Effect of Cooking Methods and Lifestyle on Elementary School Children’s Preference for Vegetables Provided in School Lunches

Hong-mie Lee 한국식품영양과학회 2002 Preventive Nutrition and Food Science Vol.7 No.2

Food preference of children may be determined by various lifestyle and environmental factors. This study evaluated the effect of rural or urban inhabitance, cooking methods, and frequency of exposure to vegetable dishes on the acceptance of vegetables by 452 fifth grade children. The subjects were students utilizing meal service in public elementary schools in Boryeoung-city, Chungcheong-nam-do (urban); Cheolwon-gun, Gangwondo (rural); or Pocheon-gun, Kyeonggi-do (suburban). A survey was used to determine the preference of students for 59 foods containing vegetables as a primary ingredient, and categorized by fat content (high, low, or intermediate). Children with working mothers and those living in urban areas tended to have less preference for vegetables than their counterparts in rural areas or with mothers who remained at home. Preference for vegetable foods was correlated with the frequency that vegetable foods were prepared by mothers. Vegetable dishes with a high fat content were preferred over those with low or intermediate fat content, except for soup. This study suggests that children who live in urban areas and whose mothers work outside of the home may be predisposed to nutritional deficiencies due to poor acceptance of vegetable foods, and that all children may be at risk for over-consumption of dietary fat, due to a preference for high fat foods.

Effect of the supplementation of Lycii fructus and taurine to the drinking water on the serum cholesterol level of the rat

Lee, Hong Mie,Jin, Dong Il 선문대학교 ·중소기업기술지원연구소 1999 선문공대 연구/기술 논문집 Vol.4 No.1

구기자액과 타우린용액의 첨가가 포화지방식이를 먹인 흰쥐의 혈청 콜레스테롤 농도에 미치는 영향을 조사하였다 Sprague-Dawley종 4 마리씩을 한 군으로 하여 5 주간 실험식이를 공급하였으며 실험식이는 포화지방의 함량을 증가시키기 위해 시판되는 고형사료에 5%(W/W)의 버터를 첨가하였다 두 실험군은 구기자액(BG)이나 1% 타우린 용액(BT)을 물 대신 공급받았다(BO). 구기자액이나 타우린의 보충은 쥐들의 체중이나 간의 무게에 영향을 미치지 않았다 식이섭취량과 음료수 소비량 또한 구기자액이나 타우린 용액의 보충에 의해 영향을 받지 않은 것으로 나타났다 혈청의 총 콜레스테롤 농도는 구기자액의 보충에 의해 영향을 받지 않았으나 1% 타우린용액의 보충에 의해서는 18% 감소되는 것으로 관찰되었다 The effect of Lycii fructus- and taurine-supplementation to the drinking water on the serum cholesterol level of the rats fed with butter-enriched chow diet was examined Three groups of 4 male Sprague-Dawley rats were fed with a butter-enriched chow diet for the period of 5 weeks two experimental groups were provided with Lycii fructus extract (BG) or taurine solution (BT) instead of plain tap water (BO) Lycii fructus- and taurine-supplementation to the drinking water did not affect body weight increase and liver weight Also the consumptions of experimental diet and drinking solution were not affected by the supplementation of Lycii fructus or taurine The concentration of total serum cholesterol was not changed by the supplementation of Lycii fructus but it was decreased due to the supplementation of taurine by 18%.

마카(Lepidium meyenii)보충이 생쥐에서 Scopolamine으로 손상된 기억력 회복에 미치는 효과

이홍미 ( Hong Mie Lee ),박은진 ( Eun Jin Park ),전인숙 ( In Sook Jeon ),강용수 ( Yong Soo Kang ),진동일 ( Dong Il Jin ),정해정 ( Hai Jung Chung ) 한국식품영양학회 2010 韓國食品營養學會誌 Vol.23 No.4

With an increase in the number of people suffering from ageing-related diseases in our rapidly aging society, interests in natural products such as maca(Lepidium meyenii), which has properties of enhancing cognition and sexual performance, have increased. This study was conducted to assess the effects of 7 weeks of maca extract supplementation(0.5~2.0g/㎏ BW) on scopolamine-induced amnesia in mice and on sperm count in male mice. All doses of maca supplementation significantly protected against scopolamine-induced amnesia as determined by a Morris water maze, but not according to passive avoidance tests. Maca supplementation did not affect acetylcholinesterase activity in the whole brain, nor the testicular sperm count of male mice. This study suggests that maca may have some neuroprotective properties in mice, which will be further examined by future studies.

