콩 펩타이드의 항암 활성

이형주 서울대학교 농업개발연구소 2000 농업생명과학연구 Vol.4 No.-

An anticancer peptide from soy protein was purified and isolated. Defatted soy protein was hydrolyzed with thermoase and its hydrophobic peptides were extracted with ethanol. The peptide extract was fractionated by XAD-2 adsorption, gel filtration chromatography, and different C18 HPLCs. Anticancer activity of each fraction was assayed by measuring in vitro cytotoxicity on P388D1, a mouse monocyte macrophage cell line. IC50 value of a peptide fraction from Sephadex G-25 chromatography was 0.16 mg/ml. This peptide fraction at 1 mg/ml significantly affected cell cycle progression by arresting P388D1 at G2/M phases. Finally purified peptide from analytical C18 HPLC was nonapeptide of which molecular weight was 1157 Da and the sequence was X-Met-Leu-Pro-Ser-Tyr-Ser-Pro-Tyr.

몸적 주체의 회복 : 에밀리 디킨슨의 '원융'개념을 중심으로 on Emily Dickinson's Circumference

김형태,권현주 대구대학교 인문과학 예술문화연구소 2003 人文科學硏究 Vol.25 No.-

As a woman poet, Emily Dickinson digged out her identity in a patriarchal society. With double identity as an indweller and outsider in that society, she became qualified to grasp the whole on an individual and social level. She noticed the passiveness and destructive force of the marginal resulted from excessive emphasis on symbolic order. Simple and popular chant form was used omitted and distorted with complex and abstract theme. The tension and irony between content and form was analogous with that of being. The oppression and dependence of symbolic values through a transcendental signifier was revealed. Emily Dickinson's resistance against the Master's order was complex and strategic. Her efforts can be focused on the pursuit of circumference overcoming a dichotomy of spirit and flesh. She analogized herself with 'the only Kangaroo among the Beauty'. A kangaroo was regarded as a typical poetic example as it keeps growing in its lifetime and grassed twilight, a mixture of light & darkness. As a spiral expansion process, circumference was a continuous process to grasp individual and social totality. This process was on the road to make the dejected and the flesh recover the wholeness in society. In other words, this dynamic process was the way to revive a bodily subject through disruption and reconstruction. As one circle's periphery can be an inner core or a starting point of another larger circles, circumference meant the marginal and the direction in motion at the same time.

Lactococcus lactis ssp. lactis ATCC7962의 갈락토tm 및 유당 대사 유전자

이형주 서울대학교 농업개발연구소 1999 농업생명과학연구 Vol.3 No.-

The genes involved in the metabolism of galactose and lactose in Lactococcus lactis ssp. lactis ATCC7962(L. lactis 7962) through Leloir pathway were cloned and they constituted the operon. The gene order reflected the sequential reactions of metabolism of galactose: galA(galactose permease) - galM(aldose mutarotase) - galK(galactokinase) - galT(galactose 1-phosphate uridylyltransferase) - lacA(galactoside acetyltransferase) - lacZ(β-galactosidase) - galE(UDP-glucose 4-epimerase). The lac genes were intervened between galT and galE. Northern blot revealed that galE was constitutively transcribed by its own promoter and induced by several upstream promoters with galactose and lactose. The inducible upstream promoters indicated catabolite carbon repression (CR).

