LV Remodeling after MI is Mediated by the Interaction of Mast Cells and Accompanying Cardiomyocytes Regeneration

( Hainan Piao ),( Jin Sook Kwon ),( Sang Yeub Lee ),( Jang Whan Bae ),( Kyung Kuk Hwang ),( Tae Jin Youn ),( Dong Woon Kim ),( Myeong Chan Cho ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.4

Mast cells perform the pivotal role in various allergic diseases, immunologic and inflammatory processes through the production of cytokine, protease and histamine. We investigated that the role of mast cells in the aspect of cardiomyocyte regeneration and left ventricular remodeling process during early and late period in acute myocardial infarction model in rat. Apoptosis/survival of cardiomyocyte in the serial concentration condition of mast cell granule was observed in vitro. In the rat myocardial infarction tissue, the association between actively dividing or Ki-67 positive cardiomyocyte and mast cell population was investigated. Mast cell action to the ventricular remodeling process after myocardial infarction was measured. Cardiomyocyte apoptosis, not necrosis by the mast cell granule was evident in concentration dependent manner. Mast cell density is the highest in 14 days after the myocardial infarction and abundant number of mast cell was observed in infarct-border area than infarct or remote area. Mast cell affects not only in the cardiomyocyte apoptosis and regeneration in the early stage but also in ventricular remodeling process in the late stage of myocardial infarction.

Robust Object Tracking with Occlusion Handling based on Local Sparse Representation

Hainan Zhao,Xuan Wang,Meng Liu 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.3

The Robust Derivative Code for Object Recognition

( Hainan Wang ),( Baochang Zhang ),( Hong Zheng ),( Yao Cao ),( Zhenhua Guo ),( Chengshan Qian ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.1

This paper proposes new methods, named Derivative Code (DerivativeCode) and Derivative Code Pattern (DCP), for object recognition. The discriminative derivative code is used to capture the local relationship in the input image by concatenating binary results of the mathematical derivative value. Gabor based DerivativeCode is directly used to solve the palmprint recognition problem, which achieves a much better performance than the state-of-art results on the PolyU palmprint database. A new local pattern method, named Derivative Code Pattern (DCP), is further introduced to calculate the local pattern feature based on Dervativecode for object recognition. Similar to local binary pattern (LBP), DCP can be further combined with Gabor features and modeled by spatial histogram. To evaluate the performance of DCP and Gabor-DCP, we test them on the FERET and PolyU infrared face databases, and experimental results show that the proposed method achieves a better result than LBP and some state-of-the-arts.

Porcine growth hormone induces the nuclear localization of porcine growth hormone receptor in vivo

Lan, Hainan,Liu, Huilin,Hong, Pan,Li, Ruonan,Zheng, Xin Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.4

Objective: Recent studies have challenged the traditional paradigm that growth hormone receptor (GHR) displays physiological functions only in the cell membrane. It has been demonstrated that GHR localizes to the cell nucleus and still exhibits important physiological roles. The phenomenon of nuclear localization of growth hormone (GH)-induced GHR has previously been described in vitro. However, until recently, whether GH could induce nuclear localization of GHR in vivo was unclear. Methods: In the present study, we used pig as an animal model, and porcine growth hormone (pGH) or saline was injected into the inferior vena cava. We subsequently observed the localization of porcine growth hormone receptor (pGHR) using multiple techniques, including, immunoprecipitation and Western-blotting, indirect immunofluorescence assay and electronmicroscopy. Results: The results showed that pGH could induce nuclear localization of pGHR. Taken together, the results of the present study provided the first demonstration that pGHR was translocated to cell nuclei under pGH stimulation in vivo. Conclusion: Nuclear localization of pGHR induced by the in vivo pGH treatment suggests new functions and/or novel roles of nuclear pGHR, which deserve further study.

Solid-phase based on-chip DNA purification through a valve-free stepwise injection of multiple reagents employing centrifugal force combined with a hydrophobic capillary barrier pressure

Zhang, Hainan,Tran, Hong Hanh,Chung, Bong Hyun,Lee, Nae Yoon The Royal Society of Chemistry 2013 The Analyst Vol.138 No.6

