Master Chongsan`s proposal for "Universal Harmony" : In view of the Zen Buddhist Philosohy of ishitani Keji

Heine, Steven 한국원불교학회 2001 圓佛敎學 Vol.7 No.-

Quantitation and Validation of Atorvastatin using HPLC-UV

Heine, Daniel,Yong, Chul-Soon,Kim, Jung-Sun The Korean Society of Pharmaceutical Sciences and 2007 Journal of Pharmaceutical Investigation Vol.37 No.3

A reversed phase HPLC analysis of atorvastatin (AS) standard solution was performed using diclofenac (DF) as internal standard. Column oven temperature, flow rate and the composition of the mobile phase were varied in order to determine a practical system setup using a C18 column and UV detector. Two C18 columns of different length were compared regarding their influence on the AS peak shape. Based on these preliminary experiments a validation study was performed utilizing a C18 column at $62^{\circ}C$ with a mobile phase consisting of sodium phosphate buffer (0.05 M, pH 4.0), methanol and acetonitrile (40:50:10, v/v/v). The detection limit for AS was $0.1{\mu}g/ml$ and inter- and intra-day calibration curves were linear over a concentration range of $0.2-50{\mu}g/ml$. Accuracy and precision were satisfactory in the AS concentration range of $0.5-50{\mu}g/ml$.

Bioinformatics Analysis of Hsp20 Sequences in Proteobacteria

Heine, Michelle,Chandra, Sathees B.C. Korea Genome Organization 2009 Genomics & informatics Vol.7 No.1

Heat shock proteins are a class of molecular chaperones that can be found in nearly all organisms from Bacteria, Archaea and Eukarya domains. Heat shock proteins experience increased transcription during periods of heat induced osmotic stress and are involved in protein disaggregation and refolding as part of a cell's danger signaling cascade. Heat shock protein, Hsp20 is a small molecular chaperone that is approximately 20kDa in weight and is hypothesized to prevent aggregation and denaturation. Hsp20 can be found in several strains of Proteobacteria, which comprises the largest phyla of the Bacteria domain and also contains several medically significant bacterial strains. Genomic analyses were performed to determine a common evolutionary pattern among Hsp20 sequences in Proteobacteria. It was found that Hsp20 shared a common ancestor within and among the five subclasses of Proteobacteria. This is readily apparent from the amount of sequence similarities within and between Hsp20 protein sequences as well as phylogenetic analysis of sequences from proteobacterial and non-proteobacterial species.

The European System of Scaling Strength of Play

Klaus Heine 국제바둑학회(구 한국바둑학회) 2003 국제학술대회논문집 Vol.2003 No.1

The Chile-China Paradox: Burgeoning Trade, Little Investment

( Jorge Heine ) 경남대학교 극동문제연구소 2016 ASIAN PERSPECTIVE Vol.40 No.4

Chile, a country that has been at the forefront of Latin America`s growing links with China, embodies a paradox in terms of Chinese outward foreign direct investment (COFDI). On the one hand, bilateral trade has thrived, increasing fourfold since the signing of a free trade agreement in 2005, turning Chile into China`s third largest trading partner in the region. On the other hand, Chinese direct investment in Chile has been low, much lower than in neighboring countries. In this article I explore the roots of this upsurge in bilateral trade while also explaining the reasons for the dearth of Chinese investment in a country traditionally considered to be attractive to foreign investors. Chile might be a victim of its own success as its economic accomplishments allow it to persist with investment policies that are currently daunting to Chinese investors.

The Linkage between Reverse Gyrase and Hyperthermophiles: A Review of Their Invariable Association

Michelle Heine,Sathees B.C. Chandra 한국미생물학회 2009 The journal of microbiology Vol.47 No.3

With the discovery of reverse gyrase in 1972, from Yellowstone National Park, isolated from Sulfolobus acidocaldarius, it has been speculated as to why reverse gyrase can be found in all hyperthermophiles and just what exactly its role is in hyperthermophilic organisms. Hyperthermophiles have been defined as organisms with an optimal growth temperature of above 85°C. Reverse gyrase is responsible for the introduction of positive supercoils into closed circular DNA. This review of reverse gyrase in hyperthermophilic microorganisms summarizes the last two decades of research performed on hyperthermophiles and reverse gyrase in an effort to provide an up to date synopsis of their invariable association. From the data gathered for this review it is reasonable to hypothesize that reverse gyrase is closely tied to hyperthermophilic life.

Master Chongsan`s proposal for “Universal Harmony”

