Interleukin-1으로 유도된 흰쥐의 급성 폐손상에서 phospholipase A_2의 활성증가

조현국,문병연 경운대학교 산업기술연구소 2002 産業技術硏究論文誌 Vol.5 No.1(B)

In order to investigate the pathogenesis of adult respiratory distress syndrome (ARDS), acute lung injury was induced in rats by insufflation of interleukin-1α (IL-1, 50 ㎍/rat) into the trachea. To identify acute edematous lung injury, five hours after IL-1 administration, lung leak index, lung lavage phospholipid content, and lung lavage protein concentrations were measured. In addition, activities of lung myeloperoxidase (MPO) and phospholipase A_2 (PLA_2), and immunocytochemical changes were examined. IL-1 increased lung leak index, phospholipid content in bronchoalveolar lavage (BAL) fluid, and BAL protein concentrations. Lung PLA_2 activity was significantly increased by IL-1 administration, compared with control. In an immunocytochemical study, PLA_2 antiserum particles were identified in alveolar type Ⅱ epithelial cytoplasm in rats given IL-1 intratracheally. Based on these experimental results, it is suggested that increased PLA_2 activity induced neutrophil-dependent oxidative lung injury and the oxidative lung injury was caused by proinflammatory lipid mediators produced by PLA_2.

노화 흰쥐 간에 미치는 비타민 A 급성과다 효과 ㅣ.조직학적 연구와 Alkaline phosphatase 활성

조현국,박원학,정형재 嶺南大學校 基礎科學硏究所 1993 基礎科學硏究 Vol.13 No.-

급성 비타민 A 과다가 노화과정의 진행정도(3, 6, 20개월)에 따라 간장에 미치는 영향을 형태학 및 효소화학적으로 관찰하였다. 대조군에 대한 광학현미경적 변화로 3, 6개월군은 대체로 그 형태가 잘 보존되어 있으나 20개월군에서는 동양혈관의 확장, 불규칙한 간세포삭, 국소적 세포팽창과 염증세포의 침윤이 관찰하였다. 비타민 A 투여 3개월군에서의 광학현미경적 변화로, 간소엽의 주변부변성이 관찰되었으며, 6개월군에서는 중심정맥과 간정맥 내막의 섬유증식, 그리고 섬유모세포와 성상세포의 중식이 국소적으로 형성되었다. 20개월군에서는 소엽 전반에 걸친 동양혈관의 확장, 간정맥 경계부의 섬유중식, 중심정맥과 동양혈관의 심한 섬유증식에 기인된 동양혈관 내막에서의 적혈구 정체 및 세포질의 소실도 관찰되었다. 효소활성의 변화로, 혈액중의 ALT, AST는 변화가 없는 반면, ALP 활성은 비타민 A 투여 20개월군에서 증가하는 경향을 보였다. The effect of acute hypervitaminosis A on liver tissue, obtained from 3,6, 20months of rats, were studied by analyzing morphological changes and serum enzyme activities. In light microscopic results of control groups(3,6 months), the tissue structure is preserved well, whereas from 20 months old group, dilatation of sinosoids, irregular hepatic cell cord, and focal cell swelling and infiltration of inflammatory cell were observed. From the 3 months groups treated with vitamin A, peripheral degeneration in the hepatic lobule was observed. From the 6 months groups, fibrosis of central and portal veins, and formation of focal proferation of fibroblasts and Kupffer cells. The dilatation of sinusoids in the whole liver tissue, fibrosis of continuity of liver tissue in adjacent lobule, and focal stagnated red blood cells(caused from the fibrosis of central vein and sinusoids) with cytoplasmic loss were observed from 20 months group. The activity of serum ALP level in 20 months group treated with vitamin A, compared with 3, 6 months groups, was increased, while the changes of serum ALT and ASt activities were insignificant.

