RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단

배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.

자기주도학습력향상을 위한 수업모형 연구

현정숙,박영태 동아대학교 교육대학원 1999 동아교육논총 Vol.25 No.-

It is very important for the learners to develop their ability to judge, select subjectively and learn information and knowledge necessary for themselves in the current open society towards learning. In this respect, for the development of this ability, school education must put a stress upon the premises that learners can prepare for the basis of learning for continuing education in the course of life and that they can develop the self-directed learning ability in order to think themselves and to search for a truth. Therefore, this study aims to develop instructional model so that the learners can improve a self-directed learning ability through school education. The questions for research are raised for a successful investigation as follows: First, what characteristics do the constituent factors of children have in their self-directed learning ability? Second, what is the concrete plan for the improvement of constituent factors in their selfdirected learning ability? Third, what is the integrated intructional model for the improvement of self-directed learning ability in children? The conclusions can be obtained in this study as follows: First, it can be proved that children have an ability in all the constituent factors of self-directed learning ability such as openness, self-concept, intrinsic motivation, autonomy, creativity, ability to problem-solving, self-evaluation and so on, but that their ability in specific constituent factors show lower than adults. Second, the individual teaching plans for the improvement of self-directed learning ability can include the establishment of purpose, preparation of conditions for learning, forning human relationship, supporting learning, the organization of learning group, proper homework, learning contract, the chances of the various experience and practice, self-evaluation, the role of teachers and so on. Third, the plans for the improvement of self-directed learning ability through analysis of study and instructional model which the improvement of children’s self-directed learning ability are meaningful learning matters, teacher’s activities, learning context, environment of learners, meaning creation of learners, learning contract. Fourth, the integrated instructional model for the improvement of self-directed learning ability consists of learners’ activities, and teachers’ activities are suggested in order to guide, promote and instruct learners’ activities. This is to put an emphasis upon the mutual cooperation between children and their teachers after due consideration into characteristic of children’s development during instruction. The activities of learners include the choice of subject for learning, meaning creation of learners, the establishment of concreate learning target self-evaluation, evaluation of comrade and self-reinforcement. The activities of teachers include preparation of conditions for learning based upon the various conditions of information, the choice of subject for learning and its suggestion, offering motive for learning creation, controlling members of group, evaluation of performance, and teachers’ reinforcement. Teaching by this integrated instructional model will enable children to develop their own ability of the specific constituent factors wanting, and this will promote elementary school children’s self-directed learning ability.

SD Rats를 이용 1-hexene의 흡입독성 연구

김현영,임철홍,정용현,이권섭,이성배,이준연,한정희,전윤석,이용묵 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.4

The purpose of this study was to investigate the acute (4 hours) and repeated-dose (6 hours a day, 5 days a week, 4 weeks) toxic effects of I -hexene on Sprague- Dawley (SD) rats which were treated by inhalation. The results were as follows; I. The median lethal concentration (LC_(50)) was estimated 52,694 ppm (confidence limit 95%; 49,494~55,447 ppm) in acute inhalation. Abnormal clinical signs related to the l-Hexene were not observed with the acute inhalation dose. Gross findings of necropsy revealed on evidence of specific toxicity related to the 1-hexene, II. By repeated inhalation exposure the body weight of male were more or less reduced by the dose of 2,500 ppm and 5,000 ppm compared with control group. However there were no significant variation hematology and blood biochemistry for the exposed rats compared with the control rats. Abnormal clinical signs and gross findings of necropsy related to the 1-hexene were not shown. In conclusion when we exposed I-hexene to SD rats for 4 weeks, 5 days per week, 6 hours per day, the Lowest observed effect level (LOEL) was over 2,500 ppm and Non observed effect level (NOEL) was below 500 ppm.

S.D. Rats를 이용 1, 1-Dichloro-1-fluoroethane의 아민성 흡입독성 연구

김현영,이성배,임철홍,김철우,점용현,한정희,전윤석,최수영,강대봉,이용묵 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.1

There were no specific effects for test materials on Sprague-Dawley (S.D.) rats in clinical symptoms, amounts of food intakes, weight changes, laboratory findings, and pathology after whole body l, l-Dichloro-1 -fluoroethane (used as coolant, metal cleaner and solvents) exposure(0, 1,500, 3,000, and 6,000 ppm) for 13 weeks (6 hour/day, 5 days/week). However, the loss of capillary vessels in eyeball (pupil) was observed in a female rat among 6,000 ppm group. Though there was a tendency for MCHC (Mean Corpuscular Hemoglobin Concentration) in rat to be decreased (p<0.05), it was not regarded as abnormal because the values were within normal limits. In asthma-stimulation related evaluations, there was also a tendency for inflammatory cell counts in bronchoalveolar lavages to be increased. But it had no statistical significance, and also no dependency on sex and the exposed concentration. Based on this result, the non observed effect level (NOEL) induced by 1, 1-Dichloro-l -fluoroethene inhalation was evaluated in groups with 3,000 ppm below (S .D. Rats, 13 weeks). Finally, it was concluded that the short term exposal of 1, 1-Dichloro-l-fluoroethane is not considered as a asthma stimulant by inhalation despite of some study limitations such as test animals use and short-term exposure .

도시유역의 침수방지를 위한 유수지 능력개선에 관한 연구

이영화,조현경 慶山大學校 1998 論文集 Vol.16 No.1

This study aims at the capability improvement of detention basin for inundation prevention of urban area by RRL model. The chosen detention basin to analyze in this study is located at Pohang industrial district. The RRL model is computed a probable flood to evaluate the capability of detention basin for each return period. The results obtained by RRL model as follows: (1) The capabilities of detention basin and pumps are estimated. (2) To improve the capability of the detention basin, the dredged depth of the detention basin and the number of pump are calculated for return period.

