과열증기처리 반탄화 추출물의 항산화 효과 검증에 관한 연구

오근혜(Geun Hye Oh),남정빈(Jeong Bin Nam),양승민(Seung Min Yang),정원희(Won Hee Joung),정진산(Jin San Jeong),신종민(Jong Min Shin),강석구(Seog Goo Kang) 한국펄프·종이공학회 2018 펄프.종이기술 Vol.50 No.2

The antioxidant properties of the extracts from torrefied wood subjected to superheated steam treatment were investigated. Total polyphenolics, total flavonoids, DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity, reducing power, and FRAP (Ferric reducing antioxidant power) were determined to evaluate antioxidant activity. The total polyphenol content was 879.67±40.41 ㎎/mL at a concentration of 25 g/mL when the extract was subjected to a temperature of 300℃ for 10 min, and 759.67±25.17 ㎎/mL at a concentration of 25 g/mL at 350℃ for 10 min. The value of at 300℃ was 15.79% higher than that obtained at 350℃. Total flavonoid content was 111.18±3.55 ㎎/mL at a concentration of 25 g/mL at 300℃ for 10 min, and 80.58±2.58 ㎎/mL at a concentration of 25 g/mL at 350℃ for 10 min. The value at 300℃ was 37.97% higher than that obtained at 350℃. For the DPPH free radical scavenging activity, the highest scavenging activity was observed at a concentration of 6 g/mL. DPPH free radical scavenging activity at a concentration of 6 g/mL at 300℃ for 10 min was 89.83±0.03%, whereas at 350℃ for 10 min at the same concentration, it was found to be 87.99±0.1%. The value at 300℃ was 2.09% higher than that obtained at 350℃. Reducing power was determined to be 3.59±0.04 at a concentration of 25 g/mL at 300℃ for 10 min, and 2.92±0.1 at a concentration of 25 g/mL at 350℃ for 10 min. The value at 300℃ was 22.94% higher than that obtained at 350℃. FRAP was 1742±37 μM at a concentration of 25 g/mL at 300℃ for 10 min, and 1106±8 μM at a at 350℃ for 10 min. At 300℃, the FRAP value was 57.50% higher than that obtained at 350℃. Based on these results, we suggest that torrefied wood treated with superheated steam can be used in various applications because of its effective antioxidant properties.

가열 조건을 달리한 단호박 페이스트와 검 종류별 단호박 라떼의 품질특성

박보람,김나정,유선미,한귀정,김하윤,한혜민,신동선,신말식,Park, Bo-ram,Kim, Na-Jung,Yoo, Seon-Mi,Han, Gwi Jung,Kim, Ha Yoon,Han, Hye-min,Shin, Dong-Sun,Shin, Malshick 한국식품조리과학회 2015 한국식품조리과학회지 Vol.31 No.3

