굴 효소 가수분해물의 angiotensin converting enzyme 저해작용

Hyung Joo Do(도형주),Hye Jin Park(박혜진),Ok Ju Kim(김옥주),Andre Kim(김안드레),Yeung Joon Choi(최영준),Se Young Choung(정세영),Jong-Myung Ha(하종명) 한국생명과학회 2012 생명과학회지 Vol.22 No.2

굴 효소 가수분해물은 굴을 alcalase, protamex, neutrase, flavourzyme, pepsin으로 각각 처리하였고, 이들의 ACE 저해활성을 측정하였다. ACE 저해활성은 가수분해 시간에 따라 증가 또는 감소하였으며, 그 중 10시간 이상 가수분해 처리한 PEH에서 가장 높은 ACE 저해활성을 나타내었다. PEH 가수분해물을 한외여과(30, 10 kDa)하여 낮은 분자량의 분획물을 얻었다. 30 kDa와 10 kDa 이하 분자량 별 ACE 저해활성은 각각 69.18±0.75, 83.71±1.12의 활성을 나타내었다. 이 결과 10 kDa 이하의 시료로부터 HPLC column (watchers 120 ODS-AP 250 X 4.6 (5 ㎛))을 이용하여 각각의 활성 분획을 분취하여 얻은 6분획의 ACE 저해 활성은 29.56~85.85% 로 나타났다. 그 중 저해활성이 가장 높게 나타나는 B fraction의 아미노산 서열을 확인한 결과 Leu-Gln-Pro 임을 확인하였고, peptide합성하여 얻은 저해활성 농도(IC??)가 1.18 uM임을 확인하였다. 이러한 결과는 PEH를 이용하여 건강 기능성 식품의 개발에 도움이 될 수 있을 것으로 생각된다. The peptides of enzymatic hydrolysates from oyster were determined by inhibitory activity against angiotensin-converting enzyme. The ACE inhibitory activity of enzymatic oyster hydrolysates increases with hydrolysis time. Among enzymatic oyster hydrolysates, oyster hydrolysates incubated with Protamex showed the best ACE inhibitory activity after 10 h. Hydrolysates were filtered through a HiSep ultrafiltration membrane (M.W. cut-off 30 kDa, 10 kDa) to obtain the peptide fractions with ACE inhibition activity. These fractions were applied to an HPLC column (watchers 120 ODS-AP 250 X 4.6 (5 ㎛)). Six active fractions were collected and the range of ACE inhibition was from 29.56 to 85.85%. Peptide was purified from fraction B, showing the highest ACE inhibitory activity, and its sequence was Leu-Gln-Pro. These results suggest that PEH may be beneficial for developing antihypertensive food and drug.

타크린으로 유발한 간세포 독성에 대한 효소별 굴 가수분해물의 보호 효과

Hye Jin Park(박혜진),Hyung Joo Do(도형주),Ok Ju Kim(김옥주),Andre Kim(김안드레),Jong-Myung Ha(하종명) 한국생명과학회 2012 생명과학회지 Vol.22 No.1

본 연구는 굴 가수분해물의 간 보호 효과를 확인하는 것을 목표로 하였다. 굴은 많은 기능적 요소를 가지고 있다고 알려져 있으며 특히, 효소에 의해 생산된 가수분해물은 우수한 기능적 물질을 포함한다. 타크린으로 유발한 간세포 독성에 대한 효소별 굴 가수분해물의 보호 효과를 확인하기 위하여 HepG2세포를 이용하여 in vitro상에서 확인하였다. 사용한 샘플은 Neutrase, Flavourzyme, Protamex을 이용하여 효소 가수분해한 것이다. 타크린으로 손상을 유발한 간세포에서는 GOT와 LDH가 증가하게 된다. 굴 효소 가수분해물을 처리한 실험군에서는 아무런 처리를 하지 않은 실험군에 비하여 높은 세포 생존율을 확인할 수 있었다. 또한 GOT와 LDH 역시 감소하는 것을 알 수 있었다. 본 연구를 통하여 타크린 독성에 대한 신약, 식품의 기초 자료를 제공할 수 있을 것으로 기대된다. This study investigated the potential hepatoprotective benefits of Crassostrea gigas oyster hydrolysates. Oysters are known to have many biofunctional properties. In particular, oyster enzymatic hydrolysates produce substances with beneficial functions. The potential hepatoprotective effects of C. gigas hydrolysates against damage induced by tacrine were evaluated in vitro in HepG2 cells. Peptides were generated from C. gigas by enzymatic hydrolysis with Neutrase, Flavourzyme, or Protamex enzyme preparations. Tacrine treatment induced considerable cell damage in HepG2 cells, as shown by significant leakage of glutamic oxaloacetic transaminase (GOT) and lactate dehydrogenase (LDH). Cells treated with C. gigas hydrolysates showed an increased resistance to oxidative challenge compared to control cells, as revealed by higher cell survival against tacrine-induced hepatotoxicity. In addition, treatment with C. gigas hydrolysates reduced the leakage of GOT and LDH. These findings indicate that enzyme hydrolysates derived from C. gigas may be of benefit for developing hepatoprotective foods and drugs.

