DNA Methylation Pattern of Gene Promoters of MB-COMT, DRD2, and NR3C1 in Turkish Patients Diagnosed with Schizophrenia

Hasan Mervan Aytac(Hasan Mervan Aytac ),Yasemin Oyaci(Yasemin Oyaci ),Mustafa Pehlivan(Mustafa Pehlivan ),Sacide Pehlivan(Sacide Pehlivan ) 대한정신약물학회 2022 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.20 No.4

Objective: We aim to evaluate the methylation status of membrane-bound catechol-O-methyltransferase (MB-COMT ) promotor, dopamine receptor D2 (DRD2 ), and nuclear receptor subfamily 3 group C member 1 (NR3C1 ) gene in patients with SCZ by comparing healthy controls. Methods: A sample of 110 patients with SCZ and 100 age- and sex-matched healthy volunteers was included in the study. The interview was started by filling out data forms that included sociodemographic and clinical information. The Structured Clinical Interview for DSM-IV Axis I Disorders was used to confirming the diagnosis according to DSM-IV-TR criteria. Then the patients were evaluated with the Positive and Negative Symptoms Scale in terms of symptom severity. Methylation-specific polymerase chain reaction was used to determine the methylation status of MB-COMT promotor, DRD2 , and NR3C1 gene from DNA material. Results: When we compared the percentages of MB-COMT promotor, DRD2 , and NR3C1 gene methylation status in SCZ patients with the healthy control group, the percentages of MB-COMT promotor (OR: 0.466; 95% CI: 0.268− 0.809; p = 0.006), DRD2 (OR: 0.439; 95% CI: 0.375−0.514; p < 0.001), and NR3C1 (OR: 0.003; 95% CI: 0.001− 0.011; p < 0.001) gene methylation status of SCZ was found to be significantly different from the control group. Whereas unmethylation of MB-COMT promotor and NR3C1 genes were associated with SCZ, the partial methylation of the DRD2 gene was related to the SCZ. Conclusion: The MB-COMT promotor, DRD2 , and NR3C1 gene methylation status may be associated with the SCZ in the Turkish population.

Superoxide Dismutase Isoenzyme Activities in Plasma and Tissues of Iraqi Patients with Breast Cancer

Hasan, Hathama Razooki,Mathkor, Thikra Hasan,Al-Habal, Mohammed Hasan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Breast cancer is the first of the most common ten cancers in Iraq. Its etiology is multifactorial, oxidative stress and lipid peroxidation being suggested to play important roles in carcinogenesis. The purpose of this study was to investigate the oxidant-antioxidant status in breast cancer patients, by measuring SOD isoenzyme activities (total SOD, CuZn-SOD, Mn-SOD and EC-SOD) in plasma and breast tumors, and by estimating thiobarbituric reactive substances (TBRS) in tissue homogenates. General increase in total SOD activity was observed in plasma and tissue samples of breast tumors, greater in the malignant when compared to benign group (p<0.05). Mn-SOD showed a significant decrease in tissue malignant samples (p<0.05), and insignificant decrease in plasma malignant samples compared with control and benign samples. Plasma EC-SOD activity in both patient benign and malignant breast tumors demonstrated 3.5% and 22.8% increase, respectively. However, there was a decrease in tissue EC-SOD activity in malignant breast tumors when compared with benign. A similar tendency was noted for TBRS. We suggest that elevated total SOD might reflect a response to oxidative stress, and then may predict a state of excess reactive oxygen species in the carcinogenesis process. If there is proteolytic removal of the heparin binding domain, EC-SOD will lose its affinity for the extracellular matrix and diffuse out of the tissue. This will result in a decreased EC-SOD activity, thus leading to an increase in the steady-state concentration of $O^{2-}$ in this domain, and increase in EC-SOD activity in the extracellular fluid. This might explain the results recorded here concerning the decrease in tissue EC-SOD activity and increase in plasma of breast cancer patients.

Effect of Agricultural Exports and Imports on Economic Growth in Bangladesh: A Study on Agribusiness Supply Chain

Mostofa Mahmud HASAN(Mostofa Mahmud HASAN ),BM Sajjad HOSSAIN(BM Sajjad HOSSAIN ),Md. Abu SAYEM(Md. Abu SAYEM ),Mahnaz AFSAR(Mahnaz AFSAR ) 한국유통과학회 2022 유통과학연구 Vol.20 No.11

Purpose: The purpose of this study was to determine the effect of agricultural exports and imports on economic growth in Bangladesh and propose an upgraded and customized model of the supply chain for agribusiness growth in Bangladesh to achieve plain sailing and systematic operation and financial gains at home and abroad. Research design, data, and methodology: All data in the research have been collected from secondary sources. Gross domestic product was used as the dependent variable and exports and imports of agricultural products were used as independent variables. Pairwise Granger causality was utilized to see the impact of the variable responsible for the economic growth in Bangladesh and the causal relationship between the variables analyzed was measured using Johansen co-integration test. Results: From the empirical analysis, the researchers observed that agricultural commodity imports and exports have a unidirectional impact on economic growth in Bangladesh and a long-run causal link with economic growth in Bangladesh. The suggested supply chain model of agnbusiness aids in achieving smooth operations, systematic management, and monetary gains both domestically and internationally. Conclusions: This paper contnbutes to the development of a more effective and profitable agribusiness supply chain in Bangladesh systematically through their theoretical and practical implications.

