth of galaxies and their central black holes at early epochs. We report spectroscopic imaging of a multiple merger event in the most luminous known galaxy, WISE J224607.56−052634.9 (W2246−0526), a dust-obscured quasar at redshift 4.6, 1.3 billion years after the Big Bang. Far-infrared dust continuum observations show three galaxy companions around W2246−0526 with disturbed morphologies, connected by streams of dust likely produced by the dynamical interaction. The detection of tidal dusty bridges shows that W2246−0526 is accreting its neighbors, suggesting that merger activity may be a dominant mechanism through which the most luminous galaxies simultaneously obscure and feed their central supermassive black holes.</P>

Effect of night light regimen on growth performance, antioxidant status and health of broiler chickens from 1 to 21 days of age

R.X. Zhao,C.H. Cai,P. Wang,L. Zheng,J.S. Wang,K.X. Li,W. Liu,X.Y. Guo,X. A. Zhan,K.Y. Wang 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.6

Objective: The study was conducted to evaluate the effects of night light regimen on growth performance, antioxidant status and health of Lingnan Yellow broiler chickens from 1 to 21 days of age. Methods: A completely randomized factorial design involved 2 photoperiods (constant lighting [CL], 24 L:0 D and intermittent lighting [INL], 17 L:3 D:1 L:3 D)×2 light intensities (10 lx and 30 lx). A total of one thousand six hundred and eighty 1-d-old Lingnan Yellow broiler chicks were randomly divided into 4 treatments with 6 replicates (70 birds per replicate). The experiment lasted for 21 d. Results: Photoperiods and light intensities had no effect on average daily gain, feed conversion ratio, and mortality of the broiler chickens (p>0.05). The INL had a significant effect on average daily feed intake (p<0.05) of broiler chickens compared with CL. Photoperiod and light intensity had an interactive effect on melatonin (MT) concentration (p<0.05). At CL, reducing light intensity increased MT concentration; INL birds had higher MT but MT concentration was not affected by light intensity. There was an interactive effect on glutathione peroxidase (GPx) and catalase (CAT) in serum and total antioxidant capability (T-AOC) in liver between photoperiod and light intensity. With the decrease of light intensity, the activities of GPx and CAT in serum and T-AOC in liver increased in CL group (p<0.05). Broiler chickens reared under INL had better antioxidant status and 10 lx treatments had higher activities of CAT in serum than 30 lx (p<0.05). Different photoperiods and light intensities had no effect on malondialdehyde. There was an interaction between photoperiod and light intensity on serum creatine kinase (CK) concentration (p<0.05). At CL, the elevated light intensity resulted in an increase in CK content; INL birds had lower CK concentration especially in low light intensity group. Besides, INL and low light intensity significantly reduced the concentration of serum corticosterone and heat shock protein 70 (p<0.05). Serum immunoglobulin M contents were increased in broiler chickens reared under the INL compared with CL group (p<0.05). Conclusion: Results above suggest that the night light regimen of INL and 10 lx could be beneficial to the broiler chickens from 1 to 21 days of age due to the better health status and electricity savings.

THE 2014 ALMA LONG BASELINE CAMPAIGN: OBSERVATIONS OF THE STRONGLY LENSED SUBMILLIMETER GALAXY HATLAS J090311.6+003906 AT <i>z</i> = 3.042

Vlahakis, C.,Hunter, T. R.,Hodge, J. A.,Pé,rez, L. M.,Andreani, P.,Brogan, C. L.,Cox, P.,Martin, S.,Zwaan, M.,Matsushita, S.,Dent, W. R. F.,Impellizzeri, C. M. V.,Fomalont, E. B.,Asaki, Y.,Barka IOP Publishing 2015 ASTROPHYSICAL JOURNAL LETTERS - Vol.808 No.1

<P>We present initial results of very high resolution Atacama Large Millimeter/submillimeter Array (ALMA) observations of the z = 3.042 gravitationally lensed submillimeter galaxy HATLAS J090311.6+003906 (SDP. 81). These observations were carried out using a very extended configuration as part of Science Verification for the 2014 ALMA Long Baseline Campaign, with baselines of up to similar to 15 km. We present continuum imaging at 151, 236, and 290 GHz at unprecedented angular resolutions as fine as 23 mas, corresponding to an unmagnified spatial scale of similar to 180 pc at z = 3.042. The ALMA images clearly show two main gravitational arc components of an Einstein ring, with emission tracing a radius of similar to 1 ''.5. We also present imaging of CO J = 10 - 9, J = 8 - 7, and J = 5 - 4 and H2O (202-111) line emission. The CO emission, at an angular resolution of similar to 170 mas, is found to broadly trace the gravitational arc structures but with differing morphologies between the CO transitions and compared to the dust continuum. Our detection of H2O line emission, using only the shortest baselines, provides the most resolved detection to date of thermal H2O emission in an extragalactic source. The ALMA continuum and spectral line fluxes are consistent with previous Plateau de Bure Interferometer and Submillimeter Array observations despite the impressive increase in angular resolution. Finally, we detect weak unresolved continuum emission from a position that is spatially coincident with the center of the lens, with a spectral index that is consistent with emission from the core of the foreground lensing galaxy.</P>