야생 팽나무버섯의 생리활성 분석

윤향식 외 한국버섯학회 2010 한국버섯학회지 Vol.8 No.1

충북지역에서 수집한 야생 팽나무버섯의 식품소재로의 가능성을 조사하기 위해 동결 건조한 후 일반성분 및 전자공여능, 총폴리페놀 함량, ACE 저해도와 α -glucosidase 저해도 등 생리활성을 분석하였다. 대조군으로 갈뫼팽이버섯과 4종의 수집 야생 팽나무버섯의 일반성분은 건물중으로 분석하였으며, 조단백질은 17.1~21.3 g/100g, 조지방은 4.1~4.9 g/100g, 탄수화물은 65.9~70.5 g/100g, 조섬유는 7.9~18.1 g/100g, 조회분은 5.7~8.4g/100g 범위를 나타내었다. 전자공여능은 63.35~69.31%로 시료간 큰 차이가 없는 것으로 나타났으며, 총폴리페놀 함량은 9.83에서 11.14 mg/g로 대조구와 유사하였다. ACE 저해도는 대조구인 갈뫼 팽이버섯과 수집종인 CBMFV-02가 각각72.91%, 71.44%로 높은 값을 나타내었으며, α -glucosidase 저해도는 CBMFV-01이 46.55%로 가장 높은 값을 나타내었다. In this study, for the development of useful food materials, the proximate composition(total carbohydrates, crude fiber, crude protein, fat and ash) and biological activities(electron donating activity, total polyphenol contents, ACE inhibitory and □-glucosidase inhibitory effects) were determined in the Garlmoe and the four wild Flammulina velutipes. As a results, the dry matter of mushrooms contained large amounts of carbohydrates, from 65.9 to 70.5 g/100g dry weight. the wild collected Flammulina velutipes proved to be an especially good source of crude fiber(7.9~18.1 g/100 g dry weight). Crude protein, fat, and ash contents of the mushrooms ranged 17.1~21.3, 4.1~5.2, and 5.7~8.4 g/100g dry weight, respectively. The electron donating activity ranged from 63.35 to 69.31% and total polyphenol contents ranged from 9.83 to 11.14 mg/g in these mushrooms. ACE inhibitions were high in Garlmoe and CBMFV-02 strain. □-glucosidase inhibition was the highest in CBMFV-01(46.56%) strain and lowest in Garlmoe(15.91%).

검은비늘버섯의 포장재와 저장온도에 따른 저장 효과

김기식,주선종,윤향식,김민아,박성규,김태수 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3

본 연구는 검은비늘버섯((CBPM-6호)의 선도유지 효과를 알아보기 위해 포장재(Wrap, PE, PP, AF) 및 저장온도(1℃, 3℃, 6℃)을 달리하여 18일간 결과를 다음과 같다. 저장기간 중 포장재별 중량 감모율은 랩 포장시 1℃에서 18일 후 3.17%이었으나, AF필름에서는 1.08%의 낮은 중량 감모율을 나타내었다. 색도는 저장기간이 연장될수록 명도와 황색도는 약간 증가하는 반면 랩 표장에서는 감소하는 경향을 나타내었다. 버섯 저장 전 색깔은 랩 포장에서는 3일, PE, PP, AF포장지에서는 18일까지 유지되었다. 경도는 저장온도가 높을수록 낮아지는 경향이었으며 1℃에서 저장할 때 AF 밀봉 저장이 경도의 변화가 가장 적은 것으로 나타났다. 신선도는 1℃ 저장시 신선도 8을 기준으로 랩 포장은 3일, PE 밀봉은 6일, PP 밀봉은 9일, AF 밀봉으로 15일간 저장시 상품성을 유지할 수 있다. This study was carried out to develop storage method for the keeping freshness with various packing film(Wrap, PE, PP and AF and storage temperature(1, 3 and 6℃) of Pholiota adiposa(CBPM-no6) mushroom. The rate of weight loss packed with Wrap film was 3.17%, but AF film was 1.08% after 18days. As the storage periods, lightness and yellowness of mushroom packed with PE, PP and AF were slighty increased, while Wrap film was decreased. The color of original raw mushroom preserved 3days using Wrap film and 18 dyas using PE, PP and AF. As storage temperature high, the rigidity of mushroom decreased. Rigidity of mushroom packed with AF film was less change compared with the other film. Freshness degree of mushroom(refer to Minamide method) could preserve for 3days using Wrap, 6 days using PE, 9 days using PP and 15days using AF film at 1℃.

