Identification and characterization of atypical porcine pestivirus genomes in newborn piglets with congenital tremor in China

Han-Qin Shen,Xiangcong Liu,Pengfei Zhang,Lei Wang,Yan-ling Liu,Le-yi Zhang,Peng-shuai Liang,Chang-xu Song 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.3

Recently, a novel atypical porcine pestivirus (APPV) in pig was reported. In this study, two APPV strains, APPV-China/GZ01/2016 (GZ01) and APPV-China/GD-SD/2016 (GD-SD), were identified in two newborn piglet herds with congenital tremor from China. The open reading frame of the two strains shared an 83.5% nucleotide identity. Phylogenetically, the APPV strains were placed into two groups: GZ01 belonged to group I and GD-SD belonged to group II. A high viral load was detected in the cerebellum (quantification cycles < 26). Further studies should be carried out to thoroughly elucidate the development of congenital tremors caused by APPV.

Rubrofusarin-6-β-gentiobioside inhibits lipid accumulation and weight gain by regulating AMPK/mTOR signaling

Han, Yo-Han,Kee, Ji-Ye,Park, Seong-Hwan,Mun, Jeong-Geon,Jeon, Hee-Dong,Park, Jinbong,Zou, Qin-Peng,Liu, Xiang-Qian,Hong, Seung-Heon Elsevier 2019 Phytomedicine Vol.62 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Although rubrofusarin-6-β-gentiobioside (RFG), which is a component of <I>Cassiae tora</I> seed, could likely regulate hyperlipidemia, its anti-obesity effect and related mechanism have not been elucidated.</P> <P><B>Purpose</B></P> <P>The aim of this study was to examine whether RFG can ameliorate obesity and the mechanism of lipid accumulation regulated by RFG.</P> <P><B>Study design</B></P> <P>In <I>in vitro</I> experiments, we confirmed the anti-adipogenic effect of RFG using 3T3-L1 cells and human adipose mesenchymal stem cells (hAMSCs). To confirm the anti-obesity effect, High-Fat Diet (HFD)-induced obese mice were selected as a model.</P> <P><B>Methods</B></P> <P>We investigated anti-adipogenic effects of RFG using MTS assay, Oil Red O Staining, real-time RT-PCR, western blot analysis, and immunofluorescence staining. The anti-obesity effect of RFG was confirmed in HFD-induced mice model using hematoxylin and eosin staining and serum analysis.</P> <P><B>Results</B></P> <P>RFG inhibited lipid accumulation in 3T3-L1 cells and hAMSCs by reducing expression of mammalian targets of rapamycin (mTOR), peroxisome proliferator-activated receptor (PPAR)γ, and CCAAT-enhancer binding protein (C/EBP)α. RFG phosphorylated AMP-activated protein kinase (AMPK) in a liver kinase B (LKB) 1-independent manner. Moreover, the anti-adipogenic effect of RFG was blocked by AMPK inhibitor. These results suggest that RFG inhibits lipid accumulation via AMPK signaling. Furthermore, RFG reduced the body weight, size of epididymal white adipose tissue (eWAT), and fatty liver in the mice. RFG also suppressed levels of adipogenic factors PPARγ, C/EBPα, FAS, LPL, and aP2) by activating AMPK in the eWAT and liver.</P> <P><B>Conclusion</B></P> <P>RFG can ameliorate obesity, and thus, could be used as a therapeutic agent for treating obesity.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Isolation and phylogenetic analysis of hemagglutinin gene of H9N2 influenza viruses from chickens in South China from 2012 to 2013

Han-Qin Shen,Zhuan-Qiang Yan,Fan-Gui Zeng,Chang-Tao Liao,Qing-Feng Zhou,Jian-Ping Qin,Qingmei Xie,Yingzuo Bi,Feng Chen 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.3

As part of our ongoing influenza surveillance program in South China, 19 field strains of H9N2 subtype avian influenza viruses (AIVs) wereisolated from dead or diseased chicken flocks in Guangdong province, South China, between 2012 and 2013. Hemagglutinin (HA) genes ofthese strains were sequenced and analyzed and phylogenic analysis showed that 12 of the 19 isolates belonged to the lineage h9.4.2.5, whilethe other seven belonged to h9.4.2.6. Specifically, we found that all of the viruses isolated in 2013 belonged to lineage h9.4.2.5. The lineageh9.4.2.5 viruses contained a PSRSSR↓GLF motif at HA cleavage site, while the lineage h9.4.2.6 viruses contained a PARSSR↓GLF at thesame position. Most of the isolates in lineage h9.4.2.5 lost one potential glycosylation site at residues 200–202, and had an additional oneat residues 295–297 in HA1. Notably, 19 isolates had an amino acid exchange (Q226L) in the receptor binding site, which indicated that theviruses had potential affinity of binding to human like receptor. The present study shows the importance of continuing surveillance of newH9N2 strains to better prepare for the next epidemic or pandemic outbreak of H9N2 AIV infections in chicken flocks.

