경북 동부지역 번식장애 소에서의 큐열 항체 보유율

우인옥 ( In Ohk Ouh ),서민구 ( Min Goo Seo ),장영술 ( Young Sul Jang ),김상윤 ( Sang Yun Kim ),곽동미 ( Dong Mi Kwak ) 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.4

This study was done to determine the seroprevalence of Coxiella burnetii in cattle with reproductive disorders reared in eastern Gyeongbuk province. A total of 146 cattle blood samples from 83 farms in eastern Gyeongbuk province were collected in 2011∼2012. Among them, 17 (11.6%) samples from 11 (13.3%) farms were seropositive for C. burnetii by ELISA. In dairy cattle with mastitis, 15 (38.5%) milk samples from 9 (42.9%) farms were seropositive among 39 milk samples from 21 farms. Among the seropositive farms, one farm with high seroprevalence was selected to assess the seroprevalence of C. burnetii in the bulk-tank milk (BTM) and blood samples of dairy cattle. The BTM sample from the farm was seropositive to C. burnetii and 6 (15%) out of 40 dairy cattle were seropositive. In cattle with reproductive disorders including infertility, abortion and stillbirth, 2 (1.9%) samples from 2 (3.2%) farms were positive among 107 samples collected from 62 farms. Among 2 positive samples, one of them is dariy cattle (100%) and the other is Korean cattle (0.9%) (P<0.001). This suggests that Korean cattle was relatively resistant to C. burnetii. Based on the data obtained, we identified association between C. burnetii shedding in milk and chronic subclinical mastitis in dairy cattle. Since seroprevalence of C. burnetii is relatively high in dairy cattle, further intensive studies on C. burnetii among the high risk milk farms and dairy cattle herds are needed to evaluate infection status.

경북지역 집합유와 젖소에서 큐열 항체 보유율 조사

우인옥 ( In Ohk Ouh ),서민구 ( Min Goo Seo ),도재철 ( Jae Cheul Do ),김인경 ( In Kyoung Kim ),조민희 ( Min Hee Cho ),곽동미 ( Dong Mi Kwak ) 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.4

Q fever is a rickettsial infection caused by Coxiella burnetii that is transmitted from animals to humans. Modes of transmission to humans include inhalation, tick bites and ingestion of unpasteurized milk or dairy products. This survey was aimed at monitoring the seroprevalence of C. burnetii in bulk-tank milk (BTM) in Gyeongbuk province. In addition, the seroprevalence of C. burnetii was investigated at the herd level of dairy cattle in eastern Gyeongbuk province in which many dairy cattle are reared. Among 324 BTM samples collected from 20 country areas, 175 (54%) BTM samples from 15 (75%) country areas were positive for C. burnetii by ELISA. By regions, the seroprevalence of BTM samples in eastern, central, western and northern areas of Gyeongbuk province were 62.7%, 48.4%, 45.1%, and 41.4%, respectively. When analyzed in the dairy cattle reared in the eastern area where high seroprevalence occurred in BTM samples, 119 (24.2%) out of 492 dairy cattle were positive for C. burnetii. Seroprevalence of C. burnetii in dairy cattle was increased with daily milk yield of farm (P<0.05) and age (P<0.001). Since seroprevalence of C. burnetii is relatively high in both BTM samples and dairy cattle reared in Gyeongbuk province, further studies on the high risk farms and herds are needed to evaluate infection status and appropriate control programs in this region.

실험견의 급성 저 산소성 폐고혈압중에서 내피세포 기원 확장인자 ( EDRF ) 의 역할

김인옥(In Ohk Kim),인광호(Kwang Ho In),조재연(Jae Yun Cho),이진구(Jin Goo Lee),심재정(Jae Jung Shim),강경호(Kyung Ho Kang),유세화(Se Hwa Yoo) 대한내과학회 1994 대한내과학회지 Vol.47 No.6

