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Transcriptional profiles of laccase genes in the brown rot fungus Postia placenta MAD-R-698
Hongde An,Dongsheng Wei,Tingting Xiao 한국미생물학회 2015 The journal of microbiology Vol.53 No.9
One of the laccase isoforms in the brown rot fungus Postia placenta is thought to contribute to the production of hydroxyl radicals, which play an important role in lignocellulose degradation. However, the presence of at least two laccase isoforms in this fungus makes it difficult to understand the details of this mechanism. In this study, we systematically investigated the transcriptional patterns of two laccase genes, Pplcc1 and Pplcc2, by quantitative PCR (qPCR) to better understand the mechanism. The qPCR results showed that neither of the two genes was expressed constitutively throughout growth in liquid culture or during the degradation of a woody substrate. Transcription of Pplcc1 was upregulated under nitrogen depletion and in response to a high concentration of copper in liquid culture, and during the initial colonization of intact aspen wafer. However, it was subject to catabolite repression by a high concentration of glucose. Transcription of Pplcc2 was upregulated by stresses caused by ferulic acid, 2, 6-dimethylbenzoic acid, and ethanol, and under osmotic stress in liquid culture. However, the transcription of Pplcc2 was downregulated upon contact with the woody substrate in solid culture. These results indicate that Pplcc1 and Pplcc2 are differentially regulated in liquid and solid cultures. Pplcc1 seems to play the major role in producing hydroxyl radicals and Pplcc2 in the stress response during the degradation of a woody substrate.
Studies on structure and Color of the White Antberaea pernyi, Silkworm Cocoon
Ji Tao,Liu Hongde 한국생약학회 1995 Natural Product Sciences Vol.1 No.1
Traditional Antheraea pernyi silkworm cocoon is tawny color. The white Antheraea pernyi silkworm cocoons studied here by IR and UV are new breeds of recent breeding. We discovered that the main body of the first grade structure of silk protein in the new white is as same as the one in the traditional tawny Antheraea pernyi silkworm cocoon, yet there are small quantities of cross-linked polymer of gentisic acid and silk protein in the white silkworm cocoons. In spite of the least surviving quantity of the polymer, the white silkworm cocoons are most white and its white color is similar to white Bombyx mori silkworm cocoon.
The Yin-Yang of DNA Damage Response: Roles in Tumorigenesis and Cellular Senescence
Li, Xiaoman,Xu, Hongde,Xu, Chongan,Lin, Meina,Song, Xiaoyu,Yi, Fei,Feng, Yanling,Coughlan, Kathleen A.,Cho, William Chi-shing,Kim, Sang Soo,Cao, Liu MDPI 2013 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.14 No.2
<P>Senescent cells are relatively stable, lacking proliferation capacity yet retaining metabolic activity. In contrast, cancer cells are rather invasive and devastating, with uncontrolled proliferative capacity and resistance to cell death signals. Although tumorigenesis and cellular senescence are seemingly opposite pathological events, they are actually driven by a unified mechanism: DNA damage. Integrity of the DNA damage response (DDR) network can impose a tumorigenesis barrier by navigating abnormal cells to cellular senescence. Compromise of DDR, possibly due to the inactivation of DDR components, may prevent cellular senescence but at the expense of tumor formation. Here we provide an overview of the fundamental role of DDR in tumorigenesis and cellular senescence, under the light of the Yin-Yang concept of Chinese philosophy. Emphasis is placed on discussing DDR outcome in the light of <I>in vivo</I> models. This information is critical as it can help make better decisions for clinical treatments of cancer patients.</P>
Lu, Yuting,Chen, Luyang,Li, Yuze,Xie, Hongde,Cheng, Han,Jin Seo, Hyo Elsevier 2016 Materials letters Vol.164 No.-
