소아의 연령별 Propionibacterium acnes에 대한 균체응집항체 및 동종혈구응집항체간의 비교연구

정화영,정상인,최철순,양용태 중앙대학교 의과대학 의과학연구소 1984 中央醫大誌 Vol.9 No.3

In general, the concentration of maternal IgG globulin in serum of the neonates falls rapidly within the first few months after birth and the production of IgM globulin develops in the maturing infants during the course of exposure to various antigens in the environment. The development of natural antibodies, ie., isohemagglutinins, IgM class, to ABO blood group substances and agglutinins, Ig class, to normal flora, in the early stage of life is important since not only they may act as bactericidal substances in nonspecific manners, but also could be immunological barometers on the normal function of humoral immune system. The high concentration of isohemagglutinins to human A or B blood group antigens and agglutinating antibodies to some of normal flora such as Propionibacterium acnes or Staphylococcus aureus were observed in normal human sera. It has been known that the serum concentration of IgM globulin usually reached adult levels by one year of age, while that of IgG globulins by five to six years of age. However, the levels of isohemagglutinins to A and B group substances and agglutinating to Propionibacterium acnes in children's sera and the ages in which the concentrations of their antibodies reached to abult levels are not clarified. In this study, the concentrations of isohemagglutinins to A and B blood group antigens and agglutinating antibodies to P.acnes serotype Ⅰ and serotype Ⅱ in the sera of 163 normal children, ranged from 0 day to 15 years of age, were measured by means of microtitration technique. The results obtained are as follows: 1. In the sera of 163 children under 15 years of age, there observed no significant difference in the titers of agglutinating antibodies to P. acnes serotype Ⅰ andⅡ. 2. Of 75 sera of children under one year of age, the numbers of sera in which agglutinating antibodies were not detectable or less than 1:4 to P. acnes serotype Ⅰ were 67(89.6%) and to serotype Ⅱ 53(70.7%), respectively. 3. Agglutinating antibody to P. acnes serotype Ⅰ in the children's sera reached adult levels by 7 years of age, but 100 percentages of antibody detection was observed only in the age group of 15 years old, whereas the adult levels of isohemagglutinins to A and B blood group antigens were observed in the age group of 6 months old. 4. No correlation were observed in normal children's sera between agglutinating antibody titre to P. acnes serotype Ⅰ and isohemagglutinin titre. These results indicated that the isohemagglutinins to ABO blood substances appeared in the earlier stage of life than did agglutinating antibodies to P. acnes.

<i>In vitro</i> and <i>in vivo</i> evaluation of a novel polymer-free everolimus-eluting stent by nitrogen-doped titanium dioxide film deposition

Park, Dae Sung,Bae, In-Ho,Jeong, Myung Ho,Lim, Kyung Seob,Sim, Doo Sun,Hong, Young Joon,Lee, So-Youn,Jang, Eun Jae,Shim, Jae-Won,Park, Jun-Kyu,Lim, Han Chul,Kim, Han Byul Elsevier 2018 Materials Science and Engineering C Vol.91 No.-

