연령이 다른 흰쥐에 Bromobenzene 투여가 간 손상에 미치는 영향

윤종국,한선일,윤형원 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCINE 1999 Journal of biomedical laboratory sciences Vol.5 No.2

연령이 다른 흰쥐에 있어서 bromobenzene에 의한 간 손상의 정도 차이와 이의 기전을 알아보기 위하여 5주령 및 10주령 흰쥐에 bromobenzene을 체중 kg당 400mg을 격일로 5회 전처치함과 동시에 bromobenzene 전처치 및 대조군에 bromobenzene을 다시 투여한 뒤 처치하여 다음과 같은 결과를 얻었다. Bromobenzene 전처치로 인한 간 무게, 혈청 alanine aminotransferase, xanthine oxidase 활성 및 간 조직중 과산화지질의 함량 증가율과 간 세포질의 단백질 감소율은 10주령군이 5주령군 보다 높았다. 이 결과는 10주령이 5주령 보다 bromobenzene 투여로 인한 간 손상이 심하게 나타남을 시사해 주고 있다. 한편, 간 조직 중 aniline hydroxylase 활성은 10주령에 있어서 bromobenzene 전처치군 및 대조군 모두 5주령 보다 높게 나타나는 반면 glutathione S-transferase 활성은 10주령이 5주령 보다 낮게 나타났다. 또한 이들 실험동물에 bromobenzene을 재 투여한 다음 경시별로 간 조직 중 glutathione 함량을 측정한 바 10주령이 5주령 보다 glutathione 감소율이 낮게 나타났다. 이상 실험결과를 종합해 볼 때 bromobenzene 투여시 10주령이 5주령 실험군 보다 간 손상이 심하게 나타났으며, 이는 생체내의 bromobenzene처리능력이 5주령 보다 10주령이 저하되기 때문에 나타난 결과라고 사료된다. To evaluate an effect of growth periods on the bromobenzene-induced liver damage, bromobenzene was administrated to 5-week-old rats and 10-week-old rats pretreated with bromobenzene 5 times every other day for 10 days and then the animals were sacrificed. The results were obtained as follows; The increasing rate of serum levels of alanine aminotransferase, xanthine oxidase activity, hepatic lipid peroxide contents, liver weight per body weight (%) and decreasing rate of hepatic contents of protein to each control group were higher in 10-week-old rats than 5-week-old rats by the pretreatment of bromobenzene. According to the above results, 10-week-old rats indicated more severe liver injury than 5-week-old those in case of bromobenzene pretreatment. On the other hand, hepatic aniline hydroxylase activity was more increased in 10-week-old rats than 5-week-old rats both in control and bromobnezene pretreated rats where as the reverse in hepatic glutathione S-transferase. In case of hepatic GSH determination at the intervals of 2, 4, 8, 24 hours throughout 24 hr after administration of single dose of bromobenzene to 5-week-old and 10-week-old rats both in control and bromobnezene pretreated, the rate of GSH utilization was lower in 10-week-old rats than 5-week-old rats. In conclusion, from the above experimental, it is deduce that the 10-week-old rats showed more severe liver injury than 5-week-old rats by the bromobenzene treatment because the disposal ability of bromobenzene in liver was lower in 10-week-old rats than 5-week-old rats.

생쥐 소장의 interstitial cells of Cajal에서 ATP-민감성 K^(+)통로를 통한 향도잡이 전류의 조절

박찬국,김만우,김형석,최석,전제열 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.1

Objectives: This study was performed to investigate whether ATP-sensitive K^(+) channels exist in interstitial cells of Cajal (ICC) of mouse small intestine and the physiological role of ATP-sensitive K^(+) channels in gastrointestinal motility. Methods: ICC were isolated from mouse small intestine and cultured for 2 days. Electrical activities of ICC were recorded using the whole-cell patch-clamp technique at 30°C. Results: ICC displayed rhythmic slow waves and spontaneous inward currents (pacemaker currents). Pinacidil, an ATP-sensitive K^(+) channel opener, induced membrane hyperpolarization and decreased amplitude of slow waves in current clamping mode. Also, pinacidil inhibited amplitude and frequency of pacemaker currents and activated outward currents at a holding potential of -70 mV. Pinacidil effects on slow waves and pacemaker currents were blocked by glibenclamide, a specific blocker of ATP-sensitive K^(+) channel. Forskolin, an activator of adenylate cyclase, induced membrane hyperpolarization and inhibited pacemaker currents like as pinacidil and blocked by glibenclamide. Conclusions: These results suggest that ATP-sensitive K^(+) channels exist in ICC and may be play an important role in regulating gastrointestinal motility through the modulation of pacemaker activities of ICC.