<P>In this paper, we demonstrate a simple technique for sequentially introducing multiple sample liquids into microchannels driven by centrifugal force combined with a hydrophobic barrier pressure and apply the technique for performing solid-phase based on-chip DNA purification. Three microchannels with varying widths, all equipped with independent sample reservoirs at the inlets, were fabricated on a hydrophobic elastomer, poly(dimethylsiloxane) (PDMS). First, glass beads were packed inside the reaction chamber, and a whole cell containing the DNA extract was introduced into the widest channel by applying centrifugal force for physical adsorption of the DNA onto the glass beads. Next, washing and elution solutions were sequentially introduced into the intermediate and narrowest microchannels, respectively, by gradually increasing the amount of centrifugal force. Through a precise manipulation of the centrifugal force, the DNA adsorbed onto the glass beads was successfully washed and eluted in a continuous manner without the need to introduce each solution manually. A stepwise injection of liquids was successfully demonstrated using multiple ink solutions, the results of which corresponded well with the theoretical analyses. As a practical application, the D1S80 locus of human genomic DNA, which is widely used for forensic purposes, was successfully purified using the microdevice introduced in this study, as demonstrated through successful target amplification. This will pave the way for the construction of a control-free valve system for realizing on-chip DNA purification, which is one of the most labor-intensive and hard-to-miniaturize components, on a greatly simplified and miniaturized platform employing hydrophobic PDMS.</P> <P>Graphic Abstract</P><P>Centrifugal force combined with a hydrophobic capillary barrier pressure on a PDMS microdevice ensures a stepwise injection of multiple reagents in a valve-free manner, applicable for solid-phase based on-chip DNA purification. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3an36409g'> </P>

Ternary Phase Diagram-Facilitated Rapid Screening of Double Perovskites As Electrocatalysts for the Oxygen Evolution Reaction

Sun, Hainan,Hu, Zhiwei,Xu, Xiaomin,He, Juan,Dai, Jie,Lin, Hong-Ji,Chan, Ting-Shan,Chen, Chien-Te,Tjeng, Liu Hao,Zhou, Wei,Shao, Zongping American Chemical Society 2019 Chemistry of materials Vol.31 No.15

<P>The development of cost-effective, non-noble metal electrocatalysts for the oxygen evolution reaction (OER) is of paramount importance for sustainable technologies. Efficient screening strategies for electrocatalysts can greatly increase the commercialization speed of these advanced technologies. Here, ternary phase diagrams with large-scale tuning and designated-scale tuning strategies are applied for the first time to provide a new method for screening perovskite oxide-based electrocatalysts for OERs. Specifically, the family of double perovskites (Sr<SUB>2</SUB>Fe<SUB>1.5-<I>x</I>-<I>y</I></SUB>Co<SUB><I>x</I></SUB>Ni<SUB><I>y</I></SUB>Mo<SUB>0.5</SUB>O<SUB>6−δ</SUB>, 0 ≤ <I>x</I>, <I>y</I> ≤ 1.5) was utilized to understand the role of transition metals in perovskite oxides. Ternary phase diagrams can facilitate a rapid screening process, provide a straightforward relationship between phase structures and catalytic activities, and help to confirm the effects of various combinations of transition metals on the OER activity. The Fe-Co system (Sr<SUB>2</SUB>Fe<SUB>1.5-<I>x</I></SUB>Co<SUB><I>x</I></SUB>Mo<SUB>0.5</SUB>O<SUB>6−δ</SUB>) improves the catalytic activities, as demonstrated by the reduced Tafel slope and enhanced stability, while the Fe-Ni system (Sr<SUB>2</SUB>Fe<SUB>1.5-<I>y</I></SUB>Ni<SUB><I>y</I></SUB>Mo<SUB>0.5</SUB>O<SUB>6−δ</SUB>) improves the surface kinetic properties of the OER, as demonstrated by its reduced overpotential. Significantly, the Co, Ni, and Fe ternary phase systems can serve as the synergistic coactive sites (Sr<SUB>2</SUB>Fe<SUB>1.5-<I>x</I>-<I>y</I></SUB>Co<SUB><I>x</I></SUB>Ni<SUB><I>y</I></SUB>Mo<SUB>0.5</SUB>O<SUB>6−δ</SUB>) to catalyze the OER, resulting in an improved overall OER performance. This systematic study not only demonstrates a new strategy to allow the rapid screening of double perovskite OER catalysts based on large-scale tuning and designated-scale tuning strategies but, more importantly, also provides an insightful understanding of the use of multitransition metal-based double perovskites for catalysis of the OER.</P> [FIG OMISSION]</BR>

Boosting the oxygen evolution reaction activity of a perovskite through introducing multi-element synergy and building an ordered structure