<P><B>Abstract</B></P> <P>Inflammation and thrombosis are linked to the use of polymer-based drug-eluting stents (DES). The aim of this study was to develop a polymer-free everolimus (EVL)-eluting stent using nitrogen-doped titanium dioxide (N-TiO<SUB>2</SUB>) and verify its efficacy by <I>in vitro</I> and <I>in vivo</I> assessment in a porcine coronary model. Various analytical approaches such as scanning electron microscopy and atomic force microscopy, electron spectroscopy, Fourier transform infrared spectrometry and contact angle measurement were employed for the characterization. As a part of biocompatibility assessment, platelet adhesion and smooth muscle cell (SMC) proliferation were examined. Bare metal stent (BMS), N-TiO<SUB>2</SUB> stent, everolimus-eluting N-TiO<SUB>2</SUB> (N-TiO<SUB>2</SUB>-EVL) stent, and commercialized EVL-eluting stent (EES) were randomly placed in forty coronary arteries in twenty pigs. After four weeks of implantation, the stents were subjected to histological and quantitative analysis. The N-TiO<SUB>2</SUB> film used in this study was well coated without any cracks or peeling. Surface hydrophilicity (88.8% of angle decrement) could be associated with the decrease in surface roughness post N-TiO<SUB>2</SUB> deposition (37.0%). The platelet adhesion on the N-TiO<SUB>2</SUB> surfaces was less than that on the BMS surface. The proliferation of SMC was suppressed in the N-TiO<SUB>2</SUB>-EVL group (30.2%) but not in the BMS group. In the animal study, the percent area restenosis was significantly decreased in the N-TiO<SUB>2</SUB>-EVL group compared to that in the BMS group. The results (BMS; 47.0 ± 11.00%, N-TiO<SUB>2</SUB>-EVL; 31.7 ± 10.50%, and EES; 29.1 ± 11.21%, <I>n</I> = 10, <I>p</I> < 0.05) were almost at par with those of the commercialized EVL-eluting stent. The introduction of N-TiO<SUB>2</SUB> deposition during fabrication of polymer-free DES may be an efficient accessorial process for preventing in-stent restenosis and thrombosis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> N-TiO<SUB>2</SUB> surfaces can help to reduce the platelet adhesion. </LI> <LI> In porcine model, N-TiO<SUB>2</SUB> everolimus decreased in-stent restenosis and fibrin deposition. </LI> </UL> </P>

In-line 마그네트론 스퍼터 장치를 사용하여 산소 분위기에서 제작한 Ag 박막의 특성

구대영,김원목,조상무,황만수,이인규,정병기,이택성,이경석,조성훈,Ku, Dae-Young,Kim, Won-Mok,Cho, Sang-Moo,Hwang, Man-Soo,Lee, In-Kyu,Cheong, Byung-Ki,Lee, Taek-Sung,Lee, Kyeong-Seok,Cho, Sung-Hun 한국재료학회 2002 한국재료학회지 Vol.12 No.8

A study was made to examine the electrical, compositional, structural and morphological properties of Ag thin films deposited by DC magnetron sputtering in $O_2$ atmosphere with deposition temperature from room temperature to 15$0^{\circ}C$ using in-line sputter system. The Ag films deposited at temperature above $100^{\circ}C$ in oxygen atmosphere gave a similar specific resistivity to and even lower oxygen content than those deposited using pure Ar sputter gas The Ag films deposited with pure Ar gas was mainly composed of crystallites with [111] preferred orientation, while, for those deposited in oxygen atmosphere, more than 50% of the volume was composed of crystallites with [100] orientation. The difference in the micro structure did not cause any difference in the specific resistivity of Ag films. The results showed that the transparent conducting oxide films and the Ag films could be processed sequentially in the same deposition chamber with careful control of deposition temperature, which might result in a cost reduction for constructing the large scale in-line deposition system.

忠州市 主幹線 道路邊 建築外部色彩에 關한 硏究

崔生吉,孫泰鎭,柳顯紀,辛同寅,梁富弘,尹勝照,朴碩鉉 忠州大學校 1998 한국교통대학교 논문집 Vol.33 No.2

In order to understand the influence of colours on the urban design of a city, a portion of the central Chung-Ju was chosen as the study area, and three cases were researched as colours. These surveyed colours are analysed in their hue, value and chroma. It was necessary to determine the colours of 272 buildings exterior in all in order formulate the findings presented in this study. The result of this study can be briefly summerised as the following ; 1) The trends in the use of colour turn out like this ; 55.3% of the building were Y(yellow), YR(yellow-green), R(red) Family in Hue, abour 33.9% of them were within 8.0-8.9 and 26.6% were within 7.0-7.9 on the value scale, and about 48.5% of them were within 1.0-1.9, 2.0-2.9 and 3.0-3.9 on the chroma scale. 1) For case 1 on the exterior colours of buildings, about 57.8% were Y and YR family in Hue, about 53.7% were 8.0-8.9 and 7.0-7.9 in Value, and about 49.6% of them were within 1.0-3.9 in chroma. 2) For case 2 on the exterior colours of buildings, about 52.4% were Y and R family in Hue, about 65.1% were 7.0-8.9 in Value, and about 46% were 1.0-2.9 in Chroma. 3) For case 3 on the exterior colours of buildings, about 55.7% were Y and YR Family in Hue, about 72.1% were 7.0-8.9 in Value, and about 31.1% were 2.0-2.9 in Chroma. In summary, the colours of the building surveyed in central Chung-Ju can be characerized as Y, YR, R, N family in Hue, 7.0-7.9, 8.0-8.9 in Value registered in 1.0-1.9, 2.0-2.9, 3.0-3.9 in Chroma. The observed exterior colours show a tendency toward Yellow in Hue, High in Value and low in Chroma. Generally, the majority of the colours is light rather than strong in tone.

