한국인 여성의 자궁경부암에서 PCR을 이용한 Human papillomavirus 아형의 검출에 관한 연구

김향우,전호종,김윤신,장원재,이미숙,이미자 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

The polymerase chain reaction (PCR) was used for detection of human papillomavirus (HPV) in paraffin-embedded tissue. Tissues stored as paraffin blocks were a potential source of DNA for retrospective clinicogenetic analysis. Sections in 6 ㎛ (three or five) thick cut from paraffin blocks of 29 uterine cervix dysplasia, 34 uterine cervix carcinoma in situ, 42 uterine cervix squamous cell carcinoma and 10 uterine cervix metaplasia samples were deparaffinized and incubated at 37℃ for 48hrs. in the lysis solution. Following organic extraction and alcohol precipitation, and extracted DNAs were identified by gel electrophoresis. These DNAs carried out PCR. After repeated cycles of specific oligonucleotideprimed extension of viral DNA with thermophilic DNA polymerase. the type of HPV present was then determined on the basis of the size of the ethidium-bromide stained band visible after agarose gel electrophoresis : for HPV 6 is 263 bp. for HPV 11 is 144 bp, for HPV 16 is 601 bp. and for HPV 18 is 360 bp. This study detected HPV DNA in four cases. which broke down to HPV 16 in two case of uterine cervix carcinoma in situ and two case of uterine cervix squamous cell carcinoma, no HPV type in the remainder. Histologically, HPV types 6, 11 are mainly detected in benign genital warts and lowgrade cervical dysplasias, squamous cell carcinoma frequently contained HPV 16, whereas, HPV 18 was present in adenocarcinoma, adenosquamous cell carcinoma and small cell carcinoma of the cervix. These results indicate that cervical cancers might be clinically influenced by the differences in sutible of the infecting HPV.

膵組織 Protein Methylases 活性에 대한 Nicotine의 影響

李香雨,李秀英 成均館大學校 科學技術硏究所 1986 論文集 Vol.37 No.1

It has been known for some time that tobacco smoke or nicotine affects exocrine function of pancreas. Edmund et al. reported that nicotine inhibited pancreatic secretion stimulated by secretin. Furthermore, Solomon et al. have demonstrated that nicotine inhibits the flow of the juice and bicarbonate output. Recently, Majumdar et al. have investigated that the in vitro effect of nicotine on the synthesis and secretion of proteins in dispersed rat pancreatic acini in viro. It was concluded that nicotine stimulated the secretion of preformed zymogen granules and newly sythesized proteins from dispered rat pancreatic acini in vitro. On the other hand, there are many reports that suggest the relationship between protein methylation and pancreatic secretion. Specifically, protein carboxymethylation is involved in arnylase secretion of parotid gland by isoproterenol as well as in pancreatic enzyme secretion. Therefore, in this study we have investigated the effect of nicotine on the activities of protein methylases as well as on pancreatic enzymes in vitro. The following results were obtained; 1) In the presence of 8mM nicotine the secretion of amylase was greatly stimulated from the isolated pancreatic fragments. however, this stimulatory effect of nicotine was inhibited by 2.5mM of cycloheximide. 2) In the case of protein methylase activity, nicotine exhibited the profound stimulation of protein methylase Ⅱ and Ⅲ activity. In the presence of 8mM nicotine, the protein methylase Ⅱ and Ⅲ were stimulated by 3 and 2 folds, respectively, when compared with the corresponding control. This profound increment were blocked by 2.5mM cycloheximide.

數種動物의 膵組織內 Protein Methylases의 活性度 分布에 관한 硏究

李香雨,韓正煥 成均館大學校 科學技術硏究所 1984 論文集 Vol.35 No.1

Post- translational modification of amino acid residue side-chains in protein has come to be a well known biochemical phenomenon. These reactions include methylation, carboxylation, acetylation, phosphorylation, and hydroxylation. One such side-chain modification of protein, protein methylation is being investigated actively with regard to bacterial chemotaxis, cytochrome c, gene regulation, and carnitine biosynthesis. The methylated amino acids occur in nature in highly specialized proteins. These methylated amino acids are formed by reactions catalyzed by protein methylase Ⅰ, Ⅱ and Ⅲ. In recent studies it was reported that protein carboxymethylation is involved in amylase secretion of parotid gland by isoproterenol. It was also suggested that a small part of the total cellular protein carboxymethylation is directly involved in pancreatic enzyme secretion. On the contrary, other authors reported that there is no relationship between protein carboxymethylation and secretion in pancreas and parotid. In this study we first examined the activities of protein methylase I, II, and III in pancreatic tissues of various animals, such as rat, mouse, guinea pig, rabbit, pig, cow, cat, chicken, and frog. The following results were obtained; 1. The activities of protein methylases were generally high in pancreatic tissues of cat, pig, and rabbit. Specifically, the activity of protein methylase I was very high in pancreatic tissue of cat, but low in the tissue of rat. Bovine pancreas had been shown to be the richest source for protein methylase Ⅱ, while the activity of protein methylase Ⅲ was relatively high in pancreatic tissue of cat. 2. The activities of protein methylase Ⅰ, Ⅱ, and Ⅲ in pancreatic tissue of chicken were generally high. 3. Interestingly, the activities of protein methylase Ⅰ, Ⅱ, and Ⅲ were not detected in pancreatic tissue of frog which is amphibian.

