거짓말탐지기(POLYGRAPH) 검사 사례

김정길,최효택 內務部 國立科學搜査硏究所 1996 國立 科學 搜査 硏究所 年報 Vol.28 No.-

DNA 증거의 집단유전학적 분석을 위한 SAS시스템

이효정,이혜승,한길로,이재원,황적준 大韓法醫學會 2000 대한법의학회지 Vol.24 No.1

DNA analysis has become one of the most powerful tools in forensic inference for human identification and is now used worldwide. It is used to be statistical technique for the individual identification of a civil and criminal action. The purpose of this article is computerization of the statistical technique for the population study and DNA evidence analysis. The system using SAS/AF and SAS/SCL is the graphic user interface and the correspondence of the changed experimental circumstances

확대 영상의 해상도 향상에 관한 연구

강길봉,김장형,김정효 제주대학교 공과대학 첨단기술연구소 2004 尖端技術硏究所論文集 Vol.15 No.1

Generally, the still image magnification uses image growing, interpolation and restoration in order to get magnificated image. Still image Magnification does not get high-resolution image because, amount of information is not sufficient. In this thesis, we proposed the hybrid method of high resolution image magnification. that is, to get high resolution image, the image processed by both lowpass filtering and highboost filtering. Result of apply proposed method to Lena image, we gained result of enhancement more better than formerly simple technique.

항AK3 단클론 항체의 single chain fragment variable(ScFv) 제작 및 생화학적 특성 연구

김백길,김효준 한양대학교 이학기술연구소 2002 이학기술연구지 Vol.5 No.-

단클론 항체는 항원에 대한 높은 특이성을 갖는다. 이러한 특성은 혈액이나 조직에 존재하는 특정 항원을 검출하기 위해 널리 이용되고 있다. 그러나 혈액 내에 존재하는 human anti mouse antibody (HAMA)와 같은 plasma protein 에 의한 단클론 항체와의 결합에 의해 특이성이 심하게 저하되는 현상이 나타난다. 최근 Fc 부분이 제거된 단클론 항체가 혈액내 비특이적 결합을 줄일 수있음이 ㅂ조고되고 있으며, 이러한 목적으로 phage display system을 이용한 항체공학이 Fc부분을 제거하기 위해 방법론적으로 활용되고 있다. 본 연구에서는 phage display system을 이용하여, 항 adenylate kinase isoenzyme 3(AK3) 단클론 항체 SJA3-86으로부터 single chain fragment variable gene(ScFv)을 cloning하였고, E.coli expression system을 통해 재조합 항체 ScF86을 발현시켰다. ScF86의 cDNA 염기배열로부터 유추한 CDR1, 2 및 3는 negative charge의 amino acid가 다수 포함되어 염기성 효소인 AK3를 전기적으로 효율적인 인식이 이루어짐을 확인하였고, parental antibody인 SJA3-86이 conformational epitope보다 denatured epitope을 효과적으로 인식하는 항원-항제 반응서으을 유지하고 있었다. 그러나 Fc 부분이 절단되고, 아미노산10개로 구성된 linker peptide가 도입된 결과 항원에 대한 Kd값은 1.1×10-9으로 원래의 약60%의 친화력을 단백공학에 의한 형태변화에도 불구하고 항원의 인식능력은 크게 변하지 않았다. 이러한 결과로 ScF86은 혈액내 비특이적 결합을 제거한 항체공학적 단클론 항체로서 임상적으로 유용하게 활용될 수 있을 것으로 사료된다. Abstract : As monoclonal antibody has high specificity for a antigen, it is widely used for the detection of spexific antigen in blood or tissues. however, non-specific binding of monoclonal antibody in blood is still troublesome. Recently, it is reported that antibody without Fc reactivity can decrease non-specific binding in blood. To remove Fc reactivity antibody engineering technique using phage display system has been used developed. By utilizing phage display system we constructed ScFv(Single chain Fragment variable)fragment of monoclonal antibody against human adenylate kinase isozymes 3(AK3) expressed as a single peptide in E.coli. From the analysis of amino acids seuuence we verified that the CDRs of ScF86 contained many negatively charged amino acids, which were reasonable to interact with AK3 by charge interaction. However the deletion of Fc region and addition of linker peptide caused the lower 1.I× 10-9 M of ScF86 which is the affinity decrease by that of 60% of parentalantibody. This deviation is very small one compaired with other general protein engineering on receptor-ligand reactivities and the lowered affinity is not socntical in the further usage of the antibody. The refgre, we successfully constructed there combinant antibody ScF86 against AK3 that eliminate the Plasma-derivated non-specific bindings in immunochemical studies.

