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Promoted Growth of Maize by the Phosphate Solubilizing Bacteria Isolated from North-east China
Hai-Yan Wu,Li-Chun Wang,Xing-Ai Gao,Rong-De Jin,Zuo-Wei Fan,Kil-Yong Kim,Lan-Po Zhao 한국토양비료학회 2011 한국토양비료학회지 Vol.44 No.1
A strain of phosphate solubilizing bacterium was isolated from rhizosphere and identified as Burkholderia sp. by 16S-rRNA gene sequence analyses. The bacterium was found to release gluconic acid and the solubilization of hydroxyapatite in the liquid medium by a significant drop in pH to 3.7 from an initial pH 7.0. The soluble-P concentration continuously increased during the incubation periods and the total amount of soluble P released in culture filtrate was detected at 990 mg L<SUP>-1</SUP> after 10 days of inoculation. Most promoted maize growth was found in the standard NPK (240-120-120 kg ha<SUP>-1</SUP>) soil inoculation with Burkholderia sp. (Twenty milliliters/ plant, 106 CFU) and also in the absence of Burkholderia sp. inoculation, the soil amended with only 2/3 levels of P gave significant higher plant yield compared to 1/3 levels of P or without P supplementation.
First Report of an Unrecorded Nematode-Trapping Fungus Species Monacrosporium phymatopagum in Korea
Wu, Hai-Yan,Kim, Dong-Geun The Korean Society of Plant Pathology 2010 Plant Pathology Journal Vol.26 No.3
A fungus that can capture nematodes by means of sessile adhesive knobs was isolated from rotten wood in Uiseong, Korea. It was found to produce single, spindle-shaped, 3-4 septate (commonly 4-septate) conidia, $44.8\;{\mu}m$ (range, $41.6-50.1\;{\mu}m$) long and $13.3\;{\mu}m$ (range, $10.7-15.4\;{\mu}m$) wide. Conidiophores were found to be hyaline, erect, straight, and $202.7-245.7\;{\mu}m$ high. On the basis of these morphological features, the fungus was identified as Monacrosporium phymatopagum. This is the first report of M. phymatopagum in Korea which can be a potential biological control resource of plant parasitic nematode.
Hai Ming Huang,Yan Liu,Denghao Wu,Yue Zhong Wu,Tian Xin Wu 한국유체기계학회 2023 International journal of fluid machinery and syste Vol.16 No.1
Cavitation in the pump induces vibration and noise, which leads to the degradation of pump performance and damage to the impeller. Therefore, it is of great significance to accurately identify the cavitation state of the centrifugal pump. To determine the cavitation state using the vibration characteristic frequency of the centrifugal pump, it is necessary to accurately identify the characteristic frequency due to the noise's effect. Therefore, a cavitation fault diagnosis method of centrifugal pump based on a cohesive hierarchy algorithm was proposed. Firstly, Singular Value Decomposition (SVD) is used to de-noise the vibration signal. Then the root means square of the vibration signal after de-noising is obtained as the eigenvalue of the vibration signal. Secondly, the hierarchical clustering algorithm is used to classify the vibration eigenvalues and accurately identify the non-cavitation and cavitation states of centrifugal pumps. Finally, through experimental tests, the method can effectively and quickly identify the cavitation state of the centrifugal pump, with an accuracy of 95%. The study provides a new way for rapidly diagnosing centrifugal pump cavitation
First Report of an Unrecorded Nematode-Trapping Fungus Species Monacrosporium phymatopagum in Korea
Hai-Yan Wu,김동근 한국식물병리학회 2010 Plant Pathology Journal Vol.26 No.3
A fungus that can capture nematodes by means of sessile adhesive knobs was isolated from rotten wood in Uiseong, Korea. It was found to produce single, spindleshaped,3-4 septate (commonly 4-septate) conidia, 44.8μm (range, 41.6-50.1 μm) long and 13.3 μm (range, 10.7-15.4 μm) wide. Conidiophores were found to be hyaline,erect, straight, and 202.7-245.7 μm high. On the basis of these morphological features, the fungus was identified as Monacrosporium phymatopagum. This is the first report of M. phymatopagum in Korea which can be a potential biological control resource of plant parasitic nematode.
Promoted Growth of Maize by the Phosphate Solubilizing Bacteria Isolated from North-east China
Wu, Hai-Yan,Wang, Li-Chun,Gao, Xing-Ai,Jin, Rong-De,Fan, Zuo-Wei,Kim, Kil-Yong,Zhao, Lan-Po Korean Society of Soil Science and Fertilizer 2011 한국토양비료학회지 Vol.44 No.1
A strain of phosphate solubilizing bacterium was isolated from rhizosphere and identified as Burkholderia sp. by 16S-rRNA gene sequence analyses. The bacterium was found to release gluconic acid and the solubilization of hydroxyapatite in the liquid medium by a significant drop in pH to 3.7 from an initial pH 7.0. The soluble-P concentration continuously increased during the incubation periods and the total amount of soluble P released in culture filtrate was detected at 990 mg $L^{-1}$ after 10 days of inoculation. Most promoted maize growth was found in the standard NPK (240-120-120 kg $ha^{-1}$) soil inoculation with Burkholderia sp. (Twenty milliliters/plant, 106 CFU) and also in the absence of Burkholderia sp. inoculation, the soil amended with only 2/3 levels of P gave significant higher plant yield compared to 1/3 levels of P or without P supplementation.