Anther Culture in Crop Plants: Progress and Perspectives

M. Thoihidul Islam(M. Thoihidul Islam ),Mohammad Rashid Arif(Mohammad Rashid Arif ),Md. Toufiq Hasan(Md. Toufiq Hasan ),Arif Hasan Khan Robin(Arif Hasan Khan Robin ) 한국육종학회 2023 Plant Breeding and Biotechnology Vol.11 No.2

A resurrection has started in haploid and double haploid research in the twenty-first century. The haploid and double haploid could be achieved through in vivo and in vitro anther and microspore culture techniques. Fixing the homozygosity is the most striking benefit of androgenesis. Various factors like genotypic dependency, growth condition, developmental stage of the microspore, pre-treatment, culture media, regeneration media, growth hormones, and various chemicals have a direct effect. Wheat, rice, Brassica, and tobacco are the notable crops where anther and microspore culture has been utilized. These haploidy and double haploidy through anther culture served many purposes of basic and applied research. Especially, double haploid cultivars have been cultivating around the globe. In addition, for chromosome mapping, QTL mapping, marker-assisted selection, marker-assisted backcrossing, mutation breeding, genome-wide association study, genomic engineering, and genome editing, androgenesis based haploid and double haploid plants have been exploited due to the effectiveness. Recently, researchers are trying to explain albinism that happens during anther culture from an epigenetic perspective. Further prospects of haploid and doubled haploid research through anther culture have been described in this review.

A comprehensive analysis of degree based condition for Hamiltonian cycles

Hasan, Md. Kamrul,Kaykobad, Mohammad,Lee, Young-Koo,Lee, Sungyoung Elsevier 2010 Theoretical computer science Vol.411 No.1

<P><B>Abstract</B></P><P>Since finding whether a graph has a Hamiltonian path or Hamiltonian cycle are both NP-complete problems, researchers have been formulating sufficient conditions that ensure the path or cycle. Rahman and Kaykobad (2005) <ce:cross-ref refid='b2'>[2]</ce:cross-ref> presented a sufficient condition for determining the existence of Hamiltonian path. Three recent works–Lenin Mehedy, Md. Kamrul Hasan, Mohammad Kaykobad (2007) <ce:cross-ref refid='b3'>[3]</ce:cross-ref>, Rao Li (2006) <ce:cross-ref refid='b4'>[4]</ce:cross-ref>, Shengjia Li, Ruijuan Li, Jinfeng Feng (2007) <ce:cross-ref refid='b5'>[5]</ce:cross-ref>–further used the same or similar condition to ensure Hamiltonian cycle with some exceptions. The three works, along with their unique findings, have some common results. This paper unifies the results and brings them under Rahman and Kaykobad’s condition.</P>

Molecular Characterization of Legionellosis Drug Target Candidate Enzyme Phosphoglucosamine Mutase from Legionella pneumophila (strain Paris): An In Silico Approach

Hasan, Md. Anayet,Mazumder, Md. Habibul Hasan,Khan, Md. Arif,Hossain, Mohammad Uzzal,Chowdhury, A.S.M. Homaun Kabir Korea Genome Organization 2014 Genomics & informatics Vol.12 No.4

The harshness of legionellosis differs from mild Pontiac fever to potentially fatal Legionnaire's disease. The increasing development of drug resistance against legionellosis has led to explore new novel drug targets. It has been found that phosphoglucosamine mutase, phosphomannomutase, and phosphoglyceromutase enzymes can be used as the most probable therapeutic drug targets through extensive data mining. Phosphoglucosamine mutase is involved in amino sugar and nucleotide sugar metabolism. The purpose of this study was to predict the potential target of that specific drug. For this, the 3D structure of phosphoglucosamine mutase of Legionella pneumophila (strain Paris) was determined by means of homology modeling through Phyre2 and refined by ModRefiner. Then, the designed model was evaluated with a structure validation program, for instance, PROCHECK, ERRAT, Verify3D, and QMEAN, for further structural analysis. Secondary structural features were determined through self-optimized prediction method with alignment (SOPMA) and interacting networks by STRING. Consequently, we performed molecular docking studies. The analytical result of PROCHECK showed that 95.0% of the residues are in the most favored region, 4.50% are in the additional allowed region and 0.50% are in the generously allowed region of the Ramachandran plot. Verify3D graph value indicates a score of 0.71 and 89.791, 1.11 for ERRAT and QMEAN respectively. Arg419, Thr414, Ser412, and Thr9 were found to dock the substrate for the most favorable binding of S-mercaptocysteine. However, these findings from this current study will pave the way for further extensive investigation of this enzyme in wet lab experiments and in that way assist drug design against legionellosis.