양식 은어의 미포자충 감염예에 대한 병리조직학적 관찰

조병열,강형길,강효주,류갑민,이재영,박남규,허민도 한국어병학회 2003 한국어병학회지 Vol.16 No.1

1998년 9월 말경, 경남 지역의 양식 은어(Plecoglossus altivelis)에서 미포자충증이 발생하였다. 채집 당시, 하루 약 200마리씩의 폐사가 10일째 진행되고 있었으며 약 10%의 누적폐사율을 나타내었다. 병어는 입올림을 하거나 기면 상태에 있었으며, 대부분 체색흑화 및 복부팽만 소견을 보이고 있었다. 아가미, 아가미 뚜껑, 각종 내장, 복강벽 및 생식소에 직경 1~3㎜의 흰색 결절이 다수 형성되어 있었다. 감염 어종, 기생충의 형태 및 xenoma 분포 장기의 다양성에 근거하여 Glugea plecoglossi에 의한 감염증으로 추정되었다. 병리조직학적 검사를 실시한 결과, 광범위한 장기에 걸쳐 xenoma를 형성하고 있었으나 xenoma 인접조직에 변성 변화는 없었다. 아가미 새변, 비장, 두신 및 체신의 조혈영역에 심한 울혈 소견이 인정되었고, 간장, 심근 및 신세뇨관 국소에는 응고 내지 액화괴사 소견이 확인되었다. 따라서, xenoma의 인접조직에 대한 물리화학적인 영향보다는 xenoma형성 장기 및 조직에 야기된 국소 순환장애가 은어의 폐사와 직접적인 관련을 갖는 것으로 사료되었다. On September in 1998, a microsporidian infection was recognized in ayu, Plecoglossus altivelis, farmed on Kyongnam province of South Korea. Cumulative mortality was around 10% in 10 days. Infected fish which were piping for air near the surface of the water or in the asphyxic, lethargic condition revealed darkening of body and abdominal distention. Numerous whitish nodules up to 3㎜ in size were observed throughout most of body organs and tissues including gill, operculum, peritoneal wall and organs. Xenomas were also histologically confirmed in multiple internal organs with the evidences suggesting circulatory disturbance. Based on the morphology of spore and xenoma, and the distribution of xenomas in organs and tissues, this disease was diagnosed to be a microsporidiosis caused by Glugea plecoglossi. The mortality might be deeply related to the local circulatory disturbance by xemonas rather than the mechano-chemical effect of xenomas on adjacent tissues.

