Total oxy-radical scavenging capacity assay를 이용한 taurine과 hypotaurine의 항산화 활성 측정

오정민,주홍매,김상겸,김봉희 忠南大學校 生命科學硏究院 醫藥品開發硏究所 2006 藥學論文集 Vol.21 No.-

Taurine, a sulfur containing non-amino acid, is one of the most abundant intracellular amino acid in humans, and hypotaurine is a precursor of the synthesis of taurine. While it is widely believed that taurine may play an important role in protecting cells against toxic injury by functioning as an antioxidant, there is a lack of evidence to support this hypothesis. In this study, total oxy-radical scavenging capacity(TOSC) assay was used to evaluate antioxidant activity of taurine and hypotaurine against peroxyl radical, hydroxyl radical and peroxynitrite. The present results show that taurine does not have oxy-radical scavenging activity and hypotaurine is comparable to glutathione, an antioxidant substance, in oxy-radical scavenging activity. The results suggest that protective effects of taurine against oxidative stress may not be associated with its direct oxy-radical scavenging activity. Difference in oxy-radical scavenging capacity between taurine and hypotaurine may be ascribed to their sulfur oxidation state.

직업과 생활 습관, 그리고 CYP1A1, GSTM1, GSTT1 유전자 다형성이 요중 1-hydroxypyrene과 2-naphthol 농도에 미치는 영향

김헌,강종원,임현술,이호익,김용대,남홍매,이철호 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.4

Objectives : This study was performed to describe the distribution patterns of urinary 1-hydroxypyrene (1-OHP) and 2-naphthol concentration in coke oven workers and workers not occupationally exposed to polycyclic aromatic hydrocarbons (PAH), and to determine the effects of occupation, life style, and genetic polymorphism of cytochrome P450 1A1(CYP1A1), glutathione S-transferase mu 1 (GSTM1) and theta 1 (GSTT1) on urinary 1-OHP and 2-naphthol concentration. Methods : The study subjects were 19 coke oven workers and 156 shipyard workers. A questionnaire was used to obtain data about detailed smoking and food intake history. Urinary 1-OHP and 2-naphthol concentration and genetic polymorphism of CYP1A1, GSTM1, and GSTT1 were analyzed. Results : The urinary 1-OHP and 2-naphthol concentration was higher in the coke oven workers and in smokers. Urinary 1-OHP concentration was significantly correlated with time after last intake of roasted in non-smoking coke oven workers, whereas urinary 2-naphthol concentration was with amount of cigarette smoking at the sampling day in smoking shipyard workers. Urinary 1-OHP, but not 2-naphthol, concentration of the shipyard workers with Ile/Ile type of CYP1A1 was significantly lower than that of the shipyard workers with other CYP1A1 genotype. Conclusions : Urinary 1-OHP would be a better marker for occupational exposure to PAH in coke oven workers, and urinary 2-naphthol might be better for non-occupational inhalation exposure to PAH. CYP1A1 would not play an important role in the metabolism of naphthalene but in the metabolism of pyrene.

중국거주 조선족 여성의 영적 안녕정도가 정신건강에 미치는 영향

정성덕,이종범,김진성,서완석,배대석,박순재,주열,염형욱,김승원,김구묘,안영록,황대홍,표미자,조창열,정태길 영남대학교 의과대학 2004 Yeungnam University Journal of Medicine Vol.21 No.2

