http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Choi, Sun-Hye,Shin, Tae-Joon,Lee, Byung-Hwan,Chu, Dae-hyun,Choe, Han,Pyo, Mi-Kyung,Hwang, Sung-Hee,Kim, Bo-Ra,Lee, Sang-Mok,Lee, Jun-Ho,Kim, Dong-Hyun,Kim, Hyoung-Chun,Rhim, Hye-whon,Nah, Seung-Yeol Elsevier 2010 european journal of pharmacology Vol.637 No.1
<P><B>Abstract</B></P><P>The slowly activating delayed rectifier K<SUP>+</SUP> channels (<I>I</I><SUB><I>Ks</I></SUB>) are one of the main pharmacological targets for development of drugs against cardiovascular diseases. Cardiac <I>I</I><SUB><I>Ks</I></SUB> consists of KCNQ1 plus KCNE1 subunits. Ginsenoside, one of the active ingredient of <I>Panax ginseng</I>, enhances cardiac <I>I</I><SUB><I>Ks</I></SUB> currents. However, little is known about the molecular mechanisms of how ginsenoside interacts with channel proteins to enhance cardiac <I>I</I><SUB><I>Ks</I></SUB>. In the present study, we investigated ginsenoside Rg<SUB>3</SUB> (Rg<SUB>3</SUB>) effects on human <I>I</I><SUB><I>Ks</I></SUB> by co-expressing human KCNQ1 plus KCNE1 subunits in <I>Xenopus</I> oocytes. Rg<SUB>3</SUB> enhanced <I>I</I><SUB><I>Ks</I></SUB> currents in concentration- and voltage-dependent manners. The EC<SUB>50</SUB> was 15.2±8.7µM. However, in oocytes expressing KCNQ1 alone, Rg<SUB>3</SUB> inhibited the currents with concentration- and voltage-dependent manners. The IC<SUB>50</SUB> was 4.8±0.6µM. Since Rg<SUB>3</SUB> acts opposite ways in oocytes expressing KCNQ1 alone or KCNQ1 plus KCNE1 subunits, we examined Rg<SUB>3</SUB> effects after co-expression of different ratios of KCNE1 and KCNQ1. The increase of KCNE1/KCNQ1 ratio converted <I>I</I><SUB><I>Ks</I></SUB> inhibition to <I>I</I><SUB><I>Ks</I></SUB> activations. One to ten ratio of KCNE1 and KCNQ1 subunit is required for Rg<SUB>3</SUB> activation of <I>I</I><SUB><I>Ks</I></SUB>. Mutations of K318 and V319 into K318Y and V319Y of KCNQ1 channel abolished Rg<SUB>3</SUB> effects on KCNQ1 or KCNQ1 plus KCNE1 channel currents. The docked modeling revealed that K318 residue plays a key role in stabilization between Rg<SUB>3</SUB> and KCNQ1 plus KCNE1 or KCNQ1 subunit. These results indicate that Rg<SUB>3</SUB>-induced activation of <I>I</I><SUB><I>Ks</I></SUB> requires co-assembly of KCNQ1 and KCNE1 subunits and achieves this through interaction with residues K318 and V319 of KCNQ1 subunit.</P>
Lee, Byung-Hwan,Choi, Sun-Hye,Shin, Tae-Joon,Pyo, Mi-Kyung,Hwang, Sung-Hee,Kim, Bo-Ra,Lee, Sang-Mok,Lee, Jun-Ho,Kim, Hyoung-Chun,Park, Hye-Young,Rhim, Hye-Whon,Nah, Seung-Yeol Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.30 No.3
The flavonoid quercetin is a low molecular weight substance found in fruits and vegetables. Aside from its antioxidative effect, quercetin, like other flavonoids, has a wide range of neuropharmacological actions. The ${\alpha}$7 nicotinic acetylcholine receptor (${\alpha}$7 nAChR) has a $Ca^{2+}$-binding site, is highly permeable to the $Ca^{2+}$ ion, and plays important roles in $Ca^{2+}$-related normal brain functions. Dysfunctions of ${\alpha}$7 nAChR are associated with a variety of neurological disorders. In the present study, we investigated the effects of quercetin on the ACh-induced inward peak current ($I_{ACh}$) in Xenopus oocytes that heterologously express human ${\alpha}$7 nAChR. $I_{ACh}$ was measured with the two-electrode voltage clamp technique. In oocytes injected with ${\alpha}$7 nAChR cRNA, the effects of the co-application of quercetin on $I_{ACh}$ were concentration-dependent and reversible. The $ED_{50}$ was 36.1 + 6.1 ${\mu}m$. Quercetin-mediated enhancement of $I_{ACh}$ caused more potentiation when quercetin was preapplied. The degree of $I_{ACh}$ potentiation by quercetin preapplication was time-dependent and saturated after 1 