스도쿠를 이용한 문제해결력을 위한 새로운 교수-학습자료

임선우,김향숙 인제대학교 2008 仁濟論叢 Vol.23 No.1

창의적인 문제해결력이란 주어진 문제를 해결할 때 다양한 사고의 영역을 스스로 활용할 수 있으며 끝까지 풀어내는 힘을 의미한다. 본 연구에서는 이런 창의적인 문제해결력을 기르기 위한 한 방법으로서 퍼즐에 대해 논의하고, 퍼즐의 한 종류인 '스도쿠'에 대해 여러 가지 해결방법을 알아본 후, 여러 종류의 활동지를 소개하고 있다. The creative problem-solving-ability is the power of solving problem using various fields of thinking with steadiness. In this research, we examined a puzzle as speical way of enhancing the creative problem-solving-ability. And then we found a variety of solution for a 'sudoku' (a kind of puzzle), and introduced some activity using it.

거대 담낭결석의 내시경적 치료 1예 : 내시경적 유두절개술 후 총수담관으로 하행한 거대 담낭결석 Migrated from the Gallbladder through Cystic Duct after Endoscopic Sphincterotomy

이은우,여향순,임동윤 조선대학교 부설 의학연구소 2000 The Medical Journal of Chosun University Vol.25 No.1

The reason why to migrate a large gallbladder stone into the common bile duct (CBD) remains to be established, but among various reasons the diameter of cystic duct appears to be the most important. None of the gallbladder stones with diameters greater than the cystic duct could be passed through it. However, pre-existing CBD stone increases biliary pressure that consequently dilates the cystic duct system retrogradely, allowing larger stones to migrate into the gut. Furthermore, sphincterotomy may actually prevent formation of gallbladder stone and enhance the removal of pre-existing stones from the gallbladder. We recently have experienced a case of a large CBD stone migrated from the gallbladder through the cystic duct after endoscopic sphincterotomy. The cholangitic symptoms were caused by the stone migrated from gallbladder, but several days later got well. So far any side effects related to this removed stone are not found out since we have successfully extracted a large CBD stone (3.5 x 2cm) with mechanical lithotripsy.

유한요소법을 이용한 승용차 steering column의 3-piece 용접부에 대한 열응력해석

김향우,김옥삼 麗水水産大學校 産業技術硏究所 1996 産業基術硏究所 論文集 Vol.5 No.-

Welding of 3-piece is a important technological method in mechanical engineering. Co₂/MAG(Metal Active Gas) welding means that matal part in steering column of passenger car are joined by melting(with or without a filler material) or that new material is added to a matal part by melting. The welding thermal stresses appear due to a non-uniform temperature field. inhomogeneous material properties, external restraint and volume changes during phase trnsformations. The analysis of thermal stresses may also be possible to avoid some large expensive expriments or experimental parametric studies which can often be made less expensively by numerical simulation. The thermal and mechnical properties of material very with temperature, so that the temperature dependence of material properties must be taken into consideration in the thermal stress analysis of these elements. In this study analysis the thermal stresses distribution of 3-piece welding area in steering column for passenger car using finite element method(FEM). Therefore it calculates the numerical value that can be applied to the optimun design of welding parts and the shapes. The significant results obtained in this study are summerized as follows. At early stage of the cooling, the abrupt thermal stresses gradient has been shown in vicinity of the bottom face of welding area. In the thermal stresses analysis due to temperature gradient and heat shocking maxinum stress was occurred in the top part of welding zone and stresses were distributied from 1950 MPa to -1900 MPa.

새로운 무기 응집제 개발에 따른 특성

최성우,우성훈,윤철종,곽명화,서향미,박승조 동아대학교 환경문제연구소 2000 硏究報告 Vol.23 No.1

This study has carried out the coagulation tests for tannery wastewater including high-level suspended solids and organic matters to determine the optimum pH and coagulant dosage and to characterize several coagulants for coagulation. In case of optimum pH range test, we know that multi-coagulants are not only cost-effective but also applicable from acidic pH range to alkaline pH range widely, while single-coagulant are useful in limited pH range. Removal efficiency for using of the multi-coagulants appeared more efficient than that of single-coagulant. Especially, sulfates(alum-ferric sulfate : AFS & alum-magnesium sulfate : AMS) are more effective than chlorides (alum-ferric chloride : AFC & alum-magnesium chloride AMC). In comparison of residual turbidity related to settling time between alum and alum-ferric sulfate-magnesium sulfate(AFM), it took to 40 minutes using of alum but 5 minutes using of alum-ferric sulfate-magnesium sulfate(AFM) until constant residual turbidity are reached.

