First Report of the Peanut Stripe Strain of Bean common mosaic virus (BCMVPSt)Infecting Mungbean in Korea

최홍수,박진우,김국형,이수헌,김정수,김미경,최장경,Hassan Karakacha Were,Yoichi Takanami 한국식물병리학회 2006 Plant Pathology Journal Vol.22 No.1

A virus causing chlorotic ringspot, yellow mosaic and vein clearing symptoms was prevalent on mungbean plants around Taean, Korea. The isolate caused mosaic on Chenopodium quinoa, Nicotiana benthamiana, Phaseolus vulgaris and Vicia faba but no symptoms on peanut plants. Inclusion bodies such as scroll, pinwheel and laminated aggregates induced by the virus in the host cells were similar to those produced by members of the Potyvirus subdivision III. Multiple alignment as well as cluster dendrograms of the 709 nucleotide region comprising part of the coat protein gene and 3'- untranslated region (UTR) showed that the isolate belongs to the BCMV-PSt subgroup. Altogether, these results support the identification of the causal virus as peanut stripe strain of Bean common mosaic virus (BCMV-PSt).

Occurrence of Bean common mosaic virus (BCMV) Infecting Peanut in Korea

최홍수,전정욱,최장경,김미경,박진우,김국형,김정수,Hassan Karakacha Were,Yoichi Takanami 한국식물병리학회 2006 Plant Pathology Journal Vol.22 No.1

A virus causing vein banding, sometimes yellow mosaic and rugose symptoms on peanut was prevalent around Suwon, Korea. A survey conducted in the area found disease incidence, depending on cultivar, to range from 79 to 100%. The virus was found to be seed-transmissible in all the five peanut cultivars tested with transmission rates ranging from 2 to 16%. Host range analysis failed to differentiate 9 field isolates collected from different peanuts cultivars showing various symptoms. Inclusion bodies such as scroll, pinwheel and long laminated aggregates induced by the virus in host plant cells were similar to those induced by members of the Potyvirus subdivision III. The virus showed < 95% homology with Bean common mosaic virus (BCMV), BCMV-BICMV/AzMV strains and only < 91% with Desmodium mosaic virus. Based on biological characterization, electron microscopy and molecular analyses of a Korean isolate (Daewon 1), the virus was identified as peanut stripe strain of BCMV.

Characteristics of Potato virus Y Isolated from Paprika in Korea

최홍수,김미경,박진우,김국형,고숙주,최장경,Su-Heon Lee,Hassan Karakacha Were,Yoichi Takanami 한국식물병리학회 2005 Plant Pathology Journal Vol.21 No.4

A virus isolate collected from infected paprika (Capsicum annuum var. grossum) was characterized as Potato virus Y (PVY) based on biological, serological, cytopathological, and molecular properties. In host range studies, the paprika isolate produced the mosaic symptom on some tobacco, tomato and pepper (Capsicum annuum). A new paprika isolate also infected potato cultivars which is different biological characteristic compared to the other popular potyvirus infecting paprika, Pepper mottle virus (PepMoV). Previously reported PVY strains, PVYO and PVYN did not infect pepper and typical PepMoV isolates did not infect potato. Distinctive inclusion patterns of the scroll, pinwheel, long laminated inclusions, and helper components in the cytoplasm of infected cells were also different to those observed by the typical PVY isolate infections. However, the paprika isolate reacted to the monoclonal antibody of PVYN strain with high absorbance readings. RT-PCR amplification, cloning,and sequencing of the 3' untranslated region and a part of coat protein gene also added additional evidence of the paprika isolate as the PVYN-related isolate. Multiple alignments as well as cluster dendrograms of PVYpaprika isolate revealed close phylogenetic relationship to the PVYN subgroup. Altogether, these results suggest that a new PVY isolate infecting paprika contained distinct characteristics compared to the other previously described PVY strains with closer relationship to the PVYN strain.

