Inhibition of oxidative stress induced‑cytotoxicity by coptisine in V79‑4 Chinese hamster lung fibroblasts through the induction of Nrf‑2 mediated HO‑1 expression

Hyeon‑Gyun Jo,Cheol Park,이혜숙,Gi‑Young Kim,Young‑Sam Keum,Jin Won Hyun,Taeg Kyu Kwon,Yung Hyun Choi,Su Hyun Hong 한국유전학회 2021 Genes & Genomics Vol.43 No.1

Background Coptisine is a natural alkaloid compound and is known to have multiple benefcial efects including antioxidant activity. However, whether it can protect lung fbroblasts from oxidative damage has not been studied yet. Objectives To investigate the potential inhibitory efect of coptisine against oxidative stress in V79-4 lung fbroblast cells. Methods V79-4 cells were treated with H2O2 (1 mM) in the presence or absence of coptisine (50 µg/ml), N-acetyl cysteine (NAC, 10 mM) or zinc protoporphyrin IX (ZnPP, 10 µM) for the indicated times. The alleviating efects of coptisine on cytotoxicity, cell cycle arrest, apoptosis, reactive oxygen species (ROS) production, DNA damage, mitochondrial dynamics, and inhibition of ATP production against H2O2 were investigated. Western blot analysis was used to analyze the expression levels of specifc proteins. Results Coptisine inhibited H2O2-induced cytotoxicity and DNA damage by blocking abnormal ROS generation. H2O2 treatment caused cell cycle arrest at the G2/M phase accompanied by increased expression of cyclin-dependent kinase (Cdk) inhibitor p21WAF1/CIP1 and decreased expression of cyclin B1 and cyclin A. However, these efects were attenuated in the presence of coptisine or NAC. Coptisine also prevented apoptosis by decreasing the rate of Bax/Bcl-2 expression in H2O2-stimulated cells and suppressing the loss of mitochondrial membrane potential and the cytosolic release of cytochrome c. In addition, the activation of nuclear factor-erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was markedly promoted by coptisine in the presence of H2O2. However, zinc protoporphyrin IX, a potent inhibitor of HO-1, attenuated the ROS scavenging and anti-apoptotic efects of coptisine. Conclusions Based on current data, we suggest that coptisine can be used as a potential treatment for oxidative stress-related lung disease.

진주지역 제조기업의 산업재해 실태분석과 대책방안

최진현,강호욱 경상대학교 경남문화연구소 1983 慶南文化硏究 Vol.6 No.1

The process of manufacturing enterprise takes the potential injury agents (moving equipment, machines and tools, hazards, etc). The industrial accidents due to these occasion happen unexpectedly. Safety management, in the manufacturing enterprise, are a moral action for worker, and improve the production efficiency and decrease the accident cost. In this study, industrial accidents are analyzed on the actual condition for 339 manufacturing enterprise, and select the 9 sampling enterprise. We simulate the relationship between accident cost and safety management cost. So, We suggest the safety management counterplan. The actual condition of the industrial accidents were analyzed by I.L.O. statistical analysis equations, which are expressed by (1) and (2). FR=N/H×10^6 …(1) SR=S/H×10³ …(2) Logical development of the alternatives for safety management counterplan based on the structure of industrial accident. We derived characteritics of the industrial accidents, and suggested a few counterplan of industrial accidents for manafacturing enterprises in Jin-Ju area.

휴대용 핵종 분석기의 유용성 평가

김지현(Ji Hyeon Kim),전성진(Seong Jin Jin),제재용(Jae Yong Je) 한국방사선학회 2015 한국방사선학회 학술대회 논문집 Vol.2015 No.춘계