자아초월 정신의학이 체계화되면서 영성(spirituality)은 인성의 한 부분이라는 이론이 대두되었는데 이 영성은 정신건강에 큰 영향을 미친다고 했다. 인간의 성향 중 영적 안녕 정도를 파악하여 그 정도가 정신건강 중 허위성 경향, 정신병적 경향 및 불안과 우울 경향 등에 어떠한 영향을 미치는지를 조사하고저 본 연구를 실시하였다. 대상은 중국의 연변 조선족 자치구에 거주하는 여성 400명을 대상으로 하여 한국판 영적 안녕척도를 사용하여 영적 안녕정도를 파악하였다. 영적 안녕척도의 총점과 이 척도의 2개 하위 척도인 종교적 안녕과 실존적 안녕 척도 양자로 평가한 성적이 불안-우울통합척도와 정신분열증 척도 및 허위성 척도로 평가한 성적에 미치는 영향을 검증하기 위하여 상관관계분석 및 회기분석을 실시하였다. 그 결과는 다음과 같다. 1) 영적 안녕척도로 평가한 조선족 여성의 총점은 68.29로 한국의 기독교 여성이 평가한 100.65보다 훨씬 낮은 점수였다. 2) 불안·우울통합척도로 평가한 총점은 44.88로 연변노인이 평가한 점수와 일개지역의 한국농촌주민이 평가한 점수와 비슷한 결과였다. 3) 허위성 척도로 평가한 성적은 평균 74.57로 70점 이상이 86%(344명)이었으나 영적 안녕 성적과 허위성 성적 간에는 유의한 상관이 없었다. 4) 영적 안녕총점은 정신분열증 척도로 평가한 정신병적 경향에 유의한 영향을 미치지 않았으나 종교적 안녕하위척도의 성적은 정신병적 경향을 높여준데 비하여 실존적 안녕하위척도의 성적은 정신병적 경향을 낮게 해주었다. 5) 영적 안녕척도의 총점 및 두 하위척도인종교적 안녕과 실존적 안녕은 불안과 우울에유의한 상관을 보였는데 영적 안녕총점이 높을수록 불안·우울 총점이 다소 낮아지는 경향을 보였다. 6) 종교적 안녕 하위척도와 불안과 우울과의 관계는 종교적 안녕점수가 높을수록 불안과 우울을 각각 다소 유의하게 높여주었으며 이에 비하여 실존적 안녕하위척도와 불안과 우울과의 관계는 실존적 안녕정도가 높을수록 불안 및 우울점수는 유의하게 낮아졌다. 이와 같은 성적을 미루어볼 때 연변에 거주하는 조선족 여성이 평가한 영적 안녕정도는 정신병적 경향과 불안 및 우울에 유의한 상관을 보였으면 이 척도의 하위 척도인 종교적 안녕정도는 정신건강에 부정적인 영향을 미치는 반면 실존적 안녕정도는 정신건강에 긍정적 영향을 미친 결과로 평가되었다. 이러한 결과는 공산주의 사회에서 실존적 안녕은 긍정적인 가치관으로 평가되는 반면 종교적 안녕은 정신건강에 부정적으로 작용한 것을 입증해 주었다고 하겠다. Background: Spirituality has been an important part of Transpersonal Psychology and is believed to have a large effect on the mental health because it has been systematized. The aim of this study was to determine the level of spiritual disposition on human beings along with its effects on one's mental health. Materials and Methods: The study targeted 400 women residing in Youn-Gil city of JiLin Prov., which is a district of the Cho-Sun tribe in China. Their spiritual well-being was studied using the Spiritual Well-being Scale-Korean Version. The spiritual well-being scale consists of 2 sub-scales of religious well-being and existential well-being. The study was evaluated using a lie scale, psychotic trend, and a combined anxiety-depression scale. The results were considered to be factors of one's mental health. The correlation between the spiritual well-being and each tendency was analyzed by regression analysis. Results: The total score of the Cho-Sun tribal women according to the spiritual well-being scale was 68.29 which was much less than the 100.65 of Korean Christian women. There was no significant correlation between the spiritual well-being and the Lie trend. However, it was found that 86%(344) of Cho-Sun tribal women scored above 70 in the Lie trend with a mean score of 74.57 which is higher than normal populations. Regarding the correlation between the spiritual well-being and psychotic trend, the psychotic trend became significantly higher when the religious well-being was at a high level. On the other hand, the psychotic trend became significantly lower when the existential well-being was at a high level. Regarding the correlation between the spiritual well-being and anxiety, the anxiety was significantly higher when the religious well-being was at a high level. However, the anxiety level was significantly low when the existential well-being was at a high level. Regarding the correlation between the spiritual well-being and depression, the depression level was somewhat significantly high when the religious well-being was at a high level. However, the depression level was significantly low when the existential well-being was at a high level. Conclusion: This study evaluated the effects of spiritual well-being on a person's mental health among Cho-Sun tribal women in Youn-Gil city of JiLIn Prov., P.R. of China. The results found that the religious well-being, which is a sub-scale of spiritual well-being, had negative effects while the existential well-being had positive effects on the mental health. These results proved that a person's religious disposition had negative effects on their mental health in a communitarian society.

Enzymatic synthesis of novel isobavachalcone glucosides via a UDP-glycosyltransferase

Hong-Mei Li,이재경,Li-Juan Nie,Qiang Huo,Tao Ma,송재경,홍영수,Cheng-Zhu Wu 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.12

Glycosylation is often used to improve a natural product’s properties such as water solubility, chemical stability, pharmacological potency, and structure diversification. In this study, we studied the enzymatic synthesis of novel isobavachalcone glucosides using a UDP-glycosyltransferase (YjiC) from Bacillus licheniformis DSM-13. The chemical structures of compounds 1 and 2 were elucidated by spectroscopic techniques, including LC–MS, MS, and NMR. Meanwhile, the parameters of glycosylation reaction such as incubation time, UDP-glucose concentration, and pH of buffer were also optimized during this study. Furthermore, the compounds 1 and 2 exhibited weak anti-proliferative activities against five human cancer cell lines, with IC50 values ranging from 58.6 to 86.6 lM.