생쥐 피부의 창상치유에 관한 형태학적 관찰

이향주,이영호,김홍선,김원식 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.1

The back skin of ICR mice were incised. The skin tissues were obtained from the incised wound on the 1st day, 1st weeks, and 2nd week after incision. And these tissue were fixed in 10% neutral buffered formalin and 2.5% glutaraldehyde solution. And, the formalin-fixed tissues were stained by PAS and alcian blue-safranin for the research of morphological changes of epidermis and the mast cells and granulation tissues of dermis. The tissues fixed in 2.5% glutaraldehyde solution were prepared for scanning electron micros-copy, and observed the crusts of scar surface. The results were as follows. 1. The collagen fibers in he granulation tissues at wound region were increased gradually, and became more dense than normal skin on the 2nd week after incision. 2. The newly-formed epidermis was thicker than that of normal skin at the 1st week, and increased in thickness to the 2nd week after incision gradually. 3. The mast cells were decreased in number than those of normal skin on the 1st day after incision, however, increased than those of normal skin on the 1st week after incision. On the 2nd week, the number of the mast cell were similar to those of normal skin. 4. Mixed mast cells containing both histamine and heparin appeared the granulation tissues on the 1st day and 1st week after incision. 5. In scanning electron microscopy, fibrin network on the scar began to appear on the 1st day, formed in a row on the 1st week. But fibrin network changed into irregular rough crusts on the 2nd week after incision.

순천대학교 사범대학 발전 및 특성화 방안 연구

신란식,신향근,이화자 순천대학교 1996 大學發展硏究論文集 Vol.2 No.-

The purpose of this study was to present development plans and to draw up measures to specialize the College of Education (COE) as a teacher training institute after analyzing the problems with which COE in confronted. The plans and measures are primarily to aim attracting many students with better qualities and to assist them to have teaching jobs under the current system of Examination for Teacher Appointment. This study involved multiple data sources obtained from literature review questinnaire survey, and personal contact with the faculty of COE, Questions in the questionnaire were addressed to ask the faculty´s opinions on a curriculum, a school system in university, a practicum for teacher training, a middle and a high school attached to COE, establishing an in-service teacher training institute in COE, and other measures related to the specialization of COE. The findings of the study indicated that the following suggestions should be realized if COE is to be developed and specialized ; 1. A special selection procedure should be applied to attract students with high qualities, 2. Education for the teaching of subject matters should be reinforced and a new curriculum be developed to meet the needs of COE, 3. Appropriate measures should be devised to carry out substantial teacher training for student teachers, 4. A curriculum committe should be formed and asked to work for the management of the curriculum suitable for the specialization of COE, 5. A multi-major or a minor system should be introduced across the student´s specialization fields, 6. A department needed in COE can be established as a major area under the school system in university, 7. The establishment of a middle and a high school attached to COE should be proposed to the Ministry of Education, 8. An educational research center should be established to investigate into issue related to pro-service and in-service education continually, 9. An institute for in-service teacher training and a community education center should be established to render services to the local community, and 10. A second building of COE should be constructed to be equipped with sufficient educational facilities.

Retinol Palmitate가 연골발생에 미치는 영향에 관한 형태학적 연구

이향주,김수일,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.1

Vitamin A, an isoprenoid which has cyclohexynyl ring chemically, is known to promote the growth and differentiation during development and regeneration, to maintain the state of health and to improve the vision, and is known to be essential in mucous secretion, reproduction and maintenance of epithelium. Recently, the anticarcinogenic and antimutagenic effects of vitamin A and the teratogenic effects were reported, however the mechanism of action of vitamin A was not confirmed thoroughly. In this experiment, the morphological changes after administration of retinol palmitate during the development of hindlimb and differentiation of chondrocytes were observed in the rat fetuses. SpragueDawley rats were used as an experimental animal. The rats were divided into 2 groups : control and vitamin A(retinol palmitate) treated group. In retinol palmitate treated group, 500mg/kg of retinol palmitate(Sigma Chemicals Co.) dissolved in corn oil(Sigma Chemicals Co.) was administered orally with gastric tube once at the day 8.5 of gestation. In the control group, corn oil of equal volume as in retinol palmitate treated group was administered. On the gestation day 12.5, 13.5, 14.5, 15.5 and 16.5, the rats were sacrificed under ether anesthesia, laparotomized and hysterectomized. Under stereoscope the pregnant sacs and the fetuses were observed, and the body weight of each fetus was measured. For the observation of external development of hindlimb, the embryos were stained with Nile blue sulfate, and compared the differences between 2 groups. The histological changes were observed light microscopically after routine processing, and H-E & alcian blue staining, and the ultrastructural changes of chondrocytes observed electron microscopically. The results were as follows ; 1. The survival rate of fetuses was lowered in the retinol palmitate treated group significantly except the day 12.5 of gestation. 2. In the retinol palmitate treated group, the development of hindlimb bud and aggregations of precarti lagenous mesenchymal cell were decreased significantly on the day 12.5 and 13.5 of gestation. 3. Cartilage matrix formation was found initially on the day 13.5 of gestation in control group and on the day 14.5 of gestation in retinol palmitate treated group. 4. Electron microscopic changes of chondrocytes after retinol palmitate administration were as the followings : mitochondrial and IR swelling, decreased filopodia, reduced intercellular space and cartilage matrix, abnormal aggregation of glycogen particles and increased intercellular junctions. According to the above results, it is considered that retinol palmitate has an inhibitory effect on the hindlimb developmnet by reducing the aggregations of precartilagenous mesenchymal cell and chondrocytic structural and metabolic changes.