격막형 액막에 의한 코발트 이온 및 니켈이온의 분리

金春泳,鄭奉佑,李鉉哲 全北大學校 1993 論文集 Vol.35 No.-

The separation of the cobalt ion and nickel ion was carried out which was based on the principle of coupling facilitated transport. The principle of coupling facilitated transport was developed and validated by predicting the cobalt ion and nickel ion fluxes through membrane as a function of hydrogen ion concentration in the feed solution, carrier concentration in the membrane, and content of metals ion in the feed solution, flow rate of the feed solution and acid concentration in the stripping solution. Diffusion coefficients of Co-di(2-ethyl hexyl) phosphoric acid and Ni-di(2-ethyl hexyl) phosphoric acid through polytetrafluroethylene membrane were 1.2×10^7㏖/sec and 5.5×10^8㏖/sec, respectively.

어머니의 성격특성이 양육스트레스와 양육행동에 미치는 영향

전현진, 김영희 충북대학교 생활과학연구소 2013 생활과학연구논총 Vol.17 No.2

The purpose of this study was to examine effects of mother's personality on parenting stress and behaviors. Subjects of this study were 425 mothers of young children aged 3-5 years old attending 2 kindergartens and 4 child care centers located Cheong-ju city and Chungbuk province. Data were analyzed by frequency, percentage, Cronbach's α, and Pearson's product-moment correlation coefficient using the SPSS 12.0 program, and path analysis was done using AMOS 7.0 program. The results of this study were as follows: First, mother's open-mindedness of personality had a direct effect on parenting stress caused by dysfunctional parent-child interaction on while mother's extraversion of personality had directly impacted on parenting stress aroused by her own parenting. However, mother's neuroticism of personality had directly affected on all areas of parenting stress which it indicated mother's neuroticism was the most strong impactor on parenting stress. Second, parenting stress caused by child and mother had direct effects on mother's controlling parenting while all areas of parenting stress caused by child, mother, and dysfunctional parent-child interaction had direct effects on mother's rejective parenting behavior. Mother's affectionate parenting behavior was directly affected by parenting stress caused parent-child dysfunctional interaction. Third, mother's open-mindedness, extroversion, neuroticism of personality had indirect effects on mother's parenting behavior mediated by parenting stress.

크롬(Ⅵ)의 체내 흡수와 소실속도에 관한 연구

김현영,이성배,임철홍,이권섭,정용현,이종성,한정희,전윤석,황호순,이용묵 한국산업위생학회 2003 한국산업보건학회지 Vol.13 No.1

The CrO3 mostly used in plating. metal surface disposal, leather, cosmetic manufacturing, as an experiment material by repeatedly inhaling and exposure the male S.D. rats at a 0.00, 0.2., 0.50, 1.25 mg/㎡ concentration(particle size: 0.5-0.5 aerosol)6hours a day, 5day a week in 13weeks comparing with 2weeks, 8weeks of recovery group about the noxiousness of the experiment animal and the reduce scale of the CrO3 in the internal organ especially in blook and respiratory organ with the period of convalescent and clearance. The experiment results which we received are as follows. 1. In blood the RBC, HGB and HCT experiment, rats with 0.20, 0.50 mg/㎡ concentration showed that there were some decreases but not dependent. The kidneys absolute weight compared with control group was reduced intentionally(p〈0.05) and the lungs absolute weight compared with control group showed intentional increase(p〉0.05). 2. After the exposure of the experiment material, the whole blook, l\blood plasma and red blood cell in blood by (x): the period of convalescent, per (y); the decreasing of Cr concentration, was y=66.51 e -0.057x, y=67.2 e-0.101x, y=70.01 e-0.030 in 0.50 mg/㎡ exposure concentration by calculating the clearance coefficient of correlation, and the half life (day)was estimated 12.0, 6086, 23.0 each. 3, After the exposure of the experiment material, the experiment animals lung, liver and kidneys by(x); the period of convalescent, per (y); the decreasing of Cr conentration, was y=1808 e-0.00493x, y=12.02e-0.0297x, y=67.61 e-0.0292x in 0.50mg/㎡ exposure concentration by calculating the clearance coefficient of correlation, and the half life(day)was estimated 140.6, 23.3, 23.7, each, and including lung, liver with all of the experiment internal organs, the Cr clearance decreased as the exposure concentration increased.

유전자 알고리즘을 이용한 유출모형의 매개변수 추정

조현경,이영화 한국환경과학회 2003 한국환경과학회지 Vol.12 No.10

The genetic algorithm is investigated for parameters estimation of SED (storage - effective drainage) model from the Wi-stream watershed in Nakdong river basin. In the practical application of model. as a number of watershed parameters do not measure directly, it is desirable to make a good estimation from the known rainfall and runoff data. For the estimation of parameters of the SED model using the genetic algorithm, parameters of Green-Ampt equation(SM, K_(s)) for the estimation of an effective rainfall and initial storage(y_(in)) used in SED model are obtained a regression equation with 5, 10, 20 days antecedent precipitation. And as a consequence of computation, the parameters were obtained to satisfy the proposed objective function. From the comparison of observed and computed hydrographs, it shows a good agreement in the shape and the rising limb, peak, falling limb of hydrograph, so the SED model using the genetic algorithm shows a suitable model for runoff analysis in river basin.

Wilson's Disease 2예

고지영,안병철,이현옥,권오상,최진영 중앙대부설한국의과학연구소 1989 한국의과학 Vol.21 No.3