A Half Sandwich Ruthenium(II) Complex with N-4,6-Dimethyl-2-pyrimidinylimidazole: Synthesis, Crystal Structure and Application in N-Alkylation of Amines with Alcohols

Hong-Mei Li,Chen Xu,Zhi-Qiang Wang,Wei-Jun Fu 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.10

Analysis ofn Causes of the American Subprime Mortgage Based on Managing Credit Risk

Hong Mei 인하대학교 정석물류통상연구원 2009 인하대학교 정석물류통상연구원 학술대회 Vol.2009 No.10

The mess caused by fast-and loose subprime lending in the U.S. has now blown into a perilous global crisis of credit that has revealed both the scale and the limitation of globalization. Finance is built on trust, and suddenly that trust has been replaced by fear. This will be altogether more testing for the American economy as well as the world"s economies. After this new "prosperity-decline" cycle in the real estate, mortgage and mortgage securities market. The paper systematically summarizes the causes of SMC, and then tries to figure out the relationship between the causes in those different markets. The answer lies in a toxic combination of failures in credit risk management and regulatory oversight, as well as skewed incentives.

Towards Perpetual Peace: Kant and Rawls

( Hong Mei Qu ) 한국윤리학회 2013 倫理硏究 Vol.89 No.1

In 1795, Kant, as a philosopher who never left his hometown published the famous Perpetual Peace which is about the future of all human beings. What dazzled us in the short article are not the concrete strategies for ending war, but the philosophical principles on which a just and lasting world peace can be based. It is this kind of philosophical sketch for perpetual peace that influences us descendants so much. Two hundred years later, Rawls finished The Law of Peoples, in which he expresses the same hope and belief with Kant: perpetual peace for human beings. Rawls himself also indicates that his thought on the principle of the Law of Peoples is based on Kant`s ideal on the world federation.1 However, after reading the two books, I find that Rawls does not follow the train of Kantian thought in the Perpetual Peace as he claims. In order to support this idea, firstly, I refer to Kantian cosmopolitanism which in my opinion is the original mode of all kinds of theories on contemporary global justice. In the second part, I speak of Rawls`s theory on global justice in The Law of Peoples which is in fact describing a hierarchy of different communities and states for us. In the third and fourth parts, I argue that Kantian thought is not only more theoretically far-reaching but also practically viable than Rawls`s by comparing them from the basis of their theories.

Budesonide Microemulsions for Enhancing Solubility and Dissolution Rate

Hong Mei Piao,Hyun Jong Cho,Eui Chaul Oh,Suk Jae Chung,Chang Koo Shim,Dae Duk Kim 韓國藥劑學會 2009 Journal of Pharmaceutical Investigation Vol.39 No.6

Intermedins A and B; New Metabolites from Schisandra propinqua var. intermedia

Hong-Mei Li,Chun Lei,Yong-Ming Luo,Xiao-Nian Li,Xiao-Lei Li,Jian-Xin Pu,San-Yun Zhou,Rong-Tao Li,Han-Dong Sun 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6

A new dibenzocyclooctadiene lignan, intermedin A (1), and a new natural bisabolane sesquiterpenoid, intermedin B (2), were isolated from the aerial parts of Schisandra propinqua var. intermedia. Their structures were elucidated on the basis of extensive spectroscopical analysis.

The Protective Effect of Resveratrol on Dimethylnitrosamine-Induced Liver Fibrosis in Rats

Hong, Sang-Won,Jung, Kyung-Hee,Zheng, Hong-Mei,Lee, Hee-Seung,Suh, Jun-Kyu,Park, In-Suh,Lee, Don-Haeng,Hong, Soon-Sun 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.4

Oxidative stress in liver injury is a major pathogenetic factor in progress of liver fibrosis. Resveratrol, a representative antioxidant derived from grapes, has been reported to show widespread pharmacological properties. In this study, we investigated the protective effects of resveratrol on dimethylnitrosamine (DMN)-induced liver fibrosis in rats. Rats were treated with resveratrol daily by oral gavage for seven days after a single intraperitoneal injection of DMN (40 mg/kg). Resveratrol remarkably recovered body and liver weight loss due to DMNinduced liver fibrosis. Liver histology showed that resveratrol alleviated the infiltration of inflammatory cells and fibrosis of liver tissue. Resveratrol decreased the level of malondialdehyde and increased the levels of glutathione peroxidase and superoxide dismutase. Also, resveratrol significantly inhibited the mRNA expression of inflammatory mediators including inducible nitric oxide, tumor necrosis factor-alpha and interleukin-1beta. In addition, resveratrol showed not only reduced mRNA expression of fibrosis-related genes such as transforming growth factor beta 1, collagen type I, and alpha-smooth muscle actin, but also a significant decrease of hydroxyproline in rats with DMN-induced liver fibrosis. Our results suggest that resveratrol could be used to treat liver injury and fibrosis and be useful in preventing the development of liver fibrosis and cirrhosis.