랫드 전암 간세포의 증식성과 c-myc, ras의 발현에 관한 연구

조호성,박남용,Cho, Ho-seong,Park, Nam-yong 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.1

This study was carried out to examine proliferative activity and expression of c-myc oncoprotein and p2lras in normal and preneoplastic rat livers induced by an in vivo mid-term chemical carcinogenesis assay. Sixty, six-week-old male specific pathogen free Sprague-Dawley male rats were randomly divided into five groups. Group I was received a single intraperitoneal(IP) dose(200mg/kg) of diethylnitrosamine(DEN). Group 2(10 rats) was operated partial hepatectomy(PH) and Group 3 was received IP(200mg/kg) DEN, fed two weeks later with 500ppm of phenobarbital(PB). Group 4 was received IP(200mg/kg) DEN, fed two weeks later 500ppm(PB) and PH at week 3 after the onset of experiment. While group 5(20 rats) was not treated and used as a control group. All the rats were sacrificed at age 14 weeks except 10 rats from group 5 were sacrificed at the onset of experiment. Livers of all rats were examined for 5-bromo-2'-deoxyuridine(BrdU) incoporation, proliferating cell nuclear antigen(PCNA), silver-binding nucleolar organizer regions(AgNORs) counts per nucleus and expression of c-myc oncoprotein and p21ras. Both the number and area of the preneoplastic lesions were significantly(p<0.01) compared to other groups. A significant(p<0.01) increase in immunoreactive cells were detected in preneoplastic hepatocytes in Groups 3 and 4 by PCNA and BrdU immunohistochemical stain. The number of the positive cells were significantly(p<0.05) lower in normal 14-week-old rats than those of 6-week-old rats. The results showed that proliferative activity of the hepatocytes was increased by treatment with DEN, PH and PB. Meanwhile, AgNORs counts per nucleus were significantly(p<0.05) increased in the preneoplastic hepatocytes of rats in both groups 3 and 4. The expression of c-myc oncoprotein and p21ras were more readily localized within the hepatic preneoplastic lesions such as hyperplastic nodules. Especially, group 4 showed significantly (p<0.05) overexpressed levels compared to groups 1 and 3. These findings suggest that PCNA, BrdU and AgNORs are significantly increased and c-myc oncoprotein and p21ras are significantly overexpressed in hepatic preneoplastic lesions induced by mid-term carcinogenesis. So these parameters can be an effective markers for hepatic prencoplastic lesions.

시험 가축 매몰지 토양 및 침출수 내에서의 구제역 바이러스 검출

조호성 ( Ho Seong Cho ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.4

Foot and mouth disease (FMD) is highly infectious disease of cloven-hoofed animals, particularly cattle, sheep, pigs and goats. Last outbreak reported in November, 2010 induced the enormous social and eco-nomical impacts. Culling of infected animals, movement control, and vaccination are the major control measures of FMD. The aim of this study was to detection foot-and-mouth disease virus (FMDV) in the soil and leachate from modeling burial for pig carcass as measured by real-time reverse transcriptase polymerase chain reaction (RT-PCR). FMDV and Coxsakievirus B1 (CVB1) were detected in soil by week 16 and Coxsakievirus B1 (CVB1) by weeks 12, respectively. FMDV and CVB1 also detected by weeks 8 in the leachate. Results from this study provides an evidence that FMDV could be inactivated for safe of pig carcasses infected with FMDV within 4 month in the carcass burial site.

효율적 자원 배치를 위한 이동객체의 최적 이동패턴 추출

조호성(Ho-Seong Cho),남광우(Kwang-Woo Nam),장민석(Min-Seok Jang),이연식(Yon-Sik Lee) 한국정보통신학회 2021 한국정보통신학회 종합학술대회 논문집 Vol.25 No.2

본 논문은 Fog/Edge Computing(FEC) 환경에서 애플리케이션 서비스의 사용자 근접성을 지원하는 컴퓨팅 리소스의 최적 할당 및 지연시간 감소를 위한 이동에이전트 기반 오프로딩의 효율성을 제고하기 위한 선행연구로써, 이동객체들의 방대한 시공간 이동 이력데이터 집합으로부터 복합적인 시공간 제약을 적용한 최적 이동패턴 추출 시 수행시간 및 소요 메모리양을 효과적으로 감소시키는 알고리즘을 제안한다. 제안 알고리즘은 빈발도 기반의 최적경로 추출을 통하여 향후 FEC 환경에서 계산 오프로딩을 위한 컴퓨팅 리소스의 분배 및 배치에 유용하게 사용될 수 있다. This paper is a prior study to improve the efficiency of offloading based on mobile agents to optimize allocation of computing resources and reduce latency that support user proximity of application services in a Fog/Edge Computing (FEC) environment. We propose an algorithm that effectively reduces the execution time and the amount of memory required when extracting optimal moving patterns from the vast set of spatio-temporal movement history data of moving objects. The proposed algorithm can be useful for the distribution and deployment of computing resources for computation offloading in future FEC environments through frequency-based optimal path extraction.