min. Quercetin-mediated $I_{ACh}$ enhancement was not affected by ACh concentration and was voltage-independent. However, quercetin-mediated $I_{ACh}$ enhancement was dependent on extracellular $Ca^{2+}$ concentrations and was specific to the $Ca^{2+}$ ion, since the removal of extracellular $Ca^{2+}$ or the addition of $Ba^{2+}$ instead of $Ca^{2+}$ greatly diminished quercetin enhancement of $I_{ACh}$. The mutation of Glu195 to Gln195, in the $Ca^{2+}$-binding site, almost completely diminished quercetin-mediated $I_{ACh}$ enhancement. These results indicate that quercetin-mediated $I_{ACh}$ enhancement human ${\alpha}$7 nAChR heterologously expressed in Xenopus oocytes could be achieved through interactions with the $Ca^{2+}$-binding site of the receptor.
Sala cibi gen. nov., sp. nov., an extremely halophilic archaeon isolated from solar salt
Song Hye Seon,Kim Juseok,Kim Yeon Bee,Lee Se Hee,Whon Tae Woong,Roh Seong Woon 한국미생물학회 2022 The journal of microbiology Vol.60 No.9
Two novel halophilic archaeal strains, CBA1133T and CBA- 1134, were isolated from solar salt in South Korea. The 16S rRNA gene sequences of the isolates were identical to each other and were closely related to the genera Natronomonas (92.3–93.5%), Salinirubellus (92.2%), Halomarina (91.3– 92.0%), and Haloglomus (91.4%). The isolated strains were coccoid, Gram-stain-negative, aerobic, oxidase-positive, and catalase-negative. Growth occurred under temperatures of 25–50°C (optimum, 45°C), NaCl levels of 10–30% (optimum, 15%), pH levels of 6.0–8.5 (optimum, 7.0), and MgCl2 concentrations of 0–500 mM (optimum, 100 mM). Digital DNADNA hybridization values between the strains and related genera ranged from 18.3% to 22.7%. The major polar lipids of the strains were phosphatidyl glycerol, phosphatidyl glycerol phosphate methyl ester, and phosphatidyl glycerol sulfate. Genomic, phenotypic, physiological, and biochemical analyses of the isolates revealed that they represent a novel genus and species in the family Halobacteriaceae. The type strain is CBA1133T (= KACC 22148T = JCM 34265T), for which the name Sala cibi gen. nov., sp. nov. is proposed.
말초 및 중추신경계에서 칼슘채널 및 NMDA 매개 채널의 억제제로의 진세노사이드 Rg<sub>3</sub>의 효과
임혜원,Rhim, Hye-Whon 고려인삼학회 2003 Journal of Ginseng Research Vol.27 No.3
Alternative medicines such as herbal products are increasingly being used for preventive and therapeutic purposes. Ginseng is the best known and most popular herbal medicine used worldwide. In spite of some beneficial effects of ginseng on the nervous system, little scientific evidence shows at the cellular level. In the present study, I have examined the direct modulation of ginseng total saponins and individual ginsenosides on the activation of $Ca^{2+}$ channels and NMDA-gated channels in cultured rat dorsal root ganglion (DRG) and hippocampal neurons, respectively. In DRG neurons, application of ginseng total saponins suppressed high-voltage-activated $Ca^{2+}$ channel currents and ginsenoside Rg$_3$, among the 11 ginsenosides tested, produced the strongest inhibition on $Ca^{2+}$ channel currents. Occlusion experiments using selective $Ca^{2+}$ channel blockers revealed that ginsenoside Rg$_3$ could modulate L-, N-, and P/Q-type currents. In addition, ginsenoside Rg$_3$ also proved to be an active component of ginseng actions on NMDA receptors in cultured hippocampal neurons. Application of ginsenoside Rg$_3$ suppressed NMDA-induced [Ca$^{2+}$]$_{i}$ increase and -gated channels using fura-2-based digital imaging and patch-clamp techniques, respectively. These results suggest that the modulation of $Ca^{2+}$ channels and NMDA receptors by ginsenoside Rg$_3$ could be part of the pharmacological basis of ginseng actions in the peripheral and central nervous systems.ous systems.
