한우 myostatin 유전자의 SNP 및 발현분석

유성란,정기철,상병찬,이준헌 충남대학교 농업과학연구소 2004 농업과학연구 Vol.31 No.2

Myostatin is a transforming growth and differentiation factor-β family member that acts as a negative regulator of muscle growth. Previously, mutations in the myostatin gene were known to be related to double muscling phenotypes in cattle. Because myostatin gene is highly related to muscle mass, also meat quality, in cattle, we sequenced whole myostatin mRNA and investigated the SNPs (Single Nucleotide Polymorphisms) in Korean cattle (Hanwoo). The results indicated that Hanwoo had an SNP in nt2385 and this mutation can be a useful marker with further verifications. We also investigated expression patterns of the myostatin gene from various muscle tissues and organs. Northern blotting results indicated that myostatin expression was restricted in muscles with variable expression levels. The results presented here can be used as a valuable information for meat quality related traits and muscle mass in cattle.

配車間題의 發見的 解의 改善에 關한 硏究

宋省憲 弘益大學校 1985 弘大論叢 Vol.17 No.2

Various heuristic algorithms for the traveling salesman, and vehicle routing problems are classified by their peculiar criteria and their interrelations are discussed. An efficient refinement procedure is presented to improve any results by the existing heuristic algorithms which is not optimal.

一般産業廢棄物의 地域連繫에 의한 再活用方案

盧載星,洪性秀,趙憲英 忠南大學校 地域開發硏究所 1991 地域開發論叢 Vol.3 No.-

지역사회 획득 급성 신우신장염 여성환자에서 검출된 원인균의 항균제 감수성 및 항균제의 사용

위성헌,최수미,이동건,김수영,김상일,유진홍,신완식,강문원 대한감염학회 2002 감염 Vol.34 No.6

쇼트피닝 가공한 평기어의 피로특성

정성균,정동건,박충식,김태형,이재헌 서울産業大學校 2002 논문집 Vol.53 No.1

In this paper, the effect of shot peening on the fatigue characteristics have been investigated. Two types of specimens were used to obtain the experimental results. One type of specimen was made by machining and heat-treating SCM415 steel. The other type of specimen was made by machining, heat-treating, and shot peening SCM415 steel. The hydraulic testing machine was used to do the fatigue test (R=0.1, 10Hz). The experimental results show that the fatigue life and strength of shot peened gear were largely improved compared with the unpeened gear.

Staphylococcus aureus와 Coagulase-Negative Staphylococcus Species에 대한 Arbekacin의 시험관내 항균력

