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Jo, Nam Hyun,Kim, Jung Young,El‐,Gamal, Mohammed I.,Choi, Won‐,Kyoung,Park, Jin‐,Hun,Kim, Eun Jung,Cho, Jung‐,Hyuck,Ha, Hyun‐,Joon,Choi, Tae Hyun,Oh, Chang‐,Hyun John Wiley Sons, Ltd. 2011 Journal of labelled compounds & radiopharmaceutica Vol.54 No.2
<P><B>Abstract</B></P><P>Synthesis, radiolabelling, and <I>in vitro</I> evaluation of a new <SUP>125</SUP>I‐labelled iodouracil hexitol nucleoside analogue are reported. The target compound was successfully synthesized by an iodination–destannylation method and then purified by reverse phase HPLC. The radiochemical purity of the product was >99% with decay‐corrected yields of 48±3%. <I>In vitro</I> cellular uptake testing was carried out using MCA and MCA‐tk cell lines for comparison of compound 1 with [<SUP>18</SUP>F]FHBG. The newly synthesized compound 1 showed higher accumulation in herpex simplex virus type 1 thymidine kinase (HSV1‐tk) gene expression cell line (MCA‐tk cell line) than in the wild type MCA cell line compared with [<SUP>18</SUP>F]FHBG. The MCA‐tk to MCA cellular uptake ratio for compound 1 was higher than that of [<SUP>18</SUP>F]FHBG from 2 h after incubation. The radioiodine‐labelled compound 1 (I‐125, <I>t</I><SUB>1/2</SUB>=59.37 days) has a longer physical half‐life than F‐18‐(<I>t</I><SUB>1/2</SUB>=110 min) labelled FHBG. Radioiodine‐labelled compound 1 could be used for monitoring gene expression for a long time. The selectivity for MCA‐tk cell line makes compound 1 a promising imaging agent for HSV1‐tk expression. Copyright © 2010 John Wiley & Sons, Ltd.</P>
Ischemic brain imaging using fluorescent gold nanoprobes sensitive to reactive oxygen species
Hyun, H.,Lee, K.,Min, K.H.,Jeon, P.,Kim, K.,Jeong, S.Y.,Kwon, I.C.,Park, T.G.,Lee, M. Elsevier Science Publishers 2013 Journal of controlled release Vol.170 No.3
Fluorescein-labeled hyaluronic acids (HA) were immobilized on gold nanoparticles for reactive oxygen species (ROS) detection. The efficacy of HA immobilized gold nanoparticles (HHAuNPs) was evaluated in a stroke animal model. The stroke rat model was produced by transient middle cerebral artery occlusion (MCAO), which induced transient ischemia and reperfusion (I/R) in the brain. The increase of ROS in the I/R brain was confirmed by TBARS assay with the brain extracts. For brain imaging, HHAuNPs were injected into the rat brain 1h before transient MCAO. Five hours after the injection, the rats were sacrificed and the brains were subjected to imaging analysis. The results showed that stronger signals were detected in the I/R brains than in the normal brains. To identify the time window for effective detection of ROS, HHAuNPs were injected into the post-ischemic rat brains at various time points. The results showed that ROS level reached a maximum at 24h after the transient MCAO. Also, a live imaging study was performed with HHAuNPs in the normal and I/R animals. The results confirmed that ROS level increased in the I/R animal group with time, while the signal was decreased in the normal animal group. Together, our results suggest that HHAuNPs may be useful to monitor ROS level in the ischemic brain and to identify the infarct areas in ischemic brains for the treatment of stroke.