가열 조건에 따른 단호박 페이스트를 제조하기 위해, 단호박을 15분 간 초벌 증숙한 뒤, 고압가열 처리 0분(A), 10분(B), 20분(C), 40분(D) 실시하여 실험군의 품질특성 을 조사하였다. 그 결과 일반성분의 경우, 대체적으로 고 압가열 처리 유무에 따른 유의적 차이가 관찰되었으며, 고압가열 처리한 B, C, D 실험군의 수분함량, 조단백질, 조섬유가 고압가열 무처리군 A에 비해 감소하였고, 가용성 무질소물은 증가하는 것으로 나타났다. 가열 조건별 단호박 페이스트의 수용성식이섬유는 고압가열 20분 처리군인 C의 측정치가 2.02로 가장 높았으며 무처리군인 A의 1.60 보다 증가하였고, 고압가열 처리 40분의 경우 오히려 감소하는 것으로 나타났다. 색도의 L 값은 고압가열 무처리군인 A가 52.20에서 고압가열 처리 10분, 20분, 40분으로 시간이 증가함에 따라 각각 50.33, 49.46, 48.06으로 감소하였고, a 값과 b 값 또한 유의적인 차이를 보이며 감소하였다. 현미경을 통한 단호박 페이스트의 현탁액 입자를 관찰한 결과 카로티노이드를 포함하는 유세포가 관찰되었으며 고압가열 처리와 그 시간이 증가함에 따라 단호박 유세포의 변형이 뚜렷하게 관찰되었으나, 부유안정성 실험 결과 실험군 A, B, C, D 간 차이가 없었다. 이때, 가열조건의 선택은 수용성 식이섬유의 증가, 환원당 증가, 단맛의 관능특성이 유의적으로 높고, 전반적 기호도가 가장 우수한 것으로 나타난 고압가열 처리 20분인 C 실험군으로 결정하였다. 선택된 조건의 단호박 페이스트에 식품가공 시 널리 사용되는 검류인 xanthan gum, locust bean gum, guar gum을 단호박 페이스트에 종류별로 첨가하여 부유안정성을 확인하였는데, 이 결과 guar gum, locust bean gum, xanthan gum 순으로 부유안정성 효과를 나타냈으며 locust bean gum과 xanathan gum은 비슷한 정도의 효과를 보였다. 또한 관능검사를 통한 기호도 확인 결과 텍스쳐와, 전반적인 기호도가 가장 우수했으므로 locust bean gum(0.2%) 첨가 단호박 라떼의 품질이 가장 적절할 것으로 판단된다. For the production of pumpkin paste with respect to heating conditions, we steamed the pumpkin for roughly 15 min, heated it with high pressure treatment for 0 min (A), 10 min (B), 20 min (C), 40 min (D), and subsequently investigated the quality characteristics. Generally a significant difference was observed between the pumpkin paste treated with and without high-pressure heat. The values of water content, crude protein and crude fiber of the high-pressure heat-treated groups B, C, D were decreased compared with untreated group A. The soluble fiber in experimental group B sweet-pumpkin paste treated with high-pressure heat for 20 min was higher than the control, and the highest value at 2.02. Experimental group D sweet-pumpkin paste treated with high-pressure heat for 40 min was found to have a decreased soluble fiber content relative to the control. The L value for the color of the group A untreated control sweet-pumpkin paste (no high-pressure heating) decreased as the time increased from 10 min to 40 min, with L values of 50.33, 49.46, and 48.06, respectively. The b value for the color of the sweet-pumpkin paste also decreased, showing a significant difference. Taking into account all the results, we chose experimental group B in order to prepare sweet-pumpkin latte. We used 0.2% gum (xanthan gum, locust bean gum, guar gum) as a stabilizer. Sweet-pumpkin latte with xanthan and locust bean gum has a suspension stability effect that lasts 90 min. The L and b values of sweet-pumpkin latte with gums increase and a value decrease compared with the control. In terms of the overall acceptance of the sweet-pumpkin latte, the experimental group with xanthan gum scored the best.

반응표면분석에 의한 노랑느타리버섯 추출물의 추출조건 최적화

이혜진(Hye-Jin Lee),도정룡(Jeong-Ryong Do),정민유(Min-Yu Chung),김현구(Hyun-Ku Kim) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.10

