Nrf2-Heme oxygenase-1 modulates autophagy and inhibits apoptosis triggered by elevated glucose levels in renal tubule cells

Joo Heon Kim,김경민,Jin Uk Jeong,Jong-Ho Shin,신재민,방기태 대한신장학회 2019 Kidney Research and Clinical Practice Vol.38 No.3

Background: Autophagy is a highly balanced process in which lysosomes remove aged and damaged organelles and cellular proteins. Autophagy is essential to maintain homeostasis in the kidneys. Methods: Using human renal tubule cells HK-2, we assessed the impact of high glucose (HG) on autophagy. We also evaluated the capability of sulforaphane (SFN) to protect the HK-2 cells from HG-induced apoptosis by modulating autophagy. Results: SFN modulated autophagy and decreased apoptosis in the HK-2 cells that were cultured in 250 mM glucose medium for two days. The reactive oxygen species (ROS) levels increased, as expected, in the cells cultured in the 250 mM glucose medium. However, the SFN decreased the ROS levels in the HK-2 cells. The overexpression of heme oxygenase-1 (HO-1) by SFN decreased the expression of LC3 and beclin-1. LC3 and beclin-1 were involved in the downregulation of caspase-3 that was observed in the HG-induced cells. Conclusion: The activation of nuclear factor E2-related factor 2 (Nrf2)-HO-1 inhibited ROS expression and subsequently attenuated autophagy and cell apoptosis after HG injury was decreased. HG injury led to the activation of autophagy and HO-1 in order to combat oxidative stress and protect against cell apoptosis. Therefore, HO-1 activation can prevent ROS development and oxidative stress during HG injury, which considerably decreases autophagy and apoptosis.

Omeprazole 투여시 위산분비 억제능에 대한 CYP2C19 의 영향

박수헌(Soo Heon Park),김종원(Jong Won Kim),이수연(Soo Youn Lee),최명규(Myung Gyu Choi),김재광(Jae Kwang Kim),정인식(In Sik Chung),정규원(Gyu Won Chung),선희식(Hee Sik Sun),박두호(Doo Ho Park) 대한소화기학회 2001 대한소화기학회지 Vol.38 No.6

Background/Aims: CYP2C19 has an important role in the catabolism of several proton pump inhibitors. However, the relative contribution of CYP2C19-mediated metabolism varies among the different proton pump inhibitors. We investigate to determine the effect of CYP2C19 genotype status on intragastric pH during dosing with omeprazole. Methods: The subjects were 16 healthy volunteers. Their CYP2C19 genotype status was determined by a polymerase chain reaction-restriction fragment length polymorphism method. Twenty-four-hour monitoring of intragastric acidity were performed on the day without medication and day 4 after omeprazole administration. Results: A single dose of omeprazole significantly decreased 24 hour intragastric acidity (1.7±0.3 vs. 5.1±0.4). Subjects were divided into three groups on the basis of their CYP2C19 genotype status: homozygous extensive metabolizers (Ho-EMs, n=5), heterozygous extensive metabolizers (Ht-EMs, n=6), and poor metabolizers (PMs, n=5). Median 24 hour intragastric pH in the Ho-EM group was 3.1 compared with 5.5 in Ht-EM group and 5.9 in PM group(P<0.05). The median pH during omeprazole administration was influenced by CYP2C19 genotype. On the other hand, the Helicobacter pylori infection did not influence the median intragastric pH during omeprazole administration (p= NS). Conclusions: The effects of omeprazole on intragastric acidity is influenced by the CYP2C19 polymorphism. (Korean J Gastroenterol 2001;38:399-404)

