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Hyun, K.,Han, S.W.,Koh, W.G.,Kwon, Y. Pergamon Press ; Elsevier Science Ltd 2015 International journal of hydrogen energy Vol.40 No.5
In this research, we suggest an enzyme immobilization structure that glucose oxidase (GOx) is coated on carbon nanotube (CNT) and quantify an optimal condition for the immobilization. Physical adsorption of GOx to CNT is used as the method for GOx immobilization on CNT (GOx/CNT). Cyclic voltammetry (CV) is served to evaluate catalytic activity and direct electrochemistry, while SEM is used to confirm the formation of GOx/CNT. To investigate the catalytic activity and the optimal loading of GOx, its peak current and electron transfer rate constant, k<SUB>s</SUB>, are measured. In both ways, 2 mg mL<SUP>-1</SUP> GOx shows best results and its k<SUB>s</SUB> is 1.14s<SUP>-1</SUP>. From the relationship between scan rate and peak current, it is also revealed that this structure is (i) controlled by surface reaction and (ii) quasi-reversible. Regarding redox reaction of GOx, peak potential is linearly varied with pH with slope of -51 mV/pH. The slope indicates a typical two-electron reaction that is a desirable reaction pathway. GOx-catalyzed glucose oxidation reaction (GOR) is also investigated by reacting different concentrations of glucose with GOx/CNT layer. Peak current for GOR linearly increases with glucose concentration, proving that increase in glucose concentration promotes GOR. Therefore, GOx/CNT leads to high sensitivity of glucose (53.5 μAmM<SUP>-1</SUP>cm<SUP>-2</SUP>). When it comes to long term stability, activity of GOx/CNT is measured and 86% of the activity is maintained even after two weeks, indicating that long term stability of GOx is excellent.
Genetic Variability in Proton Beam Induced Mutants of Dandelion (Taraxacum officinale)
Jai Hyunk Ryu,Hyun Su So,Chang-Hyu Bae 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Ionizing radiations have been effective mutagen to overcome the limitation of the useful genetic resources in natural environment. This study was conducted to investigate an effect of proton beam on germination, growth patterns in the irradiated dandelion (Taraxacum officinale), and genetic variation in 12 morphological mutants induced in proton-beam irradiated dandelion. Percentage germination rate was drastically decreased over 250Gy. The lethal dose 50 (LD50) of germination was estimated between 250 Gy to 500 Gy. Significant decreases in growth patterns (plant height, number of leaf and fresh weight) were observed by increase of dose (Gy) of proton beam irradiation. According to the correlation analysis between dosage and growth factors, the orders of compactness of correlation were germination, plant height, fresh weight and number of leaf, respectively. Twelve morphological variants such as, dwarf, color, plastid, growth and leaf shape were screened at 50 to 250 Gy of the beam irradiation. As a result of ISSR analysis of the 12 variants, out of 33 bands detected overall, 8 bands were identified to be polymorphic with a rate of 24.2% at the control group. While 33 bands detected overall, 21 bands were identified to be polymorphic with a rate of 63.6% at the proton beam irradiation. The result indicates that the dandelion with proton beam treatment might be promoted variation at DNA level
한국 동물의 중독성 질병 발생상황 (1974년~2013년 6월)
이현경(Hyunk Young Lee),배유찬(You Chan Bae),이보람(Bo Ram Lee),이경현(Kyung Hyun Lee),백강현(Kang Hyun Baek),이명헌(Myoung Heon Lee) 대한수의학회 2013 大韓獸醫學會誌 Vol.53 No.3
Animal poisoning has been occurred in Korea. However, the lack of the data about animal poisoning in Korea makes clinicians and diagnostician difficult to obtain information on poisoning cases. In this paper, we tried to gather information about animal poisoning from 1974 to June 2013 in Korea. Animal and Plant Quarantine Agency (QIA) record database were used to examine recent trends in animal poisoning. The analysis showed that the cattle was reported to be the most common species involved in animal poisoning and botulinum toxin constituted the primary group of toxicants. Animal poisoning occurred frequently on January and in Gyenggi-do. Although the data present in this manuscript is a little, it will be helpful to understand the general trend of animal poisoning in Korea.
( Jai Hyunk Ryu ),( Bo Keun Ha ),( Dong Sub Kim ),( Jin Baek Kim ),( Sang Hoon Kim ),( Joon Woo Ahn ),( Il Yun Jeong ),( Han Jik Jo ),( Ee Yup Kim ),( Si Yong Kang ) 한국육종학회 2014 Plant Breeding and Biotechnology Vol.2 No.4
This study was carried out to evaluate the genetic diversity and relationships among fifty-six blackberry (Rubus fructicosus) mutants derived from gamma-ray treatment (fifty-two lines) by analysis of Amplified fragment length polymorphism (AFLP) markers. Both cluster analysis and principal coordinate analysis (PCOORDA) were conducted in order to study the genetic diversity, using both morphological traits and AFLP makers. A total of 589 bands were amplified with an average of 58.9 bands per primer. Among them, 560 were identified to be polymorphic, with a rate of 95.08%. A showed a highly significant (P≤0.01) positive correlation with GD and PIC (r2=0.999). MI also showed a significant (P≤0.05) positive correlation with GD and PIC. According to the clustering analysis, all mutant lines could be classified into five categories, but the three gamma-ray treatment lines and the cross-bred line were not clustered into any groups. For the morphological traits, cluster analysis divided the blackberry germplasm into six clusters and two independent groups. In addition, the morphological dendrogram indicated an unclear pattern of division among the groups based on AFLP analysis. The findings of this study indicate that mutant lines have high genetic diversity, and can be effectively utilized as materials for the improvement of breeding.
Jai Hyunk Ryu,Kyoungsun Seo,Hyun Su So,Sheong Chun Lee,Chang-Hyu Bae 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Dandelion has been widely used as a folkloric medicine for treatment of diverse diseases. This study was conducted to evaluate the effect of light spectrum using red (660 nm), blue (460 nm), red and blue mixed (Red : Blue = 6 : 4) LED (light emitting diodes) and fluorescent lamp on growth and functional components of dandelion (Taraxacum officinale). When LED was illuminated to T. officinale cv. Goldenboll, seed germination was delayed, and germination rate was the highest in the control (fluorescent light). The growth (plant height, root length and fresh weight) except leaf number was increased under the LED treatments compared with the control, and the growth promotion was the most effective in the red LED illumination. Total polyphenol contents in dandelion irradiated with the red and blue mixed or the red LED were 121.77 mg% or 115.36 mg%, respectively, which were greater than those in dandelion treated with blue LED and fluorescent lamp. Asparagine, proline, serine, threonine, glutamic acid and arginine were the predominant amino acids in dandelion and total amino acid was the highest under the Red LED illumination. The results indicate that application of the red and the mixed LED illumination promote growth and increase functional components during cultivation of dandelion