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Zhao, Fang-Hui,Tiggelaar, Sarah M.,Hu, Shang-Ying,Zhao, Na,Hong, Ying,Niyazi, Mayinuer,Gao, Xiao-Hong,Ju, Li-Rong,Zhang, Li-Qin,Feng, Xiang-Xian,Duan, Xian-Zhi,Song, Xiu-Ling,Wang, Jing,Yang, Yun,Li, Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5
Objectives: To assess knowledge of HPV and attitudes towards HPV vaccination among the general female population, government officials, and healthcare providers in China to assist the development of an effective national HPV vaccination program. Methods: A cross-sectional epidemiologic survey was conducted across 21 urban and rural sites in China using a short questionnaire. 763 government officials, 760 healthcare providers, and 11,681 women aged 15-59 years were included in the final analysis. Data were analyzed using standard descriptive statistics and logistic regression. Results: Knowledge of HPV among the general female population was low; only 24% had heard of HPV. Less than 20% of healthcare providers recognized sexually na$\ddot{i}$ve women as the most appropriate population for HPV vaccination. There was high acceptance of the HPV vaccine for all categories of respondents. Only 6% of women were willing to pay more than US $300 for the vaccine. Conclusions: Aggressive education is necessary to increase knowledge of HPV and its vaccine. Further proof of vaccine safety and efficacy and government subsidies combined with increased awareness could facilitate development and implementation of HPV vaccination in China.
( Wei Bing Zhang ),( Xiao Ling He ),( Hong Na Liu ),( Hui Yuan Guo ),( Fa Zheng Ren ),( Wei Dong Gao ),( Peng Cheng Wen ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8
In this paper, two statistical methods were applied to optimize medium components to improve the production of the milk-clotting enzyme by Bacillus amyloliquefaciens D4. First, wheat bran juice, skim milk powder, and Na2HPO4 were shown to have significant effects on D4 enzyme production using the Plackett?Burman experimental design. Subsequently, an optimal medium was obtained using the Box?Behnken method, which consisted of 3.31 g/l of skim milk powder, 5.0 g/l of sucrose, 0.1 g/l of FeSO4?7H2O, 0.1 g/l of MgSO4?7H2O, 0.1 g/l of MnSO4?2H2O, 0.1 g/l of ZnSO4?7H2O, 1.52 g/l of Na2HPO4, and 172.45 g/l of wheat bran juice. With this optimal medium, the milk-clotting enzyme production was remarkably enhanced. The milk-clotting enzyme activity reached 3,326.7 SU/ml after incubation of 48 h, which was 1.76-fold higher than that of the basic medium, showing that the Plackett?Burman design and Box?Behnken response surface method are effective to optimize medium components, and B. amyloliquefaciens D4 possessed a high rennet-producing capacity in the optimal medium.
Integrated genome sizing (IGS) approach for the parallelization of whole genome analysis
Sona, Peter,Hong, Jong Hui,Lee, Sunho,Kim, Byong Joon,Hong, Woon-Young,Jung, Jongcheol,Kim, Han-Na,Kim, Hyung-Lae,Christopher, David,Herviou, Laurent,Im, Young Hwan,Lee, Kwee-Yum,Kim, Tae Soon,Jung, J BioMed Central 2018 BMC bioinformatics Vol.19 No.1