Lee, Hyun-Wook,Verlander, Jill W,Handlogten, Mary E,Han, Ki-Hwan,Cooke, Paul S,Weiner, I David BioScientifica Ltd 2013 Reproduction Vol.146 No.3
<P>The rhesus glycoproteins, Rh B glycoprotein (RHBG) and Rh C glycoprotein (RHCG), are recently identified ammonia transporters. Rhcg expression is necessary for normal male fertility, but its specific cellular expression is unknown, and Rhbg has not been reported to be expressed in the male reproductive tract. This study sought to determine the specific cellular expression of Rhcg, to determine whether Rhbg is expressed in the male reproductive tract, and, if so, to determine which cells express Rhbg using real-time RT-PCR, immunoblot analysis, and immunohistochemistry. Both Rhbg and Rhcg were expressed throughout the male reproductive tract. In the testis, high levels of Rhbg were expressed in Leydig cells, and Rhcg was expressed in spermatids during the later stages of their maturation (steps 13–16) in stages I–VIII of the seminiferous epithelium cycle. In the epididymis, basolateral Rhbg was present in narrow cells in the initial segment, in principal cells in the upper corpus, and in clear cells throughout the epididymis. Apical Rhcg immunolabel was present in principal cells in the caput and upper corpus epididymidis and in clear cells in the middle and lower corpus and cauda epididymidis. In the vas deferens, apical Rhcg immunolabel and basolateral Rhbg immunolabel were present in some principal cells and colocalized with H<SUP>+</SUP>-ATPase immunolabel. We conclude that both Rhbg and Rhcg are highly expressed in specific cells in the male reproductive tract where they can contribute to multiple components of male fertility.</P>
Sivagami, I. Nirmal,Prasanna, K.,Santhoshkumar, P.,Jo, Yong Nam,Kang, Suk Hyun,Kim, Tae Hong,Lee, Chang Woo American Scientific Publishers 2017 Journal of nanoscience and nanotechnology Vol.17 No.11
<P>A novel lithium transition metal orthosilicate compound, i.e., lithium zinc silicate (Li2ZnSiO4), is investigated as a potential cathode material for lithium ion batteries(LIBs). Li2ZnSiO4 is successfully synthesized via a facile sol-gel technique followed by a calcination step. The studied physical and electrochemical properties substantiate the Li2ZnSiO4 compound to be considered as a potential positive electrode in LIBs. The X-ray diffraction (XRD) results reveal an orthorhombic structure of Li2ZnSiO4 with sharp crystalline peaks. Field emission transmission electron microscopy (FE-TEM) images show a plate-like structure of a single particle with clear lattice fringes. The mapping results of FE-TEM along with X-ray photoelectron spectroscopy (XPS) confirm the chemical composition of Li2ZnSiO4 crystals. Field emission scanning electron microscopy (FE-SEM) further confirms the FE-TEM results on the morphology of Li2ZnSiO4 with a plate-like structure and an average particle size of 5 mu m. Electrochemical impedance spectroscopy (EIS) studies shows an initial charge transfer resistance (R-ct) of 371 Omega. An initial discharge capacity of 133 mAh g(-1) is obtained at the C/10 rate, which is comparable to other orthosilicate compounds.</P>
( Cho I Lee ),( So Young Kwon ),( Jeong Han Kim ),( Won Hyeok Choe ),( Chang Hong Lee ),( Eileen L Yoon ),( Jong Eun Yeon ),( Kwan Soo Byun ),( Yun Soo Kim ),( Ju Hyun Kim ) The Editorial Office of Gut and Liver 2014 Gut and Liver Vol.8 No.1
Background/Aims: We investigated the efficacy and safety of tenofovir disoproxil fumarate (TDF)-based treatment in chronic hepatitis B (CHB) patients who failed previous antiviral therapies. Methods: Seventeen patients who failed to achieve virological responses during sequential antiviral treatments were included. The patients were treated with TDF monotherapy (four patients) or a combination of TDF and lamivudine (13 patients) for a median of 42 months. Hepatitis B virus (HBV) DNA and hepatitis B e antigen (HBeAg) were measured, and renal function was also monitored. Results: Prior to TDF therapy, 180 M, 204 I/V/S, 181 T/V, 236 T, and 184 L mutations were detected. After TDF therapy, the median HBV DNA level decreased from 4.6 log10 IU/mL to 2.0 log10 IU/mL and to 1.6 log10 IU/mL at 12 and 24 months, respectively. HBV DNA became undetectable (≤20 IU/mL) in 14.3%, 41.7%, and 100% of patients after 12, 24, and 48 months of treatment, respectively. HBeAg loss was observed in two patients. Viral breakthrough occurred in five patients who had skipped their medication. No significant changes in renal function were observed. Conclusions: TDF-based rescue treatment is effective in reducing HBV DNA levels and is safe for patients with CHB who failed prior antiviral treatments. Patients` adherence to medication is related to viral rebound. (Gut Liver 2014,8:64-69)
( Jin-sung Yuk ),( Won I. Park ),( Jung Hun Lee ),( Hyeong Sik Ahn ),( Hyun Jung Kim ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-
목적: The purpose of this study was to compare treatment success rates, side effects rates and time to resolution between single-dose protocol and non-single-dose protocol (multi-dose protocol and two-dose protocol) of methotrexate (MTX) in treatment of ectopic pregnancy (EP) using randomized controlled trials (RCTs). 방법: We searched MEDLINE, EMBASE, SCOPUS, Web of Science, the Cochrane Central Register of Controlled Trials, ClinicalTrials.gov, International Clinical Trials Registry Platform and EU Clinical Trials Register. The relative risk (RR) and 95% confidence intervals (CIs) were used for calculating dichotomous outcomes, while mean difference (MD) and 95% CIs were used for calculating continuous outcomes. 결과: We selected 9 RCTs among total 1521 articles. The success rate of single-dose protocol was similar to that of non-single-dose protocol in treating EP [RR (95% CI): 1.02 (0.98~1.06; 9 trials, 891 patients), I2=0%]. Although non-single-dose protocol tended to have a higher side effects compared to single-dose protocol, there was no significant difference in both group, [RR (95% CI): 0.76 (0.58~1.01; 7 trials, 704 patients), I2=12%]. However, in subgroup analysis about tubal pregnancy, non-single dose protocol appeared to have a higher side effects rate than single-dose protocol [RR (95% CI): 0.69 (0.51~0.93; 3 trials, 369 patients), I2=0%]. Single-dose protocol had longer resolution time than non-single-dose protocol [MD (95% CI): 4.5 (0.40~8.60; 5 trials, 528 patients), I2=83%]. 결론: Single-dose protocol has similar success rates to non-single-dose protocol in treating EP, and tends to lower side effects rate than non-single- dose protocol. Also, in treating tubal pregnancy, single-dose protocol has lower side effects rate than non-single-dose protocol. However, single- dose protocol has longer resolution time than non-single-dose protocol.
