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병재배 폐톱밥을 이용한 양송이 복토재료 개발에 관한 연구
김홍규,이희덕,김용균,한규홍,문창식,김홍기 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-
애느타리 병재배후 탈병한 톱밥을 3월 중순 퇴적하여 부숙시킨 결과 퇴적기간에 따른 폐톱밥의 부숙정도는 퇴적 30일이후에 가장 컸으며 부숙 소요일수는 최소 48일이었다. 퇴적 기간에 따른 폐톱밥의 pH 및 총 질소 함량은 부숙이 진전됨에 따라 증가하는 경향이었고 총 탄소 및 C/N율은 시간이 경과함에 따라 감소하는 경향이었다. 식양토에 부숙이 완료된 톱밥을 부피 비율로 10, 30, 50%씩 첨가하여 복토 재료로 사용한 결과 30% 첨가시 식양토 단용 처리보다 A. bisporus 균사 생장이 촉진되고 초발이 소요일수가 5일 빠르며, 수량에 있어서도 28% 증수되었다. After bottle culture of Pleurotus ostreatus, sawdust was taken out from the bottle and accumulated in the middle of March, and then composted. As the result, Y value was decreased rapidly 30 days after composting, and it was decreased slowly after 30 days. It is considered that 118 days is required for composting, however, it is possible to use for casing material after at least 48 days composting. The pH and total nitrogen content of sawdust based on composting period had tendency to increase as composting was processed. Total carbon and C/N rate had tendency to decrease as time went on. Based on the rate of 10, 30 and 50%, each sawdust was added to clay loam used as casing material for culturing A. bisporrus. Among various treatments, the mycelial growth of A. bisporus was more favorable in the treatment of 30% sawdust than in the single treatment of clay loam. Based on the date necessary for primodium formation of A. bisporus, the primodium formation in the treatment of 30% sawdust was reduced to about 5 days as compared with that of any other treatments When 30% sawdust was added to clay loam used as casing material for culturing A. bisporus, the yield of its fruiting body was increased to 28%.
Development of Neutaribeosut Varieties (Pleurotus ostreatus) Chongpung, Myongwol
He-duck Lee,Hong-kyu Kim,Young-gyun Kim,Kyu-heng Han,Kwang-ho Min,Tae-wo Jeong,Jae-jung Lee,Tae-sun Jeon,Duck-hwan Kim,Hun-sick Kim,Jong-ku Han 한국자원식물학회 2000 Plant Resources Vol.3 No.2
This study was carried out to find a useful mushroom at Chungnam Agricultural Research And Extention Service. Twenty materials used were collected from domestic and exotic area. These races were compared bontanical characteristics to leading varieties by PCR-RAPD methods. Mycelial growth temperature of Chongpung and Myongwol were at 20 to 250 and 25 to 30°C at PDA medium, respectively mycelial growth of these varieties were similiar at pH 6.5 to 7,5. In case of mushroom cultivation temperature ranges, Chongpung was at 5 to 26 and Myongwol was at 7 to 28C, but the optimum temperature range of these were appeared at 15 to 190. Culture temperature of these was 23°C and period of mycelial culture was needed 23 to 24 days under 850 cc/pp, while was needed 11 to 12 days at waste cotton medium. Cap color of these at first inducing mushroom was all dark blue, but at late growing stages Chongpung was shown as grey, and Myongwol was shown as dark grey. Yield of Chongpung was appeared as 46kg/3.3m and that of Myongwol was 41 kg /3.3m , while Chunchu No2 as check was 40 kg/3.3m. Results from PDA medium and PCR RAPD analysis two of these were different from others.
이희덕(He-duck Lee),김용균(Yong-gyun Kim),최현구(Hyun-gu Choi) 한국자원식물학회 2003 한국자원식물학회지 Vol.16 No.3
느타리버섯 재배시 기존의 고압살균처리는 영세한 버섯재배 농민의 시설비나 운영면에서 비용이 많이 들어 야외 발효나 저온살균에 의한 저비용 고효율의 최적배지 선발을 위한 살균방법별 시험결과를 요약하면 가. 배지 재료에서는 톱밥+면실+왕겨 (1:1:1)배지와 톱밥+면실+비트 (5:3:2)처리가 비슷한 경향이었고 나. 살균방법 처리에서는 고압살균(121℃, 90분) 방법보다 저온살균(5060℃, 7일)처리가 균사배양 및 생육특성이 양호하였으며 다. 기존의 고압살균 톱밥+면실+비트(5:3:2)처리구 174g/1.5kg 대비 저온살균 톱밥+면실+왕겨 (1:1:1) 205g/1.5kg으로 18%증수되고 톱밥+면실+비트(5:3:2) 처리구에서는 207g/1.5kg으로 19% 증수되는 경향이었으나 톱밥+면실에 왕겨를 첨가하나 비트펄프를 첨가하나 유의성이 없었다. 라. 봉지 재배시 저온살균이 고압살균대비 경영비 12% 절감과 농가소득 향상에 기여 할 것으로 기대된다 This study was carried out to find optimum condition through sterilization methods for cultivation of Neutaribeosut (Oyster mushroom). 1. Yield of additive culture of pine sawdust, cotton and rice hulls at bagcultivation of Neutaribeosut. was similiar to additive culture of pine sawdust, cotton and pulpe as conventional methods. 2. Yield of Neutaribeosut at low temp. sterilization method as 50 to 60℃ and 7days treatment was higher about 18 to 19% than that of high temp. as 121℃ and 90 minutes treatment. 3. In case of farmercost, low than high temperature sterilization method appeared lowly about 12%. So this method will be of advantage to farmers in both yield and farmercost.