방풍의 항염 효과 기전

노성일 ( Sung Il Noh ),김상돈 ( Sang Don Kim ),박성철 ( Sung Cheul Park ),서병윤 ( Byung Yun Seo ),염승룡 ( Seung Ryong Yeom ),권영달 ( Young Dal Kwon ),신병철 ( Byung Cheul Shin ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2005 한방재활의학과학회지 Vol.15 No.2

목적 : 방풍은 임상적으로 관절염을 포함한 다양한 염증성 질환 치료제로 사용되어 왔다. 본 연구에서는 인간 비만세포를 이용하여 세포 독성에 영향을 주지 않는 농도에서 방풍의 항염 효과 및 그 기전을 검토했다. 방법 : 인간의 HMC-1세포를 IMDM에서 페니실린, 스트렙토마이신, 모노티오글리세린를 첨가하여 배양하고 방풍추출액을 투여하였다. 그 다음 MTT, ELISA, RT-PCR, 세포내 칼슘측정, 핵단백분석을 이용하여 TNF-α, IL-6, IL-8 각각의 형성과 mRNA발현, 세포내 칼슘 수준, NF-κB 발현에 대한 방풍추출액의 반응을 측정하고 통계처리 하였다. 결과 : 방풍은 PMA와 calcium ionophore A23187로 활성화된 비만세포에서 세포내 칼슘 수준과 NF-κB, TNF-α와 IL-6의 발현을 억제 시켰고 RT-PCR을 이용한 mRNA 발현에서 TNF-α와 IL-6의 발현을 억제하였다. 결론 : 방풍은 비만세포내 칼슘 수준 및 NF-κB의 활성을 억제하고 염증성 세포 활성 물질인 TNF-α와 IL-6의 분비도 억제하여 항염 효과를 나타냄을 암시하고 있다.

폐석산의 관광자원화와 경제적 효과 분석에 관한 연구

주우일(Joo, Woo?Il),권현철(Kweon, Hyeon?Cheol),김남룡(Kim, Nam?Ryong) 한국주거환경학회 2009 주거환경(한국주거환경학회논문집) Vol.7 No.2

Geochang-gun is one of Korea’s top 3 granite production areas along with Iksan city and Pocheon city and there are 16 quarries in its region. The amount of gathering rough stones in those 16 quarries is commonly getting reduced every year. As of February, 2009, the quarry under operation are just about 5 and 11 quarry closed their business or still remain idle. So, the possibility is high that the 11 quarries will be translated to disused quarries sooner or later. Thus, it is necessary to find their effective value and seek a way for making them a resource economically. As a way, this study suggested a direction which can make disused quarries a tourism resource. That is to say, the study introduced the facilities which can link the disused quarries to both the rocky walls of the almost vertical rocks gathered and Mt. Keumwon Forest after fully reviewing the target area"s neighboring conditions. District A in the plan for disused quarry consists of recreational, gardening and convenient facilities and while District B in the plan for disused quarry is formed of athletic, gardening and convenient facilities, bearing a different character each other. The budget for making those disused quarry covering 4,028㎡ in Geochang region a resource will be expected to be about 3.518 billion won except land purchase expense. As a result, the study found that such development will result in approximately 5.224 billion won of production inducement and 672 million won of value added in an economic effect aspect. Also, it showed that the development create new 377 jobs and hire 329 persons at the same time. Anyhow, the financial self-supporting ratio of Geochang-gun is meager 13.5%, which is short of the average of the gun-unit bodies in South Gyeongsang Province, showing a very poor financial level. In order to raise such a poor financial self-supporting ratio, Geochang-gun should make full of the resources which it holds and establish the ways which can build an economic base. As a part of the ways, I suggest the development of disused quarry and I could prove the economic ripple effect of such development through this study.

Ameliorating effect of dipotassium glycyrrhizinate on an IL-4- and IL-13-induced atopic dermatitis-like skin-equivalent model

Lee, Sung Hoon,Bae, Il-Hong,Choi, Hyangtae,Choi, Hyeong Won,Oh, Soojung,Marinho, Paulo A.,Min, Dae Jin,Kim, Dae-Yong,Lee, Tae Ryong,Lee, Chang Seok,Lee, Jongsung Springer-Verlag 2019 Archives of dermatological research Vol.311 No.2

고려홍삼의 수지상세포 활성화 효과

김도순(Do-Soon Kim),박정은(Jueng-Eun Park),서권일(Kwon-Il Seo),고성룡(Sung-Ryong Ko),이종원(Jong-Won Lee),도재호(Jae-Ho Do),이성태(Sung-Tae Yee) 고려인삼학회 2006 Journal of Ginseng Research Vol.30 No.3

