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Heeyeon BAEK,Joo Young PARK,Yong Hwan KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Lignin peroxidase is the promising enzyme spanning various industries such as cosmetics and biorefinery because of its high redox potential which can oxidize phenolic and even non-phenolic compounds. Although its high potential to industrial application, it is still challenging because of its complexity in the production, especially expression and purification. This complexity is originated from four disulfide bonds of lignin peroxidase which expressed as an insoluble form in Escherichia coli requiring unfolding and refolding in vitro. Nevertheless, removing disulfide bond by rational design has not been attempted because it is known that disulfide bond contributes to stabilize the tertiary structure of proteins. But in our previous study, we obtained the triple mutant of PcLiP01 which have incredibly high thermodynamic stability by introducing the interaction between heme and neighboring residues. In this study, we designed mutants to remove the disulfide bond of the triple mutant with site-directed mutagenesis for soluble expression of recombinant lignin peroxidase in E. coli.
Heeyeon Kim(김희연),Woongshik Nam(남웅식),Seong Hee Kim(김성희),Hye Ryang Jang(장혜량),Mi Kyoung Lee(이미경),Tae Wan Kim(김태완),Sangkyu Lee(이상규) 한국생명과학회 2012 생명과학회지 Vol.22 No.8
인삼 및 인삼 유래 물질들은 가장 대표적인 건강기능식품이며, 홍삼추출물(Panax ginseng C.A. Meyer)의 경우 항바이러스, 알레르기 감소, 항산화, 비만억제 등의 효과를 나타낸다고 알려져 있다. 대표적 건강기능식품임에도 불구하고 약물상호작용을 일으키는 대사효소(Cytochrome P450, CYP)에 대한 홍삼추출물의 영향은 보고되지 않았다. 본 연구에서는 사람의 간 마이크로좀 분획과 마우스 모델을 기반으로 홍삼엑기스의 약물대사 조절능을 평가하였다. 사람의 간 마이크로좀에서는 CYP2B6에 대한 미약한 억제 효과를 나타내었을 뿐, 타 대사효소 CYP1A2, 2B6, 2C19, 2D6와 3A에 대한 억제효과는 검출되지 않았다. 홍삼엑기스를 마우스에 50, 250, or 500m g/kg의 농도로 3, 7, 14일간 하루 1회 경구 투여 후 간을 적출하여 대사효소의 활성 변화를 측정하였지만, 유의적인 변화는 관찰되지 않았다. 결과적으로 홍삼엑기스의 유의적인 약물대사효소에 대한 활성 조절능이 없는 것으로 판단되고, 홍삼엑기스 복용에 따른 홍삼-약물상호작용의 가능성은 없을 것으로 추정된다. Ginseng is one of the most commonly used herbal medicines and health foods. Korean red ginseng (KRG; Panax ginseng C.A. Meyer) extract is known to have potential therapeutic activities, such as anti-viral effects, the amelioration of food allergies, anti-oxidant effects, and obesity reduction. Nevertheless, no reports have been issued the modulatory effects of KRG extract on the activity of cytochrome P450 (CYP). In the present study, we investigated the modulatory effect of KRG extract in vitro and in vivo by using pooled human liver microsomes and male ICR mice. When human liver microsomes were incubated with KRG extract at 0.01-10 mg/ml, CYP1A2, 2B6, 2C19, 2D6, and 3A were not significantly inhibited by KRG extract, although CYP2B6 was slightly inhibited. Mice were orally administered KRG extract at 50, 250, or 500 mg/kg daily for 3, 7, or 14 days. However, the activities of CYPs in mouse livers were not significantly different from those of vehicle-treated controls. In conclusion, no significant ginseng-drug interaction was observed. KRG extract did not significantly modulate the activities of CYPs in vitro or in vivo.