북미자유무역협정의 정치경제학: 그 성격과 성과를 중심으로

임현진,이성형,송주명 서울대학교 국제지역원 1996 국제지역연구 Vol.5 No.2

이 글은 1994년도에 출범한 북미자유무역협정의 역사적 배경에서 출발하여 협정에 이르기까지의 각국의 협상 목표와 전략, 그리고 세계경제 전반에 미치는 영향과 협정의 주요 내용, 마지막으로 지난 3년간의 공과를 간단히 검토하고 있다. 세계적 차원에서 경제적 경쟁이 심화되면서 북미 3국은 기존의 상호의존성을 토대로 자유무역지대를 창출하는데 성공하지만 각국의 목표는 조금씩 다르다. 미국은 상대적으로 경제적 이익보다는 멕시코의 안정이라는 정치적인 목료를 고려하였고, 멕시코는 발전모델의 변경에 소요되는 재워조달이라는 경제적 이유가 가장 고려되었으며, 캐나다는 반면에 불참의 불이익을 회피하기 위해 북미자유무역협정에 가입하였다. 3년간 실험 끝에 역내의 무역창출 효과는 상당히 제고되었고 산업내 무역도 활발하게 전개되고 있지만 무역분쟁은 아짇고 크게 줄지 않고 있다. 초기의 낙관론과 달리 북미자유무역협정이 미주자유무역지대로 발전할 가능성은 상당히 약하다. This study examines the historical background of the NAFTA of 1994, negotiation targets and strategies of each country involved, its impact on the world economy as a whole, and its successes and failures over the past three years. While the three North American countries succeeded in establishing a free trade area based on reciprocity in the context of deepening economic competition in the world economy, the objectives of each country in this process slightly differ each other: The United States places more emphasis on a political objective-the stabilization of Mexico; Mexico is more interested in obtaining financial resources for remolding the existing economic development model; and Canada tried to avoid the possible economic disadvantages due to the boycott. After the three year economic experiment, the effect of trade creation within the trade area and intra-industry trade have been enhanced, whereas the trade disputes among the members still remain high. But the possibility of developing the NAFTA into the AFTA seems to be modest at the moment.

복싱에어로빅 참여자의 건강증진생활양식에 따른 생활만족도에 관한 연구

최윤동,김정주,김형돈 경희대학교 체육대학 한국체육과학연구소 2003 體育學論文集 Vol.31 No.-

Among various life-time sports, the boxing-aerobic as aerobic exercise which unites the boxing with the aerobic has been recently attracted public attention due to usefulness and effectiveness. Naturally, the boxing-aerobic enables all over the body to equally train and each body part light and flexible as well as participants to be on a effective diet. The purpose of this study is to look into minutely life satisfaction according to health promoting life styles of participants in boxing-aerobic among life-time sports as well as offers fundamental information for self-control and life quality improvement of boxing-aerobic participants. Specially, it aims to identify (1) differences in health promoting life styles in accordance with demographic characteristics of participants in boxing-aerobic (2) differences in life satisfaction in accordance with demographic characteristics of participants in boxing-aerobic (3) inter-relation between health promoting life styles and life satisfaction of participants in boxing-aerobic. In order to accomplish the purpose, a convenient sample of 130 boxing-aerobic participants was selected, and the questionnaire for life styles test used for this study was made out by Seo, Yeon-Ok(1994) and for life satisfaction test used was made out by Park Hong-Seok(2001). The background variables of the study were demographic characteristics such as age, education, average income, marriage, career to participate, blood type and religion. Through the analysis of established hypothesis and collected data, it is concluded that the higher monthly average income, the higher health promoting life styles and the higher life satisfaction.

Kluyveromyces lactis에 의한 유청으로부터 과일향 성분의 생성

김소미,이형주 한국산업미생물학회 1991 한국미생물·생명공학회지 Vol.19 No.5

유당발효 효모인 Kluyveromyces lactis ATCC 8585를 이용하여 유청에서 과일향 풍미성분의 생산성을 향상시키기 위하여 3차에 걸쳐 NTG를 처리하고 geraniol의 항균력에 내성을 지니는 변이주 중 모균주에 비해 yeasty-flavor는 약하나 fruity-flavor가 강한 변이주를 선발하고 K. lactis 450K라 명명하였다. 3일간 배양시킨 발효액을 pentane-dichloromethane(2:1) 용매로 추출하여 얻은 oleoresin을 gas chromatography로 분석한 결과, 모균주보다 변이주가 더 많은 종류와 양의 향기성분을 생산한 것으로 나타났다. 변이주의 배양액으로부터 잠정적으로 확인된 주요향기성분은 테르펜류로는 myrcenol 등이, 알콜류로는 cis-3-hexenol, n-hexanol, 에스테르류로는 ethyl isovalerate, cis-3-hexenyl n-butyrate, n-amyl-n-hexanoate, phenyl ethyl n-propioate, 케톤류로는 methyl vinyl ketone, 기타 성분으로 vanillin, 3-methylcoumarin 등이었다. To enhance the productivity of fruit flavor compounds from whey by the lactose fermenting yeast, Kluyveromyces lactis ATCC 8585 was treated with N-methyl-N'-nitro-N-nitro-soguanidine(NTG). After the NTG treatments, a mutant showing resistance to antifungal activity of geraniol, and strong fruity but low yeasty flavor was selected and named as K. lactis 450 K. Flavor compounds from 3-day culture broth were extracted with pentane-dichloromethane(2:1) and the concentrated oleoresins were analyzed by gas chromatography. The mutant strain produced more classes and larger amount of flavor compounds than the parent strain. Tentatively identified volatile compounds from the culture of the mutant were: terpenes such as myrcenol; alcohols such as cis-3-hexenol, n-hexanol; esters such as ethyl isovalerate, cis-3-hexenyl n-butyrate, n-amyl-n-hexanoate, phenyl ethyl n-propioate; ketones such as methyl vinyl ketones; other compounds such as vanillin, 3-methylcoumarin.