미세혈관 협심증 환자의 임상상 및 지표에 관한 연구

박상호,온영근,김현정,이태훈,길효욱,김현건,박수진,송해정,한대희,방덕원,신원용,이내희,김성구,권영주 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Background : Microvascular angina or syndrom X is heterogenous and encompasses different pathogenetic entities. Several studies suggest that microvascular angina have good prognosis and occurs predominantly in postmenopausal women. Lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and fibrinogen are the independent risk factor of acute coronary syndrome and are the chief underlying cause of atherosclerosis. We investigated clinical characteristics and markers in patients with microvascular angina. Methods : We studies 23 patients diagnosed with microvascular angina and 16 control group with negative treadmill exercise test and no significant coronary angiogram. We investigated clinical characteristics in patients group and evaluated symptom during a follow-up period of 4.2± 2.5 month. Exercise time in treadmill exercise test was compared between patients and control group. We assessed the level of markers, such as lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and ESR in two groups. Results : There is no difference in age between two groups. Patients group had a tendency to increase in female, hypertension, DM, and smoking history, but there was no statistical significance difference. Exercise duration time was significantly decreased in patients with microvascular angina (p<0,05). Serum lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholestrol, Triglyceride, ESR, Fibrinogen and Homocystein value were no significant difference between microvascular angina and control group. Only CRP was significantly high in patients with microvascular angina (p<0.05). Conclusion : There was no statistical significance difference in age, sex, hypertension, DM and smoking history between two groups but patients group had a tendency to increase the prevalence in hypertension, DM and smoking history, female. Also, Symptom improvement and disappearance in follow-up was presented in 11 patients of 19(57.9%). Exercise tolerance was worse in patients group than control group. CRP value was significantly higher in patients group than control group. There was no statistical significance difference in lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, ESR and Fibrinogen between two groups.

Analysis of Photosynthetic and Fluorescence Reaction on the Leaves of Streawberry under Shading Treatments During Cultivation of Winter

Hyo Gil Choi,Joon Kook Kwon,Jae Han Lee,Kyoung Sub Park 한국원예학회 2015 한국원예학회 학술발표요지 Vol.2015 No.10

Relationship between Pulmonary Surfactant Protein and Lipid Peroxidation in Lung Injury due to Paraquat Intoxication in Rats

( Hyo Wook Gil ),( Mi Hae Oh ),( Kee Min Woo ),( Eun Young Lee ),( Myung Ho Oh ),( Sae Yong Hong ) 대한내과학회 2007 The Korean Journal of Internal Medicine Vol.22 No.2

Background: Pulmonary damage resulting from lipid peroxidation is a principal effect of paraquat intoxication. The host-defense functions of surfactant are known to be mediated by the surfactant proteins A and D (SP-A and SP-D, respectively). The primary objective of this study was to evaluate the variations over time in levels of surfactant protein and lipid peroxidation (LPO) in lung tissue following free-radical-induced injury. Methods: 42 adult, male, Sprague-Dawley rats were administered intraperitoneal injections of paraquat (35 mg/kg body weight). SP-A and SP-D levels were determined via Western blot. LPO in the left lung homogenate was measured via analyses of the levels of thiobarbituric acid-reactive substances. Results: LPO levels peaked at 6 hours, with no associated histological changes. SP-D levels increased until hour 12 and declined until hour 48; SP-D levels subsequently began to increase again, peaking at hour 72. SP-A levels peaked at hour 6, declining thereafter. Conclusions: We suggest that in the early phase of paraquat injury, SP-D levels reflect alveolar damage and that de novo synthesis of SP-D takes 72 hours. Levels of SP-A, on the other hand, reflect abnormalities in the surfactant system in the late stage of paraquat intoxication. Surfactant proteins may play a role in protecting the lungs from reactive oxygen injury. A time-dependent variation has been observed in the levels of surfactant proteins A and D following paraquat injury, and it has been suggested that these proteins play a role in the protection of lung tissue against ROS-induced injuries.

Changes in Characteristics of Chlorophyll a Fluorescence on Tomato Fruits during Ripening under Various Environmental Conditions

Hyo Gil Choi,Jae Yeon Jeong,Jae Myun Lee,Si-Yong Kang,Seong Heo,Dong Sub Kim,Seok Kyu Jung,Yong-Soon Park 한국원예학회 2023 한국원예학회 학술발표요지 Vol.2023 No.10

Fruit Characteristics of Five Strawberries: Domestic Breeding Cultivars

Hyo Gil Choi,Jook Kook Kwon,Kyoung Sub Park,Sun Yi Lee 한국원예학회 2014 한국원예학회 학술발표요지 Vol.2014 No.5