2차 시지연 모델을 이용한 PID 제어기의 동조

황형수,류영국,모영승 원광대학교 2001 論文集 Vol.26 No.-

제어계의 축소 모델을 구하는 방법을 이용하여 Wang등은 다양한 동특성을 갖는 공정들에 대하여 범용적으로 적용할 수 있는 PID 동조 알고리즘을 제안하였다[7]. 그러나 Wang이 제안한 방법은 공정모델에 영점이 존재하지 않는 경우와 Nyquist선도가 -π/2및 -π를 지나는 경우에는 잘 적용이 되지만 공정모델에 영점이 존재하는 경우에는 동조 성능이 만족스럽지 못하였다. 본 논문에서는 공정 모델에 영점이 존재할 경우 Pade 축소법을 응용함으로써 이를 개선한 방법을 제안하였다. 이 방법은 저차 및 고차, 크고 작은 지연시간, 단조롭거나 진동하는 응답등의 다양한 동특성을 갖는 공정들에 범용적으로 적용할 수 있으며, 시뮬레이션을 통하여 이 방법이 다양한 공정에 우수한 특성을 나타냄을 보였다. The PID tuning algorithm which can applied generally to processes with varies dynamic characteristics is proposed by Wang[7]. However, it can be applied well to process model without zeros and with ∠G(jw)=- π/2 and - π point in Nyquist curve, but it gives unsatisfactory tuning performance for processes with zeros and without ∠G(jw)=- π/2 and - π in Nyquist curve. In this paper, the method which improve it using Pade reduction method is proposed. Satisfactory responses can be expected for processes with various dynamics, including those with low or high order, small or large dead time, monotonic or oscillatory responses. Simulation examples are given to show the effectiveness and flexibility of the controller in handling processes of different characteristics.

랫드에 있어서 Bromobenzene의 격일 투여 시, 매일 투여한 경우와 간손상 정도의 비교

이상희,윤종국,조현국 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.2

Bromobenzene의 투여 간격에 따라 간손상이 어떠한 차이를 나타내는지를 검토하기 위하여 흰쥐에 체중 1kg 당 400mg의 bromobenzene을 복강으로 2일 및 1일 간격으로 각각 3회 투여한 다음 간손상을 병리조직학적, 간기능적 측면에서 검토한 결과 2일 간격으로 투여한 실험군에서 간손상이 경미하게 나타났다. 그리고 간조직 중 cytochrome P450 함량은 2일 간격으로 투여한 실험군에서는 대조군 보다 증가되는 경향을 보였으나 1일 간격으로 투여한 경우에는 대조군 보다 오히려 유의한 (p<0.01) 감소를 보였다. 간조직 중 대조군에 대한 glutathione 감소율과 glutathione S-transferase 활성 증가율은 2일 간격으로 bromobenzene을 투여한 군이 1일 간격으로 투여한 실험군 보다 높게 나타났다. 이상 실험 결과는 동일한 양과 회수로서 bromobenzene을 격일로 투여한 실험동물에 있어서 매일 투여한 경우 보다 간손상이 경미하였으며, 이는 bromobenzene의 대사율이 증가됨으로서 나타난 결과로 생각된다. To evaluate the effect of intervals of bromobenzene treatment on the liver damage, the bromobenzene(400 mg/kg, i.p.) was given to rats at either one day or two days interval at three times. All the experimental animals were sacrificed at 24 hours after the last injection. Liver morphological changes were observed under a light microscopic examination and liver functional changes were determined by the measurement of alaine aminotransferase (ALT) activity and hepatic malondialdehyde (MDA) content. The experimental to examine the cause of liver damage were cytochrome P450, glutathione (GSH) content and glutathione S-transferase(GST) activities. The results are summarized as follows; Based on the liver morphological and functional findings, the daily bromobenzene-treated rats (ED) showed the more severe liver damage than every other day bromobenzene-treated rats (EOD). The hepatic cytochrome p450 content was higher in EOD group than that in ED group. And the increasing rate of hepatic GST activity and decreasing rate of GSH content to the control were higher in EOD group than that in ED group. In conclusion, the treatment of bromobenzene intermittently to the rats may lead to more reduced liver injury compared with the continuously treated animals when both cases are treated with the same dose and frequency, and it may be caused by the enhancement of bromobenzene metabolism.