Automatic detection of icing wind turbine using deep learning method

Hasan Basri Başağa,Hasan Basri Başağa,Selen Ayas,Mohammad Tordi Karimi 한국풍공학회 2022 Wind and Structures, An International Journal (WAS Vol.34 No.6

Detecting the icing on wind turbine blades built-in cold regions with conventional methods is always a very laborious, expensive and very difficult task. Regarding this issue, the use of smart systems has recently come to the agenda. It is quite possible to eliminate this issue by using the deep learning method, which is one of these methods. In this study, an application has been implemented that can detect icing on wind turbine blades images with visualization techniques based on deep learning using images. Pre-trained models of Resnet-50, VGG-16, VGG-19 and Inception-V3, which are well-known deep learning approaches, are used to classify objects automatically. Grad-CAM, Grad-CAM++, and Score-CAM visualization techniques were considered depending on the deep learning methods used to predict the location of icing regions on the wind turbine blades accurately. It was clearly shown that the best visualization technique for localization is Score-CAM. Finally, visualization performance analyses in various cases which are close-up and remote photos of a wind turbine, density of icing and light were carried out using Score-CAM for Resnet-50. As a result, it is understood that these methods can detect icing occurring on the wind turbine with acceptable high accuracy

Increasing of Macrophage Migration Inhibitory Factor Expression in Human Patients Infected with Virulent Brucella in Iraq

( Hasan R. Khudhur ),( Abbas Ali Menshed ),( Ahmed Abbas Hasan ) 한국미생물생명공학회(구 한국산업미생물학회) 2020 한국미생물·생명공학회지 Vol.48 No.4

Brucellosis is a zoonotic disease caused by Brucella infections and humans usually contract this disease from close contact with infected animals or their products, usually via the ingestion of cheese or crude milk. Macrophage migration inhibitory factor (MIF) and Pro- and anti-inflammatory cytokines play an important role in susceptibility/resistance and the immunopathogenesis of Brucella infection. These cytokines are crucial factors in the initiation and progression of protective immunity against Brucella infection but the role of MIF has not been well studied in the human response to intracellular microbes. This study was designed to investigate the effect of MIF expression on Brucella susceptibility. A total of 85 positive rose Bengal tests and 24 samples from healthy individuals were collected for this study and subjected to polymerase chain reaction assays (PCR) of the bcsp31 diagnostic gene. MIF concentrations were evaluated using Enzyme-Linked immunosorbent assay (ELISA) and the results showed that 46 (54%) of the rose Bengal test samples were positive and 39 (46%) were negative for bcsp31 (p ≤ 0.05) and used as the gold standard for all of the comparisons in this study. The ELISA results indicate that the mean concentration of MIF was significantly higher in patients with positive rose Bengal tests when compared to the control groups and that its concentration increases with increasing age in both the patient and control groups (p ≤ 0.05).

Issues Related to Employees at Telecommunication Companies in Yemen and the Role of Management Information Systems in Solving It

Yaser Hasan Al-Mamary,Alina Shamsuddin,Nor Aziati Abdul Hamid,Mohammed Hasan Al-Maamari 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.12

In this days, organizations invest in management information systems because they provide economic value to the business. While the adoption of management information systems in the organizations, especially in Yemen is still dealing with issues in field of successful adoption. In other hands, the Telecommunications industry today is a key enabler of productivity across economies and societies. In context of Yemen, the telecommunication sector is a strategic and vital sector being of direct link with the citizen and public and private institutions alike. The sector has witnessed substantial change and developments during the last ten years. However, There are a lot of issues in telecommunication companies in Yemen. This paper aims to shed some light on issues related to employees at telecommunication companies in Yemen, and the role of management information systems in solving these issues.

Estimation of Genetic Parameters of Body Weight Traits in Ghezel Sheep

Baneh, Hasan,Hafezian, Seyed Hasan,Rashidi, Amir,Gholizadeh, Mohsen,Rahimi, Ghodrat Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.2

The present study was carried out to estimate genetic parameters for body weight traits in Ghezel sheep. The data set used was records of 9,221 lambs from 180 sires and 5,060 dams for birth weight (BW), 7,206 lambs from 167 sires and 4,497 dams for weaning weight (WW) and 6,112 lambs from 157 sires and 3,841 dams for 6-months weight (6 MW), which were collected from 1999 to 2007 (9-years) at Ghezel sheep Breeding Station in west Azarbaijan. Variance components and corresponding genetic parameters were obtained with univariate analyses fitting animal models using restricted maximum likelihood (REML) methods. The most suitable model for each trait was determined based on log likelihood ratio tests. Birth year, lamb gender, type of birth, age of dam and herd were significant sources of variation on BW, WW and 6 MW (p<0.01). Direct estimate of heritability for BW, WW and 6 MW was 0.24, 0.29 and 0.37, respectively. The estimate of maternal permanent environmental variance as a proportion of phenotypic variance was 0.09 and 0.05 for BW and WW, respectively. The results of this study showed that genetic progress for growth traits is possible by selection.