노랑느타리버섯의 항산화 활성을 측정하여 효율적 추출조건을 예측하기 위해 마이크로웨이브 에너지, 에탄올 농도, 추출시간의 요인변수를 중심합성법에 따라 설정하여 반응표면분석을 통해 최적화 조건을 설정하였다. 노랑느타리버섯 추출물의 수율, 전자공여작용, SOD 유사 활성, 총 폴리페놀 함량에 대한 반응표면의 회귀식 R² 값이 각각 0.91, 0.87, 0.86 및 0.87로 분석되었고, 수율은 P<0.01, 항산화 활성이 P<0.05에서 유의적 수준을 나타냈다. 노랑느타리버섯은 요인변수에 따라 수율, 전자공여작용, 총 폴리페놀 함량의 경우 마이크로웨이브 에너지의 영향을 가장 많이 받았고, SOD유사 활성의 경우 에탄올 농도에 따른 유의적 차이를 보였다. 추출물의 각 특성을 모두 만족시키는 최적의 추출조건의 범위는 superimposing 하여 마이크로웨이브 에너지 71.48~92.84 watts, 에탄올 농도 55.01~71.66%, 추출시간 3~9분으로 예측되었다. Response surface methodology (RSM) was used to optimize extraction conditions of Pleurotus cornocopiae. Coefficients of determination (R²) for dependent variables ranged from 0.86 at 0.91. Maximum extraction yield was 40.81% under the following conditions: microwave power of 60.08 watts, ethanolic concentration of 12.33%, and extraction time of 6.86 min. Maximum extraction electron donating ability was 35.72% at 44.13 watts, 19.30%, and 4.21 min. Maximum extraction superoxide dismutase (SOD)-like activity was 34.87% at 114.01 watts, 65.88%, and 1.56 min. Maximum extraction total polyphenol content was 31.77 mg/g at 50.52 watts, 23.00% and 2.90 min. Based on the superimposition of four dimensional RSM with respect to extraction yield, electron donating ability, SOD-like activity, and total polyphenol content obtained under various extraction conditions, the optimum ranges of extraction conditions were as follows: microwave power of 71.48~92.84 watts, ethanol concentration of 55.01~71.66%, and extraction time of 3~9 min.

열처리에 의한 Morganella morganii와 Photobacterium phosphoreum 유래 Histidine Decarboxylase의 불활성화

박원민(Won-Min Pak),김꽃봉우리(Koth-Bong-Woo-Ri Kim),김민지(Min-Ji Kim),박지혜(Ji-Hye Park),배난영(Nan-Young Bae),박선희(Sun-Hee Park),안동현(Dong-Hyun Ahn) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.3

Histamine을 생성하는 Morganella morganii와 Photobacterium phosphoreum으로부터 crude histidine decarboxylase(HDC)를 추출하여 65~121°C로 열처리한 다음 균의 생육 및 효소 활성 변화에 대해 살펴보았다. 그 결과 M. morganii와 P. phosphoreum은 모든 열처리 조건에서 비 가열 처리구와 비교 시 균의 생육이 저해됨을 확인하였다. M. morganii와 P. phosphoreum 유래 HDC의 효소 활성은 65°C에서 90% 이상의 효소 활성이 저해됨을 확인하였고, 온도가 증가함에 따라 유의적으로 활성이 억제되는 것으로 나타났다. SDS-PAGE 결과에서는 65~100°C 범위에서 비가열 처리구와 비교 시 조효소액의 단백질 패턴의 변화가 크지 않았으나, 121°C에서 단백질 band의 강도가 크게 약해졌다. Native-PAGE에서는 65°C 처리 조건에서부터 단백질 패턴의 변화가 크게 나타났다. 따라서 가열처리(65~121°C)는 histamine 생성균인 M. morganii와 P. phosphoreum의 생육을 억제할 뿐만 아니라 HDC의 효소 활성도가 저해됨을 확인하여, 식품산업에서 적용되고 있는 열처리 조건에서 histamine 생성 억제에 큰 효과가 있는 것으로 생각한다. This study was performed to investigate the effects of various thermal treatments on the growth of Morganella morganii and Photobacterium phosphoreum and activity of crude histidine decarboxylase (HDC) obtained from M. morganii and P. phosphoreum. Crude HDC and the two strains were treated at 65°C/30 min, 80°C/10 min, 100°C/10 min, and 121°C/10 min. Activity of crude HDC decreased with increasing temperature. Viable cells counts of M. morganii and P. phosphoreum were not detected in any heated samples. SDS-PAGE patterns of heated HDC did not show significant differences up to 100°C. However, at 121°C, protein band intensity was weakened. In native-PAGE, there was a major change in the pattern of HDC at 65°C. These results suggest that thermal treatment can help to reduce histamine production by reducing HDC activity and growth of M. morganii and P. phosphoreum.