Steven Heine 한국원불교학회 2001 圓佛敎學 Vol.7 No.-

The Personality of Luxury Fashion Brands

Klaus Heine 한국마케팅과학회 2010 Journal of Global Fashion Marketing Vol.1 No.3

The focus of brand differentiation is shifting increasingly to symbolic benefits mainly because of changing market conditions and consumer preferences. On the one hand, the functional benefits of many products on the market today become increasingly equivalent and exchangeable. On the other hand, an increasing number of consumers engage in symbolic consumption and decide for a product mainly due to the congruity between their personality and the symbolic personality of the product or brand. These trends lead to an increased interest in the brand personality concept, which offers a systematic approach to create symbolic benefits. Although luxury brands are characterised with strong symbolic benefits that often even exceed their functional benefits and that refer to a large extent to human personality traits, there exists only a small literature base about the symbolic meaning of luxury brands and no specific brand personality framework. This paper sets a foundation for a luxury brand personality framework with an investigation of personality traits as the basic elements of a brand personality. More specifically, its objective is to uncover the entire universe of personality traits that luxury brands represent in the eyes of their consumers based on two empirical studies. These studies focus on luxury fashion brands as this industry covers the biggest variety of brand images. As a prerequisite, this paper defines luxury brands as the objects of investigation, explains the concept of brand personality and its common research methodology and outlines the requirements and selection criteria for luxury brand personality traits. The brand personality refers to the set of human characteristics associated with a brand. Aaker developed the most established theoretical framework of brand personality dimensions and a scale to measure them by drawing on research about the Big Five human personality dimensions. More than 600 U.S. respondents rated on a five-point Likert scale a subset of 37 general brands of varying categories on 114 personality traits. Aaker consolidated the personality traits by factor analyses to five distinct dimensions. This paper proposes a research methodology specifically for the investigation of luxury brand personality traits. It builds on a consumer-oriented qualitative approach using the repertory grid method (RGM), which is constrained by a conceptual framework of guidelines and selection criteria, but remains flexible enough to consider the ambiguous and contextual aspects of brand personality. While the quantitative approach requires deleting ambiguous and contextual traits that load on multiple factors, RGM allows respondents to describe constructs with a group of words and enables researchers to decode their varying contextual meanings for different constructs. In addition, RGM matches the consumer-orientation in brand management as the resulting sets of traits and brands originate directly from the respondents. A major modification to the common research approach is that each trait has to consist of three adjectives. While a single adjective can be very ambiguous, word combinations become more precise as people can rely on their overlapping meaning. The first study covers in-depth interviews with about 50 luxury consumers about their associations with luxury brands according to the RGM and led to a set of 49 personality traits and five major personality dimensions. These dimensions include modernity, eccentricity, opulence, elitism, and strength. Modernity describes the temporal perspective of a brand, which can lie either in the past or in the present or future. Eccentricity describes the level of discrepancy from social norms and expectations. Opulence refers to the level of conspicuousness of the symbols of wealth. These symbols cover a wide range of associations including ostentatious logos and valuable materials.

Luxury goes digital: how to tackle the digital luxury brand–consumer touchpoints

Klaus Heine,Benjamin Berghaus 한국마케팅과학회 2014 Journal of Global Fashion Marketing Vol.5 No.3

Today, many of the big brands are diverting substantial amounts of resources away from classical communication channels toward the digital domain. However, despite the digital gold rush, major sections of luxury brands still have not fully arrived in the digital world. Their sentiment increasingly shifts from “Do we need to be online?” to a more complicated question: “How do we actually do it right?” Therefore, this paper has two major objectives. First of all, we want to provide luxury marketers with an overview of the major digital luxury brand–consumer touchpoints and corresponding digital working areas. The first step to digital excellence is to know the available digital opportunities. Based on that, we want to derive some recommendations regarding how to tackle the digital channels successfully, based on an expert survey and a discussion of case studies for each major digital touchpoint. The paper concludes with some major lessons learned.

Bioinformatics Analysis of Hsp20 Sequences in Proteobacteria

Michelle Heine,Sathees B.C.Chandra 한국유전체학회 2009 Genomics & informatics Vol.7 No.1

Heat shock proteins are a class of molecular chaperones that can be found in nearly all organisms from Bacteria, Archaea and Eukarya domains. Heat shock proteins experience increased transcription during periods of heat induced osmotic stress and are involved in protein disaggregation and refolding as part of a cell’s danger signaling cascade. Heat shock protein, Hsp20 is a small molecular chaperone that is approximately 20kDa in weight and is hypothesized to prevent aggregation and denaturation. Hsp20 can be found in several strains of Proteobacteria, which comprises the largest phyla of the Bacteria domain and also contains several medically significant bacterial strains. Genomic analyses were performed to determine a common evolutionary pattern among Hsp20 sequences in Proteobacteria. It was found that Hsp20 shared a common ancestor within and among the five subclasses of Proteobacteria. This is readily apparent from the amount of sequence similarities within and between Hsp20 protein sequences as well as phylogenetic analysis of sequences from proteobacterial and non-proteobacterial species. Heat shock proteins are a class of molecular chaperones that can be found in nearly all organisms from Bacteria, Archaea and Eukarya domains. Heat shock proteins experience increased transcription during periods of heat induced osmotic stress and are involved in protein disaggregation and refolding as part of a cell’s danger signaling cascade. Heat shock protein, Hsp20 is a small molecular chaperone that is approximately 20kDa in weight and is hypothesized to prevent aggregation and denaturation. Hsp20 can be found in several strains of Proteobacteria, which comprises the largest phyla of the Bacteria domain and also contains several medically significant bacterial strains. Genomic analyses were performed to determine a common evolutionary pattern among Hsp20 sequences in Proteobacteria. It was found that Hsp20 shared a common ancestor within and among the five subclasses of Proteobacteria. This is readily apparent from the amount of sequence similarities within and between Hsp20 protein sequences as well as phylogenetic analysis of sequences from proteobacterial and non-proteobacterial species.