<P><B>Abstract</B></P> <P>The work reports the large-scale synthesis and photocatalytic activity of Bi<SUB>23</SUB>V<SUB>4</SUB>O<SUB>44.5</SUB> nanoplates. The structural refinement was conducted in the layered δ-Bi<SUB>2</SUB>O<SUB>3</SUB> based superstructuLre. The detailed surface properties were characterized. Bi<SUB>23</SUB>V<SUB>4</SUB>O<SUB>44.5</SUB> shows efficient absorption in visible region with a band-gap of 2.384eV from the hybridization of Bi-6s and O-2p orbitals. Particularly, Bi<SUB>23</SUB>V<SUB>4</SUB>O<SUB>44.5</SUB> shows an efficient photodegradation for methylene blue (MB). The photocatalysis benefits from the layered B–O structure characteristic and the codoped defects of V<SUP>4+</SUP>/V<SUP>5+</SUP> ions in the lattices. Bi<SUB>23</SUB>V<SUB>4</SUB>O<SUB>44.5</SUB> nanoplates could be a potential photocatalyst.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bi<SUB>23</SUB>V<SUB>4</SUB>O<SUB>44.5</SUB> nanoplates were prepared by the Pechini method. </LI> <LI> It has a narrow band-gap of 2.384eV from hybridization of Bi-6s and O-2p. </LI> <LI> Bi<SUB>23</SUB>V<SUB>4</SUB>O<SUB>44.5</SUB> has photocatalysis report visible-light-irradiation. </LI> <LI> Photocatalysis is due to layered δ-Bi<SUB>2</SUB>O<SUB>3</SUB>-like structure and codoped V<SUP>4+</SUP>/V<SUP>5+</SUP> ions. </LI> </UL> </P>
Xu, Lei,Qin, Chuanxiang,Wan, Yingpeng,Xie, Hongde,Huang, Yanlin,Qin, Lin,Seo, Hyo Jin Elsevier 2017 JOURNAL- TAIWAN INSTITUTE OF CHEMICAL ENGINEERS Vol.71 No.-
<P><B>Abstract</B></P> <P> <I>A</I> <SUB>2</SUB>NiWO<SUB>6</SUB> (<I>A</I> =Ca, Sr) double perovskite semiconductors were prepared via the sol–gel method. The crystal phases were confirmed via X-ray powder diffraction (XRD) measurements together with Rietveld refinements. The morphological and chemical properties of the samples were investigated by the scanning electron microscope (SEM), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX), and the specific surface area measurements. The optical absorptions and the band structures of the two double perovskites were discussed based on the on the experimental results and theoretical calculation. The d–d allowed transitions in Ni<SUP>2</SUP> <SUP>+</SUP>–O octahedra have great contributions to the narrow band-gap. The Ni<SUP>2+</SUP>-containing tungstate perovskites were potential photocatalysts, which are efficient for its photodegradation for methylene blue (MB) under the irradiation of visible light (λ<ce:italic/>> 420nm). The photocatalytic activities were attributed to the special structure properties and multivalent Ni and W ions in the perovskite lattices.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>A</I> <SUB>2</SUB>NiWO<SUB>6</SUB> (<I>A</I> =Ca, Sr) nano-perovskite were prepared via the sol–gel method. </LI> <LI> The crystalline crystal structure was refined. </LI> <LI> The optical absorption and band structure were clarified. </LI> <LI> The photocatalysis activities were discussed on structural properties. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Peiqing La,Xin Guo,Hongding Wang,Ting Shi,Xiaojuan Zhen,Fuan Wei,Xuefeng Lu 대한금속·재료학회 2016 METALS AND MATERIALS International Vol.22 No.2
Bulk 1020 carbon steel was prepared by aluminothermic reaction casting. After casting, isothermal aging treatments at different temperatures are performed for different periods up to 8 h. Microstructure characterization was performed using many methods, including optical microscopy, X-ray diffraction, scanning electron microscopy and transmission electron microscopy. It was found that the steel consisted of a nanocrystalline- ferrite matrix and a microcrystalline pearlite phase with a laminar structure. The average grain sizes of the ferrite were 23, 24, 28, and 37 nm for the cast steel and for samples annealed at 600, 800, and 1000 °C, respectively. As the annealing temperature increased, the volume fraction of the pearlite initially increased and then decreased, while the laminar spacing of pearlite increased from 240 to 900 nm. When annealed at 1000 °C, a spherical black micron pearlite particle was formed. The tensile and yield strength dramatically decreased, and the elongation varied slightly with the annealing temperature. A ductile phase was achieved by extending the holding time.