癩患者에서 Clofazimine 治療後 皮膚變化

鄭祥立,金仁珠 慶北大學校 醫科大學 1982 慶北醫大誌 Vol.23 No.2

癩病 및 癩反應治療自的으로 clofazimine을 服用한 患者를 對象으로 皮膚色의 變化, 魚鱗癬樣皮膚의 發生 및 組織學的 變化를 관찰한 結果를 다음과 같이 要約한다. 對象患者는 總 102名으로 病歷期間은 10年 以上이 73例(71.6%)로 가장 많았으며 年齡은 40∼49歲가 30例(29.4%)로 가장 많았다. Clofazimine의 服用期間은 1∼2年이 41例(40.2%)로 가장 많았고 服用量은 癩病治療 目的인 경우가 34例(33.3%), 癩反應治療인 경우가 68例(66.7%)로 後者가 2倍나 많았다. Clofazimine 服用後 皮膚色의 變化는 2週부터가 53例(52.0%)로 가장 많았고 色素沈着은 4週부터가 49例(48.0%)로 가장 많았으며 大量服用할수록 빨리 나타났다. 各種 染色에 依한 病理組織學的인 變化로는 眞皮內에 황갈색의 結晶體와 脂肪物質을 볼 수 있었고 基底部에 melanin 色素가 增加되어 있었다. 魚鱗癬樣皮膚는 90例에서 發生하였으며 服用後 2個月부터가 37例(41.1%)로 가장 많았고 12例에서는 전혀 發生하지 않았다. Clofazimine (Lamprene or B663) is a phenazine congener that is used in leprosy, especially sulphone resistance and lepra reaction. Patients treated with the drug may develop red discoloration and dark brown pigmentation of the skin and ichthyosis. The author observed one hundred and two leprosy patients who had developed discoloration, pigmentation and ichthyotic skin after initiating clofazimine therapy, as well as the histopathological findings of those changes. The results are summarized as follows: The duration of the leprosy was mostly more than 10 years (71.6%). Males (75.5%) predominated over females (24.5%) by 3 times, and the highest incidence occurred in the age group between 40 and 49 years (29.4%). For 40.2% of the patients, the duration of the clofazimine treatment was between 1 and 2 years. The number of patients receving a higher dosage, 100-300㎎ per day, for control of a lepra reaction was two times more than those receiving a lower dosage, 300-400㎎ per week, for the general treatment of leprosy. Reddish discoloration was most frequently observed 2 weeks after beginning treatment in 53 patients (52.0%), and 12 of 14 patients who showed discoloration earlier(a week after treatment) were in the higher dosage group. Dark brown pigmentation was most frequently observed 4 weeks after beginning treatment in 49 patients (48.0%), and by the third month, 64 of 68 patients (94.1%) in the higher dosage group showed pigmentation compared to 25 of 34 patients (74.5%) in the lower dosage group. For histopathological evaluation of color-changed skin, various staining methods were used on unstained frozen sections, yellow brown crystals were scattered in the dermis of both discolored and pigmented skin, being more remarkable in the latter. With H & E stain, melanin pigments were markedly increased in the epidermal basal layer, and a faint yellow brown ghost was seen in the pigmented skin. With fat stains using oil-red-O and Sudan Ⅲ, reddish amorphous materials were scattered in and around the cytoplasm of the macrophages of the pigmented skin. Ichthyotic skin was observed in 90 patients, and most frequently 2 months after beginning treatment in 37 patients (41.1%), but it was never observed in 12 of all patients in spite of a continuous intake of the drug for more than a year, thus showing no significant difference between the different dosage groups.