위성헌,강진한,허동호,이동건,김상일,김양리,최정현,김종현,유진흥,허재균,신완식,강문원 대한감염학회 2001 감염 Vol.33 No.4

Background : Most strains of methicillin-resistant Staphylococcus aureus (MRSA) now exhibit high-level resistance to various antibiotics, such as β -lactam antibiotics, aminoglycosides, macrolides, tetracyclines and quinolones. Recent reports describing the therapeutic failure of vancomycin for MRSA infections have arisen considerable concerns regarding the emergence of MRSA strains, which will require new therapeutic agents. Arbekacin, an aminoglycoside antibiotic, has antibacterial activity against both gram-positive and gram-negative bacteria and is stable in the presence of aminoglycoside inactivating enzymes produced by S. aureus. In this study, we compared the antibacterial activity of arbekacin with those of vancomycin, gentamicin, and amikacin against Staphylococcus aureus (S. aureus) and coagulase-negative staphylococci (CNS). Methods : For a collection of 549 S. aureus and 251 CNS isolates from three Catholic University Hospitals in Korea, minimum inhibitory concentrations (MICs) of arbekacin, vancomycin, amikacin and gentamicin were determined by agar dilution method using Mueller-Hinton agar according to NCCLS (National Committee for Clinical Laboratory Standards, USA)criteria. Results : Among 549 S. aureus isolates, 278 isolates were MRSA and 271 isolates were methicil sensitive S. aureus (MSSA). MIC50 & MIC90 of arbekacin against 549 S. aureus were 0.5 & 1 ㎍/mL, and MIC50 & MIC90 of vancomycin were 1 & 1 ㎍/mL. MIC of arbekacin against 549 S. aureus isolates ranges from 0.03 to 4 ㎍/mL, and MIC of vancomycin against 549 S. aureus ranges from 0.25 to 2 ㎍/mL. MIC90 of amikacin against 549 S. aureus was 32㎍/mL, and that of gentamicin was 128 ㎍/mL. MICs of amikacin and gentamicin were variable, ranging from 0.125 to 256, and otherwise arbekacin and vancomycin revealed relatively narrow range of MICs. MIC90 of arbekacin against 278 MRSA isolates & 271 MSSA were 1 & 0.5 ㎍/mL, and those of vancomycin against MRSA & MSSA were 1 & 1 ㎍/mL. MIC90 of amikacin against 278 MRSA & 271 MSSA isolates were 32 & 4 ㎍/mL, and that of gentamicin against MRSA & MSSA isolates were 128 & 32 ㎍/mL respectively. Among 251 CNS isolates, 122 isolates were MRCNS and 129 were MSCNS. MICSO & MIC90 of arbekacin against 251 CNS isolates were 0.25 & 2 ㎍/mL, and those of vancomycin were 1 & 2 ㎍/mL. MIC of arbekacin against 251 CNS isolates ranges from 0.015 to 32 ㎍/mL, and that of vancomycin isolates ranges from 0.25 to 2 ㎍/mL, MIC90 of arbekacin against 122 MRCNS & 129 MSCNS isolates were 2&0.3 ㎍/ML, and those of vancomycin were 2&s ㎍/ML. MIC90 of amikacin against 251 CNS isolates was 32 ㎍/ML, and that of gentamicin was 128 ㎍/ML for CNS. MIC90 of amikacin against 122 MRCNS & 129 MSCNS isolates were 128 & 8㎍/mL, and those of gentamicin ere 256 & 32 ㎍/mL. Conclusion : Considering above results, arbekacin can be useful agent against most strains of MRSA and MRCMS, which exhibit high-level resistance to amikacin and gentamicin. (Korea J Infect Dis 33:254~260, 2001)