증령적 변화로서의 두개골 봉합의 유합 상태

안형준,신경진,권정승,도형주,최종훈,김종열,Ahn, Hyoung-Joon,Sin, Kyoung-Jin,Kwon, Jeong-Seung,Do, Hyung-Joo,Choi, Jong-Hoon,Kim, Chong-Youl 대한안면통증구강내과학회 2001 Journal of Oral Medicine and Pain Vol.26 No.4

In order to study the closure stage of cranial sutures and its correlations with age, the ectocranial closure stage of coronal suture, sagittal suture, and lambdoidal suture of 67 skulls was measured. Among the skulls kept at the department of anatomy, college of medicine, Yonsei University, the ones with ages identified were used for this study. These measurements of suture closure were conducted by 4 examiners independently. The sutures were further divided by Frederic's method into 16 suture parts. The closure stages were classified by five stages of Broca-Ribbe. The following results were obtained: 1. The inter-observer reliability among 4 examiners showed high intraclass correlation coefficient of over 0.75(mean : 0.856) in all suture parts. Therefore, the determination of closure stage wasn't influenced by the subjective view of each examiner. 2. In all suture parts, the closure stage increased proportionally with age.(p<0.01) In terms of each suture part, the S2 part of sagittal suture showed the highest correlation(68.1%) while the L1-R part of lambdoidal suture showed the lowest correlation(51.3%). In addition, in terms of suture types, the correlation with age decreased in the order of sagittal suture(60.0%), coronal suture(57.7%), and lambdoidal suture (55.7%). In general, the average value of suture closure stages had 57.8% correlation with age(p<0.01). 3. The most frequent suture closure stage according to age group was '0' for ages below 30, '0' and '1' for ages within the 30's, '1' and '2' for ages within the 40's, and '2' for ages within the 50's. With older age groups, the frequency of '3' and '4' increased, and the suture closure stage increased proportionally with age. 4. The mean age by closure stage of each suture were within the 40's for the closure stage of '1', within the 50's for the closure stage of '2', and from 50's through 60's for the closure stage of '3'. The standard deviation was over 10 for all closure stages. In addition, at the same suture closure stage, the mean age according to the coronal suture was higher than the ages according to the sagittal suture or lambdoidal suture. Especially, C1-R, C1-L, C2-R, and C2-L parts showed the highest age when at the same suture closure stage. 5. The values appropriate for age estimations using suture closure stages of 16 suture parts were calculated, and a calculator for age estimation ($R^2=0.6944$, p<0.01) by ectocranial suture closure stage for Koreans is presented. From the above results, the method of using the closure stage of sutures of the skull to estimate age can be useful in individual identification of forensic science. Further extensive and accurate research using larger samples would be worthy of study.

두힘살근 앞힘살의 형태변이와 두힘살근 뒤힘살과 붓목뿔근 사이의 위치 관계

김다혜(Da-Hye Kim),도형주(Hyung-Joo Do),김현주(Hyun-Joo Kim),원성윤(Sung-Yoon Won),최다예(Da-Yae Choi),허경석(Kyung-Seok Hu),최종훈(Jong-Hoon Choi),김희진(Hee-Jin Kim) 대한체질인류학회 2010 해부·생물인류학 (Anat Biol Anthropol) Vol.23 No.1

