이관용의 사상 발전과 현실 인식

이현희 ( Hyunhee Lee ) 연세대학교 국학연구원 2016 동방학지 Vol.174 No.-

Lee Gwan-Yong formed his own philosophy and worldview through his time studying abroad in Europe. In his PhD thesis, he considered willpower as an independent element from perception and emotions. He believed that Koreans under Japanese colonial rule could recognize the independent movement for the nation’s liberation through their willpower. Based on Kant’s philosophy, he interpreted all human activity as the will of god. He also described the willpower’s mechanism based on Kant. Lee tried to overcome the practical difficulties of colonization based on his philosophy. This optimistic vision made his position evolve to look at the identity of the nation positively. He argued that Koreans should accept Western civilization. After returning home, he regarded Socialism as a selectable alternative for the reform of Korean Society. This represented a rather big ideological transition, as he had criticized both capitalism and communism as materialisms during his studies in Switzerland. Lee formed a critical perspective of bourgeois democracy while visiting Soviet Russia and Europe as a Dong-A Ilbo correspondent. On the other hand, his real socialism analysis was not accurate: he only took note of the ideal aspects of real socialism. After his Soviet journey, he promoted solidarity between the capitalist camp and the communist camp in Korea through Shinganhoe activities. In 1928, he faced China’s Nationalist Revolution as a Chosun Ilbo correspondent. Eventually, Lee expected to reform society through youth and the development of the higher education system.

The pepper RING finger protein CaRING1 plays a role in abscisic acid signaling and drought tolerance

Hyunhee Joo,Woonhee Baek,Chanmi Park,Sung Chul Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Plants are constantly exposed to a variety of biotic and abiotic stresses, which include pathogens and conditions of high salinity, low temperature, and drought. Abscisic acid (ABA) is a major plant hormone involved in signal transduction pathways that mediate the defense response of plants to abiotic stress. Previously, we isolated Ring finger protein gene (CaRING1) frompepper(Capsicum annuum), which is associated with resistance to bacterial pathogens, accompanied by hypersensitive cell death. Here, we report a new function of the CaRING1 gene product in the ABA-mediated defense responses of plants to drought stress. The expression of the CaRING1 gene was induced in pepper leaves treated with ABA or exposed to drought or NaCl. CaRING1-overexpressing (OX) transgenic plants showed enhanced sensitivity to ABA during the seedling growth and establishment. Furthermore, these plants were more tolerant to drought stress than the wild-type plants because of enhanced stomatal closure and increased expression of stress-responsive genes. Together, these results suggest that the CaRING1 acts as positive factor for drought tolerance in Arabidopsis by modulating ABA-mediated stomatal closing and gene expression.

The CabZIP2 pepper pathogen-induced bzip transcription factor positive regulator of disease resistance by promoting PR protein induction

Hyunhee Joo,Woonhee Baek,Chanmi Park,Sung Chul Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

A pepper bZIP transcription factor gene, CabZIP2, was isolated from pepper leaves infected with an a virulent strain of Xanthomonas campestris pv. vesicatoria (Xcv). Transient expression analysis of the CabZIP2-GFP fusion protein in Nicotiana benthamiana revealed that the CabZIP2 protein is localized in the cytoplasm as well as the nucleus. The acidic domain in the N-terminal region of CabZIP2 that is fused to the GAL4 DNA-binding domain is required to activate the transcription of reporter genes in yeast. Transcription of CabZIP2 is induced in pepper plants inoculated with virulent or avirulent strains of Xcv. The CabZIP2 gene is also induced by defense-related hormones such as salicylic acid, methyl jasmonate, and ethylene. To elucidate the in vivo function of the CabZIP2 gene in plant defense, virus-induced gene silencing (VIGS) in pepper and overexpression in Arabidopsis were used. CabZIP2-silenced pepper plants were susceptible to infection by the virulent strain of Xcv, which was accompanied by reduced expression of defense-related genes such as CaBPR1 and CaAMP1. CabZIP2 overexpression (OX) in transgenic Arabidopsis plants conferred enhanced resistance to Pseudomonas syringae pv. tomato DC3000. Together, these results suggest that CabZIP2 is involved in bacterial disease resistance.

