The reliability of dual rehabilitative ultrasound imaging measurements for muscle co-activation

( Joohee Hahn ),( Hyun-geun Ha ),( Hwang-jae Lee ),( Seungyeop Lim ),( Wan-hee Lee ) 물리치료재활과학회 2017 Physical therapy rehabilitation science Vol.6 No.3

Objective: The aim of this study was to determine the intra-rater and inter-rater reliability of the dual rehabilitative ultrasound imaging (D-RUSI) when simultaneously measuring muscle thickness changes at rest and during co-contraction of the biceps brachii (BB) and triceps brachii (TB). Design: Cross-sectional study. Methods: This study included 36 healthy participants (23 men, 13 women). The participants sat on a chair in a comfortable position with a cushion placed under their elbow to maintain a 90-degree elbow flexion angle. The muscle thickness of the biceps brachii and triceps brachii was measured twice using the D-RUSI by two examiners during resting and co-contraction states. One week later, the same procedure was performed once again. Results: The intra-class correlation coefficients (ICCs) for intra-rater reliability ranged from 0.887 to 0.989 and the confidence interval was within an acceptable range of 0.778 to 0.994. The standard error of measurement (SEM) values ranged from 0.303 to 0.866 and the minimal detectable change (MDC) values ranged from 0.84 to 2.40. The ICCs for inter-rater reliability ranged from 0.758 to 0.925. The SEM values ranged from 0.702 to 1.486 and the MDC values ranged from 1.95 to 4.12. Conclusions: The use of the D-RUSI of the BB muscle had a very good intra-rater reliability and very good inter-rater reliability at the resting state, and a, good inter-rater reliability at the co-activation state. ICC values showed very good intra-reliability and inter-reliability for the TB muscle. the D-RUSI is a useful tool for simultaneously measuring the thickness of two muscles when the BB is an agonist and the TB is an antagonist during co-activation of the upper arm.

Induction of p53-mediated senescence is essential for the eventual anticancer therapeutic effect of RH1

Joohee Jung,Do Young Song,Jung Jin Hwang,Heon Joo Park,Jung Shin Lee,Si Yeol Song,정성윤,최은경 대한약학회 2019 Archives of Pharmacal Research Vol.42 No.9

RH1 (2, 5-diaziridinyl-3-(hydroxymethy)-6-methyl-1, 4-benzoquinone) is a bioreductive anticancerdrug. The mechanism underlying its therapeutic propertieshas not yet been elucidated. In this study, we aimed todetermine whether RH1 exerts its anticancer effect via p53-mediated apoptosis and senescence in vitro and in vivo. RH1 displayed dose-dependent biphasic effects in vitro,i.e., it induced apoptosis at higher dose and senescence atlower dose accompanied by marked activation of p53. Thus, RH1 primarily induced cell death by apoptosis. Thecytotoxicity of RH1 was inhibited in A549 cells treatedwith the p53-inhibitor pifithrin-a or transfected p53 siRNAand in human colon cancer HCT116 isogenic (p53-/-)cells. At sub-lethal doses of RH1, the cells survived andunderwent senescence. The senescent cells showed flattenedand enlarged morphology, and exhibited blue color insenescence-associated b-galactosidase staining. Thesechanges were found to be related to p53. RH1-inducedsenescence decreased in A549-E6 cells (suppressed p53level) and HCT 116 p53-/- cells. The growth of A549xenograft tumors in nude mice was significantly delayed byintraperitoneal injection of RH1, and senescent cells wereobserved in these xenograft tumors. These results suggestthat the in vivo anticancer therapeutic effect of RH1 ismediated by senescence via p53 activation.

The reliability of dual rehabilitative ultrasound imaging measurements for muscle co-activation

Hahn, Joohee,Ha, Hyun-Geun,Lee, Hwang-Jae,Lim, Seungyeop,Lee, Wan-hee korean Academy of Physical Therapy Rehabilitation 2017 Physical therapy rehabilitation science Vol.6 No.3