본 연구는 휴대용 핵종분석기를 이용하여 부산지역의 지역별 환경방사선을 측정하여 휴개용 핵종분석기의 유용성을 평가하고자 한다. 측정 대상은 부산의 중심에 위치한 부산진구 소재 송상현 광장을 중심으로 9곳의 환경 방사선을 측정하였다. 측정결과 부산역과 부산대학교를 제외한 대부분의 지역에서 환경방사선은 비슷한 결과를 나탄내었다. 검출된 핵종은241Am, 133Ba, 54Mn등이 검출되었고 핵종분석기에 내장된 핵종정보가 부족하여 알 수 없는 핵종들도 검출되었다. 실험에 사용한 휴대용 핵종분석기는 분해능이 8% 정도로 자연방사성 핵종과 인공방사성 핵종을 핵종별로 정확하게 분석하기 위해서는 제조사의 개선이 필요한 것으로 판단된다. This research aims at measuring the environmental radioactivity at each area of Busan using a potable radio-isotope identification system, and assessing the utility of the potable radio-iosotope identification system. As for the target for measurement, the environmental radioactivities of nine areas centered around Songsanghyeon Square in Jin-gu located in the center of Busan were measured. As the measurement result, the environmental radioactivities were similar in most of the areas except in Busan station and Busan University. The nuclides, such as 241Am, 133Ba, 54Mn and so on, were detected, and some nuclides whose identity was unknown because of lack of information built in the radiation spectrum analyzer were also found. The potable radio-isotope identification system used in the experiment had about 8% of resolving power, so for more precise analysis into the nuclides of natural background radiation and artificial radiation in each type, the manufacturer needs to improve the analyzer.

WIPI 기반 모바일 3D 게임엔진의 설계와 구현

형대진,김승구,조현준,박경환 동아대학교 정보기술연구소 2006 情報技術硏究所論文誌 Vol.14 No.1

In this paper, we introduce the method for developing the mobile 3D game engine based on the WIPI platform which has been proposed as the mobile standard platform. Our game engine is designed so that it supports OpenGL-ES and JSR-184 that are mobile 3D graphic APIs. Our game engine has a mobile emulator operated on PC and SDK to support mobile programming in PC environments. Mobile 3D game developers can use the APIs at various levels. Also, the game engine is developed in modular in order to support various game genres.

올레산의 비율에 따른 아데노신의 경피 투과의 영향

남현빈,정세진,구민석,오예진,김용민,이훈희,윤수정,임정균 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2

In addition to the development of functional cosmetics, technology to increase percutaneous absorption rate when applied to the skin is an important task. In this experiment, oleic acid was used as a skin absorption promoter, and its effect on transdermal permeability of adenosine was studied and analyzed using a skin-like membrane. As a result of the experiment, it was investigated that the permeability coefficient and concentration of adenosine are proportional to the content of oleic acid. This indicates that a high proportion of oleic acid is effective as a skin absorption promoter.

진행성 폐 종양 환자에서 종양 내 OK-432 주입술의 효과

정수현,김성빈,박진희,탁희상,옥철호,장태원,정만홍,천봉권 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Background: Intratumoral injection of OK-432 produced significant antitumor effects. There are reports that OK-432 was injected directly into tumor tissue on gastrointestinal tracts and HCC tissue, producing decrease in serum alpha-fetoprotein level. We adopted this technique for treating locally advanced lung cancer. The object of this study is to evaluate the local therapeutic efficacy, side effects of injection for treating symptomatic mass. Methods: Twenty lung cancer patients (13 males and 7 females) underwent sonography guided OK-432 injection. We analyzed relationships between response of OK-432 and response of skin test, histology and age. Pain that patients feel was evaluated by VAS (Visual Analog scale). Results: A significant decrease of tumor size was observed. The mean pre-injection diameter of the lesions was 54.7±11.3mm, PR rate were 15.0% (3/20) and minimal change (50%<tumor size decreased.) patients were 35.0% (7/20). The VAS score before injecting OK-432 into the lesion was 5±3, this was decreased to 2.5±1.5 (p=0.04). Object response was significantly correlated with skin response, adenocarcinoma, and age. Conclusion: A significant decrease of tumor size and pain was observed. So OK-432 is a valuable treatment option for patients with symptomatic lesion from advanced lung cancer who are not amenable to further therapy.