Wu, Bing-Li,Zou, Hai-Ying,Lv, Guo-Qing,Du, Ze-Peng,Wu, Jian-Yi,Zhang, Pi-Xian,Xu, Li-Yan,Li, En-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5
Lysyl oxidase-like 2 (LOXL2), a member of the lysyl oxidase (LOX) family, is a copper-dependent enzyme that catalyzes oxidative deamination of lysine residues on protein substrates. LOXL2 was found to be overexpressed in esophageal squamous cell carcinoma (ESCC) in our previous research. We later identified a LOXL2 splicing variant LOXL2-delta72 and we overexpressed LOXL2-delta72 and its wild type counterpart in ESCC cells following microarray analyses. First, the differentially expressed genes (DEGs) of LOXL2 and LOXL2-delta72 compared to empty plasmid were applied to generate protein-protein interaction (PPI) sub-networks. Comparison of these two sub-networks showed hundreds of different proteins. To reveal the potential specific roles of LOXL2- delta72 compared to its wild type, the DEGs of LOXL2-delta72 vs LOXL2 were also applied to construct a PPI sub-network which was annotated by Gene Ontology. The functional annotation map indicated the third PPI sub-network involved hundreds of GO terms, such as "cell cycle arrest", "G1/S transition of mitotic cell cycle", "interphase", "cell-matrix adhesion" and "cell-substrate adhesion", as well as significant "immunity" related terms, such as "innate immune response", "regulation of defense response" and "Toll signaling pathway". These results provide important clues for experimental identification of the specific biological roles and molecular mechanisms of LOXL2-delta72. This study also provided a work flow to test the different roles of a splicing variant with high-throughput data.
( Hai Xiao Wang ),( Kuang Jie Wu ),( Yuan Sun ),( Yan Dong Li ),( Ming Yu Wu ),( Qian Qiao ),( Yuan Jiang Wei ),( Ze Guang Han ),( Bing Cai ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.11
The human glycoprotein, stanniocalcin 2 (STC2) plays multiple roles in several tumor types, however, its function and clinical significance in hepatocellular carcinoma (HCC) remain unclear. In this study, we detected STC2 expression by quantitative real-time PCR and found STC2 was upregulated in HCC tissues, correla ed with tumor size and multiplicity of HCC. Ectopic expression of STC2 markedly promoted HCC cell proliferation and colony formation, while silencing of endogenous STC2 resulted in a reduced cell growth by cell cycle delay in G0/G1 phase. Western blot analysis demonstrated that STC2 could regulate the expression of cyclin D1 and activate extracellular signal-regulated kinase 1/2 (ERK1/2) in a dominant-positive manner. Transwell chamber assay also indicated altered patterns of STC2 expression had an important effect on cell migration. Our findings suggest that STC2 functions as a potential oncoprotein in the development and progression of HCC as well as a promising molecular target for HCC therapy.
Yan-Ni Wu,Hai-Fu Guo,Peng Hu,Xiao-Peng Xiao,Zhao-Wang Xiao,Shi-Jun Liao 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.7
Three types of ternary low-platinum nanocatalysts, alloy PdPtIr/C, core–shell PdPt@PtIr/C and Pd@PtIr/C, have been prepared, and their catalytic behaviors toward methanol oxidation reaction (MOR)/oxygen reduction reaction (ORR) are comparatively investigated via cyclic voltammetry and chronoamperometry analysis in an acidic medium. Through a two-step colloidal technique, the synthesized core–shell structured catalyst PtPd@PtIr/C with alloy core and alloy shell show the best catalytic activity toward MOR and the best poisoning tolerance. The alloy PdPtIr/C catalyst prepared via a one-step colloidal technique exhibits the best performance toward ORR among the three catalysts. All the three catalysts are characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and other characterization techniques.
Wu, Xiao-Feng,Song, Hai-Yan,Yoon, Jeong-Mo,Yu, Yeon-Tae,Chen, Yun-Fa American Chemical Society 2009 Langmuir Vol.25 No.11
<P>Core−shell Au@TiO<SUB>2</SUB> nanoparticles with truncated wedge-shaped TiO<SUB>2</SUB> morphology have been synthesized successfully by a simple and flexible hydrothermal route. Morphological evolution of TiO<SUB>2</SUB> shells was investigated by transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), and X-ray diffraction technique. It has been revealed that the truncated wedge-shaped TiO<SUB>2</SUB> shells experience an epitaxially segmented orientation growth. Also, the (101) crystal planes of TiO<SUB>2</SUB> crystals grow preferentially on the surface of gold nanocrystals stabilizing the heterointerfaces, then faster [001] growth results in the “budding” process occurs, producing growth sites on the initial deposition TiO<SUB>2</SUB> layers, where the TiO<SUB>2</SUB> crystals grow up into truncated wedge-shaped morphologies. It is also found that morphological evolution of TiO<SUB>2</SUB> shells is dependent on the produced F<SUP>−</SUP> ion concentration from hydrolyzed TiF<SUB>4</SUB> precursors. The produced F<SUP>−</SUP> ions not only facilitate the formation of well-defined wedge-like TiO<SUB>2</SUB> shells, but also contribute to the externally exposed truncated crystal {004} facets. As the representative photocatalyst, the catalytic activities of the resultant core−shell Au@TiO<SUB>2</SUB> nanoparticles were investigated by photoinitiated oxidation degradation of gaseous acetaldehyde. It has been indicated that the nanostructured core−shell Au@TiO<SUB>2</SUB> photocatalyst represents high photocatalytic activity when exposed to UV or visible light irradiation. The high phototocatalytic performance is also largely attributed to the preferentially grown TiO<SUB>2</SUB> shell structures and metal (Au)-TiO<SUB>2</SUB> heterointerfaces.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/langd5/2009/langd5.2009.25.issue-11/la900035a/production/images/medium/la-2009-00035a_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/la900035a'>ACS Electronic Supporting Info</A></P>