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RISS 인기검색어
- 검색키워드
- 저자 : Hoernig (검색결과 2 건)
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Clonal Analysis of Oral Squamous Cell Carcinoma in Females by Analysis of X-Chromosome Inactivation
Greer, Robert O.,Hoernig, George Korean Academy of Oral Biology and the UCLA Dental 1997 International Journal of Oral Biology Vol.22 No.1
The clonal origin of squamous cell carcinoma of the oral cavity in females was evaluated by analysis of the methylation pattern of the X-linked androgen receptor (HUMARA) gene. DNA extracted from paraffin embedded archival specimens was digested with Hpa Ⅱ, and PCR was performed to generate amplified DNA fragments of exon Ⅰ of the X-linked androgen receptor gene which contains a highly polymorphic trinucleotide repeat. Of a total of 8 tumors analyzed, 7 cases showed heterozygosity (86%) in the length of the amplification products and were therefore informative of clonal analysis. Clonal composition of the tumors was suggested by amplification of a single product band from Hpa Ⅱ treated genomic DNA. By contrast, PCR of Hpa Ⅱ treated DNA from normal tissue or lesional tissue of polyclonal composition resulted in amplified products of both maternally derived and paternally derived X-chromosomes. These observations indicate the clonal composition of squamous carcinoma of the oral mucous membrane in females. We conclude that analysis of the pattern of X-chromosome inactivation, targeting the AR gene by PCR, permits the determination of clonality in a high proportion of cases from archival tissues.
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Telomerase Expression in Precancerous Oral Verrucous Hyperplasia
Greer, Robert O.,George Hoernig,Kenneth Shroyer Korean Academy of Oral Biology and the UCLA Dental 1999 International Journal of Oral Biology Vol.24 No.2
Activation of telomerase and stabilization of telomeres are thought to be required for both cellular immortality and oncogenesis. Telomerase is an essential ribonucleoprotein that maintains telomere length and whose activity is associated with escape from cellular senescenec. Telomeres have been purported to function as mitotic clocks, shortening with age in a replication dependent manner. The role of telomerase in oral dysplastic lesions has not been well defined. The purpose of this study is to investigate the role of telomerase in the precancerous dysplastic lesion verrucous hyperplasia. Using a modifies PCR-based assay for telomerase activity, 23 cases of verrucous hyperplasia were examined using a telomertric repeat amplification protocol (TRAP assay). Telomerase positive controls included enzyme abstracts from HELA cells, a squamous cell carcinoma cell line. Negative controls included RNAse pretreatment of tissue extracts. Fresh tissue samples from 23 cases of verrucous hyperplasia were assayed. Telomerase activity was detectable in 14 of 23 cases. Negative controls which included cheek biting leukoplakias failed to demonstrated telomerase activity. This study suggests that telomerase activation may indeed be associated with a precancerous phase of oral squamous cell carcinoma known as verrucous leukoplakia and that telomerase may serve as a biomarker for cancer risk assessment in patients with certain oral precancers.
RISS
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