Continuous enrichment of circulating tumor cells using a microfluidic lateral flow filtration chip
Lee, S.W.,Hyun, K.A.,Kim, S.I.,Kang, J.Y.,Jung, H.I. Elsevier 2015 Journal of chromatography A Vol.1377 No.-
The isolation and characterization of circulating tumor cells (CTC) is of great importance in cancer diagnosis and prognosis. Highly sensitive detection of CTCs can be very difficult because they are extremely rare (i.e., 1-5 CTCs per 10<SUP>9</SUP> erythrocytes) in blood. Recently, various devices have been developed that exploit biochemical (affinity-based) and physical (size or density) methods. Antibody-based isolation has its own limitations, as the expression level of the epitopes for an antibody varies due to the heterogeneity of cancer cells. Harsh conditions associated with physical methods can cause the deformation and damage of CTCs during the isolation process. Here, we propose a microfluidic lateral flow filtration (μ-LaFF) chip in which lateral flow was combined with vertical flow into the filter to capture the CTCs gently. The CTCs experienced weak shear flow owing to the lateral flow and traveled alongside the filter channel until finally being captured. The vertical flow in the filter held the captured cells tightly and served as an exit for uncaptured hematological cells (white and red blood cells). From our μ-LaFF chip we obtained a high capture efficiency (95%) and purity (99%), minimizing any damage to the CTCs. Our μ-LaFF technology is expected to be useful in the diagnosis and prognosis of various cancers.
이재관,오민정,신중식,Lee Kyung Joo,Nam Jung Hyun,Cha Jung Hak,Chang Jin Dong,Cho Dong Hee,Kang In Soo,Lee Paul I 대한의학회 2005 Journal of Korean medical science Vol.20 No.3
We detected pregnancy related new molecule, human chorionic gonadotropin related protein (hCGRP) in the urine of a pregnant women by using a monoclonal antibody against the human chorionic gonadotropin (hCG). This study examined the effectiveness of urinary hCGRP quantification in diagnosing ectopic pregnancy. This study included 40 normal pregnant women and 25 patients with ectopic pregnancy. Patients’ serum and urinary intact whole hCG (i-hCG) and hCGRP concentrations were measured using sandwich ELISA and the ratio of hCGRP to i-hCG was calculated. Statistical analysis was performed using statistical package for social sciences (SPSS) 10.0. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the cut-off value to discriminate ectopic pregnancies from normal intrauterine pregnancies. Urinary hCGRP and hCGRP/i-hCG ratio in ectopic pregnancy group (14±6.6 ng/mL, 4.6±1.9%, respectively) were significantly lower than those of normal pregnancy group (149±10.2 ng/mL, 29.7±1.9%, respectively; p<0.001). Based on ROC curve analysis, a cut-off point of urinary hCGRP/i-hCG ratio <16.2% discriminated between ectopic pregnancy and normal pregnancy with a sensitivity, specificity, positive predictive value and negative predictive value of 92.0%, 90.0%, 32.6%, and 99.5%, respectively. Urinary hCGRP/i-hCG ratio measurement may be effective in diagnosing ectopic pregnancy.
이현철,유호종,김진섭,함성호,신장규,이종현,이정희,권성원,박세일 경북대학교 센서기술연구소 1997 센서技術學術大會論文集 Vol.8 No.1
A planar Bi-Sb multijunction thermal converter for the precise measurements of ac voltage and current has been fabricated and its characteristics was discussed. In order to convert ac power into heat, a bifilar thin film Pt-heater, which could cancel its Thomson and Peltier effects, was prepared on the Si_(3)N_(4)/SiO_(2)/Si_(3)N_(4) diaphragm for the thermal isolation between heater and silicon substrate. To convert the temperature or the heat generated by the heater into dc voltage and current, hot and cold junctions of the Bi-Sb thermopile, which has a large difference in Seebeck coefficients, were formed on the dielectric diaphragm and the silicon substrate, respectively. The respective thermal sensitivity of the thermal converter with a bifilar heater was about 10.1 mV/mW and 14.8 mV/mW in the air and vacuum, which is about eight times higher than that of commercial 3-dimensional thermal converter. In the case of ac 2 V-input voltage, the ac-dc voltage transfer difference was about ±2.0 ppm, and in the case of ac 10 mA-input current, the ac-dc current transfer difference was about ± 0.6 ppm, in the frequency range from 10 Hz to 10 kHz.