미술치료가 경도정신지체 학생의 부적응 행동에 미치는 효과

이근매,김향지,조진식 한국정신지체아교육학회 2003 지적장애연구 Vol.5 No.-

연구는 경도정신지체 중학생의 부적응 행동을 개선하여 일상생활의 적응력을 높일 수 있도록 하기위해 미술치료를 실시하여 그 효과를 살펴보고자 한다. 연구의 구체적 목적은 다음과 같다. 첫째, 미술치료가 경도정신지체학생의 부적응 행동에 미치는 효과를 알아본다. 둘째, 회기별 진행과정에서 경도정신지체 학생의 부적응행동 변화 에 미치는 효과를 살펴본다. 연구의 결론은 다음과 같다. 첫째, 다양한 미술치료 프로그램을 실시했을 때 경도정신지체 학생의 부적응 행동이 감소되었다. 둘째, 미술치료 초기에는 연구자의 지시에 잘 따르지 못했고 동료와 잘 어울리지 못했지만, 회기가 진행 될수록 연구자와의 상호작용도 증가되었고 동료관계도 개선되었다. 따라서 미술치료 프로그램은 일선 교육현장에서 정신지체아동의 부적응행동을 긍정적으로 개선하는데 유용하게 활용 될 수 있을 것으로 본다. This research sought to conduct art therapy to mild mentally retarded students in middle school to correct their maladjustment behavior and enhance their adaptability to the routine life. The specific objectives of the research are as follows. First, the research determines the effect of art therapy on the maladjustment behavior of mild mentally retarded students. Second, the research determines the effect on the change in maladjustment behavior of the mentally retarded students in the process of treatment by session. The conclusion of the research is as follows. First, when various art therapy programs were offered, the maladjustment behavior of less serious mentally retarded students lessened. Second, the students could not follow appropriately the researcher's instruction and befriend each other during the initial phase of the art therapy, but with sessions continuing, students increased their interaction with the researcher, and improved relations with their peers. Hence, art therapy programs are considered to be useful in improving the maladjustment behavior of mentally retarded children on the education field.

前腕部 經穴 取穴에서 骨度分寸法과 一夫法의 比較 硏究

박히준,채윤병,차웅석,박종배,이혜정,이향숙,인창식,고형균,김수영,최일환,김강식,문정배,배기태,유경환,육근영,정병주,손인철,임사비나 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-

Background : The cun measurement System, an essential and convenient method in locating acupoints, has been widely used in the practice of acupuncture. However, traditional cun measurement has been criticized for its lack of reliability. Objectives : The purposes of this study are to determine if one cun measured by the directional methods have a consistency with that of proportional methods and to investigate which factors are related with these differences, especially in forearm. Methods : The distance between the elbow crease and the wrist crease of forearm was compared to a reference value of one cun obtained by the directional method. In this method, one cun is one third of the distance between index finger and Small finger of a subject, measured at proximal interphalangeal joint. In addition, to investigate the factors influencing the differences between these two methods, we measured the height and body weight and calculated body mass index (BMI). Finally we analyzed the factors correlated with these lengths by linear regression test. Results : The results showed that one cun obtained by the directional methods were significantly different from one cun by the proportional methods in forearm. It was demonstrated that the length acquired with the directional method was more correlated with body weight and body mass index, while the length obtained by the proportional method was more correlated with the height.

Molecular Cloning and Characterization of a Gene Encoding α-L-Arabinofuranosidasefrom Thermotoga maritima

Hyang-Sik Yoon,Inkyung Keum,Nam Soo Han,Chung Ho Kim 한국식품과학회 2004 Food Science and Biotechnology Vol.13 No.2

Characterization of Isolated Yeast from Fermented Grains of Chinese Liquor

Hyang-Sik Yoon,So Yoon Lee,Ye-Rin Ju,Hye-Jeong Kang,Hyun-Ju Eom,In Jae Kim,Youngho Kim 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

Comparison of D- and L-Lactic Acid Contents in Commercial Kimchi and Sauerkraut

Hyang- Sik Yoon,Yeo-Jin Son,Jin Soo Han,Junsoo Lee,Nam Soo Han 한국식품과학회 2005 Food Science and Biotechnology Vol.14 No.1