Determination of the optimum porosity for 2‑CEES adsorption by activated carbon fiber from various precursors

Hye‑Min Lee,Byeong‑Hoon Lee,Ju‑Hwan Kim,Kay‑Hyeok An,Soo‑Jin Park,Byung‑Joo Kim 한국탄소학회 2019 Carbon Letters Vol.29 No.6

In this study, we prepared ACFs with a high specific surface area from various precursors (rayon, pitch, and oxidized polyacrylonitrile-based fibers) by a steam-activation technique and investigated the effects of the micropore and mesopore fraction on 2-CEES adsorption behaviors. The activation time was precisely controlled so that the activation yield was in the range of 35–40% to ensure the mechanical properties of the ACFs. The N2 adsorption isotherm characteristics at 77K were confirmed by Brunauer–Emmett–Teller, Barrett–Joyner–Halenda and non-local density functional theory equations. The adsorption capacities of the ACF were measured by breakthrough experiments in the gas phase (750 μg/mL of 2-CEES in N2 flow). The removal efficiency of the ACFs was evaluated and compared with that of AC. From the results, specific surface areas and total pore volume of the ACF were determined to be 1380–1670 m2/g and 0.61–0.82 cm3/g, respectively. It was also observed that various pore characteristics of ACF were found to be dependent on crystallite structure of each precursor. The break through time (C/C0 = 0.10) was in the order of Oxi-Pan-H-9-2 < Saratoga AC < Rayon-H-9-3 < Pitch-H-9-4. This indicates that 2-CEES adsorption capacity could be a function not only of specific surface area or total pore volume, but also of sub-mesopore volume fraction in the range of 1.5–2.5 nm of adsorbents.

Analysis of Lignans in Acanthopanax sessiliflorus Fruits and Their Fermented Wine by HPLC

Hye-Min Kim,Ju-Sun Kim,Seon-Haeng Cho,Sam-Sik Kang,Dae-Sung Cheoi,Sang-Hyun Lee 한국약용작물학회 2006 한국약용작물학회지 Vol.14 No.5

High performance liquid chromatography (HPLC) was used for the determination of lignans, eleutherosides B and E, in Acanthopanax sessiliflorus fruits and their fermented wine. The lignans were quantified by a reversed-phase system using a gradient of H2O and acetonitrile as a mobile phase within 20 min. The analysis was successfully carried out within 20 min. The contents of eleutherosides Band E as main active principles of Acanthopanax species were measured in A. sessiliflorus fruits (1.15 and 8.49 μg/mg, respectively), their fermented wine (0.45 and 1.33 μg/mg, respectively) and wine residues (no detection).

Subcritical water extraction (SWE) of anthocyanin from blueberries (Vaccinium corymbosum)

Hye-Ji Kang,Seon-Woo Kim,Ha-Un Yoo,Na-Yeon Kim,So-Yoon Yee,Min-Jung Ko,Myong-Soo Chung 한국산업식품공학회 2017 학술대회 및 심포지엄 Vol.2017 No.11

Subcritical water extraction (SWE) is an eco-friendly new extraction technology because it does not contain harmful organic solvent and has high extraction efficiency in a short time compared with conventional extraction methods. Blueberries (Vaccinium corymbosum) are widely known as superfood due to rich source of anthocyanin (malvidin-3-o-galgctoside) and antioxidant activity. In this study, optimal extraction condition of SWE from blueberries was determined and compared with the conventional extraction methods. SWE was carried out using a Dionex Accelerated Solvent Extractor (ASE, Model 350) under conditions of temperatures (110, 130, 150 and 170°C) and times (1, 3, 5 and 10 min). Total anthocyanin of SWE extracts was compared with hot water (60°C, 1 h) extract and pressed juice extract. The total anthocyanin content was determined by pH differential method. Considering both the extraction time and temperature conditions, the highest content of total anthocyanin content was 0.455 mg/g FW Vaccinium corymbosum at 130°C for 3 min. At high temperature and long extraction time, the anthocyanin in the blueberries will undergo thermal degradation due to low stability of anthocyanin at extreme condition. Besides, maximum yield of anthocyanin from blueberries using SWE was about 1.2 and 3.8 times more higher than hot water extract and pressed juice extract, respectively. Therefore, SWE is faster and more efficient method to extract anthocyanin from blueberries than conventional extraction methods. This study shows a possibility of SWE applied to food processing industry.