고능력 젖소 축군의 집단번식관리프로그램 적용에 의한 번식효율의 개선

노경수,김의형,남현욱,서국현,강현구,김일화 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

We investigated the effect of reproductive herd health program (RHHP) on the reproductive performance in high yielding dairy herds. Data collected from 205 lactations at Boeun County, Chungbuk from April 2001 to Dec. 2003 included postpartum reproductive and metabolic diseases, body condition score (BCS), reproductive performance and milking records. First we compared the reproductive performance of cows without RHHP (pre-RHHP group) and cows with RHHP (RHHP group). Intervals from calving to first service (99.6 vs. 81.8 days) and to conception (160.3 vs. 131.8 days) were shorter in the RHHP group than in the pre-RHHP group. Secondly, we determined the effect of milk yield (305 days) on the occurrence of postpartum diseases, BCS changes, and reproductive performance in cows under RHHP, The occurrence of metritis and metabolic diseases were greater in the over-10,000 kg group than in the under-l0,000 kg group. The over-10,000 kg group lost more body condition than the under-10,000 kg group from calving to month 3 postpartum. Nevertheless, reproductive performance did not differ between the two groups. These results indicate that reproductive performance can be improved by the application of RHHP in high yielding dairy herds and that reproductive performance in the cows under RHHP are not affected by high milk yielding.

고정층 액상흡착탑내의 합성흡착제/콜리딘계의 열역학적 흡착특성

이성식,김형준,이봉국,최정균 동아대학교 환경문제연구소 1998 硏究報告 Vol.21 No.2

Adsorption Equilibria and Kinetics of collidine in a aqueous solution onto the synthetic resin adsorbent were carried out in a fixed bed absorber at 278, 288, 298 anti 308K for various concentration ranges respectively. The effective intraparticle diffusivity, D_(e), based on the concentration difference in the liquid phase, was determined by comparing experimental and theoretical uptake curves. The ratio of the effective diffusivity to moleculer diffusivity, D_(e)´/DAB were independent of the amount adsorbed and temperature for synthetic resin/collidine system. The result supported the concept that migrated of inter-microparticle gaps predominates over effective intraparticle mass transfer within synthetic porous adsorbent. Amount adsorbed q_(e) was correlated Q_(st) = 15.35 + [(-4.14) - q_(e)] and E_(a) = 6.28 + [(-1.671 q_(e)] with heat of adsorption(Q_(st)) and activation energy(E_(a)).

Elastic Open Activator를 이용한 Ⅱ급 부정 교합의 치료효과

정규림,박영국,이현경 대한치과교정학회 1995 대한치과교정학회지 Vol.25 No.5

Elastic Open Activator(E.O.A)는 myodynamic activator의 일종으로서 장치의 아크릴 부위의 대폭적인 크기 감소로 환자가 거부감 없이 장시간 착용할 수 있는 장점을 가지며, 구강내에서 매우 느슨하게 장착되므로써 보다 적극적인 근기능 자극 효과를 나타내고, 구강 내외의 제 기능시 발휘되는 생리적인 힘을 효율적으로 치조골 및 악관절부에 전달시킨다. 특히 기능모체(functional matrix)를 효과적으로 차단하는 동시에 능동적으로 자극함으로써 많은 부정교함 증례에서 비발치에 의한 교정치료의 성공률을 높이는 것으로 사료된다. 이 연구는 혼합치열기 및 초기 영구치열기의 Ⅱ급 부정교합 환자에게 사용된 E.O.A의 치료효과를 구명하기 위하여 시행되었으며 경희의료원 교정과에 내원하여 Ⅱ급 1류 및 2류 부정교합으로 진단되었던 각각 9명과 5명의 치료 전후 cephalogram 및 석고모형을 비교분석한 후 결과를 평가하여 다음과 같은 결론을 얻었다. 1. 상악골은 두 군 모두에서 정상적인 성장 양태를 유지하였다. 2. 하악골은 두 군 모두에서 크게 전하방 성장되었다. 3. 상악 전치는 Ⅱ급 1류 부정교합자에서는 설측, Ⅱ급 2류 부정교합자에서는 순측 경사되었고 두 군 모두에서 상하악 전치부 치조골 수직 성장이 억제되었다. 4. 하악 전치는 두 군 모두에서 약간 순측 경사되었다. 5. 두 군 모두에서 상, 하악 치궁의 확장이 일어났으며 Ⅱ급 2류 부정교합자에서 가용 공극의 길이가 증가되었다. The elastic open activator is one of the modified myodynamic activator. The reduced size of the appliance mass motivates the patients' comfort and longer time of wearing. Its peculiarities in loose fitting and the lack of appliance stabilization in the mouth draws the tongue and the surrounding functional matrices on close interaction with the appliance, consigns the physiologic exertion to target structures, and eventually makes it feasible to the inland of non-extraction treatment. In the context of the sagittal malocclusion, the orthodontic trench is dependent upon the growth of basal structure aimed, therefore, it is contemplated to grabble the effects of Elastic Open Activator upon the class Ⅱ malocclusion of growing child retrospectively. The cephalometric headfilms and study models of nine class Ⅱ malocclusion of growing child retrospectively. The cephalometric headfilms and study models of class Ⅱ division 1 and five division 2 patients were evaluated and analyzed, and the following observations were drawn ; 1. The maxilla maintained a normal growth pattern in both groups. 2. The mandible grew anteroinferiorly in both groups. 3. The upper incisors tipped ligually in Class Ⅱ division 1 and tipped labially in Class Ⅱ division 2 and anterior vertical alveolar growth was interrupted in both groups. 4. The lower incisors tipped labially. 5. There was an arch expansion in both groups and increase of available space in Class Ⅱ division 2

