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A Young Yoon,Sujin Yun,HyeJin Yang,Yoon Hwa Lim,Haekwon Kim 한국발생생물학회 2014 발생과 생식 Vol.18 No.4
Previously we have shown that human abdominal adipose derived-stem cells (ADSCs) could aggregate during the high-density culture in the presence of human serum (HS). In the present study, we observed that human cord blood serum (CBS) and follicular fluid (HFF) also induced aggregation. Similarly, porcine serum could induce aggregation whereas bovine and sheep sera induced little aggregation. qRT-PCR analyses demonstrated that, compared to FBS-cultured ADSCs, HScultured cells exhibited higher level of mRNA expression of CLDN3, -6, -7, -15, and -16 genes among the tight junction proteins. ADSCs examined at the time of aggregation by culture with HS, BSA, HFF, CBS, or porcine serum showed significantly higher level of mRNA expression of JAM2 among JAM family members. In contrast, cells cultured in FBS, bovine serum or sheep serum, showed lower level of JAM2 expression. Immunocytochemical analyses demonstrated that the aggregates of HS-cultured cells (HS-Agg) showed intense staining against the anti-JAM2 antibody whereas neither nonaggregated cells (HS-Ex) nor FBS-cultured cells exhibited weak staining. Western blot results showed that HS-Agg expressed JAM2 protein more prominently than HS-Ex and FBS-cultured cells, both of latter reveled weaker intensity. These results suggest that the aggregation property of ADSCs during high-density culture would be dependent on the specific components of serum, and that JAM2 molecule could play a role in the animal sera-induced aggregation in vitro.
Yoon, A Young,Yun, Sujin,Yang, HyeJin,Lim, Yoon Hwa,Kim, Haekwon The Korean Society of Developmental Biology 2014 발생과 생식 Vol.18 No.4
Previously we have shown that human abdominal adipose derived-stem cells (ADSCs) could aggregate during the high-density culture in the presence of human serum (HS). In the present study, we observed that human cord blood serum (CBS) and follicular fluid (HFF) also induced aggregation. Similarly, porcine serum could induce aggregation whereas bovine and sheep sera induced little aggregation. qRT-PCR analyses demonstrated that, compared to FBS-cultured ADSCs, HS-cultured cells exhibited higher level of mRNA expression of CLDN3, -6, -7, -15, and -16 genes among the tight junction proteins. ADSCs examined at the time of aggregation by culture with HS, BSA, HFF, CBS, or porcine serum showed significantly higher level of mRNA expression of JAM2 among JAM family members. In contrast, cells cultured in FBS, bovine serum or sheep serum, showed lower level of JAM2 expression. Immunocytochemical analyses demonstrated that the aggregates of HS-cultured cells (HS-Agg) showed intense staining against the anti-JAM2 antibody whereas neither non-aggregated cells (HS-Ex) nor FBS-cultured cells exhibited weak staining. Western blot results showed that HS-Agg expressed JAM2 protein more prominently than HS-Ex and FBS-cultured cells, both of latter reveled weaker intensity. These results suggest that the aggregation property of ADSCs during high-density culture would be dependent on the specific components of serum, and that JAM2 molecule could play a role in the animal sera-induced aggregation in vitro.
Development of an Efficient Tapered Slip-Form System Applying BIM Technology
Yoon, Hyejin,Chin, Won Jong,Kim, Hee Seok,Kim, Young Jin Scientific Research Publishing, Inc 2013 Engineering Vol.5 No.9
<P> Slip-form system constitutes the latest technology for the erection of elevated concrete pylons. This paper investigates the design of slip-form system applying BIM technology for the efficient development of the slip-form system. The considered pylon has a height of 10 m and presents the rectangular hollow section generally adopted in cable-supported bridges. The slip-form was thus designed to accommodate the tapered cross-section and changing thickness considering the continuous placing of concrete. In addition, the safety of the system was examined with regard to the various loads applied on the slip form along the construction. The design results could be verified visually through BIM and the applicability of the designed slip-form was validated in advance through virtual assembly and construction. </P>
Conceptual Design of Hybrid Cable-Stayed Bridge with Central Span of 1000 m Using UHPC
Yoon, Hyejin,Chin, Won Jong,Kim, Hee Seok,Keum, Moon Seoung,Kim, Young Jin Scientific Research Publishing, Inc 2013 Engineering Vol.5 No.9
<P> Ultra-high performance concrete (UHPC) is featured by a compressive strength 5 times higher than that of ordinary concrete and by a high durability owing to the control of the chloride penetration speed by its dense structure. The high strength characteristics of UHPC offer numerous advantages like the reduction of the quantities of cables and foundations by the design of a lightweight superstructure in the case of the long-span bridge preserving its structural performance through axial forces and structures governed by compression. This study conducted the conceptual design of a hybrid cable-stayed bridge with central span of 1000 m and exploiting 200 MPa-class UHPC. The economic efficiency of the conceptual design results of the hybrid cable-stayed bridge with central span of 1000 m and of Sutong Bridge, the longest cable-stayed bridge in the world, was analyzed. </P>
Hyejin Yang,Yoon Hwa Lim,Sujin Yun,A Young Yoon,김해권 한국발생생물학회 2013 발생과 생식 Vol.17 No.4
Human serum (HS) has been reported to induce aggregation of human eyelid adipose-derived stem cells (HEACs) during high-density culture in vitro. The present study focused on the role of cell adhesion molecules and gelatinases during HS-induced aggregation of HEACs. HS-induced aggregation occurred between 9-15 days of culture. Cells aggregated by HS medium (HS-agg) showed stronger expression of α2, α2B, αX, and CEACAM1 genes compared to non-aggregated cells in HS medium (HS-ex) or in control FBS-cultured cells. HS-agg were distinctly labeled with antibodies against α2, α2B, and αX proteins. Western blot results demonstrated that the two integrin proteins were greatly expressed in HS-agg compared to HS-ex and control FBS-cultured cells. Treatment of HEACs with anti-integrin α2 antibody during culture in HS medium delayed aggregation formation. HS-agg exhibited strong expression of MMP1 and MMP9 compared to HS-ex or FBS-cultured cells. Conditioned media from HS-culture showed remarkable increase of MMP9 gelatinolytic activity in comparison to those from FBS-culture. However, there was no change of TIMP mRNA expression in relation to the HS-induced aggregation. Based on these results, it is suggested that integrin α2, α2B, and αX, and MMP9 might play an important role in the HS-induced aggregation of HEACs.