라덱스 혼입률에 따른 철근콘크리트의 휨파괴 거동특성

정원경,김동호,이주형,임홍범,윤경구 江原大學校 産業技術硏究所 2002 産業技術硏究 Vol.22 No.A

Reinforced concrete(R/C) is commonly used to structures because they have many merits that compressive strength, economy and so on. However, reinforced concrete has a crack at the tensile section which is due to the relatively lower tensile strength than its compressive strength. Latex modified concrete(LMC) has higher tensile and flexural strength than the ordinary portland cement, due to the interconnections of hydrated cement and aggregates by a film of latex particles. The purpose of this study was to investigate the flexural behavior of reinforced concrete beam with latex modified concrete, having the main experimental variables such as concrete types(ordinary portland cement concrete, latex modified concrete), latex contents(0%, 15%), flexural steel ratios(0.012, 0.0235), and with/without shear reinforcement. The beam of LMC showed considerably higher initial cracking loads and ductility than that of OPC, but, similar to ultimate strength and deflection. This might be attributed to the interlocking of hydrated cement and aggregates by a film of latex particles, water retention due to hydrophobic, and colloidal properties of the latexes resulting in reduced water evaporation. The beam with latex modified concrete could be adopted at field for controlling and reducing the tensile crack due to its higher tensile strength.

라텍스 개질 콘크리트의 강도발현 및 내구특성

윤경구,홍창우,이주형,이종명 강원대학교 석재복합신소재제품연구센터 2001 석재연 논문집 Vol.6 No.-

본 연구는 라텍스 혼입률이 5%, 10%, 15%, 20%로 변화할 때 라텍스 개질 콘크리트의 내구 특성을 평가하는데 있다. 라텍스를 콘크리트에 혼입한 라텍스 개질 콘크리트는 보통 콘크리트의 색상과 성형성, 작업성를 갖으며, 약 20%~35%의 단위수향이 감소된다. 그리고 양생후, 콘크리트는 시멘트수화물과 라텍스 입자의 필름막에 의해 연결된 골재로 구성된다. 일반적으로 라텍스 양이 증가하면 콘크리트의 휨강도는 증가하는데, 이것은 라텍스가 시멘트 수화물과 골재사이의 점착력을 증가시키기 때문으로 판단된다. 라텍스 개질 콘크리트와 보통콘크리트와의 상대투수성을 측정하기 위하여 염소이온투과시험법을 사용하였다. 그 결과 라텍스 개질 콘크리트의 투수성은 보통콘크리트의 투수성보다 매우 낮음을 알 수 있었다. 이것은 라텍스가 미세공극을 충진하고 라텍스 입자의 필름막에 의해 골재와 시멘트수화물이 연결 때문이다. 또한 라텍스가 공극주위로 필름막을 형성하기 때문에 공기량이 투수성에 영향을 미치지 못한다는 것을 알 수 있었다. 보통콘크리트에서는 동결융해저항성을 증가시키기 위해 공기연행제를 필요로 하지만, 라텍스 개질 콘크리트에서는 적절한 양생이 이루어지면 추가적인 공기연행제는 요구되지 않는다. 또한 라텍스 혼입률이 증가함에 따라 추가적인 동결융해저항성의 증가는 나타나지 않았다. This study focused on the investigation of strength development and durability of latex modified concrete(LMC) as the latex content variated such as 5%, 10%, 15%, and 20%. When latex was added to concrete, the workability of LMC was quietly increased, compared to that of conventional concrete. This resulted in the decrease of the required water as 20% to 35% in order to obtain a same slump. In general, increasing the amount of latex decreased compressive strength a little but increased flexural strength quite a lot. This might be due to interconnection by a film of latex particles between hydrated cement and aggregates. This, also, might attribute to bonding between the hydrated cement and aggregates. The rapid chloride permeability test was used to evaluate the relative permeability of latex-modified concretes and conventional concretes. The results showed that the permeability of latex-modified concretes was considerably lower than that of conventional concretes tested, which might be due to the latex filled in voids and interconnections of hydrated cement and aggregates by a film of latex particles. Because of latex formed film surrounding the air void, air contents and voids does not effect to the permeability of latex modified concrete.