N/A Objectives : Hypoxic pulmonary vasoconstriction has been well documented in the pulmonary cirulation. However the mechanism and the modulating factors of the hypoxic pulmonary vasoconstriction remain unclarified. With the recent evidences that the endothelium-de- rived relaxing factor (EDRF) is an important mediator of vascular tone, there have been increasing interests in defining the role of the EDRF as a potential mediator of hypoxic pulmonary vasoconstriction. But the results of studies on the role of EDRF during hypoxic pulmonary vasoconstriction have been inconsistent, and the studies in the intact animals are very rare. To investigate the role of EDRF during pulmonary hypertension induced by hypoxia, we measured changes of hemodynamic parameters after adding NO synthesis inhibitor and its substrate. Methods : Five dogs were anesthesized with thiopental sodium. After intubation with an endotracheal tube, the dogs were mechanically ventilated with a Harvard volume-cycled animal ventilator. Venous and arterial catheters were placed in the limb vein and femoral artery. Swan-Ganz catheter was inserted via right internal jugular vein for measuring pulmonary arterial pressure and pulmonary capillary wedge pressure, and cardiac output. We measured the changes of hemodynamic parameters in normoxia and hypoxia and the effects of L-NNA (30 mg/kg) and L-arginine (200 mg/kg). Results : 1) Hypoxia caused a significant increase in pulmonary arterial pressure. 2) The infusion of L-NNA in normoxic state did not change any of the hemodynamic variables. 3) With the infusion of L-NNA, the increase in pulmonary arterial pressure produced by hypoxia were significantly augmented. 4) With the infusion of L-arginine, hypoxic pulmonary hypertension augmented by L-NNA was significantly reversed, Conclusion : L-NNA did not affect the pulmonary arterial pressure in normoxic state, but augmented the pulmonary hypertension during hypoxia and L-arginine reversed the hypoxic pulmonary hypertension almost to control level. It is suggested that diminished EDRF formation contributes greatly to the pulmonary hypertension induced by hypoxia.

Molecular detection of <i>Anaplasma phagocytophilum</i>-like <i>Anaplasma</i> spp. and pathogenic <i>A. Phagocytophilum</i> in cattle from South Korea

Seo, Min-Goo,Ouh, In-Ohk,Kwon, Oh-Deog,Kwak, Dongmi Elsevier 2018 Molecular phylogenetics and evolution Vol.126 No.-

<P><B>Abstract</B></P> <P> <I>Anaplasma phagocytophilum</I> is the causative agent of human granulocytic anaplasmosis and tick-borne fever in domestic ruminants. Differential diagnosis of zoonotic and pathogenic tick-borne diseases like granulocytic anaplasmosis is important for the efficient implementation of control programs. Thus, the differentiation of pathogenic <I>A. phagocytophilum</I> from non-pathogenic <I>A. phagocytophilum</I>-like (APL) <I>Anaplasma</I> spp. is essential. Recent molecular analyses of APL revealed its distinct phylogenetic position from <I>A. phagocytophilum</I>. This study was conducted to detect <I>A. phagocytophilum</I> and genetically related strains in 764 cattle in South Korea using PCR and restriction fragment length polymorphism assays. APL clade A and <I>A. phagocytophilum</I> were identified in 20 (2.6%) and 16 (2.1%) cattle, respectively, with 16 cattle (2.1%) displaying co-infection. The 16S rRNA sequences of APL clade A were similar (98.3–99.9%) to those clustered in the APL clade A from eastern Asia. The <I>A. phagocytophilum</I> 16S rRNA sequence shared 98.6–100% identity to those of the <I>A. phagocytophilum</I> group. We used PCR to amplify the <I>groEL</I> and <I>msp2</I> genes from the 20 samples positive for the 16S rRNA gene and found that 16 were positive for the <I>groEL</I> sequences in the APL clade A, which showed identity (82.8–84.4%) to those clustered in the APL clade A from Japan. Amplification of <I>msp2</I> was unsuccessful. The co-infection results suggested sequence diversity in <I>Anaplasma</I> spp. Till date, both <I>A. phagocytophilum</I> and APL have been reported to be distributed separately in several animals throughout South Korea. This report is the first co-detection of <I>A. phagocytophilum</I> and APL in Korean cattle using molecular methods. Further studies are needed to provide additional molecular background and trace the evolutionary tree of <I>Anaplasma</I> species in animals and ticks.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Genotyping of <I>A. phagocytophilum</I> (AP) and <I>A. phagocytophilum</I>-like (APL) strains. </LI> <LI> Sixteen AP and 20 APL were detected in 764 cattle by 16S rRNA sequencing and RFLP assay. </LI> <LI> The APL strains were more divergent in the <I>groEL</I> gene than in the 16S rRNA gene. </LI> <LI> Amplification of the <I>groEL</I> and <I>msp2</I> gene was unsuccessful in AP. </LI> <LI> First detection of AP and APL in Korean cattle and possible circulation of new APL. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Increased immunoreactivity of c???Fos in the spinal cord of the aged mouse and dog.