Studies on the Purification and Partial Characterization of Cysteinesulfinic Acid Decarboxylase from Porcine Liver

Lee, Hong-Mie,Jones, Evan E. Korean Society for Biochemistry and Molecular Biol 1996 Journal of biochemistry and molecular biology Vol.29 No.4

Porcine liver cysteinesulfinic acid decarboxylase was purified approximately 460-fold by means of ammonium sulfate fractionation and sequential column chromatographic separation with Sephadex G-100, DEAE-cellulose and hydroxylapatite. The enzyme has a flat pH profile with maximum activity occurring between pH 6.0 and 7.6. Pyridoxal 5'-phosphate must be present in all buffers used for purification procedures in order to stabilize the enzyme. Addition of sulfhydryl reagents such as 2-mercaptoethanol are also necessary to maintain maximum enzyme activity throughout purification. The absorption spectrum shows that cysteinesulfinic acid decarboxylase is a pyridoxal 5' -phosphate-containing protein. The major absorption is at 280 nm with two smaller absorption regions, one at 425 nm which is ascribed to a Schiffs base between pyridoxal phosphate and protein, and another at 325 nm which is thought to be due to the interaction of 2-mercaptoethanol with the Schiffs base. A number of divalent cations tested did not affect enzyme activity with the exception of mercury, copper, and zinc which are inhibitory. The partially purified enzyme has an apparent $K_m$ of 0.94 mM for cysteinesulfinate. Cysteic acid is a competitive inhibitor of the enzyme with a $K_i$ of 1.32 mM. The molecular weight of the enzyme was estimated to be about 79,600 by using Sephadex G-200 column chromatography.

Effects of Lifestyles, Dietary Habits, Food Preferences and Nutrient Intakes on Sensitivity to and Preference for Salty Tastes of Korean Women

Lee, Hong-Mie The Korean Society of Community Nutrition 2006 Journal of community nutrition Vol.8 No.4

This study was performed to investigate factors affecting sensitivity and preference for salty tastes of Korean adult females. Sensitivity and preference for salty tastes were determined as detection threshold concentration of NaCl solution and the optimally-preferred NaCl concentration of the bean sprout soup, respectively. A self-administered questionnaire was used to obtain the information regarding general characteristics, self diagnosis of stress, health-related lifestyle practices, dietary habits and food preferences. Dietary intake using 24-hours recall and blood pressure were measured. Salty taste detection thresholds and optimally-preferred NaCl concentrations were 0.0197% and 0.357%, respectively. There was a significant positive correlation between the optimally-preferred salt concentration and age, despite no significant correlation between either sensitivity or preference for salty taste and sodium intake, which was 3,605mg/day. Those who had bread or cereal with milk as breakfast instead of a traditional Korean meal and those who preferred jjigae to soup had significantly higher NaCl preferences for bean sprout soup. Going to bed after midnight and skipping meals (${\geq}3/week$) decreased salty taste sensitivity without reaching statistical significance. Self awareness of one's own health, recent weight changes, family history of hypertension, sleep quality, getting-up time, rate of eating and other food preferences did not affect either perception. Stress level, TV watching, BMI and sodium intake did not have significant correlation to sensitivity or preference. Further research including a large number of well-controlled subjects and more accurate measurement of sodium intake should be directed to find other factors affecting salt preference and sensitivity in order to decrease Na intake and related diseases.