조기탈형을 위한 라텍스 개질 초속경 콘크리트의 개발 및 강도발현 특성

이봉학,홍창우,이주형,정원경 강원대학교 석재복합신소재제품연구센터 2001 석재연 논문집 Vol.6 No.-

현재 스틸렌-부타디엔 라텍스(styrene-butadiene latex)를 혼입한 콘크리트는 광범위하게 사용되고 있다. 교면포장용으로 라텍스 개질 콘크리트(LMC)의 사용은 일반 콘크리트와 다르지 않지만 라텍스에 의해 콘크리트의 특성을 향상시킨다. 그러나, 이와 같은 라텍스 개질 콘크리트와 다르지 않지만 라텍스에 의해 콘크리트의 특성을 향상시킨다. 그러나, 이와 같은 라텍스 개질 콘크리트는 장시간 양생을 요하는 문제로 인해 긴급을 요하는 곳에는 적용할 수 없다. 따라서 본 연구에서는 초속경시멘트를 사용한 라텍스 개질 콘크리트를 개발하고, 이에 따른 작업성 및 강도발현특성을 분석하는데 그 목적이 있다. 실험결과, 지연제와 소포제를 사용한 라텍스 개질 초속경 콘크리트(RSLMC)의 강도발현을 확인할 수 있었으며, 제안된 최적배합은 초속경성의 강도발현으로 인해 현장에서 긴급보수에 적용이 가능함을 알 수 있었다. 따라서 라텍스 개질 초속경 콘크리트는 교면포장, 포장체, 빌딩등의 보수에 적용이 가능할 것으로 판단된다. Concrete containing styrene-butadiene latex is widely used, nowadays, as a protective system for bridge. The usage of latex modified concrete (LMC) as an overlay system is not all that different from any quality concrete, but the improved properties impart to the concrete by the latex. However, this may not be used for repair works because the bridge lane could not be closed for several weeks for curing. Therefore, this study focused on the development of rapid-setting cement latex modified concrete (RSLMC), and on the investigation of workability and strength development. As a result, this study clearly validated the strength development of RSLMC by introducing a retarder and an antifoam agent. The proposed optimized concrete mixtures might be satisfactorily applied for the repair works in fields, because of its rapid strength development, respectively. Therefore, RSLMC could be used in many repair or overlay works such as bridge deck, pavement or building.

통조림의 初溫 및 冷却이 殺菌에 미치는 影響

姜君中,崔相道,李乙熙,閔永鳳,金泰圭,金炯甲,朱玉守 진주산업대학교 1989 論文集 Vol.27 No.-

살균전 통조림 내용물의 온도와 냉각처리 유무가 통조림의 살균에 미치는 영향을 조사하였다. 살균시간을 최소화하고 레토르트안과 통조림냉점의 두 곳에서 온도를 감지하여 치사율의 적산치를 계속적으로 이행할 수 있는 컴퓨터시스템을 이용하였다. 8% 벤토나이트 분산액을 301-5호관에 채우고 반복 실험을 통해서 내용물의 초온의 차이와 냉각수의 처리 유무 공히 살균시간에 뚜렷한 영향을 미쳤다. The effect of initial can temperatures and water cooling treatment on the cans for thermal sterilization was described. The accumulated lethal rate method based on microcomputer-based electronic system has introduced in this experiments to minimize the process time and provide precise temperature measurements at 2 points with continuous calculation of the lethal rate. According to a series of experiments which were performed by using 301-5 cans of 8% bentonite suspension, there was significant effect in accumulated lethal rate initiated in different temperatures and treated water cooling treatment.