國民學校兒童의 健康基準確立을 爲한 血液의 生化學的 및 血液學的 成分에 對한 調査硏究

金相仁,趙漢翊,朴明姬,金昇元,尹鍾求 대한의학협회 1979 대한의학협회지 Vol.22 No.8

류마티스 관절염 환자의 T세포 수용체 Vβ 유전자 레퍼토리 분석

정성수 ( Sung Soo Jung ),황관표 ( Kwan Pyo Hong ),김동욱 ( Dong Yook Kim ),김태환 ( Tae Hwan Kim ),이인홍 ( In Hong Lee ),전재범 ( Jae Bum Jun ),배상철 ( Sang Cheol Bae ),유대현 ( Dae Hyun Yoo ),김성윤 ( Seong Yoon Kim ),이은영 ( 대한류마티스학회 1996 대한류마티스학회지 Vol.3 No.1

목적: 류마티스 관절염(rheumatoid arthritis; RA)의 병인에 있어 중추적인 역할을 하는 것으로 알려진 T세포에 관한 연구가 최근에 관심의 초점이 되고 있으나, 현재까지 한국인을 대항으로 시행된 보고는 없다. 한국인 류마티스 관절염 환자의 관절병변부위에서 T세포 수용체(TCR)의 Vβ유전자 사용의 빈도를 검색하여 한국인 류마티스 관절염 환자에서 사용되는 빈도가 높은 유전자계를 찾는데 목적을 두었다. 방법: 이에 저자는 T세포에 관한 연구의 일환으로 류마티스 관절염 환자 3명과 정상인 4명을 대상으로 말초 혈액과 활액 T세포로부터 추출한 RNA를 이용하여 cDNA를 합성한뒤 cDNA를 주형을 Vβ family specific oligonucleotide를 시발체(primer)로하여 반정량적 역전사 연쇄중합반응(semiquantative RT-PCR)을 시행하여 T세포 수용체(T cell receptor; TCR) Vβ 레퍼토리를 분석하여 다음과 같은 결과를 얻었다. 결과: 1. 정상인 4명의 말초혈액에서 T세포 수용체 Vβ유전자 평균사용빈도는 Vβ7(8.68±3.20%), Vβ3(7.83±2.03%), Vβ(6.74±1.43%)의 순서로 빈도가 높았으며, HLA-DR4 양성군(3명)에서는 Vβ8(7.39±1.71%), Vβ2(7.31±2.30%), Vβ1(7.22±1.54%)의 순서로 빈도가 높았고, HLA-DR4 음성(1명)에서는 Vβ3(17.79%), Vβ4(11.41%), Vβ24(9.8%)의 순서로 사용빈도가 높았다. 2. 류마티스 관절염환자 3명의 말초 혈액에서는 Vβ16(6.90±1.81%), Vβ18(6.89±0.80%), Vβ14(6.58±0.65%)의 순서로 빈도가 높았다. HLA-DR4 양성 환자군에서는 Vβ16(7.52±2.06%), Vβ14(6.96±0.04%), Vβ22(6.78±0.18%)의 순서로 빈도가 높았다. 각각 환자의 결과를 보면 첫번째 환자(HLA-DR4 양성)는 Vβ16(8.98%), Vβ14(6.99%), Vβ22(6.91%)의 순서로 빈도가 높았고, 두번째 환자(HLA-DR13 양성)는 Vβ18(7.79%), Vβ20(5.98%), Vβ24(5.90%)의 순서로 빈도가 높았다. 이를 종합해 보면 류마티스 관절염 환자의 말초혈액에서 Vβ16, Vβ20, Vβ14, Vβ18유전자계가 제한되어 사용되었다. 3. 류마티스 관절염환자의 활액의 T세포에서 Vβ유전자 평균발현빈도는 Vβ20(7.39±1.77%), Vβ18(5.60±1.31%), Vβ12(5.56±1.77%)순으로 높았으며, HLA-DR4양성인 환자군에서는 Vβ12(6.56±0.58%), Vβ20(6.44±0.94%), Vβ4(5.30±0.49%)순으로 빈도가 높았으며, HLA-DR4음성 환자에서는 Vβ20(9.29%), Vβ19(7.57%), Vβ18(7.07%)의 순서로 빈도가 높았다. 각각 환자의 결과를 보면 첫번째 환자(HLA-DR4 양성)는 Vβ12(6.15%), Vβ20(5.77%), Vβ7(5.74%)의 순서로 빈도가 높았고, 두번째 환자(HLA-DR4 양성)는 Vβ20(7.10%), Vβ12(6.98%), Vβ24(5.83%)의 순서이었고, 세번째 환자(HLA-DR13 양성)는 Vβ20(9.29%), Vβ19(7.57%), Vβ18(7.06%)의 순서로 빈도가 높았다. 활액 T세포에서는 Vβ20, Vβ12유전자계가 제한되어 사용되었다. 4. 말초 혈액에 비해 활액 T세포에서 TCR Vβ유전자 사용빈도가 1.5배 이상인 경우는 Vβ5.2, Vβ9, Vβ23 이었으나, 이들 유전자계가 활액 T세포 전체에서 차지하는 사용빈도에 있어서는 각각 Vβ5.2는 1.07±1.26%, Vβ9는 2.17±1.42%, Vβ23는 3.84+1.97%로 낮은 수치를 나타내었다. 각각 환자에서 비교했을때에 첫번째 환자(HLA-DR4 양성)는 Vβ23(3.69%:1.12%), Vβ5.1(3.51%:1.40%), Vβ12(6.15%:3.29%), Vβ6(3.35%:2.10%), Vβ2(5.74%:3.73%)의 유전자계에서, 두번째 환자(HLA-DR4 양성)는 Vβ9(3.58%:0.74%), Vβ19(4.67%:1.52%), Vβ5.2(2.52%:0.87%), Vβ10(4.25%:1.75%), Vβ1(5.74%:2.35%)의 유전자계에세, 세번째 환자(HLA-DR13 양성)는 Vβ6(2.68%:0.15%) Vβ9(2.17%:0.73%), Vβ11(2.30%:1.10%), Vβ20(9.29%:5.98%)의 유전자계에서 활액에서 말초혈액보다 1.5배이상 증가되어 사용됨을 볼수 있었다. 즉 활액에서 말초혈액보다 의미 있게 편향되어 사용되는 Vβ 유전자계는 각각 환자마다 다른 결과를 나타내었다. 5. 