Virulence Differentiation of Eight Turnip mosaic virus Isolates Infecting Cruciferous Crops

최홍수,윤무경,최장경,김국형,천정욱,Seong-Han Sohn,Jeong-Soo Kim,Hassan Karakacha Were,Yoichi Takanami 한국식물병리학회 2005 Plant Pathology Journal Vol.21 No.4

Turnip mosaic virus (TuMV) is an infectious viral pathogen on the cruciferous crops, predominantly Chinese cabbage (Brassica campestris subsp. pekinensis) and radish (Raphanus sativus). On the basis of the symptom development in selective differential hosts from indicator host species, Chinese cabbage and Korean radish inbred lines, the representative eight isolates of TuMV were divided into two major groups/or six types. Group I includes Tu 1, Ca-ad7, and Cj-ca2-1 isolates, while group II includes the other isolates (rg-pf1, r 9-10, Rhcq1- 2, Stock and Mustard). According to the molecular phylogenetic analysis, these isolates, however, divided into two groups and two independent isolates. Phylogenetic analysis indicated that four isolates (Tu 1, r 9-0,Stock and Rh-cq1-2) formed a distinct phylogenetic group, and the other two isolates (Ca-ad7 and Cj-ca2-1) also formed another group. Mustard and rg-pf1 isolates did not seem to have any relationship with these two groups. Taken together, these results indicated that virulence differentiation on host plants, molecular phylogenetic analysis of the nucleotide and the deduced amino acid of TuMV coat proteins did not show any relationship. The multi-resistant lines, Wonyae 20026 and BP058 in Chinese cabbage represent valuable genetic materials that can be used for crucifer breeding programs on TuMV resistance, but not in Korean radish.

First Report of the Peanut Stripe Strain of Bean common mosaic virus (BCMVPSt) Infecting Mungbean in Korea

Choi, Hong-Soo,Kim, Mi-Kyeong,Park, Jin-Woo,Lee, Su-Heon,Kim, Kook-Hyung,Kim, Jeong-Soo,Were, Hassan Karakacha,Choi, Jang-Kyung,Takanami, Yoichi The Korean Society of Plant Pathology 2006 Plant Pathology Journal Vol.22 No.1

A virus causing chlorotic ringspot, yellow mosaic and vein clearing symptoms was prevalent on mungbean plants around Taean, Korea. The isolate caused mosaic on Chenopodium quinoa, Nicotiana benthamiana, Phaseolus vulgaris and Vida laba but no symptoms on peanut plants. Inclusion bodies such as scroll, pinwheel and laminated aggregates induced by the virus in the host cells were similar to those produced by members of the Potyvirus subdivision III. Multiple alignment as well as cluster dendrograms of the 709 nucleotide region comprising part of the coat protein gene and 3'untranslated region (UTR) showed that the isolate belongs to the BCMV-PSt subgroup. Altogether, these results support the identification of the causal virus as peanut stripe strain of Bean common mosaic virus (BCMV-PSt).

Characteristics of Potato virus Y Isolated from Paprika in Korea

Choi, Hong-Soo,Ko, Sug-Ju,Kim, Mi-Kyeong,Park, Jin-Woo,Lee, Su-Heon,Kim, Kook-Hyung,Were, Hassan Karakacha,Chois, Jang-Kyung,Takanami, Yoichi The Korean Society of Plant Pathology 2005 Plant Pathology Journal Vol.21 No.4