A Survey on the Outsourcing Usage of Logistics Service Users in China

Qin-Qin Fu,Jung-Han Bae(배정한),Gwi-Ok Kim(김귀옥) 한국항만경제학회 2009 韓國港灣經濟學會誌 Vol.25 No.1

본 논문은 중국 상해를 중심으로 로지스틱 서비스를 이용하는 기업들의 로지스틱 아웃소싱 실태에 대한 설문조사를 통하여 중국의 물류아웃소싱 관행에 대한 조사연구이다. 중국 상해에 소재하는 158개 제조기업을 대상으로 설문조사를 했다. 연구 결과는 첫째, 전형적 물류를 아웃소싱 하는 제조기업들은 서비스 제공업자들과 장기적 관계를 유지하고 또한 다수의 물류서비스업자들로부터의 물류서비스 구매를 선호했다. 둘째, 대부분의 서비스 이용기업들은 물류서비스 제공기업들에 대하여 만족했고, 물류기업들에게 자사의 물류를 아웃소싱 할 때, 자사의 고객만족도, 물류 수준과 수익개선에 긍정적 영향을 미치는 것으로 나타났다. 셋째, 물류서비스를 이용하는 제조기업들은 물류기업을 선택할 때 가격수준, 핵심적 경쟁력과 평판을 가장 중요한 요인으로 하는 것으로 나타났다. 넷째, 중국에서 3PL 등의 물류전문서비스 사업이 초기단계이지만, 앞으로 발전 잠재력이 매우 큰 것으로 조사되었다. 따라서 본 논문은 상해를 중심으로 중국에서 영업을 하고 중국 국내물류기업 또는 우리나라 투자기업을 포함한 외자물류기업들에게 물류서비스 이용기업들의 물류 아웃소싱 실태와 관행에 대한 정보를 제공함으로 물류서비스산업의 발전에 도움이 될 것으로 사료된다. This paper analyses the results of the survey questionnaires which are made for logistics services users in Shanghai, China. Based on 158 valid users' responses, the study results show that the typical outsourcing logistics users prefer to set up long term relationship with providers and buy services from multiple providers. Most users are satisfied with their providers and believe that outsourcing logistics services has led to positive impact on their customer satisfaction, logistics service levels and revenue improvement. Price levers, core competences, reputation are critical in the selection of an outsourcing logistics provider for users in this study. This study indicates that the market for 3PL services in China has a reasonable potential for further development, though 3PL practices are still at a nascent stage in China. This paper presents full details and implications of the results of the survey and then provides some helpful suggestion for the development of Chinese logistics industry and Chinese logistics companies.

農業觀光園空間硏究 -水上娛樂空間遊客行爲心理特點與對其評價-

한소근 ( Su Qin Han ),박영길 ( Yong Ji Piao ),안승원 ( Seoung Won Ann ) 한국녹지환경디자인학회 2007 학술발표회 Vol.2007 No.-

農業觀光園空間硏究 -水上娛樂空間游客行爲心理特点與對其評价-

한소근 ( Su Qin Han ),박영길 ( Yong Ji Piao ),안승원 ( Seoung Won Ann ) 한국녹지환경디자인학회 2007 녹지환경학회지 Vol.3 No.3

Investigation of the recreation space on water in sightseeing agricultural park is the object of this study. The behavioral and mental characteristic of tourist with different age and their estimation to landscape was investigated by research methods such as behavior observation, accessing and questionnaire investigation on spot, which may give an advice on design of space on water in sightseeing agricultural park.

Behavior of improved through-diaphragm connection to square tubular column under tensile loading

Qin, Ying,Zhang, Jing-Chen,Shi, Peng,Chen, Yi-Fu,Xu, Yao-Han,Shi, Zuo-Zheng 국제구조공학회 2018 Structural Engineering and Mechanics, An Int'l Jou Vol.68 No.4

Square tubular columns are commonly used in moment resisting frames, while through-diaphragm connection is the most typical configuration detail to connect the H-shaped beam to the column. However, brittle fracture normally occurs at the complete joint penetration weld between the beam flange and the through-diaphragm due to the stress concentration caused by the geometrical discontinuity. Accordingly, three improved types of through-diaphragm are presented in this paper to provide smooth force flow path comparing to that of conventional connections. Tensile tests were conducted on four specimens and the results were analyzed in terms of failure modes, load-displacement response, yield and ultimate capacity, and initial stiffness. Furthermore, strain distributions on the through-diaphragm, the beam flange plate, and the column face were comprehensively evaluated and discussed. It was found that all the proposed three types of improved through-diaphragm connections were able to reduce the stress concentration in the welds between the beam flange and the through-diaphragm. Furthermore, the stress distribution in connection with longer tapered through-diaphragm was more uniform.