Highly sulfonated polymer-grafted graphene oxide composite membranes for proton exchange membrane fuel cells

Lee, Hyunhee,Han, Jusung,Kim, Kihyun,Kim, Junghwan,Kim, Eunki,Shin, Huiseob,Lee, Jong-Chan THE KOREAN SOCIETY OF INDUSTRIAL AND ENGINEERING 2019 JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY -S Vol.74 No.-

<P><B>Abstract</B></P> <P>Sulfonated poly(arylene ether sulfone) (SPAES) composite membranes were prepared using graphene oxide (GO) and sulfonated poly(arylene thioether sulfone)-grafted graphene oxide (SATS-GO) as fillers for proton exchange membrane fuel cell (PEMFC) applications. When GO and SATS-GO were mixed with SPAES to form the composite membranes, dimensional stability, chemical stability, and mechanical strength were improved. The composite membranes containing SATS-GO especially showed higher proton conductivity than the pristine SPAES and the composite membrane containing GO because of highly sulfonated polymer (SATS) introduced on GO surface. Furthermore, the sulfonic acid groups in SATS-GO can increase the compatibility between the filler and matrix polymer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> SPAES composite membranes were prepared using highly sulfonated polymer-grafted graphene oxide as a filler. </LI> <LI> The filler was synthesized by grafting highly sulfonated polymer on GO surface using a mild reaction condition. </LI> <LI> Outstanding compatibility between the filler and matrix polymer. </LI> <LI> Physicochemical stability of the composite membranes was highly improved. </LI> <LI> Excellent proton conductivity of the composite membranes. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Regulation of Hepatic Gluconeogenesis by an ER-Bound Transcription Factor, CREBH

Lee, Min-Woo,Chanda, Dipanjan,Yang, Jianqi,Oh, Hyunhee,Kim, Su Sung,Yoon, Young-Sil,Hong, Sungpyo,Park, Keun-Gyu,Lee, In-Kyu,Choi, Cheol Soo,Hanson, Richard W.,Choi, Hueng-Sik,Koo, Seung-Hoi Elsevier 2010 Cell metabolism Vol.11 No.4

<P><B>Summary</B></P><P>Endoplasmic reticulum (ER)-bound transcription factor families are shown to be involved in the control of various metabolic pathways. Here, we report a critical function of ER-bound transcription factor, CREBH, in the regulation of hepatic gluconeogenesis. Expression of CREBH is markedly induced by fasting or in the insulin-resistant state in rodents in a dexamethasone- and PGC-1α-dependent manner, which results in the accumulation of active nuclear form of CREBH (CREBH-N). Overexpression of constitutively active CREBH activates transcription of <I>PEPCK-C</I> or <I>G6Pase</I> by binding to its enhancer site that is distinct from the well-characterized CREB/CRTC2 regulatory sequences in vivo. Of interest, knockdown of CREBH in liver significantly reduces blood glucose levels without altering expression of genes involved in the ER stress signaling cascades in mice. These data suggest a crucial role for CREBH in the regulation of hepatic glucose metabolism in mammals.</P> <P><B>Highlights</B></P><P>► PGC-1α/GR activates CREBH expression under fasting or insulin-resistant conditions ► CREBH enhances hepatic gluconeogenesis via a CRTC2-dependent manner ► Depletion of CREBH in the liver ameliorates fasting hyperglycemia in diabetic mice</P>

Exploring the Experiences of Automobile Manufacturing Workers Participating in Rehabilitation Exercise Programs after Industrial Accidents

Hyunhee Kim(Hyunhee Kim),Jaebum Lee(Jaebum Lee),Heejung We(Heejung We) J-INSTITUTE 2022 International Journal of Human & Disaster Vol.7 No.2

Purpose: This study was analyzed with a qualitative research method by applying a phenomenological method to examine the experiences and perceptions and attitudes about health care of workers participating in rehabil-itation exercises after suffering an industrial accident while working in the K automobile manufacturing industry in Korea. Method: As for the selection of research participants, a total of 12 participants were selected as the final research participants, including 1 female and 11 male, who could provide a wealth of information on the subject of the study. For data collection and analysis, Colaizzi's six-step analysis method was used to identify the partic-ipants' experiences and cognitive changes who participated in the rehabilitation program. Also, the participants' facial expressions and actions were recorded so that they could be used as additional observational data and reflected in the results of further research. Results: As a result of the study, 5 categories, 13 topic collections, 32 topics, and 97 construct meanings were finally confirmed. Specifically, the importance of strengthening the intrinsic motivation of the participants was emphasized in order to continuously practice health promotion after an industrial accident in the automobile manufacturing industry. In addition, it appears that a method of self-awareness of the necessity of exercise, giving fun of the complex program, and preventing and managing it in daily life is needed. Conclusion: The contents derived from the results of this study represent the changing perception of workers toward continuous health promotion management and improvement after an industrial accident. Through the research results, it was an opportunity to confirm the changing perception of health care among workers, and it is expected to be actively used in the future by developing rehabilitation exercise programs at the workplace in the automobile industry.