土川芎과 日川芎의 效能 및 品質比較에 關한 硏究 (2) : 두 유형의 천궁과 천궁 - 당귀 배합시의 혈관 이완효능 Vascular Relaxant Effect of Cnidii rhizoma and Cnidii rhizoma - Angelicae radix Compound

李項雨,趙顯國,朴涌基 대한본초학회 1999 大韓本草學會誌 Vol.14 No.1

In order to investigate the vascular relaxant effect of Ligusticum chuanxiong, Cnidium officinale and Angelicae radix mixed with each of them, we examined with physiography using thoracic aorta of rats. Samples were prepared to be divided Ligusticum chuanxiong (TC), Cnidium officinale (IC), TC removed oil (TCRO), oil extracts from TC (TCO) and IC (ICO), and Angelicae radix (D) mixed with TC (TC/D), IC (IC/D), and TCRO (TCRO/D). From norepinephrine and phenylephrine induced vascular contraction, all the samples had relaxant effect. IC was more effective vascular relaxant capacity compared to TC, however, as removed oil from TC (TCRO) the relaxant capacity was markedly elevated and higher than that of IC. Mixing the Angelicae radix, relaxant effect between TC/D and IC/D was that TC/D was more effective vascular relaxant capacity than that of IC/D. Whereas there was not relaxant effect in TCO and ICO, and TCRO/D was declined in relaxant rotio compared to TCRO. Based on these results, it is suggested that Angelicae radix is very effective material to elevate the vasodilation effect of Ligusticum chuanxiong and Cnidium officinale.

노화 흰쥐 간에 미치는 비타민 A 급성과다 효과 : Ⅱ.미세구조적 연구 Ⅱ.Ultrastructural Study

정형재,박원학,조현국 嶺南大學校 基礎科學硏究所 1994 基礎科學硏究 Vol.14 No.-

급성 비타민 A 과다가 노화과정의 진행정도(3,6,20개월)에 따라 간조직에 미치는 영향을 미세구조적으로 관찰하였다. 대조군 6개월의 세포구조는 불규칙한 핵막, 담모세관 주위의 dense body 증가와 더불어 RER의 종창이 관찰되었다. 20개월군에서 미토콘드리아의 소실, hyaloplasm의 형성 뿐만 아니라 residual body의 수적 증가가 나타났다. 비타민 A 투여군은 간정맥부와 동양혈관 변연부의 섬유화, 세포질 수포현상, 그리고 노화진행에 따라 세포간질의 협착이 보였다. 3,6개월군의 간세포질에서는 미토콘드리아의 소실과 RER의 붕괴가 관찰되었을 뿐만 아니라 지질저장 세포내의 지방소적의 축적과 담모세관 주위의 dense body 증가가수반되었다. 더우기 지질저장세포내의 지방소적은 그 축적도에 있어서 20개월군에서 증가하였다. 특히 간세포질에서 섬유가 관찰되였다. The effects of acute hypervitaminosis A on liver tissue, obtained from 3, 6, 20 months of rats, were studied by observing ultrastructural changes. The celluar structure in control grorp of 6 minths showed irregular nuclear envelope, dense bodies around bile canaliculi, and swelling of endoplasmic reticulum. That of 20 months appeared not only a loss of mitochondria, and formation of hyaloplasm, but also the number of residual body was increased. The results from treated with vitamin A, showed fibrosis of portal vein, cytoplasmic bullae, perisinusoidal fibrosis, and occlusions of intercellular space dependent upon aging process. In hepatic cytoplasm of 3 and 6 months group, a loss of mitochondria and disruption of RER were observed, as well as accumulation of lipid droplets within the lipid-storing cell and increased dense body around the bile canaliculi. Moreover, the lipid droplets within the lipid-storing cell. a degree of accumulation was increased in 20 months. Especially, the fiber appeared in hepatic cytoplasm on 20 months group.

