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Riosmena-Rodriguez, Rafael,Holguin-Acosta, Elena The Korean Society of Phycology 2008 ALGAE Vol.23 No.2
Populations of Codium amplivesiculatum were found in association with rhodolith beds. We present population trends of C. amplivesiculatum from surveys conducted in the southwestern Gulf of California based on monthly sampling from June 1999 to August 2000. Surveys were conducted at 2 sites in the San Lorenzo Channel at depths of 8 - 12 m. The parameters measured were percent cover, biomass and structure in relation to size of the thalli. From each thallus the following measurements were taken: length and diameter, proportion of each thallus with reproductive structures and the degree of fragmentation (measured as number of small thalli). Mean biomass and cover varied seasonally with the maximum (1036.6 gm$^{-2}$ and 100% cover) present in June 2000 and the minimum in winter (0 gm$^{-2}$ and 0% cover). The longest fronds (3 m) occurred during July 1999 and August 2000, while the maximum diameters of 1.0 cm were present during October and November 1999. There were no significant linear relationships between length and diameter of the fronds, suggesting independent growth. Reproductive structures were common in June and July 1999 with monoic and dioic thalli present. Most fronds were monoic, and this is the first record of this feature for this species. Fragmentation occurred in September, and may be associated with stress from early gametangial reproduction and the combination of high temperatures and low nutrients. A hypothetical life cycle for the species is presented.
Impact of Cranberry Juice and Proanthocyanidins on the Ability of Escherichia coli to Form Biofilms
Paola Andrea Pinzón-Arango,Kerrie Holguin,Terri Anne Camesano 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.5
The effects of cranberry juice cocktail (CJC)and proanthocyanidins (PACs) on biofilm formation were investigated. Escherichia coli strain HB101pDC1 and nonfimbriated strain HB101 were grown in 10 wt% CJC or 120 μg/mL PACs for 12 consecutive cultures. Biofilm formation was investigated by incubating bacteria in 96-well polyvinyl chloride (PVC) plates and studying the optical density of the solution using the crystal violet method. We suspect that biofilm formation occurred due to non-specific interactions between the bacteria and the polymer. Both P-fimbriated E. coli HB101pDC1 and the non-fimbriated strain HB101 formed biofilms. E. coli strain HB101pDC1 formed a thicker and more mature biofilm. Cranberry juice inhibited biofilm formation after the first culture; however, for bacteria grown in PACs, a decrease in biofilm formation was observed with increasing number of cultures. The inhibitory effect was reversible. These results demonstrate that CJC is more effective than isolated PACs at preventing biofilm formation, possibly suggesting that other cranberry compounds also play a role in anti-biofilm activity.
Purnama Isti Khaerani,Yunus Musa,Renny Fatmyah Utamy,Wijaya Murti Indriatama,Kannika Umpuch,Alan Alvarez Holguin 한국육종학회 2024 Plant Breeding and Biotechnology Vol.12 No.-
To determine the lethal dose (LD) and growth-reducing dose (GR), the exposures were from gamma activity rates such as low activity rate by multipurpose panoramic 70 Ci and high activity rate by Gamma Cell 3 kCi. The study material was sourced by Cobalt-60 (60Co) with variant doses, i.e., 10Gy, 20Gy, 30Gy, 40Gy, 50Gy, and 60Gy for each gamma activity rate. The study was performed at the Center for Isotope and Radiation Application, National Nuclear Energy Agency of Indonesia (CIRA-NNEA). Data were analyzed using non-parametric tests and analysis of variance. The lethal dose (LD50) and growth reduction (GR50) were identified based on regression analysis. The analysis of variance revealed that highly significant differences among irradiation treatments in number of leaves, survival rate, and plant height. A linear regression model was developed to determine the mean LD50 and GR50 of Super Napier grass. The highest variability of mutants was observed in gamma-ray irradiated mutants with lethal doses (LD50). LD50, 35.82Gy-55.71Gy, at a low activity rate (Multipurpose panoramic irradiator 70 Ci) is higher than the high activity rate (Gamma Cell 3 kCi), 28.98-28.99. In general, the survival rate of Super Napier grass decreased along with increasing irradiation dose. The generated LD50 and GR50 were identified as optimal dosages for the radiosensitivity of Super Napier grass. This study becomes preliminary mutagenesis breeding to generate genetic diversity of grass, specifically in Super Napier grass.