A virus isolate collected from infected paprika (Capsicum annuum var. grossum) was characterized as Potato virus Y (PVY) based on biological, serological, cytopathological, and molecular properties. In host range studies, the paprika isolate produced the mosaic symptom on some tobacco, tomato and pepper (Capsicum annuum). A new paprika isolate also infected potato cultivars which is different biological characteristic compared to the other popular potyvirus infecting paprika, Pepper mottle virus (PepMoV). Previously reported PVY strains, $PVY^o$ and $PVY^N$ did not infect pepper and typical PepMoV isolates did not infect potato. Distinctive inclusion patterns of the scroll, pinwheel, long laminated inclusions, and helper components in the cytoplasm of infected cells were also different to those observed by the typical PVY isolate infections. However, the paprika isolate reacted to the monoclonal antibody of $PVY^N$ strain with high absorbance readings. RT-PCR amplification, cloning, and sequencing of the 3' untranslated region and a part of coat protein gene also added additional evidence of the paprika isolate as the $PVY^N$-related isolate. Multiple alignments as well as cluster dendrograms of PVY-paprika isolate revealed close phylogenetic relationship to the $PVY^N$ subgroup. Altogether, these results suggest that a new PVY isolate infecting paprika contained distinct characteristics compared to the other previously described PVY strains with closer relationship to the $PVY^N$ strain.

Virulence Differentiation of Eight Turnip mosaic virus Isolates Infecting Cruciferous Crops

Choi, Hong-Soo,Sohn, Seong-Han,Yoon, Moo-Kyoung,Cheon, Jeong-Uk,Kim, Jeong-Soo,Were, Hassan Karakacha,Cho, Jang-Kyung,Kim, Kook-Hyung,Takanami, Yoichi The Korean Society of Plant Pathology 2005 Plant Pathology Journal Vol.21 No.4

Turnip mosaic virus (TuMV) is an infectious viral pathogen on the cruciferous crops, predominantly Chinese cabbage (Brassica campestris subsp. pekinensis) and radish (Raphanus sativus). On the basis of the symptom development in selective differential hosts from indicator host species, Chinese cabbage and Korean radish inbred lines, the representative eight isolates of TuMV were divided into two major groups/or six types. Group I includes Th 1, Ca-ad7, and Cj-ca2-1 isolates, while group II includes the other isolates (rg-pfl, r 9-10, Rhcql-2, Stock and Mustard). According to the molecular phylogenetic analysis, these isolates, however, divided into two groups and two independent isolates. Phylogenetic analysis indicated that four isolates (Tu 1, r9-10, Stock and Rh-cql-2) formed a distinct phylogenetic group, and the other two isolates (Ca-ad7 and Cj-ca2-1) also formed another group. Mustard and rg-pfl isolates did not seem to have any relationship with these two groups. Taken together, these results indicated that virulence differentiation on host plants, molecular phylogenetic analysis of the nucleotide and the deduced amino acid of TuMV coat proteins did not show any relationship. The multi-resistant lines, Wonyae 20026 and BP058 in Chinese cabbage represent valuable genetic materials that can be used for crucifer breeding programs on TuMV resistance, but not in Korean radish.

Occurrence of Bean common mosaic virus (BCMV) Infecting Peanut in Korea

Choi, Hong-Soo,Kim, Mi-Kyeong,Park, Jin-Woo,Cheon, Jeong-Uk,Kim, Kook-Hyung,Kim, Jeong-Soo,Karakacha Were Hassan,Choi, Jang-Kyung,Takanami Yoichi The Korean Society of Plant Pathology 2006 Plant Pathology Journal Vol.22 No.1

A virus causing vein banding, sometimes yellow mosaic and rugose symptoms on peanut was prevalent around Suwon, Korea. A survey conducted in the area found disease incidence, depending on cultivar, to range from 79 to $100\%$. The virus was found to be seed-transmissible in all the five peanut cultivars tested with transmission rates ranging from 2 to $16\%$. Host range analysis failed to differentiate 9 field isolates collected from different peanuts cultivars showing various symptoms. Inclusion bodies such as scroll, pinwheel and long laminated aggregates induced by the virus in host plant cells were similar to those induced by members of the Potyvirus subdivision III. The virus showed < $95\%$ homology with Bean common mosaic virus (BCMV), BCMV-BICMV/AzMV strains and only < $91\%$ with Desmodium mosaic virus. Based on biological characterization, electron microscopy and molecular analyses of a Korean isolate (Daewon 1), the virus was identified as peanut stripe strain of BCMV.