류마티스 관절염환자의 말초 혈액과 정상인의 말초 혈액의 T세포의 Vβ 유전자 사용빈도를 비교했을때 류마티스 관절염 환자의 말초혈액 T세포에서 Vβ16(6.90±1.81%:3.23±1.21%), Vβ18(6.89±0.80%:2.72±0.70%) 유전자계가 2배 이상으로 빈도가 높았고, HLA-DR4양성군만 비교하였을때도 Vβ16(7.52±2.06%:3.69±1.46%), Vβ18(6.45±0.28%:2.61±0.86%) 유전자계가 2배 이상 사용이 많았다. 결론: 이상의 성적으로 미루어 보아 류마티스 관절염 각각 환자마다 다른 Vβ 유전자계의 제한적 사용과 편향됨을 볼 수 있었으나, 공통된 유전자계의 증식은 볼 수 없었다. 이러한 결과는 다른 연구보고와는 다른 결과를 보였고, 이것은 대상 환자의 질병의 유병기간이 다르고, 또 유전적인 배경, 생존 환경, 적용된 방법의 차이로 기인한다고 하는 기존의 보고와 부합된다고 사료되는데 질병의 진행단계에 따라 주로 면역반응을 일으키는 항원의 에피토프가 달라짐에 따라 여기에 대항하는 T세포들의 수용체도 이들 에피토프에 반응할 수 있는 수용체를 가진 코론들이 증식하게 된다는 epitope spreading theory에 부합되며, 이는 아주 초기에 질병을 시작하게 유도하는 항원의 특성을 규명하기 위해서는 환자선택이 연구결과에 결정적인 역할을 할 것으로 사료된다. 또 다른 가능성은 각각 환자마다 다른 Vβ유전자계의 증식된 클론들의 CDR3 연기 서열을 규명하여 비록 유전자 서열이 다를 지라도 항원과 결합하는 같은 성상을 갖는 아미노산 motif를 가질 가능성에 대해서도 연구가 필요하리라 사료된다. Objectives: Polymerase chain reaction (PCR) technology was eamine synovial fluid and peripheral T cells in patients with rheumatoid arthritis (RA) to determine the preferential usage of the T cell receptor(TCR) variable region (V) gene. Methods: Oligonucleotide primers specific for individual TCR Vβ gene families were used to amplify the TCR gene products in a semiquantitative assay of their relative utilization in unselected T cell populations. Results: The result of Vβ utilization was generally heterogenous, similar with previous reports. However, the mean expression of Vβ16 and Vβ18 in RA was more preferentially utilized compared to normal donors. The usage of Vβ in peripheral blood from 3 patients with RA demonstrated restrictions in Vβ16, Vβ20 and Vβ18 genes, respectively. Analyses of synovial fluid resulted in restriction in β12, Vβ20 and Vβ20, respectively. Although there was no significant pattern of skewed Vβ gene mean usage when comparing the synovial fluids with the peripheral blood T cells from RA patients, there were significant biased Vβ genes, Vβ12, Vβl and Vβ20, each 3 patients. As the HLA type is a determining factor in shaping TCR repertoire of peripheral T cells, we compared the Vβ utilization in HLA-DR4 expressing groups that have susceptibility and gene dosage effect in disease progression. It was a little different that comparing the pattern of Vβ usage in peripheral blood and synovial fluid from RA patients between HLA-DR4 positive and negative group. Conclusion: The results were consistent with the conclusion that the increased Vβ family T cells infiltrate synovium and are dependent on each patient and may be involved in inducing and maintaining the synovitis that characterizes RA The different outcome of each patient may be due to the difference in disease duration, genetic background and geographic region. A more important factor may be the stage of disease, because epitope induced immune reaction may change over time. Therefore, selecting patients early in the course of disease may be important and may facilitate the need for more in-depth TCR analysis in the future.