한국증권시장의 효율성에 대한 연구 : 필터기법을 이용

김용현,정진호 한세대학교 1999 敎授論叢 Vol.15 No.-

파 雄性不稔性의 遺傳分析 : Allium fistulosum L.

宋基現,金鎭漢 忠北大學校 農業科學硏究所 2002 農業科學硏究 Vol.19 No.-

Mlae streilith is one of the major tools for F1 Hybrid seed production in vegetable. Until 1980s, the F1 hybrid seed production in welsh onion (Allium fistulosum L) was accomplished by using genic male sterile lines However, the 50% male fertile plants had to be elminated from the seed production field by hand. In 1989, a new male sterile mutant (MS216) was found on the Breeding and Research Station of Hungnong, which is assumed for cyto;lasmic male sterilty. This experiment was performed to study on the inheritance of male sterility in MS216 The inheritance of male sterility in MS216 was analyzed by determining segregation ratio obtainde from crossings with MS216 and male fertile lines or GMS line. F1,BC1 and BC2 were taken from back-crossing with MS216for recipient and 5 different amle fertile lines for ercurrent In the same way F1, BC1 and BC2 by crossing with MS216 for recipient and 2 different GMS lines for recurrent was obtainde All plants of the F1, BC1 and BC2 population derived from the crossing with MS216 and 5 different male fertile lines showde male sterility in the field, suggesting that the MS216 has a factor influencing male sterility in its cytoplasm And also, from the experiment of crossing between the MS216 and the two GMS lines it was found that genic factor in the GMS lines was not related to the male sterility of MS216 These results show that MS216 has typical cytoplasmic male sterility not affected by any karyogene

두부방사선규격사진 촬영 시 유도방법에 따른 자연두부자세의 차이 및 재현성에 관한 연구

송진명,이기헌,,황현식 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.5

두부방사선규격사진에서는 매 촬영 시마다 두부의 수직적 회전 정도를 일정하게 유지하기 위하여 두부자세의 재현이 필요하다. 본 연구는 유도방법에 따른 자연두부자세의 수직적 두부회전 정도의 차이를 비교하고 각 두부자세의 재현성을 평가하기 위하여 시행되엇다. 20대 성인 남녀 30명을 연구대상으로 하여 head posture allgner를 이용한 방법(HPA법)과 self balance posture를 이용한 방법(SBP법)으로 자연두부자세를 유도하여 측모 및 정모두부방사선규격사진을 각각 한 달 간격으로 촬영한 후, 측모두부방사선규격사진 상에서 유도 방법에 따른 수직적 두부회전 정도의 차이를 비교하는 한편, 측모 및 정모두부방사선규격사진에서 시간 간격을 두고 동일 방법으로 유도하여 촬영한 두 사진 상의 동일 계측항목 간 차이를 비교함으로써 각 두부자세의 유도방법에 대한 재현성을 평가하였다. 연구결과 SBP법으로 유도한 자연두부자세는 HPA법에 의해 유도된 자연두부자세보다 평균 2.79˚ 고개를 숙인 자세로 나타났다. 또한 HPA법 또는 SBP법을 이용하여 시간 간격을 두고 각각 2회 촬영한 측모두부방사선규격사진과 정모두부방사선규격사진 상의 계측치는 모든 계측항목에서 통계적으로 유의한 차이를 보이지 않는 것으로 나타났다. 본 연구 결과는 HPA법과 SBP법으로 유도한 자연두부자세가 모두 재현성이 있음을 나타내는 것으로, 두부방사선규격사진 촬영 시 재현성을 높이기 위하여 HPA법과 더불어 SBP법도 임상적으로 사용될 수 있음을 시사하였다. The purpose of this study was to compare the degree of vertical head rotation and to evaluate the reproducibility of natural head posture (NHP) according to two guiding methods. The head posture aligner (HPA) method and the self balance posture (SBP) method. The subjects consisted of 30 adults. On the first day. Lateral and frontal cephalometric radiographs were obtained through the two guiding methods. One month later. lateral and frontal cephalometric radiographs were obtained again through both guiding methods. The degrees of vertical head rotation of both guiding methods were compared and the reproducibility was evaluated for each guiding method. A comparison of the degrees of vertical head rotation for the two methods revealed that the vertical head posture was lower in the SBP method than in the HPA method by an average of 2.79˚. All measurements obtained using the HPA and SBP methods with a time interval of one month did not show any significant difference in lateral and frontal cephalometric radiographs. The results of the present study suggest that the SBP method may be used as an alternative to the HPA method in case the HPA method can not be applied.