<P><B>Background</B></P><P>The use of whole genome sequence has increased recently with rapid progression of next-generation sequencing (NGS) technologies. However, storing raw sequence reads to perform large-scale genome analysis pose hardware challenges. Despite advancement in genome analytic platforms, efficient approaches remain relevant especially as applied to the human genome. In this study, an Integrated Genome Sizing (IGS) approach is adopted to speed up multiple whole genome analysis in high-performance computing (HPC) environment. The approach splits a genome (GRCh37) into 630 chunks (fragments) wherein multiple chunks can simultaneously be parallelized for sequence analyses across cohorts.</P><P><B>Results</B></P><P>IGS was integrated on Maha-Fs (HPC) system, to provide the parallelization required to analyze 2504 whole genomes. Using a single reference pilot genome, NA12878, we compared the NGS process time between Maha-Fs (NFS SATA hard disk drive) and SGI-UV300 (solid state drive memory). It was observed that SGI-UV300 was faster, having 32.5 mins of process time, while that of the Maha-Fs was 55.2 mins.</P><P><B>Conclusions</B></P><P>The implementation of IGS can leverage the ability of HPC systems to analyze multiple genomes simultaneously. We believe this approach will accelerate research advancement in personalized genomic medicine. Our method is comparable to the fastest methods for sequence alignment.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (10.1186/s12859-018-2499-1) contains supplementary material, which is available to authorized users.</P>
이뇨제를 사용한 급성 신손상 환자에서 FEUrea의 진단적 유용성
임대훈 ( Dae Hun Lim ),정지민 ( Ji Min Jeong ),오슬현 ( Seul Hyun Oh ),이형철 ( Hyung Chul Lee ),최준석 ( Joon Suk Choi ),김민지 ( Min Jee Kim ),박정우 ( Jeong Woo Park ),배은희 ( Eun Hui Bae ),마성권 ( Seong Kwon Ma ),김남호 ( Na 대한신장학회 2009 Kidney Research and Clinical Practice Vol.28 No.3
목적: 임상적으로 FENa가 일과성 신손상 (T-AKI)과 지속성 신손상 (P-AKI)을 감별하는 데 많이 사용되지만 이뇨제를 사용한 환자에서 FENa는 유효 혈류량 결핍 상태에서도 증가하게 되어 진단적 정확성이 떨어진다고 알려져 있다. 본 연구에서는 이뇨제 투여상황에서 FE(Na)와 비교하여 FE(Urea)의 진단적 유용성에 대하여 알아보고자 하였다. 방법: 107명의 급성 신손상 환자를 임상적 특성에 따라 일과성과 지속성 신손상 군으로 나누고 이를 다시 이뇨제 투여 유무에 따라 재분류하였다. ROC 곡선에 따라 계산된 cutoff value에 따라서 일과성 신손상을 정의하였고 이뇨제 투여로 인한 cutoff value의 변화에 따른 민감도와 특이도를 비교하였다. 결과: AKI가 발생한 107명의 모든 환자중 검사 이전에 경험적으로 이뇨제를 사용한 경우가 67명으로 63%를 보였고, 일과성 신손상군의 경우는 52명 중 27명으로 52%를, 지속성 신손상군의 경우는 55명 중 45명으로 73%를 보였다. ROC curve에 따라 cutoff value를 FE(Na)≤1.5 FE(Urea)≤30으로 하였을 때 T-AKI를 진단하는 데 사용된 두 값의 민감도와 특이도는 모든 환자군에서 FE(Na)가 81%, 98%를, FE(Urea) 가 94%, 82%를 보였다. 이뇨제 비투여군에서는 FE(Na)가 96 %, 100%를, FEUrea가 92%, 87%를 보였으며, 이뇨제 투여군에서는 FE(Na)가 63%, 98%를, FE(Urea)가 96%, 83%를 보였다. 결론: 이뇨제를 사용한 경우 P-AKI를 진단하는데 FE(Urea)도 FE(Na) 정도의 진단적 유용성을 가진다. Purpose: Although fractional excretion of sodium (FE(Na)) has been used to distinguish transient-acute kidney injury (T-AKI) from persistent-AKI (P-AKI), the availability of FE(Na) in the diagnosis of T-AKI is reported low in patients with diuretics use. We compared the diagnostic performance of fractional excretion of urea (FE(Urea)) with that of FE(Na) in patients with diuretics use. Methods: One hundred seven AKI patients were classified as having T-AKIor P-AKI according to the clinical context. Each group was again subdivided according to exposure to diuretics. According to the cut off value generated by receiver operating characteristic (ROC) curves, sensitivity and specificity of FE(Na) and FE(Urea) were compared with each other. Results: The numbers of patients administered with diuretics were 67 out of total 107 AKI patients (63%), 27 out of 52 (52%) of T-AKI patients, and 40 out of total (65) 55 (73%) of P-AKI patients. When the cutoff value of T-AKI was defined as FE(Na) ≤1.5 and FE(Urea) ≤30 according to the ROC curves, sensitivity and specificity of FE(Na) were 96% and 100% in non-diuretics group, and 63% and 98% in diuretics group, respectively. Sensitivity and specificity of FE(Urea) were 92% and 87% in non-diuretics group, and 96% and 83% in diuretics group, respectively. Conclusion: FE(Urea) is as good as FE(Na) at distinguishing T-AKI from P-AKI in patients administered with diuretics.