인터루킨-1α를 기관지 투입 후 나타난 폐세척액에서의 대식세포의 미세구조적 변화

박원학,이영만,조현국 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.2

인터루킨-1을 흰쥐의 기관지 내로 투여하였을 때 급성 부종성 폐손상(acute edematous lung injury)이 유발되며, 폐세척액 내 호중구가 증가되고 surfactant의 양도 증가한다. 대식세포는 증가된 surfactant에 기인한 탐식작용 활동이 증가하여 세포 내 환상의 구조물들이 증가하나 정상군과 비교하여 형태적으로 세포기관의 구조에서 차이를 보이고 있다. 이러한 세포구성은 세포간 기능상의 차이를 나타내는 것이며, surfactant의 순환과 연관성이 있을 것으로 사료된다. 본 연구에서 정상군에 있어서 폐포강 내 대식세포가 환상의 구조물들을 합성하는 형태를 보였으며, 이것은 surfactant의 재합성과 분비작용과 매우 밀접한 연관성이 있음을 보여 주는 것이다. In order to investigate the recycling of the pulmonary surfactant in association with morphological changes in macrophage after interleukin-1 α(IL-1) induced lung injury, an acute lung injury was induced by instillation of IL-1 into the trachea, Numbers of neutrophils and phospholipid content were increased significantly(P<0.01) in IL-1 treated BAL(brochoalveolar lavage) compared to control rat. By increased phagocytosis, the lamellar structures in the macrophges of IL-1 treated rats' BAL were increased and the compositions of the cellular organelles were changed in comparison to control rat. This difference in compositions of cellular organelles denotes difference of functions in macrophages between control and IL-1 treated rats. As macrophages have been said to implicate (in the difference in the recycling of pulmonary surfactant, it is highly probable that the difference in compositions of cellular organelles is closely related to the recycling of pulmonary surfactant. In the present study circular structures were synthesized in the cytoplasm of the macrophages in BAL of normal rats. Based on these experimental results, it is suggested that macrohages might synthesize surfactant during recycling of surfactant in the lung.

흰쥐에 Xylene 반복 투여가 Xylene의 대사에 미치는 영향

전태원,이혜자,윤종국,이상일,조현국 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1999 Journal of biomedical laboratory sciences Vol.5 No.1

실험동물에 xylene의 반복 투여가 이물질의 대사에 어떠한 영향을 미치는지를 알아보기 위하여 흰쥐에 m-xylene과 olive oil의 동량혼합액을 체중 100g당 0.25ml씩 2일 간격으로 1, 4, 8, 12 및 16회 복강으로 투여한 다음 마지막 투여 24시간 후에 처치하여 다음과 같은 결과를 얻었다. 요 중 m-methylhippuric acid 함량은 m-xylene 4회 투여군의 경우 1회 투여군에 비하여 약 56%의 유의한 증가를 보였으며 이후 12회 투여까지 유사한 결과를 나타내었으나 m-xylene 16회 투여시에는 xylene 1회 투여군 치와 유사한 치로 감소되었다. 그리고 간조직의 microsomal aniline hydroxylase와 alcohol dehydrogenase 활성은 m-xylene 12회 투여시 까지는 대체적으로 점진적인 증가를 보였으나 이후 16회 투여시에는 12회 투여군에 비하여 유의한 감소를 보였다. 또한 aldehyde dehydrogenase 활성은 m-xylene 투여 회수에 비례해서 전 실험기간 동안 감소되었으며 특히 16회 투여군에서 본 효소활성의 현저한 감소를 보였다. 한편 본 실험조건에서 투여기간에 따른 진자현미경적 미세구조의 변화는 초기에 활면소포체의 증식이 보이다가 16회 투여군에서는 활면소포체가 감소되고 조면소포체가 증가되었다. 이상 실험결과는 흰쥐에 있어서 xylene 투여 회수에 따라서 요 중 m-methylhippuric acid의 농도 변동이 초래되며 이는 효소단백 유도에 따른 xylene 대사효소 활성 변동에 기인된 결과로 생각된다. To evaluate the effect of repeated treatment of xylene on its metabolism, m-xylene (0.25ml of 50% in olive oil/100 g body weight) has been intraperitoneally given to the rats 1, 4, 8, 12 and 16 times every other day. m-Xylene was once more administered to the animals after 24 hrs since last injection of it. And then the animals were sacrificed after 24 hrs. Four times xylene treated rats showed the significantly elevated urinary m-methylhippuric acid, compared to those treated with the singe dose of m-xylene with the continued similiar high levels of urinary m-methylhippuric acid up to the animals pretreated 12 times and then those treated 16 times defined the significantly decreased urinary m-methylhippuric acid compared to those treated 12 times. On the other hand, hepatic aniline hydroxylase and alcohol dehydrogenase activities demonstrated a gradual increase from the first group to the 12 times xylene-treated animals, but those treated 16 times showed the significantly decreased value compared with the 12 times treated-group. And aldehyde dehydrogenase activities in rats treated with m-xylene 8, 12 or 16 times were significantly decreased compared to those pretreated one or four times. In the early stage of xylene administration, proliferation of SERs were seen whereas SERs were decreased and RERs were clearly increased in xylene-treated rats 16 times. These results indicate that the frequency of xylene injection may influence upon the changes in xylene metabolite, m-methylhippuric acid and it may be due to induction of xylene metabolizing enzymes.