Objective: The aim of this study was to determine the intra-rater and inter-rater reliability of the dual rehabilitative ultrasound imaging (D-RUSI) when simultaneously measuring muscle thickness changes at rest and during co-contraction of the biceps brachii (BB) and triceps brachii (TB). Design: Cross-sectional study. Methods: This study included 36 healthy participants (23 men, 13 women). The participants sat on a chair in a comfortable position with a cushion placed under their elbow to maintain a 90-degree elbow flexion angle. The muscle thickness of the biceps brachii and triceps brachii was measured twice using the D-RUSI by two examiners during resting and co-contraction states. One week later, the same procedure was performed once again. Results: The intra-class correlation coefficients (ICCs) for intra-rater reliability ranged from 0.887 to 0.989 and the confidence interval was within an acceptable range of 0.778 to 0.994. The standard error of measurement (SEM) values ranged from 0.303 to 0.866 and the minimal detectable change (MDC) values ranged from 0.84 to 2.40. The ICCs for inter-rater reliability ranged from 0.758 to 0.925. The SEM values ranged from 0.702 to 1.486 and the MDC values ranged from 1.95 to 4.12. Conclusions: The use of the D-RUSI of the BB muscle had a very good intra-rater reliability and very good inter-rater reliability at the resting state, and a, good inter-rater reliability at the co-activation state. ICC values showed very good intra-reliability and inter-reliability for the TB muscle. the D-RUSI is a useful tool for simultaneously measuring the thickness of two muscles when the BB is an agonist and the TB is an antagonist during co-activation of the upper arm.

A cisplatin-incorporated liposome that targets the epidermal growth factor receptor enhances radiotherapeutic efficacy without nephrotoxicity

JUNG, JOOHEE,JEONG, SEONG-YUN,PARK, SEOK SOON,SHIN, SEOL HWA,JU, EUN JIN,CHOI, JINHYANG,PARK, JAESOOK,LEE, JAE HEE,KIM, INKI,SUH, YOUNG-AH,HWANG, JUNG JIN,KURODA, SHUN’ICHI,LEE, JUNG SHIN,SONG, SI YEO Spandidos Publications 2015 International journal of oncology Vol.46 No.3

<P>Radiotherapy (RT) is one of the major modalities for non???small cell lung cancer (NSCLC), but its efficacy is often compromised by cellular resistance caused by various mechanisms including the overexpression of epidermal growth factor receptor (EGFR). Although cis???diamminedichloroplatinum(???) (cisplatin, CDDP) has been well characterized as an effective radiosensitizer, its clinical application is limited by its severe nephrotoxic effects. In our current study, we developed a CDDP???incorporated liposome (LP) conjugated with EGFR antibodies (EGFR:LP???CDDP) and evaluated its potential to radiosensitize EGFR???overexpressing cells without exerting nephrotoxic effects. EGFR:LP???CDDP showed higher cytotoxicity than non???targeting liposomal CDDP (LP???CDDP) in the cells expressing EGFR in vitro. In an A549 cell???derived xenograft tumor mouse model, increased delays in tumor growth were observed in the mice treated with a combination of EGFR:LP???CDDP and radiation. Notably, the EGFR:LP???CDDP???treated animals showed no differences in body weight loss, survival rates of nephrotoxicity compared with untreated control mice. In contrast, the use of CDDP caused lower body weights and poorer survival outcomes accompanied by a significant level of nephrotoxicity [e.g., decreased kidney weight, increased blood urea nitrogen (BUN) and creatinine, and pathological change]. These findings suggest the feasibility of using EGFR:LP???CDDP to radiosensitize cells in a targeted manner without inducing nephrotoxic effects. This compound may therefore have clinical potential as part of a tailored chemoradiotherapy strategy.</P>

Nonlinear oscillations of a sessile drop on a hydrophobic surface induced by ac electrowetting

Lee, Joohee,Park, Jun Kwon,Hong, Jiwoo,Lee, Sang Joon,Kang, Kwan Hyoung,Hwang, Hyung Ju American Physical Society 2014 Physical review. E, Statistical, nonlinear, and so Vol.90 No.3

식품알레르기 관리방안에 관한 사례연구 - 은평구 어린이급식소 사례를 중심으로 -

한주희 ( Joohee Han ),김혜리 ( Hyeri Kim ),김지은 ( Jieun Kim ),황해리 ( Hailee Hwang ),황하얀 ( Hayan Hwang ),강지원 ( Jiwon Kang ),주은서 ( Eunseo Ju ),황혜영 ( Hyeyeong Hwang ),변진영 ( Jinyoung Byun ),최지은 ( Jieun Choi ),박유진 한국급식외식위생학회 2022 급식외식위생학회지 Vol.3 No.2

As the prevalence of food allergies increases, related hazards also increase. Among the victims of accidents, children under the age of 10 accounted for a significant portion, raising the need for management of them. To ensure that children are aware of the risk of food allergy for safe meal service and to prevent problems such as malnutrition and poor growth in growing children, the current status of food allergy is surveyed for teachers and parents of children's catering centers in Eunpyeong-gu, and 1: 1 Specialized projects were carried out to provide customized counseling, educational support, alternative diets, and educational materials. As a result, it was found that the improvement of professionalism of faculty members and parents greatly helped to create an environment in which nutritious meals can be provided safely.