Production of an enzyme for treatment of Gaucher's disease using plants
Yoo Na Lee,Ki Seong Ko,Jae Yong Yoo,Bich Ngoc Vu,Young Eun Lee,Ha Na Choi,Mi Hui Jang,Kyun Oh Lee 한국당과학회 2022 한국당과학회 학술대회 Vol.2022 No.07
Gaucher's disease is a metabolic disorder in which a functional deficiency of an enzyme occurs due to a mutation in the gene encoding glucocerebrosidase (GC), resulting in multiple organ malfunctions. Gaucher's disease is being treated with enzyme replacement therapy, which compensates for the enzyme deficiency by administering activated GCs produced in mammalian cells. However, there is a potential of contamination by pathogenic viruses or prions in the mammalian cell-based manufacturing method, as well as a high production cost. In this study, GCs were expressed in plants that produce customized N-glycans to overcome the limitations of the mammalian cell-based production system and to produce a safe treatment drug for Gaucher's disease. A human cDNA library was used to clone a region expressing a GC gene, and sequence analysis was performed. In addition, a binary vector was created and introduced into plants using Agrobacterium to insert the sequence-confirmed GC gene. Plants into which the GC gene was introduced were selected using an antibiotic-containing media, and the presence or absence of the GC gene introduction into the transformed plants was confirmed using polymerase chain reaction (PCR). Furthermore, proteins were collected from the plant leaves into which the gene was inserted, and GC expression levels in the transformed plants were measured using a GC antibody. Finally, a single cloned plant was selected through the antibiotic-resistant isolate ratio. These findings suggest that employing plants, it is possible to overcome the disadvantages of the present mammalian cell-based manufacturing method, decreasing the production cost of pricey Gaucher's disease therapy, and effectively generating safer enzyme treatment.
Han-Na YU,Hye-Jin JO,Ye-Na KIM,Hui-Jin CHEON,Jun-Hong KIM,Jeong-Sun KIM,Jin-Byung PARK 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Ethylene glycol (EG) is a bulk chemical that is mainly used as an anti-freezing agent and a raw material in the synthesis of plastics. Production of commercial EG mostly relies on chemical synthesis using fossil resources. Biochemical production of EG from renewable resources may be more sustainable. Herein, we have examined a strategy for the biosynthesis of EG from formaldehyde. First, we selected glyoxylate carboligase from Escherichia coli K-12 (EcGCL) as glycolaldehyde synthase. EcGCL catalyzes the condensation of two molecules formaldehyde into glycolaldehyde with high thermostability. The variants were constructed to enhance the catalytic efficiency by engineering active site and substrate entrance site based on crystal structures and substrate docking simulation. The best variants (i.g., EcGCL_R484M/N283Q/L478M) exhibited catalytic efficiency (kcat/KM) of 5.2 M<SUP>−1</SUP>·s<SUP>−1</SUP>. This value is ca. three-fold greater than that of the EcGCL wild type. Second, we have chosen (S)-1,2-propanediol oxidoreductase (FucO) from E. coli for the production of EG from glycolaldehyde. The catalytic efficiency of FucO for formaldehyde and glycolaldehyde as substrate showed that 0.05 and 10 mM<SUP>-1</SUP>·s<SUP>-1</SUP> respectively. Using the recombinant E. coli co-expressing EcGCL and FucO, EG was produced to a concentration of 0.41 g/L (6.6 mM) from 20 mM formaldehyde. The conversion reached ca. 66%. The presented studies provide the basis for a new strategy for the production of high value C2 chemicals from C1 chemicals