본 연구에서는 정관장 홍삼의 물(water) extract, 식용발효 주정 extract 및 홍삼 추출물로부터 분리 제조한 crude saponin을 이용하여 면역반응을 매개하는 수지상세포의 활성효과에 대하여 알아보았다. 그 결과 홍삼시료 중, crude saponin 100 ㎍/㎖을 처리하였을 때 수지상세포의 세포표면분자인 MHC class II, CD40, CD80, CD86의 발현이 증가하였으며, phagocytosis는 감소하였다. 또한 홍삼시료를 처리한 수지상세포와 allogeneic T세포를 함께 배양하였을 때, 홍삼시료의 물 extract, 식용발효주정 extract, crude saponin 모두 allogeneic T세포의 증식반응을 유도하였고, IL-2와 IFN-γ의 생산량을 증가시키는 것을 확인하였다. 또한 CD4? syngeneic T세포와 CD8? syngeneic T세포의 반응에서도 T세포의 증식반응을 높게 유도하였으며, CD4? syngeneic T세포에서 IL-2와 IFN-γ의 생산량을 증가시키고, CD8? syngeneic T세포에서는 IFN-γ 생산량을 증가시키는 것을 확인하였다. 이상의 결과로 crude saponin의 경우 수지상세포의 세포표면 공동자극분자의 발현을 유도하고 성숙을 유도함으로써 T세포의 활성을 증진시키는 것으로 생각되며, 물 extract와 식용발효주정 extract는 crude saponin과는 다른 기작으로 T세포 활성화를 유도하는 것을 알 수 있었다. 따라서 실험에 사용한 홍삼시료, 즉 물 extract, 식용발효주정 extract, crude saponin 모두 수지상세포의 활성을 유도하는 물질로써 암항원 특이적 T세포 활성화를 이용한 항암치료에 이용할 수 있는 가능성이 있다고 사료된다. Ginseng is a medicinal herb widely used in Asian countries. Dendritic cells(DCs) play a pivotal role in the initiation of T cell-mediated immune responses, making them an attractive cellular adjuvant for use in cancer vaccines. In this study, we examined the effects of Red-ginseng(water extract, edible and fermented ethyl alcohol extract, crude saponin) on the DCs phenotypic and functional maturation. Immature DCs were cultured in the presence of GM-CSF and IL-4, and the generated immature DCs were stimulated by water extract, edible and fermented ethyl alcohol extract, crude saponin and LPS, respectively, for 24hours. The expression of surface co-stimulatory molecules, including MHC(major histocompatibility complex) class II, CD40, CD80 and CD86, was increased on DCs that were stimulated with crude saponin, but antigen-uptake capacity was decreased. The antigen-presenting capacity of Red-ginseng extracts-treated DCs as analyzed by allogeneic T cells proliferation and IL-2, IFN-γ production was increased. Furthermore, CD4? and CD8? syngeneic T cell(OVA-specific) proliferation and IFN-γ production was significantly increased. However, CD4? syngeneic T cell secreted higher levels of IL-2 in responding but not CD8? syngeneic T cell. These results indicate the immunomodulatory properties of Red-ginseng extracts, which might be therapeutically useful in the control of cancers and immunodeficient diseases through the up-regulation of DCs maturation.

Effect of Cytokines and bFGF on the Osteoclast Differentiation Induced by 1α,25-(OH)<SUB>2</SUB>D<SUB>3</SUB> in Primary Murine Bone Marrow Cultures

Han-Jung Chae,Jang-Sook Kang,Byung-Gwan Bang,Seoung-Bum Cho,Jo-IL Han,Joo-Young Choi,Hyung-Min Kim,Soo-Wan Chae,Hyung Ryong Kim 대한생리학회-대한약리학회 1999 The Korean Journal of Physiology & Pharmacology Vol.3 No.6

<P> Bone is a complex tissue in which resorption and formation continue throughout life. The bone tissue contains various types of cells, of which the bone forming osteoblasts and bone resorbing osteoclasts are mainly responsible for bone remodeling. Periodontal disease represents example of abnormal bone remodeling. Osteoclasts are multinucleated cells present only in bone. It is believed that osteoclast progenitors are hematopoietic origin, and they are recruited from hematopoietic tissues such as bone marrow and circulating blood to bone. Cells present in the osteoclast microenvironment include marrow stromal cells, osteoblasts, macrophages, T-lymphocytes, and marrow cells. These cells produce cytokines that can affect osteoclast formation. In vitro model systems using bone marrow cultures have demonstrated that IL-1β, IL-3, TNF-α, bFGF can stimulate the formation of osteoclasts. In contrast, IL-4 inhibits osteoclast formation. Knowledge of cytokines and bFGF that affect osteoclast formation and their capacity to modulate the bone-resorbing process should provide critical insights into normal calcium homeostasis and disorders of bone turnover such as periodontal disease, osteoporosis and Paget s disease.