冠岳樹木園地域內 荒廢山地土壤의 肥沃化를 통한 綠化促進에 關한 硏究

李宗學,麻鎬燮,權台鎬,李憲浩,禹保命 서울大學校農業生命科學大學附屬樹木園 1984 서울大學校 樹木園 硏究報告 Vol.- No.5

冠岳樹木園地域의 山腹斜面은 地質的으로 대보화강암에서 풍화생성된 거친 돌부수러기가 많은 粗砂質壤土로 구성되었으므로 表土層의 土壤流失이 심하여 土深이 대단히 얕거나 岩盤露出이 심하여 林木生長이 심히 不良한 地帶로서,山地의 地力恢復 및 現存하는 散生樹木의 生長力恢復을 通한 山地綠化保全이 시급히 요청되고 있다. 이와같은 요청에 부응하여 荒廢地斜面에 自生하고있는 주요한 4樹種(소나무, 리기다소나무, 노간주나무, 철쭉)에 대하여 山地施肥效果 試驗을 春期와 秋期로 나누어 실시한 결과 樹高 및 直徑生長量에 있어서 無施肥區에 比하여 현저한 施肥效果가 인정되었다. 따라서 冠岳山地帶의 荒廢地를 조속히 復舊綠化하기 위해서는 砂防土木的 工事方法 및 砂防造林的 播植方法이외에도 현존 수목의 保全 및 生長力恢復을 통한 速成綠化方法도 채용되어야 할 것이다. Hillsides in the Kwanak arboretum area were composed mainly of coarse sandy loam having relatively high proportion of rough weathered debris derived from the Daebo granite geologically. Owing to heavy soil erosion resulted to shallow soil depth and heavy rock exposures, the growing trees in this type of surface soil are very poor in their growth. For woody plant vegetation establishment in this montain area, it is urgent to recover the fertility of soil and the growth of existing trees. For this purpose, in spring and autumn season in 1983∼84 the fertilization experiment was conducted with the 4 principal native species(Pinus densiflora, Pinus rigida, Juniperus rigida, Rhododendron schlippenbachii) growing in this denuded forest land, and the effects of fertilized tree plots were more prominent than the control plot in both height and root-collar diameter growth. Therefore, the accelerating measures of vegetation rehabilitation through conservation of existing trees and recovery of tree growth by forest fertilization practices, besides forest engineering measur es and soil erosion control afforestation measures, could be acceptable as the measures for the recovery of denuded forest land in Mt. Kwanak area.

冠岳樹木園地域內 荒廢山地土壤의 肥沃化를 통한 綠化促進에 關한 硏究

李宗學,麻鎬燮,李憲浩,權台鎬,禹保命 서울大學校 農科大學 1984 서울대농학연구지 Vol.9 No.2

冠岳樹木園地域의 山復斜面은 地質的으로 대보화강암에서 풍화생성된 거친 돌부수러기가 많은 粗砂質壤土로 구성되었으므로 表土層의 土壤流矢이 심하여 土深이 대단히 얕거나 岩盤露出이 심하여 林木生長이 심히 不良한 地帶로서, 山地의 地力恢復 및 現存하는 散生樹木의 生長力恢復을 通한 山地綠化保全이 시급히 요청되고 있다. 이와같은 요청에 부응하여 荒廢地斜面에 自生하고 있는 주요한 4樹種(소나무, 리기다소나무, 노간주나무, 철쭉)에 대하여 山地施肥效果 試驗을 春期와 秋期로 나누어 실시한 결과 樹高 및 直徑生長量에 있어서 無施肥區에 比하여 현저한 施肥效果가 인정되었다. 따라서 冠岳山地帶의 荒廢地를 조속히 復舊綠化하기 위해서는 砂防土木的 工事方法 및 砂方造林的 播植方法이외에도 현존 수목의 保全 및 生長力恢復을 통한 速成綠化方法도 채용되어야 할 것이다. Hillsides in the Kwanak arboretum area were composed mainly of coarse sandy loam having relatively high proportion of rough weathered debris derived from the Daebo granite geologically. Owing to heavy soil erosion resulted to shallow soil depth and heavy rock exposures, the growing trees in this type of surface soil are very poor in their growth. For woody plant vegetation establishment in this montain area, it is urgent to recover the fertility of soil and the growth of existing trees. For this purpose, in spring and autumn season in 1983~84 the fertilization experiment was conducted with the 4 principal native species(Pinus densifltora, Pinus rigida, Juniperus rigida, Rhododendron schlippenbachii) growing in this denuded forest land, and the effects of fertilized tree plots were more prominent than the control plot in both height and root-collar diameter growth. Therefore, the accelerating measures of vegetation rehabilitation through conservation of existing trees and recovery of tree growth by forest fertilization practices, besides forest engineering measur es and soil erosion control afforestation measures, could be acceptable as the measures for the recovery of denuded forest land in Mt. Kwanak area.