Protective Effect of <i>Cimicifuga heracleifolia</i> Ethanol Extract and Its Constituents against Gastric Injury

Kim, Mijeong,Hwang, In Young,Lee, Je-Hyuk,Son, Kun Ho,Jeong, Choon-Sik,Jung, Joohee The Pharmaceutical Society of Japan 2011 Journal of health science Vol.57 No.3

<P><I>Cimicifuga heracleifolia</I> (CH) ethanol extract and its constituents such as ferulic acid, caffeic acid, 24-epi-7,8-didehydrocimigenol-3-xyloside, and 23-O-acetylshengmanol 3-xyloside were investigated for their abilities to prevent gastric injury. To elucidate their gastric-protective effects, we assessed 1,1-diphenyl-2-picrylhydrazyl radical-radical scavenging activity and inhibition of <I>Helicobacter pylori</I> (<I>H. pylori</I>) and gastric cancer cells. Ferulic acid and caffeic acid exhibited higher free radical scavenging activity than other constituents and inhibited the colonization of <I>H. pylori</I> effectively. Furthermore, 24-epi-7,8-didehydrocimigenol-3-xyloside and 23-O-acetylshengmanol-3-xyloside showed cytotoxicity in gastric cancer cells (SNU638 and AGS cells). These results suggest the novel activities of CH ethanol extract and its constituents. CH ethanol extract and its constituents could be utilized for the treatment and/or prevention of gastric injury.</P>

Clinical Significance of Four Molecular Subtypes of Gastric Cancer Identified by The Cancer Genome Atlas Project

Sohn, Bo Hwa,Hwang, Jun-Eul,Jang, Hee-Jin,Lee, Hyun-Sung,Oh, Sang Cheul,Shim, Jae-Jun,Lee, Keun-Wook,Kim, Eui Hyun,Yim, Sun Young,Lee, Sang Ho,Cheong, Jae-Ho,Jeong, Woojin,Cho, Jae Yong,Kim, Joohee,Ch American Association for Cancer Research 2017 Clinical Cancer Research Vol.23 No.15

<P><B>Purpose:</B> The Cancer Genome Atlas (TCGA) project recently uncovered four molecular subtypes of gastric cancer: Epstein–Barr virus (EBV), microsatellite instability (MSI), genomically stable (GS), and chromosomal instability (CIN). However, their clinical significances are currently unknown. We aimed to investigate the relationship between subtypes and prognosis of patients with gastric cancer.</P><P><B>Experimental Design:</B> Gene expression data from a TCGA cohort (<I>n</I> = 262) were used to develop a subtype prediction model, and the association of each subtype with survival and benefit from adjuvant chemotherapy was tested in 2 other cohorts (<I>n</I> = 267 and 432). An integrated risk assessment model (TCGA risk score) was also developed.</P><P><B>Results:</B> EBV subtype was associated with the best prognosis, and GS subtype was associated with the worst prognosis. Patients with MSI and CIN subtypes had poorer overall survival than those with EBV subtype but better overall survival than those with GS subtype (<I>P</I> = 0.004 and 0.03 in two cohorts, respectively). In multivariate Cox regression analyses, TCGA risk score was an independent prognostic factor [HR, 1.5; 95% confidence interval (CI), 1.2–1.9; <I>P</I> = 0.001]. Patients with the CIN subtype experienced the greatest benefit from adjuvant chemotherapy (HR, 0.39; 95% CI, 0.16–0.94; <I>P</I> = 0.03) and those with the GS subtype had the least benefit from adjuvant chemotherapy (HR, 0.83; 95% CI, 0.36–1.89; <I>P</I> = 0.65).</P><P><B>Conclusions:</B> Our prediction model successfully stratified patients by survival and adjuvant chemotherapy outcomes. Further development of the prediction model is warranted. <I>Clin Cancer Res; 23(15); 4441–9. ©2017 AACR</I>.</P>