토끼의 발육에 따라 flecainide가 방실전도에 미치는 전기생리학적 영향

한헌석,김성희 충북대학교 의과대학 충북대학교 의학연구소 1998 忠北醫大學術誌 Vol.8 No.2

연구목적 : 성인의 부정맥에 널리 쓰이는 flecainide는 불응기를 별로 변화시키지 않고 전도를 늦추는 약제이나, 미숙한 심조직에서의 전기생리학적 성질에 관하여는 거의 알려져 있지 않다. 기본심박수가 빠르고 심조직이 미숙한 소아의 부정맥에서는 항부정맥제의 심박수 의존 효과 및 발육에 따른 차이가 임상적으로 중요할 것으로 생각되어 토끼의 관류심장을 이용하여 방실전도에 있어서의 flecainide의 효과를 연구하였다. 대상 및 방법 : 토끼의 관류심장을 이용하여 방실전도에 있어서의 flecainide의 효과를 연구하였다. 표준화된 조기 자극 공정표를 사용하여 성숙토끼와 미숙토끼의 심장에서 방실전도와 불응기를 측정하여 대조상태와 flecainide를 준 상태를 비교하였다. 결과 : (1) AH와 HV 전도를 늦추는 최소 효과용량은 성숙 및 미숙토끼의 심장에서 모두 0.5㎍/ml로 같았다 (2) 양군 모두 방실결절 및 His 하부의 전도에서 심박수 의존효과가 있었다. (3) Flecainide는 급속전도와 완속전도에 같은 효과를 가지고 있어, 양군에서 모두 flecainide의 용량을 증가시킴에 따라 두지표가 같은 정도로 상승하였다. 약물 농도를 증가시킴에 따라 HVmin과 HVmax이 평행하게 증가함으로 His 하부의 회복곡선의 진폭(AHV)에는 변화가 없었다. (4) 신생토끼가 성숙토끼보다 flecainide에 데한 Wenckebach cycle 길이가 더욱 민감하게 반응 하였다. 양판에서 모두 역행성 Wenckebach cycle 길이가 전향성보다 민감하게 반응하였다. 5) 신생토끼의 불응기가 성숙토끼보다 flecainide에 더욱 민감하게 반응하였다. 결론: Flecainide의 심박수의존 및 방향의존성 효과와 미숙한 심장의 불응기가 flecainide에 더욱 민감하게 반응하는 점이 노아의 상실성 및 심실성 부정맥을 조절하는 기전으로 설명할 수 있을 것으로 보인다. Purpose : Flecainide, slowing conduction with little changes in refractory periods, was not widely studied for the electrophysiologic effect in the immature cardiac tissue, though extensively used in adult arrhythmia. The rate-dependent effect and developmental difference are considered to be important in pediatric patients whose tissue is immature and intrinsic heart rate is faster. The authors tried to study the effect of flecainide in atrioventricular conduction using isolated perused rabbit hears. Maternal and Methods : With standard premature pacing protocol, the atrioventricular conduction and the refractory periods were measured in control and graded concentrations of flecainide in isolated perfused adult and neonatal rabbit hearts. Results : (1) The minimal effective concentrations for AH and HV conduction slowing were 0.5㎍/ml in both groups. (2) Both groups had rate-dependent effect in nodal and infra-Hisian conduction. (3) Flecainide had equivalent effects on fast and slow conduction, producing an equivalent upward shift in both parameters with increasing drug in both groups. A parallel enhancement of HVmin and HVmax with increasing drug concentrations produced no change in the amplitude of the infra-Hisian recovery curve. (4) The Wenckebach cycling length of neonate was more sensitive than that of adult, and the retrograde direction was more sensitive than the antegrade direction in both groups. (5) The refractory periods of neonate were more sensitive than those of adult. Conclusion : Conclusively, rate-dependent and direction-dependent effects of flecainide and more sensitive refractory Periods in immature cardiac tissue might explain the effect of flecainide in the pediatric supraventricular and ventricular arrhythmia whose tissue is immature and intrinsic heart rate is faster.