A Case of Urethral Stent for Recurrent Urethral Stricture and Penile Prosthesis for Erectile Dysfunction

Jung, Gyung-Woo,Song, Young-Jin,Park, Young-Ho,Kwon, Heon-Young,Yoon, Jin-Han 동아대학교의과대학 1992 동아의대학술지 Vol.4 No.1

독성 물질을 함유한 산업폐수의 생물학적 처리에 관한 연구

최용호,이헌모 동의공업대학 2000 論文集 Vol.26 No.1

Chemical industry wastewaters are highly variable in composition, depending on plant production schedules, and composed principally of soluble organic substances which may not be removed by precipitation and adsorption, and may contain toxic chemical species which would interfere with biological treatment processes. This study was conducted to evaluate the biological treatability of the complex organic chemical wastewater by a complete mixing activated sludge process and the toxic effects on the biological activities through BOD tests at different dilution ratios. Even though the BOD/CODcr and BOD/COD Mn ratio of the composited wastewater appeared to be 0.7 and 8.3, respectively, at least 20 times dilution of the wastewater was required for the biological treatment due to the toxic effects of chemicals in the wastewater on biological activities. BOD data at different dilution ratio could be used to evaluate the optimum dilution for biological treatment of toxic wastewater.

1920年代 前半期 『每日申報』의 反一社會主義 談論 硏究

박헌호 동국대학교 한국문학연구소 2005 한국문학연구 Vol.0 No.29

사회주의는 식민지 조선에서 가장 강력한 탄압을 받았던 사상이다. 일제는 다양한 정책과 폭력을 동원하여 사회주의를 탄압하는 한편, 사회주의에 대한 부정적·비판적 인식을 대중에게 심어주기 위해 반-사회주의 선전을 지속적으로 실행했다.『매일신보』는 그 중심적인 매체였다.일제는 『매일신보』 지면을 통해 러시아가 사회주의 혁명으로 말미암아 굶주림과 무질서의 국가로 전락했음을 강조하는가 하면, 사회주의를 성적 방탕의 대명사로 인식시키는 선전 전략을 구사했다. 무질서와 성적 방탕은 이후 사회주의를 비판하는 핵심 개념이 됐다. 일제는 사회주의 의 철학적 기반인 '유물론'을 정신의 존재를 부정하는, 혹은 정신적 가치를 무시하는 물질지상주의적 철학으로 왜곡시키기도 하였다. 이시기, 『매일신보』에 나타난 반-사회주의 담론은 이후 지속되는 일제의 반-사회주의 선전의 핵심을 선취하고 있다. 일제의 반-사회주의 선전은 조선의 지식인들에게도 심각한 영향을 미쳐 한국 근대사상사를 굴절시키는 중대한 영향을 미친 것으로 판단된다. In the colony Joseon, socialism was the most forcefully suppressed thought. Japan Imperialism suppressed socialism with the various measures and violence, and also continued to propagate an anti-socialism in oder to inculcate th mass with the negative or critic idea on socialism. Japan Imperialism propagated in many articles of Maeilsinbo. Russia degraded into the country of starvation and disorder itself because of 1917' socialism revolution, and also expressed socialism as a thought fostering sexual dissipation. Disorder and sexual dissipation became the core concepts of criticizing socialism. Materialism, the philosophical base of socialism, was condemned as a thought subliming materiality of neglecting spiritual values or existence of sprit. These anti-socialism discourse of Maeilsinbo took first main contents of anti propaganda on this thought continued by Japan Imperialism from this time. This has an great influence on the colonial intellectuals, which I think refracted history of Korean modern thoughts in the result.