Use of real-time ultrasound imaging for biofeedback of diaphragm motion during normal breathing in healthy subjects

Cho, Ji-Eun,Hwang, Dal-Yeon,Hahn, Joohee,Lee, Wan-Hee korean Academy of Physical Therapy Rehabilitation 2018 Physical therapy rehabilitation science Vol.7 No.3

Objective: To determine if the provision of visual biofeedback using real-time rehabilitative ultrasound imaging (RUSI) enhances the acquisition and retention of diaphragm muscle recruitment during exercise. Design: Two group pretest posttest design. Methods: Thirty healthy subjects were randomly assigned to the verbal feedback group (VG, n=15) or the visual and verbal feedback group (VVG, n=15). The VG performed breathing exercises 10 times with verbal feedback, and the VVG also performed breathing exercises 10 times with verbal feedback and visual feedback with the use of RUSI to measure changes in diaphragm thickness (DT). For DT, the mid-axillary lines between ribs 8 and 9 on both sides were measured in standing, and then the chest wall was perpendicularly illuminated using a linear transducer with the patients in supine to observe the region between rib 8 and 9 and to obtain 2-dimensional images. DT was measured as the distance between the two parallel lines that appeared bright in the middle of the pleura and the peritoneum. After one week, three repetitions (follow-up session) were performed to confirm retention effects. Intra- and between- group percent changes in diaphragm muscle thickness were assessed. Results: In the VVG, the intervention value had a medium effect size compared to the baseline value, but the follow-up value decreased to a small effect size. In the between-group comparisons, during the intervention session, the VVG showed no significant effect on percent change of DT but had a medium effect size compared to the VG (p=0.050, Cohen's d=0.764). During the follow-up session, retention effect did not persist (p=0.311, Cohen's d=0.381). Conclusions: RUSI can be used to provide visual biofeedback and improve performance and retention in the ability to activate the diaphragm muscle in healthy subjects. Future research needs to establish a protocol for respiratory intervention to maintain the effect of diaphragmatic breathing training using RUSI with visual feedback.

Highly Sensitive Ratiometric Fluorescent Detection of Indium(III) Using Fluorescent Probe Based on Phosphoserine as a Receptor

Mehta, Pramod Kumar,Hwang, Gi Won,Park, Joohee,Lee, Keun-Hyeung American Chemical Society 2018 ANALYTICAL CHEMISTRY - Vol.90 No.19

<P>Indium is one of the most widely used scarce metals for manufacturing various electronic devices including notebooks, mobile phones, and PC monitors. Recent studies revealed that indium and its compound could cause several toxicities to human beings and animals. However, there is no report about ratiometric fluorescent detection of In(III) in aqueous solutions. We synthesized a fluorescent probe (<B>1</B>) for In(III) based on a phosphoserine as a receptor with a pyrene fluorophore using solid phase synthesis. <B>1</B> showed highly sensitive ratiometric response to In(III) in purely aqueous solutions by increasing excimer emission intensity at 476 nm with a concomitant decrease in monomer emission intensity at 395 nm. <B>1</B> showed sensitive ratiometric responses to In(III) over a wide range of pH (2 < pH < 8) and exhibited a highly selective ratiometric response to In(III) among 18 tested metal including Al(III) and Ga(III). Job’s plot analysis indicated that <B>1</B> preferred to form a 2:1 complex with In(III) and the binding affinity for In(III) was measured to be 2.3 × 10<SUP>12</SUP> M<SUP>-2</SUP> (<I>R</I><SUP>2</SUP> = 0.989). <B>1</B> showed linear ratiometric responses to nanomolar concentrations (0-750 nM) of In(III) and the detection limit was calculated to be 64 nM (<I>R</I><SUP>2</SUP> = 0.992) in aqueous solution. The binding mode study using NMR, IR, and CD spectroscopies revealed that the phosphate and the amide groups of the receptor of <B>1</B> played an important role for the binding with In(III). Moreover, <B>1</B> was suitable for the ratiometric detection of In(III) in tap water and groundwater. <B>1</B> showed much better detection properties than those of the colorimetric methods using EDTA with Eriochrome black T (EBT) and 4-(2-pyridylazo) resorcinol (PAR) for the detection of In(III) in tap water and groundwater.</P> [FIG OMISSION]</BR>