수용자의 광고태도가 광고회피에 미치는 영향

소현진,박범순 한국방송광고공사 2008 광고연구 Vol.0 No.78

광고회피 행위에 영향을 주는 요소들에 대한 연구는 꾸준히 이루어져 왔으며, 그 중 수용자의 광고에 대한 태도(attitude toward advertising in general)가 광고회피의 주요한 선행요인 중 하나로 제시되고 있다. 그러나 광고태도는 다양한 하위요소를 포함하는 다차원적 개념이라는 기존 연구에도 불구하고 아직 다차원적 개념으로서의 광고태도를 광고회피 연구에 도입하는 시도는 매우 드물다. 따라서 본 연구는 (1)광고태도를 구성하는 각 하위요소들과 광고회피와의 관계를 분석하고, (2)광고태도의 하위요소들 중 어떤 요소가 광고회피를 예측하는 데 가장 유용한 변인인지를 알아보고자 한다. 또한 (3)각 하위요소들과 광고회피 간의 관계가 수용자의 인구통계학적 특성에 따라 달라지는지를 탐색적으로 연구해보고자 하였다. 연구 결과를 요약하자면 첫째, 광고의 사회적인 영향에 대한 평가 및 경제적인 영향에 대한 평가는 광고회피와 약한 부(-)적 상관관계를 보였다. 둘째, 광고의 개인적인 효용성 중 광고의 정보적인 유용성은 광고회피에 유의미한 영향을 미쳤다. 그러나 광고의 쾌락적인 유용성에 대한 평가는 광고회피에 통계적으로 의미 있는 영향을 미치지 않았다. 또한 광고의 거시적 효과와 개인적 효용성에 대한 평가 차이에 대해서는 광고의 거시적 효과에 대한 평가보다는 광고의 개인적 효용성에 대한 평가가 광고회피를 예측하는 데 더 유용한 변인으로 나타났고, 개인적 효용성에 대한 평가 중에서도 정보적인 유용성에 대한 신념이 광고회피에 대한 가장 효과적인 예측변인이라는 결과를 보였다. 이러한 광고태도와 광고회피 간의 관계는 응답자의 인구통계적인 속성에 관계없이 일관되게 나타났다. Attitude toward advertising in general(AG) has been considered one of important antecedents of advertising avoidance. Although it has been well-documented that AG is a multi-dimensional construct containing several sub-factors, it is rare to investigate the relationship between individual attitude factors and ad avoidance. The objectives of this study are (1) to examine the relationship between attitude factors and advertising avoidance; (2) to investigate which attitude factor is the strongest predictor of ad avoidance; and (3) to explore whether the relationship of attitude factors and ad avoidance varies depending on demographic variables. The data were collected from a mail survey of general American adult consumers. Results show that all attitude factors, including consumer perception on advertising’s economic effect, societal effect, informational usefulness, and hedonic usefulness, are significantly and negatively correlated with ad avoidance. However, ANOVA analyses found only consumer perception on advertising’s economic effect and informational usefulness have significant influence on ad avoidance. The pattern of relationship between individual attitude factors and ad avoidance was consistent across different age and gender groups. Hierarchical regression analysis discovered consumer perception on personal use of advertising is the more powerful predictor of ad avoidance than the perception on economic/societal effect of advertising. Among informational usefulness and hedonic usefulness which together measured consumer perception on personal use of advertising, informational usefulness was found to be the strongest predictor of ad avoidance. Theoretical and practical implications of this study were finally discussed.