Ahn, Ji Hyeon,Shin, Myoung Chul,Park, Joon Ha,Kim, In Hye,Lee, Jae-Chul,Yan, Bing Chun,Hwang, In Koo,Moon, Seung Myung,Ahn, Ji Yun,Ohk, Taek Geun,Lee, Tae Hun,Cho, Jun Hwi,Shin, Hyung-Cheul,Won, Moo-H SPANDIDOS PUBLICATIONS 2015 MOLECULAR MEDICINE REPORTS Vol.11 No.2

<P>Expression of c-Fos in the spinal cord following nociceptive stimulation is considered to be a neurotoxic biomarker. In the present study, the immunoreactivity of c-Fos in the spinal cord was compared between young adult (2-3 years in dogs and 6 months in mice) and aged (10-12 years in dogs and 24 months in mice) Beagle dogs and C57BL/6J mice. In addition, changes to neuronal distribution and damage to the spinal cord were also investigated. There were no significant differences in neuronal loss or degeneration of the spinal neurons observed in either the aged dogs or mice. Weak c-Fos immunoreactivity was observed in the spinal neurons of the young adult animals; however, c-Fos immunoreactivity was markedly increased in the nuclei of spinal neurons in the aged dogs and mice, as compared with that of the young adults. In conclusion, c-Fos immunoreactivity was significantly increased without any accompanying neuronal loss in the aged spinal cord of mice and dogs, as compared with the spinal cords of the young adult animals.</P>

울릉도 소의 전염성 병원체 감염률 조사

서민구 ( Min Goo Seo ),도재철 ( Jae Cheul Do ),우인옥 ( In Ohk Ouh ),조민희 ( Min Hee Cho ),김중규 ( Joong Kew Kim ),김영환 ( Young Hoan Kim ),박노찬 ( No Chan Park ),곽동미 ( Dong Mi Kwak ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.4

Prevalence of infectious agents, including Brucella abortus (BA), Mycobacterium bovis (MB), bovine leukemia virus (BLV), M. avium subsp. paratuberculosis (MP), Neospora caninum (NC) and Toxoplasma gondii (TG), was investigated in all the cattle raised in Ulleung island during 2007∼ 2010. For BA, the prevalences in head and farm were 8.1% (44/545) and 5.5% (4/73) in 2007, all negative in 2008∼2009, and 0.5% (4/774) and 1.7% (1/58) in 2010, respectively. For MB, no sample was positive by PPD or ELISA in 2007∼2010. For BLV and MP, no sample was positive by ELISA in 2007∼2009. For NC, seroprevalences in head and farm were 0.2% (1/545) and 1.4% (1/73), respectively, in 2007 and all negative in 2008∼2009. For TG, seroprevalences in head and farm were 17.6% (97/552) and 54.8% (34/62) by ELISA in 2009. By regions, the seroprevalences of TG in Ulleung-eup, Seo-myeon and Buk-myeon were 26.0%, 9.8% and 16.7%, respectively, which had significant differences (P<0.0001). Tiger cattle were more resistant to TG infection than Hanwoo. The seroprevalence of TG in summer was higher than in autumn. The seroprevalence of TG in cows was higher than in oxen. The seroprevalence of TG in cattle was increased with age. In conclusion, this study indicates that the prevalences of six infectious diseases, except for TG which are widely spread, are relatively low in cattle reared in Ulleung island.

Antibody responses after vaccination against equine influenza virus in the Republic of Korea in 2019

In-Ohk Ouh,Min Ji Kim,Hyeonhae Choi,Hyoung seok Yang,Sang Kyu Lee,Seo Young Moon,Suyeong Yun,Bang-hun Hyun,Soo dong Cho,Choi-Kyu Park,Yoon-Hee Lee 한국예방수의학회 2020 예방수의학회지 Vol.44 No.3

In this study, antibody responses after vaccination against equine influenza were investigated among 1,591 horses in the Republic of Korea using the hemagglutination inhibition (HI) test. Equine influenza has not occurred since 2011 and a commercial vaccine against H3N8 has been used. The equine influenza virus, A/equine/South Africa/4/03 (H3N8), was used as the antigen in the HI assay. The mean seropositive rate was 90.5% in 2019. Except for stallion whose seropositive rate was 78.5%, all seropositive rates of other horse types were over 90%. Regionally, except for Gangwon-do and Jeju-do whose seropositive rates were 89.0% and 87.1%, all seropositive rates in other provinces were over 90%. In the future, more through vaccination against equine influenza needs to be done based on this investigation result.