Effect of Cooking Methods and Lifestyle on Elementary School Children′s Preference for Vegetables Provided in School Lunches

Lee, Hong-mie The Korean Society of Food Science and Nutrition 2002 Preventive Nutrition and Food Science Vol.7 No.2

Food preference of children may be determined by various lifestyle and environmental factors. This study evaluated the effect of rural or urban inhabitance, cooking methods, and frequency of exposure to vegetable dishes on the acceptance of vegetables by 452 fifth grade children. The subjects were students utilizing meal service in public elementary schools in Boryeoung-city, Chungcheong-nam-do (urban); Cheolwon-gun, Gangwondo (rural); or Pocheon-gun, Kyeonggi-do (suburban). A survey was used to determine the preference of students for 59 foods containing vegetables as a primary ingredient, and categorized by fat content (high, low, or intermediate). Children with working mothers and those living in urban areas tended to have less preference for vegetables than their counterparts in rural areas or with mothers who remained at home. Preference for vegetable foods was correlated with the frequency that vegetable foods were prepared by mothers. Vegetable dishes with a high fat content were preferred over those with low or intermediate fat content, except far soup. This study suggests that children who live in urban areas and whose mothers work outside of the home may be predisposed to nutritional deficiencies due to poor acceptance of vegetable foods, and that all children may be at risk for over-consumption of dietary fat, due to a preference for high fat foods.

Insulin-like activity of taurine

Lee, Hong-mie 대진대학교 1997 大眞論叢 Vol.5 No.-

현대적 의미의 연구가 시작된지 불과 30년 동안 타우린이 관여하는 현상으로 밝혀진 것은 각 분야에서 엄청나게 많이 보고되어지고 있다. 타우린의 작용은 항상성유지 기능, 억제적 기능, 호르몬 유사 기능의 세 가지로 구분되기도 한다. 본 논문에서는 타우린이 인슐린과 같은 작용이 있는지를 확인하고자 하였다. 돼지의 간조직과 골격근 조직에서 방사성 동위원소로 표지된 포도당이 이산화탄소로 산화되는 정도를 측정하였다. 돼지의 골격근 조직에서 방사성 동위원소로 표지된 이산화탄소의 발생량이 타우린의 첨가에 의해 증가되었으므로 타우린이 인슐린 유사 기능을 가지는 것을 시사한다. 그러나 돼지의 간조직에서는 타우린의 첨가가 포도당의 이용 속도에 영향을 미치지 않는 것으로 분석되어 인슐린 유사 기능이 발견되지 않았다. 이 차이가 동물 종간의 차이일 가능성이 있다. 또한 배양액에 타우린과 인슐린을 함께 첨가하였을 때 포도당의 산화 증가가 누가적이지 않았으므로 타우린의 포도당 산화 증가에 대한 기전에 대해서 앞으로의 연구가 필요할 것으로 보인다.

Studies on the Purification and Partial Characterization of Cysteinesulfinic Acid Decarboxylase from Porcine Liver

Lee , Hong Mie,Jones, Evan E 생화학분자생물학회 1981 BMB Reports Vol.29 No.4

Porcine liver cysteinesulfinic acid decarboxylase was purified approximately 460-fold by means of ammonium sulfate fractionation and sequential column chromatographic separation with Sephadex 6100, DEAF-cellulose and hydroxylapatite. The enzyme has a flat pH profile with maximum activity occurring between pH 6.0 and 7.6. Pyridoxal 5'-phosphate must be present in all buffers used for purification procedures in order to stabilize the enzyme. Addition of sulfhydryl reagents such as 2-mercaptoethanol are also necessary to maintain maximum enzyme activity throughout purification. The absorption spectrum shows that cysteinesulfinic acid decarboxylase is a pyridoxal 5'-phosphate-containing protein. The major absorption is at 280 ㎚ with two smaller absorption regions, one at 425 ㎚ which is ascribed to a Schiffs base between pyridoxal phosphate and protein, and another at 325 ㎚ which is thought to be due to the interaction of 2-mercaptoethanol with the Schiffs base. A number of divalent canons tested did not affect enzyme activity with the exception of mercury, copper, and zinc which are inhibitory. The partially purified enzyme has an apparent K_m of 0.94 mM for cysteinesulfinate. Cysteic acid is a competitive inhibitor of the enzyme with a K_i of 1.32 mM. The molecular weight of the enzyme was estimated to be about 79,600 by using Sephadex G-200 column chromatography.