제주도 연안 초망 어선의 소음에 관한 연구

박성욱,서익조,오승훈,김병엽,이창헌,서두옥 濟州大學校 海洋硏究所 2002 해양과환경연구소 연구논문집 Vol.26 No.-

제주도 연안에서 이루어지는 초망 어업에서 수중음을 이용하여 멸치 어군을 유효하게 집어하기 위한 기초 자료를 얻기 위하여 어선의 주기관을 공회전시켰을 때의 선상과 어군이 유집되어 투망되는 수심 4m지점과 함께 수심 10m층의 수중 소음을 측정 조사하였다. 이때 사용한 선박의 공회전은 전진시의 800 rpm과 후진시의 1000 rpm으로 그 결과는 다음과 같다. 1. 기관의 회전수 800 rpm에서 선수(B), 갑판(D), 선미(S), 연돌 위(F), 기관실 내(E)의 소음 준위는 각각 90, 93, 106, 107, 101 dB 이었으며, 기관 회전수 1,000 rpm에서의 소음 준위는 각각 93, 95, 110, 109, 103dB이었다. 2. 회전수를 800rpm으로 할 경우 100, 300, 500, 1000, 1500, 2000 Hz에서 선수의 소음 준위는 4m인 경우 각각 92, 95, 102, 97, 94, 92dB 이었으며, 수심 10m인 경우 87, 88, 99, 96, 94, 93 dB이었고, 선체 중앙에서는 4m인 경우 94, 91, 100, 96, 94, 92dB, 10m인 경우 93, 92, 99, 95, 93, 91dB이었다. 선미에서는 4m인 경우 98, 94, 100, 97, 94, 92 dB이었으며 10 m인 경우 92, 93, 99, 96, 93, 92 dB로 나타났다. 3. 후진시 사용하는 회전수 1000 rpm으로 할 경우 100, 300, 500, 1000, 1500, 2000 Hz에서 선수 소음 준위는 4m인 경우 각각 86, 91, 98, 95, 93, 92 dB로 나타났고, 10m인 경우 88, 87, 99, 96, 94, 92 dB로 나타났으며, 선미에서는 4m인 경우 88, 88, 99, 96, 93, 92 dB, 10m인 경우 88, 86, 99, 96, 94, 92 dB로 나타났다. In order to obtain a basic data for alluring a school of fish, anchovy with emitting underwater sound, an experimnet for measuring noise level was carried out on no-load operation of a engine. noise level was measured at the depths of four meters and ten meters and analyzed. Then, the revolution per minute of the ship used at this field experiment was 800 including 1000. The results were followed. 1. On 800 rpm, noise levels on board were 90, 93, 106, 107 and 101 dB in the order of bow, deck, stern, funnel, funnel and engine room. In addition, 93, 95, 110, 109 and 103 dB on 1000 rpm, respectively. 2. on 800 rpm, underwater noise levels of the depth of four meters at the bow were 92, 95, 102, 97, 94 and 92dB at the frequencies of 100, 300, 500, 1000, 1500 and 2000 Hz, respectively and those of the depth of ten meters were 87, 88, 99, 96, 94 and 93 dB. Underwater noise levels of the depth of four meters outside the engine room were 94, 91, 100, 96, 94 and 92 dB at the frequencies of 100, 300, 500, 1000, 1500 and 2000 Hz, respectively and those of the depth of ten meters were 93, 92, 99, 95, 93 and 91 dB. Underwater noise levels of the depth of four meters at the stern were 98, 94, 100, 97, 94 and 92 dB at the frequencies of 100, 300, 500, 1000, 1500, and 2000 Hz, respectively and those of the depth of ten meters were 92, 93, 99, 96, 93 and 92 dB. 3. On 1000 rpm, underwater noise levels of the depth of four meters at the bow were 86, 91, 98, 95, 93 and 92 dB at the frequencies of 100, 300, 500, 1000, 1500 and 2000 Hz, respectively and those of the depth of ten meters were 88, 87, 99, 96, 94 and 92 dB. Underwater noise levels of the depth of four meters at the stern were 88, 88, 99, 96, 93 and 92 dB at the frequencies of 100, 300, 500, 1000, 1500 and 2000 Hz, respectively and those of the depth of ten meters were 88, 86, 99, 96, 94 and 92 dB.

돈분뇨의 토양처리시 이온 성분의 용탈 특성

김태헌,류성필,김성수,오윤근,허철구 한국환경과학회 2003 한국환경과학회지 Vol.12 No.3

An agricultural land application of swine slurry is one of the best management practices in Jeju island whose ground water must be protected. So as to study the effect of appling swine slurry on ground water or aquifer, incubation-leaching technique was used by assuming the incubating period of 1, 2, 4, 8, 16, or 32 days, and application rate of 3200.0㎎T-P/ℓ, 820.0㎎T-P/ℓ, and 1887.0㎎K^+/ℓ in swine slurry. The leachates were collected from the soil columns(PVC 30㎝L×5.5㎝D) packed 15㎝ in depth with Gangjeong soil series by washing with 100mL distilled water. The leached components were measured by using Ion chromatography for Cl^-, NO_3-N, F^-,Br^-, Na^+, K^+, Ca^2+, and Mg^2+, atomic absorption spectrophotometry for Fe and Mn, and UV-Vis spectrophotometry for T-N and T-P. Application of swine slurry in naked soil could influence on the ground water or aquifer by increasing nitrate-nitrogen in leachate with time, or leaching the cations present in soils in accompany with anions because of H^+ produced in nitrification. Therefore, careful consideration should be taken about what amount, when, where, and how for protecting ground water system.