주물공장 근로자에서 발생한 국한성 석면폐증 1예

김태우,고동희,김민기,유승원,류향우,한정희,정순희 大韓産業醫學會 2009 대한직업환경의학회지 Vol.21 No.2

배경: 주물공장 근로자에서 발생한 석면폐증의 작업 관련성을 평가하고 그 증례를 보고하고자 한다. 증례: 30년간 주물공장에서 근무한 환자로 인후통, 기침, 노란 가래가 있어 검사한 결과 결핵이 의심되어 치료 하였으며, 치료 중 석면폐증으로 진단되었다. 작업 관련성 평가를 위해 실시한 폐 조직의 조직학적 검사에서 1 cm^(2)당 3개의 석면소체가 발견되었고, 투과전자현미경으로 분석한 석면 함유량은 23.26x10^(6) fibers/g이었다. 결론: 작업환경조사, 폐 조직의 석면 분석 등을 통해 작업 관련성이 높다고 판단하였다. Background: Foundry workers are known to be exposed to silica and have an increased risk of Silicosis. There are also reports that state these foundry workers were associated with asbestos-related change in the X-ray results of the lungs. This report discusses a case of localized asbestosis developed in a foundry worker who worked in 12 Korean foundries. Case report: A 49-year-o1d man who worked at molding and melting for 30 years, Presented with sore throat, cough and yellow sputum for 1 month. From physical and clinical examination, Pulmonary tuberculosis was highly suspicious; therefore, he was medicated for 6 months. After tuberculosis medication, the radiological finding was reactivation of tuberculosis, and an asbestos body was detected at a trans-bronchial lung biopsy. During an operation, operators found multiple palpable masses and bullae 10calized in the right upper lobe (RUL) and performed right upper lobectomy and right middle lobe wedge resection. Using hematoxylin-eosin stain and iron stain, we could detect asbestos bodies by light microscopy. We analyzed the asbestos fiber burden in dry lung tissue by transmission electron microscopy with energy dispersive X-ray spectrometer (TEM-EDX). The fiber type was chrysotile and the fiber burden was 23.26 x 10^(6) fiber/g dry lung. Discussion: This case study of a foundry worker with localized asbestosis is uncommon in Korea. We found an occupational relationship between the foundry worker and localized asbestosis through various investigative techniques such as measurements taken at his working environment, clinical, radiological and pathological examination of the foundry worker and mineralogical examination of the asbestos fiber.

膵臟 組織내 Protein Methylase I 酵素의 精製

李香雨,李晦榮 成均館大學校 科學技術硏究所 1985 論文集 Vol.36 No.1

An enzyme which methylates the guanido group of arginine residues of protein using S-adenosyl-L-methionine as methyl donor has been designated as protein methylase I. Among the enzyme products of this reaction which thus far have been identified are N^G-mono, N^G,N^c-di-, and N^G, N'^G- dimethylarginine. This enzyme is widely distributed in various organs of rat, being especially rich in pancreas of hog. The level of protein methylase I activity was found to closely parallel the rate of cell proliferation. Elevated activity was observed in fetal tissues, continuously dividing HeLa S-3 cell culture, regenerating adult rat liver, and in fast growing Novikoff hepatoma. Protein merhylase I has been purified from calf brain, rat and calf thymus, Krebs II ascites cells and recently a form of protein merhylase I has also been purified from wheat germ, which is the first report of the enzyme being present in the plant kingdom. In the present paper, protein methylase I has been partially purified from hog pancreatic tissue. 1) Protein methylase I has been partially purified about 14-fold from hog pancreatic tissue with 25% yield. 2) Mainly the enzyme was found in cytosolic fraction of pancreatic cells. 3) Main purification steps were homogenation of the tissue, S_3 fraction of cells, 35% ammonium sulfate ppt., and DIEM-cellulose chromatography.

웨이블릿과 GLCM을 이용한 회전에도 강건한 질감 영상 검색 기법

김우생,도덕향 광운대학교 기초과학연구소 2000 기초과학연구소논문집 Vol.29 No.-

본 논문에서는 회전에도 강건한 질감 영상 검색을 위해 Wavelet 변환 영역에서의 보다 효율적인 질감 특징추출 방법을 제안하고, 이 방법을 이용한 영상 검색 시스템을 구현하였다. 영상 검색을 위한 특징추출은 2단계 Wavelet 변환 후 생성된 변환 영역 중 저대역 부밴드에서는 웨이블릿 계수 평균값을, 고대역 부밴드에서는 방향 성분과 같은 각도의 변위 벡터(수평-0°, 수직-90°, 대각-45°)로 구해진 GLCM(Gray Level Co-occurrence Matrix) 을 적용한 Energy 값의 합을 사용하였다. 실험을 통하여 제안하는 방법이 Recall과 Precision에서 기존의 방법들 보다 좋은 결과를 보였다.