가축 전염병 관리를 위한 농장 질병관리등급제 개선 방안

조호성 ( Ho-seong Cho ) 한국동물위생학회(구 한국가축위생학회) 2020 韓國家畜衛生學會誌 Vol.43 No.4

The emergence of highly contagious viral diseases of livestock and poultry such as foot-and-mouth disease (FMD), African swine fever (ASF) and highly pathogenic influenza (HPAI) have necessitated the formulation of policies and regulatory frameworks for preventing ingress of exotic diseases and controlling dissemination of endemic diseases within the country. The disease control ratings to manage contagious livestock disease in farms have not being widely used because the evaluation parameters were not being considered inappropriate. The purpose of this study was to reinforce the validity of the parameters for evaluation factors and the objectivity of the scoring criteria in the application of the current disease management rating system. The diseases subject to intensive management were improved by reflecting farm situation. For the evaluation based on the disease grading system of farms, the diseases subject to intensive management were realized, the list of diseases for evaluating the vaccination rate was revised, and the quarantine and sanitation management standards were revised. The effectiveness of the improved livestock disease management system was confirmed through the application of actual pig farms. Therefore, this system will be used as a means to evaluation the biosecurity at the farm units. Through this, it will contribute to establish effective biosecurity measures for each farm by improving and supplementing the present disease grading system.

아시아 물소의 간에 전이된 신장 평활근육종

조호성 ( Ho Seong Cho ),김영섭 ( Yeong Seob Kim ),박남용 ( Nam Yong Park ) 한국임상수의학회 2006 한국임상수의학회지 Vol.23 No.2

가축매몰지 환경관리에 있어 차단방역의 필요 및 절차

조호성 ( Ho Seong Cho ),김건하 ( Geon Ha Kim ) 한국물환경학회 2012 한국물환경학회지 Vol.28 No.2

Avian influenza (AI) and foot and mouth disease (FMD) are two main contagious pathogenic viruses causing massive mortality burial, as burial is a primary measure to quaranteen the causative viruse(s). Biosecurity is a set of preventive measures designed to reduce the risk of propagation of infectious diseases. Main objectives of this paper were to discuss the needs of biosecurity and develop protocol outlines for environmental management of burial sites. Pathological characteristics of contagious viruses should be considered during environmental management practices. Current practice prescribes to minimize the potential for on-farm pollution and the spread of infectious diseases, policy makers should understand robust knowledge regarding biosecurity to make informed decisions on future legislation.

In Situ Hybridization에 의한 돼지 유행성설사증 (Porcine Epidemic Diarrhea)의 진단

박남용,조호성,김태주,박영석,Park, Nam-yong,Cho, Ho-seong,Kim, Tae-ju,Park, Young-seok 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.3

Molecular diagnostic techniques have been used to identify porcine epidemic diarrhea virus (PEDV), a causative agent of acute enteritis in swine, but they were difficult to be petformed and time-consuming. To detect PEDV in a rapid and easy way, we developed biotinylated cDNA probe for N gene encoding the nucleoproteins of PEDV. Formalin-fixed and paraffin-embedded tissues from 24 naturally infected pigs were used for the experiment. The ISH produced a positive reaction in all cases. When intestinal tissues were hybridized with PEDV probe, strong signals were seen in the villus enterocytes of the jejunum and ileum. Hybridization signals were also found in the duodenum from one pig and in colon from dnother. In conclusion, ISH with a biotinylated cDNA probe was provided to be a useful diagnostic method for detecting PEDV effectively in routinely processed tissue sections.

In situ Hybridization에 의한 토끼출혈증(rabbit haemorrhagic disease)의 신속.간편한 진단

박남용,조호성,조경오,김상집,박형선,Park, Nam-Yong,Cho, Ho-Seong,Cho, Kyoung-Oh,Kim, Sang-Jip,Park, Hyung-Seon 한국수의병리학회 2001 한국수의병리학회지 Vol.5 No.2

Recently various molecular diagnostic techniques have been used to identify rabbit hemorrhagic disease virus (RHDV), a causative agent responsible for acute hepatitis and disseminated intravascular coagulation in rabbit. But they were hard to perform and time consuming. To detect RHDV in a rapid and easy way, we developed biotinylated oligonucleotide probe within ORF 1 region encoding the polyprotein of RHDV in formalin-fixed and paraffin-embedded tissues from various tissues of 20 rabbits naturally infected with RHDV, Our in situ hybridization (ISH) was quickly carried out within two hours by MicroProbe capillary action system. The ISH produced a positive reaction in liver, kidney and lung. In conclusion, ISH with a biotintlated oligonucleotide probe provided a useful diagnostic method for detecting RHDV.