Improved Cell Selectivity of Symmetric α-Helical Peptides Derived From Trp-Rich Antimicrobial Peptides

Hyunhee Lee,양성태,신송엽 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.9

Although Trp-rich antimicrobial peptides (TRAMPs) such as tritrpticin and indolicidin exert potent antimicrobial activity, they have several drawbacks in therapeutic application, such as low cell selectivity and strong hemolytic activity. Here, we designed α-helical TRAMPs with symmetric amino acid sequences based on pseudo-symmetric tritrpticin and indolicidin. Compared with the parental pseudo-symmetric TRAMPs, the symmetric α-helical TRAMPs were highly effective against Gram-positive bacteria, including clinically isolated methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium. The symmetric α-helical TRAMPs exhibited negligible or very low toxicity against mammalian cells, including human erythrocytes. The symmetric peptides caused a rapid collapse of bacterial membrane potential and destabilized negatively charged lipid membranes, indicating that they act on the bacterial cytoplasmic membrane as a primary mode of action. Taken together, these findings imply that symmetric TRAMPs with an α-helical structure offer significant potential for development as novel therapeutics to overcome the problem of antibiotic resistance in Gram-positive pathogens.

Expressed Sequence Tag Analysis of Antarctic Hairgrass Deschampsia antarctica from King George Island, Antarctica

Lee, Hyoungseok,Cho, Hyunhee,Kim, Ilchan,Yim, Jounghan,Lee, Hongkum,Lee, Yookyung Korean Society for Molecular Biology 2008 Molecules and cells Vol.25 No.2

Deschampsia antarctica is the only monocot that thrives in the tough conditions of the Antarctic region. It is an invaluable resource for the identification of genes associated with tolerance to various environmental pressures. In order to identify genes that are differentially regulated between greenhouse-grown and Antarctic field-grown plants, we initiated a detailed gene expression analysis. Antarctic plants were collected and greenhouse plants served as controls. Two different cDNA libraries were constructed with these plants. A total of 2,112 cDNA clones was sequenced and grouped into 1,199 unigene clusters consisting of 243 consensus and 956 singleton sequences. Using similarity searches against several public databases, we constructed a functional classification of the ESTs into categories such as genes related to responses to stimuli, as well as photosynthesis and metabolism. Real-time PCR analysis of various stress responsive genes revealed different patterns of regulation in the different environments, suggesting that these genes are involved in responses to specific environmental factors.

Intravoxel incoherent motion MRI for monitoring the therapeutic response of hepatocellular carcinoma to sorafenib treatment in mouse xenograft tumor models

Lee, Yedaun,Lee, Seung Soo,Cheong, Hyunhee,Lee, Chang Kyung,Kim, Namkug,Son, Woo-Chan,Hong, Seung Mo Acta Radiologica 2017 Acta radiologica Vol.58 No.9

Cytoplasmic molecular chaperones in Pseudomonas species

Hyunhee Kim,Seongjoon Moon,Soojeong Ham,Kihyun Lee,Ute Römling,Changhan Lee 한국미생물학회 2022 The journal of microbiology Vol.60 No.11

Pseudomonas is widespread in various environmental and host niches. To promote rejuvenation, cellular protein homeostasis must be finely tuned in response to diverse stresses, such as extremely high and low temperatures, oxidative stress, and desiccation, which can result in protein homeostasis imbalance. Molecular chaperones function as key components that aid protein folding and prevent protein denaturation. Pseudomonas, an ecologically important bacterial genus, includes human and plant pathogens as well as growth-promoting symbionts and species useful for bioremediation. In this review, we focus on protein quality control systems, particularly molecular chaperones, in ecologically diverse species of Pseudomonas, including the opportunistic human pathogen Pseudomonas aeruginosa, the plant pathogen Pseudomonas syringae, the soil species Pseudomonas putida, and the psychrophilic Pseudomonas antarctica.