디지털 음악 및 아날로그 음악이 악력에 미치는 영향 평가

최민지 ( Min Ji Choi ),홍지혜 ( Ji Hye Hong ),송영웅 ( Young Woong Song ) 대구가톨릭대학교 자연과학연구소 2011 자연과학연구논문집 Vol.9 No.1

This study evaluated the effect of the music type (analog and digital) on the grip strength. Five male and five female healthy college students participated in the experiment. The independent variables were music type (analog, digital, no music) and listening time (5 min., 10 min., and 15 min.). The classic LP disc was used as the analog music and the LP was converted to mp3 to make the digital music source. Grip forces were measured every 5 minutes until 15 minutes using Jamar strain guqge. Mean forces and peak forces were used as dependent variables. The analysis of variance showed that there was no difference between analog and digital music (p>0.05). Only, the gender effect was statistically significant (p<0.05). These results should be verified using different types of music (not only classical music) or preferred music, during different listening time duration (>15 minutes).

A novel method for olfactory bulbectomy using photochemically induced lesion

Kang, Hye-Min,Jin, Jizi,Lee, Seungjoo,Ryu, Jonghoon,Park, Chan Lippincott Williams Wilkins, Inc. 2010 NEUROREPORT - Vol.21 No.3

We present the photochemically induced olfactory bulbectomy (P-bulbectomy) as a novel method to ablate the olfactory bulb thus inducing an animal model of depression. The photosensitizer Rose Bengal was injected through the tail vein and then the cool halogen light illuminated the skull region overlying of the olfactory bulb for 10 min. Two weeks after surgery, P-bulbectomy had completely removed olfactory bulb uniformly in all animals. P-bulbectomy induced typical depression-related behaviors such as hyperactivity in the open field test and an enhancement of immobility time and in the forced swimming test. Depression-related neurohistological phenomenon was also seen; reduction of choline-acetyltransferase-positive cell numbers in the medial septum and a decline in cell proliferation in the dentate gyrus of hippocampus. This study shows that P-bulbectomy may be a convenient and reproducible experimental method to produce an animal model of depression.

고혈압 환자에서 Nicardipine과 Esmolol의 병용 투여가 기관내삽관시 혈압과 심박수에 미치는 영향

김혜경,이지향,이상곤,반종석,민병우 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.5

Background: This study was designed to determine the efficacy of a combined use of esmolol and nicardipine for blunting hypertension and tachycardia after tracheal intubation in hypertensive patients. Methods: Forty-five hypertensive patients were randomly divided into three group: group E (esmolol 0.5 mg/kg, n = 15), group N (nicardipine 30㎍/kg, n = 15), group EN (esmolol 0.25 mg/kg, nicardipine 15㎍/kg, n = 15). All patients received midazolam 0.5 mg/kg, and glycopyrrolate 0.2 mg IM for premedication. Fentanyl 1㎍/kg was injected before induction of anesthesia, and then esmolol, nicardipine, or the mixed drugs were administrated as an IV bolus and immediately followed by the induction drugs: thiopental 5 mg/kg, and succinylcholine 1 mg/kg. Endotracheal intubation was performed 90 seconds after injection of the experimental drugs. Thereafter 50% Nitrous Oxide in oxygen and 2 vol % enflurane were inhaled. BP and HR were recorded at the resting state, before fentanyl injection (base {T0}), after injection test drug (T1), after induction (T2), immediately after intubation (T3), 2 min (T4), 3 min (T5), 4 min (T6), and 5 min (T7) after intubation. Results: There was a significant attenuation in SBP, MBP, DBP after tracheal intubation on the nicardipine & mixed groups compared to the esmolol group. HR was significantly lower in the esmolol and mixed groups than in the nicardipine group after tracheal intubation. Conclusions: Combined administration of esmolol (0.25 mg/kg) and nicardipine (15㎍/kg) was effective in attenuating an increase of BP and HR during tracheal intubation in hypertensive patients. (Korean J Anesthesiol 2002; 43: 581~587)