Simultaneous Detection of Three Tobamoviruses in Cucurbits by Rapid Immunofilter Paper Assay

Gug-Seoun Choi,Jae-Hyun Kim,Bong-Nam Chung,Hyun-Ran Kim,Yong Mun Choi 한국식물병리학회 2001 Plant Pathology Journal Vol.17 No.2

Myrrha Induces Enhanced Keratinocyte Growth Factor in Scald Burn Skin of Rat

Cho, Hyun-Gug,Lee, Hang-Woo,Kim, Jeong-Ki,Lee, Yong-Deok,Chung, Hyeung-Jae 대한의생명과학회 2001 Journal of biomedical laboratory sciences Vol.7 No.3

The present study was conducted to determine whether skin spread of Myrrha has an effect on the cell regeneration as well as wound healing following dermal scald burn injury, keratinocyte growth factor (KGF) level was analyzed immunologically in conjunction with the histological changes occurred in skin tissue. The KGF contents in Myrrha skin spread group, which shows cell regeneration ability in skin tissue after burn, increased after 5 hours. After 24 hours, 'the content of Myrrha skin spread group is noticeably higher than at 5 hours postburn. After 72 hours, KGF was decreased compared to at 24 hours postburn. Acceleration effect of KGF production in Myrrha skin spread group was high. Together with the result of histological changes, skin spread of Myrrha reduced protein degeneration and edema in dermis, and induced proliferation of epithelial cells. The data suggest that Myrrha has accelerate cell regeneration and wound healing in case of scald burn skin by spreading of paste.

치의사 수급의 적정성에 대한 연구 -치의사수, 치의직무지수를 중심으로

조현국 ( Hyun Gug Cho ),박덕영 ( Deok Young Park ),마득상 ( Deuk Sang Ma ),정세환 ( Se Hwan Jung ) 대한구강보건학회 2004 大韓口腔保健學會誌 Vol.28 No.3

브레이크 디스크의 잔류응력이 제동 시 떨림 현상에 미치는 영향

장국현(Gug Hyun Jang),장호(Ho Jang),김희열(Hee Yeol Kim),김정규(Jeong Kyu Kim) 한국트라이볼로지학회 2009 한국트라이볼로지학회 학술대회 Vol.2009 No.11

Residual stress of the grey iron brake disk was studied by neutron scattering. A vacuum heat treatment schedule was employed to compare the change of residual stress in three principal orientations (i.e., normal direction, radial direction, and hoop direction) before and after stress relaxation. Dimensional variation of the disks after the heat treatment was also measured using a static disk thickness measurement device. The result shows that the residual stress in the disk was decreased after the heat treatment and the relative amounts of stress relaxation were dissimilar in three principal orientations. Permanent disk distortion was observed after dynamometer tests that simulated hot judder during brake applications, confirming the effect of residual stress on hot judder phenomena. The root cause of the residual stress induced thermal distortion was also discussed by examining the high temperature tensile strength of grey iron up to 600℃.