Sun, Hainan,Xu, Xiaomin,Hu, Zhiwei,Tjeng, Liu Hao,Zhao, Jie,Zhang, Qin,Lin, Hong-Ji,Chen, Chien-Te,Chan, Ting-Shan,Zhou, Wei,Shao, Zongping The Royal Society of Chemistry 2019 Journal of Materials Chemistry A Vol.7 No.16

<P>If different active sites in a catalyst have optimal binding to different reaction intermediates and short reaction paths among them, they may work cooperatively to enhance the oxygen evolution reaction (OER) activity. Based on this design principle, in this study, we start with a B-site ordered double perovskite Sr2FeMoO6−δ with poor OER activity as the host material to fulfill the requirement of a short pathway, and then, replace Mo with Ni and Fe with Co to optimize the synergistic interplay of the multi-active sites. Replacing Mo with Ni indeed dramatically enhances the OER activity and structural/operating stability. Further improvement in OER performance is realized by partial substitution of Fe with Co, leading to the development of a material with the nominal composition of Sr2Fe0.8Co0.2Mo0.65Ni0.35O6−δ, which outperforms the noble metal oxide IrO2 and is better than most of the electrocatalysts developed based on a single descriptor, such as Ba0.5Sr0.5Co0.8Fe0.2O3−δ (eg occupancy close to unity), PrBaCo2O5+δ (O 2p-band center relative to the Fermi level), and La0.5Sr0.5CoO3−δ (charge-transfer energy) in many aspects. As a universal method, combined structural and compositional tuning to create a cooperative effect among different active sites for intermediate adsorption and reaction in an ordered structure may provide a new way for the design of superior electrocatalysts for various applications.</P>

Stability of Aperiodic Sampled-data Systems - An Augmented Looped Lyapunov Functional with Wirtinger Inequality Approach

Min Zheng,Hainan Yang,Yang Song,Jing Deng,Minrui Fei 제어·로봇·시스템학회 2015 International Journal of Control, Automation, and Vol.13 No.3

This paper is concerned with stability analysis of sampled-data systems with non-uniform sampling patterns. The stability problem is solved based on an augmented looped Lyapunov functional with Wirtinger inequality approach. The effectiveness of this approach depends on proper selecting looped Lyapunov functional parts and less conservative inequality techniques. The method developed here is an extension of previous results by using novel augmented functional and Wirtinger inequality. Numerical examples are given to illustrate the result.

Energy Priority and Current Model Based AUV Cruise Path Planning Algorithm

Guangcong Liu,Hainan Chen,Xiaoling Wu,Dong Li,Tingting Huang 보안공학연구지원센터 2013 International Journal of Multimedia and Ubiquitous Vol.8 No.5

Fabrication of Polymerase Chain Reaction Plastic Lab-on-a-Chip Device for Rapid Molecular Diagnoses

Kieu The Loan Trinh,Hainan Zhang,강동진,강성현,Ben D. Tall,이내윤 대한배뇨장애요실금학회 2016 International Neurourology Journal Vol.20 No.S1

Purpose: We aim to fabricate a thermoplastic poly(methylmethacrylate) (PMMA) Lab-on-a-Chip device to perform continuous- flow polymerase chain reactions (PCRs) for rapid molecular detection of foodborne pathogen bacteria. Methods: A miniaturized plastic device was fabricated by utilizing PMMA substrates mediated by poly(dimethylsiloxane) interfacial coating, enabling bonding under mild conditions, and thus avoiding the deformation or collapse of microchannels. Surface characterizations were carried out and bond strength was measured. The feasibility of the Lab-on-a-Chip device for performing on-chip PCR utilizing a lab-made, portable dual heater was evaluated. The results were compared with those obtained using a commercially available thermal cycler. Results: A PMMA Lab-on-a-Chip device was designed and fabricated for conducting PCR using foodborne pathogens as sample targets. A robust bond was established between the PMMA substrates, which is essential for performing miniaturized PCR on plastic. The feasibility of on-chip PCR was evaluated using Escherichia coli O157:H7 and Cronobacter condimenti, two worldwide foodborne pathogens, and the target amplicons were successfully amplified within 25 minutes. Conclusions: In this study, we present a novel design of a low-cost and high-throughput thermoplastic PMMA Lab-on-a- Chip device for conducting microscale PCR, and we enable rapid molecular diagnoses of two important foodborne pathogens in minute resolution using this device. In this regard, the introduced highly portable system design has the potential to enable PCR investigations of many diseases quickly and accurately.