The Body Composition of the Elementary School Children Determined by Segmental Bioelectrical Impedance Analysis (BIA)

Lee, Hong-Mie 대진대학교 1998 大眞論叢 Vol.6 No.-

The body composition of the 542 fifth grade elementary school children residing in Pocheon-Gun was analyzed using the segmental bioelecal impedance analyzer. The subjects were determined to be shorter and lighter than average child in the nation with heights of boys and girls of 143.0±6.1㎝ and 144.3±7.0㎝, respectively and weights of boys and girls of 37.5±8.8㎏ and 37.2±7.8, respectively. Boys were determined to have the significantly shorter stature than girls. Body mass index of the children was 17.9±3.0 and waist hip ratios (WHR) of boys and girls were 84.4±4.2% and 78.1±3.5% respectively. Mineral, protein and fat content of the subjects were 1.84±0.30㎏. 7.18±1.1㎏ and 8.56±4.8㎏, respectively, which consisted in 17.2%, 19.3% and 23.0% of the body weight. The subjects were determined to contain 26.9±4.2㎏ of muscle mass, which consisted in 72.1% of the body weight and 28.7±4.33㎏ of lean body mass, which consisted in 76.9% of the body weight. The children were determined to contain 19.7±3.0l total water and the volume of intra-and extracellular fluid was 13.1±2.0l and 6.42±1.1l. Water content of the right arm, left arm, trunk, right leg, and left leg were 0.832±0.214l, 0.823±0.217l, 8.948±2.434l, 3.060±0.592l, and 3.056±0.587l, respectively.

A Study on the Body Composition according to the Obesity Status of Children aged 10 to 11 Years

Lee, Hong-Mie 대진대학교 1999 大眞論叢 Vol.7 No.-

포천 지역의 초등학교 5학년생 일부를 대상으로 수척군, 정상군, 과체중군 및 비만군별 남녀 아동들의 체수분, 세포내액, 세포외액, 단백질, 무기질, 근육량, 제지방량 및 체지방량을 신체부위별 생체전기저항법으로 측정하여 비교하였다. 남아는 비만군이, 여아는 수척군이 가장 키가 컸고 여아가 남아보다 신장은 유의적으로 크고 WHR은 유의적으로 낮아 이 시기의 여아들이 이미 사춘기에 접어들었음을 시사한다. 남아의 체수분, 세포내외액, 단백질, 무기질, 근육양 및 제지방량은 수척군, 정상군, 과체중군, 비만군으로 비만도가 증가할수록 유의적 증가를 뚜렷이 보이고, 여아의 경우는 남아와 같이 뚜렷하지는 않으나 차례로 증가하여 비만군은 정상군보다 유의적으로 높은 함량을 보였다. 순척군의 이들 체성분함량은 정상군과 비교하여 유의적 차이가 없었다. 세포내액 양은 세포외액 양에 대한 비율은 비만 여아에게서 수척군과 과체중군보다 유의적으로 높게 나타난 것을 제외하고 비만군간 차이가 없었다. 남아 팔, 다리의 수분함량은 정상군, 과체중군, 비만군의 순으로 비만도가 증가할수록 유의적 증가를 뚜렷이 보이고, 여아의 경우 이러한 경향이 팔에서만 보이고 다리에서는 나타나지 않았다. 수척군의 사지와 몸통의 수분함량은 남녀에서 정상군과 비슷했다. 상체허약에 속하는 아동의 수는 비만군, 과체중군, 정상군, 수척군의 순서로 많았으나, 하체허약에 속하는 아동의 수는 비만군별 차이가 없었다. 좌우불균형은 상체에서 하체에서보다 많았고, 여아보다 남아에게서 더 많이 나타났다.