초기 성공적인 심폐소생술후 혈청 Interleukin-6 농도의 발현양상

박규남,박승현,황두영,김형국,이환,최경호,이원재,정욱성,김학주,김세경 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.4

Objective : To investgate patterns of serial serum interleukin-6(IL-6) concentrations after initial successful cardiopulmonary resuscitation, and to access differences in serum IL-6 concentrations in patients with Cerebral Performance Category(CPC, 1-2) and CPC(3-5) at the 5th day after cardiacpulmonary resuscitation(CPR). Methods : We studied prospectively 12 patients with spontaneous circulation after cardiopulmonary resuscitation. Venous blood samples were taken 2, 4, 12, 24, 48 and 72 hours after restoration of spontaneous circulation to measure serum IL-6 levels by the enzyme-linked immunosorbent assay. Results : Serum IL-6 concentrations in the severe neurolgically disabled group(CPC, 3-5) were significantly increased at 2, 24 and 48 hours after restoration of spontaneous circulation compared with the less neurolgically disabled group. Also, serum IL-6 concentrations in the severe neurolgic disabled group showed dual peak at 4 and 24 hours. Conclusion : Our study demonstrates significant increase of serum IL-6 in patients with CPC(3-5) after successful cardiopulmonary resuscitation and the significant correlation between early increase of serum IL-6 and poor neurologic outcome. It suggests that early increase of serum IL-6 can be used as a prognostic marker after successful cardiopulmonary resuscitation.

Host preference and development of Spodoptera frugiperda (Smith) on different crops (Lepidoptera: Noctuidae)

Hyung Cheol Moon,Su Ji Jang,Min Kyung Choi,Jang Ho Lee,Hyung Guk Kim,Min Sil Ahn 한국응용곤충학회 2022 한국응용곤충학회 학술대회논문집 Vol.2022 No.10

모델링을 고려한 RAD 도구의 설계

김형국(Hyung Guk Kim),주예찬(Yae Chan Ju),이민규(Min Kyu Lee),김명호(Myung Ho Kim) 한국정보과학회 1998 한국정보과학회 학술발표논문집 Vol.25 No.2Ⅰ

소프트웨어의 생명 주기가 급격히 짧아짐에 따라 생산성을 향상시키면서 개발 기간과 비용을 단축시킬 수 있는 RAD 도구가 최근 많이 유행하고 있다. 그러나 기존의 RAD 도구는 소프트웨어의 설계보다는 주로 사용자 인터페이스의 설계에 중점을 두고 있고, 소프트웨어의 설계를 위한 기존의 전문 소프트웨어 모델링 도구들은 규모가 크고 사용이 힘들 뿐 아니라 RAD 도구와의 직접적인 연관성을 제공하지 못하고 있다. 본 논문에서는 JAVA 언어를 위한 RAD 도구와 모델링 도구가 서로 유기적으로 결합된 새로운 RAD 도구를 구현하였다.