Radixin Knockdown by RNA Interference Suppresses Human Glioblastoma Cell Growth in Vitro and in Vivo

Qin, Jun-Jie,Wang, Jun-Mei,Du, Jiang,Zeng, Chun,Han, Wu,Li, Zhi-Dong,Xie, Jian,Li, Gui-Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Radixin, a member of the ERM (ezrin-radixin-moesin) family, plays important roles in cell motility, invasion and tumor progression. It is expressed in a variety of normal and neoplastic cells, including many types of epithelial and lymphoid examples. However, its function in glioblastomas remains elusive. Thus, in this study, radixin gene expression was first examined in the glioblastoma cells, then suppressed with a lentivirus-mediated short-hairpin RNA (shRNA) method.We found that there were high levels of radixin expression in glioblastoma U251cells. Radixin shRNA caused down-regulation of radixin gene expression and when radixin-silenced cells were implanted into nude mice, tumor growth was significantly inhibited as compared to blank control cells or nonsense shRNA cells. In addition, microvessel density in the tumors was significantly reduced. Thrombospondin-1 (TSP-1) and E-cadherin were up-regulated in radixin- suppressed glioblastoma U251 cells. In contrast, MMP9 was down-regulated. Taken together, our findings suggest that radixin is involved in GBM cell migration and invasion, and implicate TSP-1, E-cadherin and MMP9 as metastasis-inducing factors.

Diffusion-Weighted Imaging for the Left Hepatic Lobe has Higher Diagnostic Accuracy for Malignant Focal Liver Lesions

Han, Xue,Dong, Yin,Xiu, Jian-Jun,Zhang, Jie,Huang, Zhao-Qin,Cai, Shi-Feng,Yuan, Xian-Shun,Liu, Qing-Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: This study was conducted to investigate whether apparent diffusion coefficient (ADC) measurements by dividing the liver into left and right hepatic lobes may be utilized to improve the accuracy of differential diagnosis of benign and malignant focal liver lesions. Materials and Methods: A total of 269 consecutive patients with 429 focal liver lesions were examined by 3-T magnetic resonance imaging that included diffusion-weighted imaging. For 58 patients with focal liver lesions of the same etiology in left and right hepatic lobes, ADCs of normal liver parenchyma and focal liver lesions were calculated and compared using the paired t-test. For all 269 patients, ADC cutoffs for focal liver lesions and diagnostic accuracy in the left hepatic lobe, right hepatic lobe and whole liver were evaluated by receiver operating characteristic curve analysis. Results: For the group of 58 patients, mean ADCs of normal liver parenchyma and focal liver lesions in the left hepatic lobe were significantly higher than those in the right hepatic lobe. For differentiating malignant lesions from benign lesions in all patients, the sensitivity and specificity were 92.6% and 92.0% in the left hepatic lobe, 94.4% and 94.4% in the right hepatic lobe, and 90.4% and 94.7% in the whole liver, respectively. The area under the curve of the right hepatic lobe, but not the left hepatic lobe, was higher than that of the whole liver. Conclusions: ADCs of normal liver parenchyma and focal liver lesions in the left hepatic lobe were significantly higher than those in the right hepatic lobe. Optimal ADC cutoff for focal liver lesions in the right hepatic lobe, but not in the left hepatic lobe, had higher diagnostic accuracy compared with that in the whole liver.

Multi-functional transparent electrode for reliable flexible perovskite solar cells

Han, Gill Sang,Lee, Seongha,Duff, Matthew Lawrence,Qin, Fen,Jiang, Minlin,Li, Guangyong,Lee, Jung-Kun Elsevier 2019 Journal of Power Sources Vol.435 No.-

<P><B>Abstract</B></P> <P>Multilayer MoO<SUB>x</SUB>/Ag/MoO<SUB>x</SUB> (DMD) films are found to be transparent conducting electrodes for use in extremely stable and highly bendable flexible perovskite solar cells (PSCs). The optical transparency and electric properties of DMD and its role as a top electrode of PSCs were studied by changing the thickness of the MoO<SUB>x</SUB> layer. Although the MoO<SUB>x</SUB> thickness was shown to have a negligible effect on the sheet resistance of DMD, the transmittance of visible light, selective carrier transport capability, and long-term stability of a device considerably depend on this factor. The sandwich structure of a 20-nm-thick MoO<SUB>x</SUB>, 7-nm-thick Ag, and 20-nm-thick MoO<SUB>x</SUB> exhibits a high transmittance and large photon–electron conversion rate of PSCs. In addition, PSCs using the DMD top electrode maintain 92% of their initial current density after 24 h of continuous operation owing to a UV light cut-off of the top illumination. Moreover, the overall structure of DMD blocks the diffusion of water and oxygen molecules from real environmental conditions. At the same time, the underlying/upper MoO<SUB>x</SUB> layer retards the degradation through a chemical reaction between Ag and the halide ions inside the cells, as well as foreign ions from outside the polluted atmosphere. When DMD is applied to flexible PSCs on Ti foil, the PCE reaches 14.5%, and mechanical integrity of the PSCs is maintained at a bending radius of 4 mm.</P> <P><B>Highlights</B></P> <P> <UL> <LI> MoO<SUB>x</SUB>/Ag/MoO<SUB>x</SUB> multilayer is used as a top electrode of perovskite solar cells. </LI> <LI> The multilayer electrode has multiple functions in real operation environment. </LI> <LI> 92% of the initial current density is maintained after 24 h continuous operation. </LI> <LI> The efficiency of flexible solar cells using MoO<SUB>x</SUB>/Ag/MoO<SUB>x</SUB> reaches 14.5%. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>