運動의 危險性

朴鱗基,白永守,鄭泰相 忠南大學校體育科學硏究所 1993 體育科學硏究誌 Vol.11 No.1

The purpose of this study was to introduce the risk of exercise to exersize testing or participation. The real and theoretical benefits of regular exercise have promoted this activity not only in healthy individuals. but also in cardiac patients. Exercise is assuming an increasingly important role in preventive medicine and cardiac rehabilitation. Apart from favorable effects on plasma lipids, there is often the experience of even greater psychological benefits, that is, improved in mood, reduced nxiety, and sense of well-being. A through medical evaluation is recommended before starting an exercise program for all individuals with known cardiovascular, pulmonary or metabolic disease. Individuals of any age considered at higher risk by the presence of diabetes, hypertension, family history of coronary disease, elevated cholesterol, or smoking may benefit from the screening exercise test before engaging in competitive or recreational sports. Sudden death in sport is a recently recongnized phenomenon for which little scientific information exists. Most previous investigation on sudden death in sport has concentrated on running, track and field sports. Death, directly related to sporting activities is rate. The total risk is low, but the relative risk is increased during vigorous exercise. Risk increases with age and with the intensity of exercise. The basis of clinical studies and our personal experience, most deaths during exercise in the adult population are probably preventable because they occur in individuals exercising under one or more of the following circumstances: no medical clearance, no supervison, the limits of the exercise prescription were exceed, the symptoms during exercise were disregarded. Orthopedic injuries appear to increase exponentially with jog-run types of activities in association with increased the total volume of work done in the program, such as, structural abnomalities, sudden large increases in training, running form, streching habits, warm-up, and cool-down may constitude risk factors. With high risk individuals, a physician should be present during exercise intensity, and energy equipment and qualified personnel should be avaiable for exercise testing of all patients in order to provide life saving emergency are, In addition to significant clinical signs and symptoms.