고정식 교정환자에서 치주건강 유지에 대한 전동치솔의 효과

박성준,이기헌,황현식 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.4

고정식 교정치료 환자들은 구강내 교정장치로 인해 치태제거에 어려움이 있으며 보다 효과적인 치태제거를 위해서는 특별한 구강위생교육 및 치솔질이 필요하다. 본 연구는 고정식 교정치료 환자에서 일반적으로 사용되고 있는 교정용 수동치솔에 비해 전동치솔이 치주건강 유지에 도움이 되는지 알아보기 위하여 시행되었다. 고정식 교정장치에 의해교정치료 예정인 환자 40명을 대상으로 고정식 교정장치 부착 1개월 후 치솔의 종류에 따라 전동치솔군과 수동치솔군으로 연구대상을 임의 구분하고 각각에 맞는 구강위생교육을 시행한 후 3개월, 6개월, 9개월, 12개월 후 치태지수, 치은염지수, 치은출혈지수, 치주낭깊이, 그리고 상대적부착상실을 측정하였다. 전동치솔의 경우 교정용 브러쉬 헤드를 가진 Braun Oral-B 사의 전동치솔을, 수동치솔의 경우 Butler 사의 교정용 치솔을 사용하게 하였다. 수동치솔군의 경우 실험기간 동안 치태지수는 통계적으로 유의하게 감소한 반면 (p<0.001), 치은염지수, 치은출혈지수는 통계적 유의차를 보이지 않았고, 치주낭깊이, 부착상실은 통계적으로 유의하게 증가하였다 (p<0.01). 전동치솔군의 경우에는 치은출혈지수와 함께 치주낭깊이, 부착상실이 통계적 유의차를 보이지 않았으며, 치태지수, 치은염지수는 통계적으로 유의하게 감소하였다 (p<0.001). 한편 치태지수, 치은염지수, 치은출혈지수의 경우 변화양상에 있어 전동치솔군과 수동치솔군 간에 유의한 차이를 보이지 않은 반면, 치주낭깊이와 부착상실의 경우 전동치솔군에서는 처음 상태가 일정하게 유지된 반면, 수동치솔군에서는 치주낭깊이와 부착상실이 증가하는 양상을 나타내었으며 이러한 변화양상의 수동치솔과 전동치솔간 차이는 통계적으로 유의하였다 (p<0.05). 이상의 결과는 고정식 교정장치로 치료받는 환자에서 수동치솔보다 전동치솔이 치주건강 유지에 더 효과적임을 시사하였다. The purpose of this study was to evaluate the efficacy of an electric toothbrush, with a specially designed orthodontic brush head, compared to a manual toothbrush on the periodontal health of patients with fixed orthodontic appliances. Forty patients were randomly divided into two groups, the electric and the manual toothbrush groups, 1 month after attachment of fixed orthodontic appliances. Periodontal status was measured using a plaque indes, a gingival index, a bleeding index, a pocket depth and a relative attachment loss, at baseline and after 3 months, 6 months, 9 months, and 12 months. The Braun Oral-B Plak Control with Ortho OD 15-1 brush head was used as the electric toothbrush while the Butler G.U.M 124 was given as the manual toothbrush. In the manual toothbrush group, the gingival and bleeding indices showed no statistlcally significant differences, but the plaque index was significantly decreased (p < 0.001) and pocket depth as well as relative attachment loss were significantly increased (p < 0.01). In the electric toothbrush group, the bleeding index, pocket depth and relative attachment loss showed no statistically significant differences, but the plaque and gingival indices were significantly decreased (p < 0.001).In the case of the plaque, gingival and bleeding indices, there were no statistically significant differences between the electric and the manual toothbrush groups. On the contrary, in the case of pocket depth and relative attachment loss, there were statistically significant differences between the electric and the manual toothbrush groups: an increase in the manual toothbrush groups unlike the electric toothbrush group which kept the same state (p < 0.05). These findings suggest that an electric toothbrush is useful for orthodontic patients with fixed appliances.