간추림 : 본 연구는 한국인 두힘살근 앞힘살의 형태학적 분석과 더불어 두힘살근 뒤힘살과 근접하며 동일한 신경분포를 갖는 붓목뿔근(stylohyoid muscle)과 두힘살근 뒤힘살 사이의 해부학적 위치 관계를 확인하고자 하였다. 본 연구의 재료로는 한국 성인시신 34구(남: 21, 여: 13, 평균나이: 65 (24~89세))를 사용하였다. 34구의 시신 중 8예에서 두힘살근 앞힘살과 턱목뿔근의 덧힘살 (accessory belly)을 확인하였다. 덧힘살은 아래턱솔기(mandibular raphe)의 표면을 교차하지 않는 유형(unilateral type)과 교차하는 유형(crossover type)으로 분류하였으며, 각각 3예와 5예에서 나타났다. 두힘살근 뒤힘살과 붓목뿔근 사이의 국소적인 위치 관계를 관찰한 결과, 관찰한 49쪽 중 32쪽(65%)에서 붓목뿔근의 힘살이 안쪽과 가쪽으로 나뉘어 두힘살근 뒤힘살의 힘줄을 감싸면서 목뿔뼈로 닿았으며, 이를 붓목뿔근 힘살이 두힘살근 뒤힘살 힘줄의 가쪽으로 더 두꺼운 경우(21쪽)와 안쪽이 가쪽 부분보다 더 두꺼운 경우로 세분하였다 (11쪽). 관찰한 49쪽 중 17쪽(35%)에서 붓목뿔근이 두힘살근 뒤힘살보다 안쪽으로 지났으며, 붓목뿔근의 힘살이 두힘살근 뒤힘살의 가쪽으로 주행하는 경우는 관찰되지 않았다. 두힘살근 앞힘살 부위에 존재하는 덧힘살의 형태는 매우 다양하게 나타났으며, 턱끝밑부위의 이러한 불규칙적인 형태는 비심미적인 목의 외형을 형성할 뿐 아니라 임상에서의 진단과 수술 과정에 어려움을 줄 수 있다. 대부분의 자료에서는 붓목뿔근 힘줄의 끝부분을 두힘살근의 중간힘줄이 관통하는 것으로 묘사하고 있으나, 연구 결과 붓목뿔근이 두힘살근 뒤힘살의 안쪽으로 지나는 경우가 35%를 차지하였다. 본 연구는 두힘살근 부위의 해부학적 구조 관계에 대한 보다 정확한 이해와 더불어 턱끝밑부위(submental region)와 위목부위(upper neck region)의 수술을 포함한 치료 시 적용 가능한 지침을 제시할 수 있을 것으로 기대한다. The digastric muscle is located in the suprahyoid region which consists of anterior belly, intermediate tendon and posterior belly. This muscle is an important landmark when performing an operation of submental and upper neck region. Previous researches have reported about variations of the anterior and posterior belly of digastric muscle. However, there are few studies about the general morphology of the digastric muscle and the relationship with surrounding muscles. The purpose of this study was to analyze the morphology of the anterior belly of digastric muscle and confirm the topographic relationship between the digastric muscle and the stylohyoid muscle of Korean. Thirty-four cadavers (21 males, 13 females; mean age 65 years; range 24~89 years) were used in this study. The skin, subcutaneous tissues, superficial fascia and platysma were removed and a detailed dissection was performed, with extreme care being taken not to damage underlying the muscles of submental and upper neck region. After the dissections, all specimens were sketched and photographed. In 8 specimens, we observed the accessory bellies of the anterior belly of digastric muscle. We classified the accessory bellies into the crossover type (five specimens, 14.7%) that cross the mandibular raphe and unilateral type (three specimens, 8.82%). The findings resulting from observation of the anatomical relationship between the posterior belly of digastric and stylohyoid muscles, the posterior belly of digastric muscle perforated the stylohyoid muscle in 32 cases (65%) out of 49 sides. This case was subdivided into the belly of the stylohyoid muscle lean to the lateral (twenty-one specimens, 42.9%) or medial side (eleven specimens, 22.4%) of the posterior belly of digastric muscle. In 17 specimens (35%), the stylohyoid muscle existed on the medial side of the posterior belly of digastric muscle.

유전자조작, 균주분리 알긴산 분해 Methylobacterium sp. HJM27 균주의 분리 및 특성

김옥주 ( Ok Ju Kim ),이동근 ( Dong Geun Lee ),이성목 ( Sung Mok Lee ),이석준 ( Suck June Lee ),도형주 ( Hyung Joo Do ),박혜진 ( Hye Jin Park ),김안드레 ( Andre Kim ),이재화 ( Jae Hwa Lee ),하종명 ( Jong Myung Ha ) 한국미생물생명공학회 2010 한국미생물·생명공학회지 Vol.38 No.2