만성육아 종성염증에서의 T-cell 그리고 B-cell의 분포에 관한 연구

장인성,양승하,강상균 순천향대학교 1994 논문집 Vol.17 No.2

Tuberculosis, leprosy, foreign body reaction, parasite, and fungal infection are chronic granulomatous inflammation forming granuloma within lesion. There are much studies for the mechanism of cell mediated immume reaction in tuberculosis and leprosy, but the study using immune peroxidase stain in paraffin embedded tissue is rare. This study was carried out to observe the distribution of T cell and B cell in chronic granulomatious inflammation, such as tuberculosis 21 cases, leprosy 9 cases, cysticercosis 7 cases, and foreign body reaction 8 cases. Fourthy five cases of above diseases obtained by excisional biposy were processed for immune enzyme histologic stain by Avidin-Biotin complex method using MT1 and MB2. The results obtained are following as : 1. Tuberculosis showed positive reaction of MT1 in all within and outer granuloma among 21 cases, and MB2 in 2 cases and 14 cases respectively. 2. In 9 cases of leprosy lepromatous type showed positive reaction of MT1 in all 5 cases and MB2 in each 2 cases within and outer granuloma, tuberculoid and borderline types showed posittive reaction of MT1 and MB2 in all 2 cases respectively. 3. Positive reaction of MT1 in 7 cases of cysticercosis, revealed 6 cases within granuloma and all cases outer one, MB2 in 5 cases and 6 cases respectively. 4. Positive reaction of MT1 in 8 cases of foreign body reaction disclose 5 cases within granuloma and 7 cases outer one, and MB2 in 3 cases and 4 cases respectively. The above results showed T cell is related to main immune protective mechanism in granulomatous inflammation, and B cell reveal only weak reaction.

The Effect of Rosiglitazone on the Cell Proliferation and the Expressions of p27 and Skp2 in<i>Helicobacter pylori</i>Infected Human Gastric Epithelial Cells

Kim, Sung-Soo,Cho, Young-Seok,Kim, Hyung-Keun,Shin, Ok-Ran,Chae, Hiun-Suk,Choi, Myung-Gyu,Chung, In-Sik The Korean Society of Gastroenterology 2010 대한소화기학회지 Vol.55 No.4

<P>Background/Aims: Ligands for peroxisome proliferator-activated receptorgamma (PPARgamma), a member of the ligand-activated nuclear receptor superfamily, exhibit anti-tumoral effects and are associated with de novo synthesis of proteins involved in regulating the cell cycle and cell survival/death. Helicobacter pylori (H. pylori) is an etiologic agent for gastric adenocarcinoma, and raises the cell turnover of gastric epithelium. The aim of this study was to investigate the effect of PPARgamma ligand rosiglitazone on the cell proliferation and the expressions of p27 and Skp2 protein in H. pylori infected gastric epithelial cells. Methods: We examined the expression of PPARgamma by Western blot in H. pylori infected AGS human gastric epithelial cells. The effect of rosiglitazone on the survival of H. pylori infected AGS cells was assessed by cell viability assay. After the treatment of rosiglitazone in H. pylori infected AGS cells, the expressions of p27 and Skp2 were assessed by Western blot. Results: The expression of PPARgamma protein was increased in H. pylori infected AGS cells. Cell growth was inhibited and decreased in dose- and time- dependent manner in H. pylori infected AGS cells treated with rosiglitazone. A decrease in Skp2 expression and a reciprocal increase in p27 expression were found in dose- and time-dependent manner in H. pylori infected AGS cells treated with rosiglitazone. Conclusions: Rosiglitazone inhibited the growth of H. pylori infected AGS cells. Rosiglitazone attenuated Skp2 expression, thereby promoting p27 accumulation in H. pylori infected human gastric epithelial cells. Further studies will be needed to find the effects of accumulation on cell turnover in H. pylori infection and the role in the H. pylori-associated gastric carcinogenesis.</P>