소의 임상병리 가검물에서 Mycobacterium species 감별진단을 위한 multiplex PCR 기법

김용환,조호성,강성귀,조경오,박형선,이봉주,박남용,Kim, Yong-hwan,Al-Haddawi, MH,Cho, Ho-seong,Kang, Sung-kwi,Cho, Kyoung-oh,Park, Hyung-seon,Lee, Bong-joo,Park, Nam-yong 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.4

A multiplex PCR technique was developed for detecting specifically each Mycobacterium bovis, M. tuberculosis, M. avium and M. avium subsp, paratuberculosis, respectively, using clinical samples of field cattle. To apply this novel technique to clinical specimens, blood sample was obtained from live cows comprising 11 intradermal tuberculin test (ITT)-positive and 17 ITT-negative and tested by multiplex PCR. Positive results were obtained from 15 cows by the multiplex PCR, showing that 4 (23.5%) of the 17 ITT-negative cows were multiplex PCR positive. The multiplex PCR results also showed that among the 15 positive cows, 7 (46.7%) were infected with M. bovis, 1 (6.7%) with M. tuberculosis and 7 (46.7%) with M. avium. The sensitivity and specificity of multiplex PCR in comparison with those of ITT were 100% and 76.5%. The correlation between the multiplex PCR and ITT assays with blood samples was considered excellent, 85.7% agreement and ${\kappa}=0.72$. The results obtained, using reference mycobacterial strains and typed clinical samples, show that the multiplex PCR method may be a rapid, sensitive, and specific tool for the differential identification of various mycobacterial strains in a single-step assay. Therefore, multiplex PCR assay is a useful tool for early diagnosis of tuberculosis in live cattle and to identify the species or complex of mycobacterium from clinical samples.

In situ RT-PCR 및 In situ hybridization 기법에 의한 닭 뉴캣슬병의 진단법 개발

박남용,최효임,조호성,강성귀,조경오,Park, Nam-Yong,Choi, Hyo-Im,Cho, Ho-Seong,Kang, Sung-Kwi,Cho, Kyoung-Oh,Brown, Corrie 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.3

Newcastle disease (ND) is a highly contagious infection of poultry, Two pathology-based techniques, in situ RT-PCR and in situ hybridization (ISH) were applied to formalin-fixed, paraffin-embedded tissues from chickens naturally infected with velogenic ND virus (VNDV). Two pairs of primers and a probe for ISH and in situ RT-PCR, respectively, were selected from highly conserved region of matrix gene of NDV. The ISH experiment was carried out using MicroProbe$^{TM}$ capillary action system within 2 hours. In situ RT-PCR was performed using MicroProbe$^{TM}$ capillary action system and GeneAmp In Situ PCR system. With ISH and in situ RT-PCR, viral nucleic acid was detected in the central nervous system of chickens from infected with neurotropic velogenic Newcastle disease virus (NVNDV), whereas viral nucleic acid was detected in various organs or tissues of chickens from infected with viscerotropic velogenic Newcastle disease virus (VVNDV). In the NVND group, positive signals were characteristically defined in the cytoplasm of neuron, vascular endothelial cells, and perivascular mononuclear macrophages in the central nervous system. One of NVND group, chicken from one farm exhibited positive signals in the bronchial epithelium. The VVND group chickens showed positive reaction in the macrophages, vascular endothelium, and bronchiolar epithelium. Markedly, viral nucleic acid was detected in the macrophages of morphologically normal tissues which were peripheral or located in distant areas from lesions. The central nervous system of chickens infected with VVND virus had positive signals in the vascular endothelial cell, perivascular mononuclear macrophages and some neuron. The number and intensity of the positive cells by in situ RT-PCR were more and stronger, respectively, in comparison with those by ISH. Particularly, positive reaction was detected in macrophages infiltrating in cardiac muscle by in situ RT-PCR, but not obtained by ISH. Therefore, these results demonstrated that ISH is a rapid diagnostic method for detection of NDV and in situ RT-PCR can be used as an efficient method for detection of low viral load infection or subclinical viral infection of NDV.