GC/MS-SIM과 ³²P-postlabeling방법에 의한 염료와안료를 제조하는 사업장 근로자들의 방광암 조기발견을 위한 Biomarker개발에 관한 연구

이진선,신호상,김양호,노재훈,이범규 공주대학교 자원재활용신소재지역협력센터 2000 2차년도 센터 사업 성과집 Vol.2000 No.1

To develop the biomarker of non-invasive biomonitoring among workers who exposed benzidine-based dyes in its manufacturing and treating industries, we examined and evaluated the specific carciaogen-DNA adducts in exfoliated urothelial cells of 33 exposed workers. Samples were collected from urine of workers, and then isolated DNA from them. DNA adducts were analyzed using 32P-postlabeling, along with Nuclease P1. One adducts was predominated in those samples with 6 adducts present. It was formed in all exposed workers, but the others not. Its relative adducts level(RAL) was 73.2×107, which was statistically significantly higher than any other adducts' level(p<0.05). Analyzing regression between its RAL and employment period of workers, simple linear regression was positively increased with slop of 6.922(p<0.05, r2=0.548). And also, DNA adducts were analyzed using GC/MS-SIM, along with n-butanol extraction and trifluoroacetyl auhydride(TFA) derivatization. Standard material was synthetic N-(deoxyguanosin-8-yl)-N'-acetylbenzidine. The characteristics of sample and standard were observed on the same sites, such as M+(m/z 376) and M+(m/z 279) for benzidine-di-TFA and mono-TFA. And then, they were confirmed by analyzing with secondary ion mass(SIM). This study showed that benzidine-based dyes was formed one DNA adduct into significantly high levels in bladder of all exposed workers, its chemical formula was N-(deoxyguanosin-8-yl)-N'-acetylbenzidine. So we can do monitoring the workers with exfoliated urothelial cells of them by analyzing above DNA adduct as biomarker for preventing bladder cancer causing the exposure of the benzidine-based dyes.

접착제의 다층적용이 복합레진의 전단결합강도에 미치는 영향

박헌동,이난영,이창섭,이상호 대한소아치과학회 2006 大韓小兒齒科學會誌 Vol.33 No.3

간세포암에 대한 간동맥 화학색전술의 치료효과

이헌영,김병호,정현용,김영건,조준식 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

Hepatocellular carcinoma(HCC) is the second most common cause of cancer death in Korea. Generally, HCC was unresectable due to the frequently associated cirrhosis and advanced stage of tumor. As a palliative therapy of HCC, transcatheter arterial chemoembolization(TACE) was a proven effective mean of treating inoperable HCC. We have analyzed the effectiveness of TACE in inoperable HCC. TACE was performed in 20 patients from January in 1990 to May in 1993 at Chung Nam National University Hospital with the infusion of mixture of Lipiodol and Adriamycin. We analyzed the survival rates of the TACE group and the control group who received no specific treatment. The results were follows ; 1) The survival rate was significantly higher in TACE group than control group(p<0.01). The survival rate at the end sixth months and one year were 62.8% and 40.0% in TACE group, 26.6% and 0% in control group, respectively. 2) A declining level of AFP(Alph-fetoprotein) was shown in 8 among 10 cases who underwent TACE/and was higher than 500 ng/ml in pre-TACE level(p<0.01) and also tumor size was decreased in 7 among 20 cases who underwent TACE. 3) Side effects were fever(80.0%), and upper abdominal pain(75.0%) in TACE group but improved within 7 days after TACE and impairment of liver function was developed in most patients(TACE group) but improved within 2 weeks after TACE. As a palliative therapy of HCC, TACE is an effective and useful modality in treating and prolonging the survival time of patients with unresectable hepatocellular carcinoma.