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Hui Li,Huaibo Li,Yan Bai,Jing Wang,Ming Nie,Bo Li,Ming Xiao 한국미생물학회 2011 The journal of microbiology Vol.49 No.6
Sclerotinia stem rot (SSR) caused by the fungus Sclerotinia sclerotiorum has been an increasing threat to oilseed rape (Brassica napus L.) cultivation. Efficient and environment‐friendly treatments are much needed. Here we focus on microbial control. The Pseudomonas fluorescens P13 that was isolated from oilseed rape cultivation soil, proved to be a useful biocontrol strain for application. Morphology, physiological and biochemical tests and 16S rDNA analysis demonstrated that it was P. fluorescens P13 and that it had a broad antagonistic spectrum, significantly lessening the mycelial growth of S. sclerotiorum by 84.4% and suppressing sclerotial formation by 95‐100%. Scanning electron microscopy studies attested that P13 deformed S. sclerotiorum mycelia when they were cultured together. P13 did not produce chitinase but did produce hydrogen cyanide (HCN) which was likely one of the antagonistic mechanisms. The density of P13 remained at a high level (≥10^6 CFU/ml) during 5 weeks in the rhizosphere soil and roots. P13 reduced SSR severity at least by 59% in field studies and also promoted seedling growth (p<0.05) at the seedling stage. From these data, our work provided evidence that P13 could be a good alternative biological resource for biocontrol of S. sclerotiorum.
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Process optimization for selective hydrogenation of α-pinene over Ni/AlPO4
Chen Yang,Lihong Jiang,Huaibo Wang,Yane Zheng,Yaming Wang 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.2
A new supported Ni/AlPO4 catalyst was synthesized and studied for the selective hydrogenation of α- pinene to prepare cis-pinane. The support was flaky morphology with orthorhombic phase and Ni was well dispersed. The surface area of the catalyst was 37.62m2·g−1 with a pore size of 2.83 nm. For the hydrogenation reaction, the performance of the catalyst was positively correlated with the surface area of support and loading content of Ni. Effects of hydrogenation condition were determined and the process was optimized by response surface methodology. The result suggested that the conversion was positively correlated to hydrogenation temperature, duration and catalyst dosage, while the selectivity showed a negative correlation to temperature and catalyst dosage. After optimization, 95.1% of selectivity was obtained under 94.8% of conversion at 405 K, 81 min and 2.28 wt% of catalyst.
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