쥐 - 뇌의 히스타민 - N - 메칠전달효소의 정제와 일반 특성 연구
임혜원,최명언 ( Hye Whon Rhim,Myung Un Choi ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.4
Histamine-N-Methyltransferase (HMT: E.C. 2.1.1.8) was purified by the methods of ammonium sulfate fractionation, DEAE-cellulose chromatography, hydroxylapatite chromatography, and gel filtration on Sephadex G-75. Overall purification was 280-fold with a recovery of 8%. The activity of HMT was determined by radioisotopic method with [^(14)CH₃]S-adenosylmethionine(SAM). The labelled SAM was prepared by rat liver SAM synthetase with [^(14)CH₃]-methionine. The specific activity of prepared HMT was 3.9 nmol/min/㎎ protein at pH 8.5. The K_m values of histamine and SAM were 12μM and 40μM, respectively. It was also examined the effects of some modification reagents on the enzyme activity. p-Chloromercuribenzoate and N-ethylmaleimide inhibited the enzyme activity, while iodoacetic acid, iodoacetamide and succinic anhydride activated the enzyme activity.
Purification and General Characterization of Rat Brain Histamine-N-Methyltransferase
임혜원,최명언,Rhim, Hye-Whon,Choi, Myung-Un 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.4
쥐 뇌의 histamine N-methyltransferase(HMT)는 $(NH_4)_2SO_4$ 분류법, DEAE-셀루로즈 크로마토그래피, hydroxylapatite 크로마토그래피와 Sephadex G-75에 대한 젤 여과법으로 정제되었다. 전체 정제는 8%의 회수율로 280배 정제되었다. HMT 활성도는 $[^{14}CH_3]$-S-adenosylmethionine(SAM)에 의한 방사성 동위원소법으로 결정하였다. 방사성 SAM은 $[^{14}CH_3]$ methionine을 가지고 쥐 간의 SAM synthetase에 의하여 합성하였다. 준비된 HMT의 specific actitity는 pH8.5에서 3.9nmol/min/mg protein이다. Histamine과 SAM에 대한 $K_m$값은 각각 $12{\mu}M$과 $40{\mu}M$이다. 효소활성에 대한 몇 가지의 변형시약을 검토했을 때 p-chloromercuribenzoate와 N-ethyl maleimide는 활성도를 감소시켰고 iodoacetic acid, iodoacetamide와 succinic anhydride는 효소의 활성도를 증가시킴이 관찰되었다. Histamine-N-Methyltransferase (HMT: E.C. 2.1.1.8) was purified by the methods of ammonium sulfate fractionation, DEAE-cellulose chromatography, hydroxylapatite chromatography, and gel filtration on Sephadex G-75. Overall purification was 280-fold with a recovery of 8%. The activity of HMT was determined by radioisotopic method with $[^{14}CH_3]$S-adenosylmethionine(SAM). The labelled SAM was prepared by rat liver SAM synthetase with $[^{14}CH_3]$-methionine. The specific activity of prepared HMT was 3.9 nmol/min/mg protein at pH 8.5. The $K_m$ values of histamine and SAM were $12{\mu}M$ and $40{\mu}M$, respectively. It was also examined the effects of some modification reagents on the enzyme activity. p-Chloromercuribenzoate and N-ethylmaleimide inhibited the enzyme activity, while iodoacetic acid, iodoacetamide and succinic anhydride activated the enzyme activity.
효소를 이용한 방사성 표지 s - Adenoxsyl - L - [ methyl - 14C ] methionine 의 생산
임혜원,박인원,최명언 ( Hye Whon Rhim,In Won Park,Myung Un Choi ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.5
S-Adenosylmethionine (SAM) serves as the major methyl group donor in biological system. This study describes an upgrade method for radiolabelled biosynthesis of SAM by methionine adenosyltransferase (MAT) in the presence of ATP and L-[^(14)C-methyl]methionine. The MAT was partially purified from rat liver and reoptimized the assay condition. The final yield of biosynthesis was better than 90% with relatively high specific radioactivity.