Seroprevalence of the swine influenza A virus in domestic pigs in the Republic of Korea

In-Ohk Ouh,Seo Young Moon,Mi-Ja Park,Min Ji Kim,Min-Ho Seong,Jong-Sik Bak,Hyoung seok Yang,Hyeonhae Choi,Suyeong Yun,Bang-hun Hyun,Soo dong Cho,Yoon-Hee Lee,Choi-Kyu Park 한국예방수의학회 2020 예방수의학회지 Vol.44 No.3

Influenza A virus (IAV) causes respiratory disease in birds and mammals, including pigs and humans. Infection by IAV in pigs increases not only economic losses in the swine industry but also the emergence of novel IAV variants via gene reassortment, which is important due to the susceptibility of both birds and humans to IAV. This study provides serological data obtained during a study to detect IAV infections in pigs in the Republic of Korea during 2018 and 2019. A total of 1,559 samples were collected from 74 domestic pig farms. Hemagglutination inhibition (HI) assays were performed using the A/Swine/Korea/25-13(H1N1), A/Swine/Korea/E102 (H1N2), and A/Swine/Korea/Cy10/2007 (H3N2) viruses as antigens. The HI assay results showed that 266 of the 1,559 samples were seropositive (17.0%). Among these, H1N1, H1N2, and H3N2 comprised 7.3% (114), 6.0% (93), and 8.8% (137) of the 1,559 samples, respectively. Co-infections of H1N1/H1N2, H1N1/H3N2, H1N2/H3N2 and H1N1/H1N2/H3N2 were observed in 2.1% (31), 1.5% (23), 1.5% (24), and 0.8% (13) of the 1,559 samples, respectively. Interestingly, IAV infections were detected in all nine provinces of the country.

Focal cerebral ischemic injury decreases calbindin expression in brain tissue and HT22 cells

In-Ohk Ouh,Young-Min Kim,Phil-Ok Koh 한국실험동물학회 2013 Laboratory Animal Research Vol.29 No.3

Calbindin is a calcium binding protein that controls intracellular calcium levels and has a neuroprotective function against apoptotic stimuli. We investigated the expression of calbindin in ischemic brain injury. Focal cerebral ischemia was induced in male rats by middle cerebral artery occlusion (MCAO) and cerebral cortices were collected 24 h after MCAO. Cerebral ischemia significantly increased infarct volume. RT-PCR and Western blot analyses showed that MCAO injury induced a decrease of calbindin expression. Moreover, immunohistochemical staining showed that the number of calbindin-positive cells decreased in ischemic regions of MCAO-operated animals. In cultured hippocampal-derived cell lines, glutamate exposure increased intracellular Ca<SUP>2+</SUP> concentrations and decreased calbindin expression. Taken together, both in vivo and in vitro results demonstrated decreases of calbindin after neuronal cell injury. These results suggest that decreases of calbindin in ischemic brain injury contribute to neuronal cell death.

Surveillance of canine influenza A virus in the Republic of Korea from 2016 to 2021

Seong-In Lim,Min Ji Kim,In-Ohk Ouh,Min-Ji Kim,MiJung Kwon,Gyeong Gook Park,Eui Hyeon Lim,Eun-Ju Kim,Yeun-Kyung Shin,Soo Dong Cho,Bang-hun Hyun,Yoon-Hee Lee 한국예방수의학회 2022 예방수의학회지 Vol.46 No.4

Influenza A viruses (IAVs) are members of the family Orthomyxoviridae and genus Orthomyxovirus. Avian and mammalian species are the host of IAVs, which includes humans and dogs. Canine influenza virus (CIV) is an emerging pathogen that causes severe and acute respiratory diseases in dogs. This study monitored the antigen and antibody against CIV in dogs in the Republic of Korea (ROK) from 2016 to 2021. One thousand and seventy-two nasal swabs and 1,545 blood samples were collected from animal hospitals and animal shelters. Five nasal swabs in 2017 and seven in 2018 from stray dogs were positive for CIV according to RT-PCR. The prevalence of H3N2 CIV ranged from 9.5% to 24.8%, according to the hemagglutination inhibition (HI) assay. On the other hand, none of the serum samples from 2018 to 2021 showed seropositivity against the avian H5, H7, and H9 viruses. The HI titers for H3N2 ranged from 16 to 512. The distribution of HI titer 16–32 was 57.6% in seropositive samples. The pet dogs were vaccinated against CIV, but the stray and military dogs were unvaccinated. In 2017 and 2018, the seroprevalence of CIV in stray dogs was higher than in the other years, and viral RNA was detected in nasal swabs. It may mean previous exposure of stray dogs to CIV. With the increasing number of pet dogs and the close contact between humans and dogs, canines could serve as an intermediate host for transmitting IAVs to humans. Therefore, continuous surveillance of CIV is needed for public health and the potential emergence of novel zoonotic viruses.