Cell source-dependent <i>in vivo</i> immunosuppressive properties of mesenchymal stem cells derived from the bone marrow and synovial fluid of minipigs

Lee, Won-Jae,Hah, Young-Sool,Ock, Sun-A.,Lee, Jae-Hoon,Jeon, Ryong-Hoon,Park, Ji-Sung,Lee, Sang-Il,Rho, Na-Young,Rho, Gyu-Jin,Lee, Sung-Lim Elsevier 2015 Experimental cell research Vol.333 No.2

<P><B>Abstract</B></P> <P>The <I>in vitro</I> differentiation and immunosuppressive capacity of mesenchymal stem cells (MSCs) derived from synovial fluid (SF-MSCs) and bone marrow extract (BM-MSCs) in an isogenic background of minipigs were comparatively analyzed in a collagen-induced arthritis (CIA) mouse model of rheumatoid arthritis (RA). The proliferation capacity and expression of pluripotent transcription factors (Oct3/4 and Sox2) were significantly (<I>P</I><0.05) higher in SF-MSCs than in BM-MSCs. The differentiation capacity of SF-MSCs into adipocytes, osteocytes and neurocytes was significantly (<I>P</I><0.05) lower than that of BM-MSCs, and the differentiation capacity of SF-MSCs into chondrocytes was significantly (<I>P</I><0.05) higher than that of BM-MSCs. Systemic injection of BM- and SF-MSCs significantly (<I>P</I><0.05) ameliorated the clinical symptoms of CIA mice, with SF-MSCs having significantly (<I>P</I><0.05) higher clinical and histopathological recovery scores than BM-MSCs. Furthermore, the immunosuppressive properties of SF-MSCs in CIA mice were associated with increased levels of the anti-inflammatory cytokine interleukin (IL)-10, and decreased levels of the pro-inflammatory cytokine IL-1β and osteoclast-related sRANKL. In conclusion, SF-MSCs exhibited eminent pluripotency and differentiation capacity into chondrocytes, addition to substantial <I>in vivo</I> immunosuppressive capacity by elevating IL-10 and reducing IL-1β levels in CIA mice.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Immunosuppressive capacity of BM-, SM-, and SF-MSCs was evaluated in an RA model. </LI> <LI> Proliferation, pluripotency and chondrogenic differentiation capacity were higher in SF-MSCs. </LI> <LI> SF-MSCs exhibited improved therapeutic effects than BM-MSCs. </LI> <LI> SF-MSCs may have applications as immunosuppressive therapy in autoimmune diseases. </LI> </UL> </P>

加算平均應答을 利用한 誘發電位 檢出에 관한 硏究

崔一泓,金子龍 진주여자전문대학 1992 論文集 Vol.15 No.-

This paper is concerned with the detection system of the evoked potential using the method of averaging response. The detection system is implemented with the microprocessor. The evoked potential by the aperiodic photic stimulus is detected. A satisfactory result could be obtained from the detected evoked potential.

疎水性 中空絲膜을 이용한 Cu(II)의 透過抽出

李龍珍,金萬壽,朴東源,金英一,黃德基 東亞大學校 大學院 1997 大學院論文集 Vol.22 No.-

Liquid-liquid extractions by use of microporous hollow fiber modules are fast compared with conventional extraction equipment because of the large surface area per volume. In these modules, the extractant and feed can be contacted at high speed andtwo flows are completely independent, so there are no problems with loading and channeling. In this paper, it was investigated the extractability for liquid-liquid extraction of Cu(Ⅱ) from dilute aqueous solution into D2EHPA(di-2-ethylhexylphosphoric acid), TBP(tri-n-butyphosphate) and TOA(tri-n-octylamine) as organic extractants by using the hydrophobic hollow fiber module. To determine the dependence of mass transfer rate on distribution of Cu(Ⅱ) in the module, we compared the overall mass transfer coefficient with distribution ratio. From these experiments, we identified the effect of distribution ratio on the overall mass transfer coefficient was, for the low H, the K? was increased with increasing H, but high value of H, the effect of H on K? could be negligible.

실관막을 이용한 중금속 추출에서 물질전달에 미치는 분배비의 영향

李龍珍,金英一,陳道源,黃德基,朴東源 동아대학교 공과대학부설 생산기술연구소 1997 生産技術硏究所硏究論文集 Vol.2 No.1

The liquid-liquid extractions by use of microporous hollow fiber membrane modules are fast compared with conventional extraction equipment. Because they have a large surface area per volume. Solute diffuses out of the feed solution, through the fibers' porous wall, and into the extractant. The extractant and feed can be contacted at high speed, there are no problems with flooding, loading and channeling. Moreover, these modules should be characterized by low investment and operating costs because of the reduced hardware and the favorable hydrodynamics which minimizes concentration polarization and membrane fouling. In this paper, it was investigated the extraction selectivities for liquid-liquid extraction of heavy metal(Cd(Ⅱ)Co(II)) from dilute aqueous solution into the eatractants(EHPNA and TBP) by using the hydrophobic hollow fiber module. We also examined the effect of distribution ratio on the overall mass transfer coefficients.

미로매독의 임상청각학적 관찰

설승룡,이동훈,백무진,오일준,전경명 釜算慶南耳鼻咽喉科學會 1991 임상이비인후과 Vol.2 No.1