Integration for Heterogeneous Manufacturing Information Systems Based on Semantic Entity

Song Yu-chuan,Xiong Jun,LI Xian-wang,Chen Xue-hai3 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.9

To support semantic integration for heterogeneous information systems in manufacturing enterprises, based on semantic gateway, the concept of semantic entity is defined, and the semantic entity-based integration method is studied. Semantic entity sets up conceptual structure by aggregating semantic concept from semantic gateway, and builds dynamic conceptual structure through adding or removing its general concept. By means of key business data, semantic entity identifies business object and obtains business data from its application system, and then instantiates concept structure. Based on instantiation, semantic service can obtain parameters data from business object instant, and satisfies the interface contract. Semantic entity captures data changes of business object, and then establishes status-based integration rules, defines algorithm logic of rules. By instantiating concept structure aimed to specific application system and invoking semantic service, the integration activities among information systems come true. This work has been verified by prototype system, and attained expected integration effect.

Characterization of proteolysis in muscle tissues of sea cucumber Stichopus japonicus

Chen-Chen Zhao,Yang Yang,Hai-tao Wu,Zhi-Mo Zhu,Yue Tang,Cui-Ping Yu,Na Sun,Qiang Lv,Jia-Run Han,Ao-Ting Li,Jia-Nan Yan,Yue Cha 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6

The proteolysis in muscle tissues of sea cucumber Stichopus japonicus (sjMTs) was characterized. The proteins from sjMTs were primarily myosin heavy chains (MHCs), paramyosin (Pm), and actin (Ac) having a molecular mass of approximately 200, 98, and 42 kDa, respectively. Based on SDS-PAGE analysis and quantification of trichloroacetic acid (TCA)-soluble peptides released, degradation of muscle proteins from sjMTs was favorable at pH 5 and 50°C. Proteolysis of MHCs was mostly inhibited by cysteine protease inhibitors, including trans-epoxysuccinyl-L-leucyl-amido (4- guanidino) butane (E-64) and antipain (AP). E-64 and AP completely inhibited the degradation of Pm and Ac, while iodoacetic acid showed a partially inhibitory effect. These results indicated that the proteolysis of sjMTs was mainly attributed to cysteine proteases. Avoidance of setting the tissues at 40–50oC and slightly acidic condition and inhibition of cysteine proteases are helpful for decreasing sea cucumber autolysis.

Design and Analysis of an Active Balancing Mechanism for a Vertical Axis Washing Machine

Hai-Wei Chen,Xun-Ting Yuan,Zhen Sun,Qiu-Qi Mou,Ming Xiong,Wei-Hua Wang 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.23 No.7

In recent years, active balancing of washing machines has drawn much attention from manufactures. This paper proposes a novel active balancing mechanism for vibration suppression of a vertical axis washing machine which employs water for counteracting the unbalanced laundry. At first, a novel structure with two balancing planes sharing only one set of nozzles for injecting water is designed and the balancing capability of the structure is analyzed. Then, a dynamic model of the active balancing system is constructed. Vibration characteristics of the system including changes of the vibration amplitude and phase angle under different influences are studied. After that, a strategy for identifying the imbalance is proposed and an active control method is designed. At last, a washing machine armed with the active mechanism is manufactured. Both a fixed eccentric block and a pile of randomly distributed laundry are used to test the balancing effect. The results show that lateral vibrations of the washer can be greatly reduced by the active mechanism. Due to the simplicity of the structure and the satisfactory balancing effect, the mechanism proposed has a big potential to be employed in future washing machines.

Multiple Impairments in Male Reproduction 1 (mimr1), a Novel Male-Sterile Mutant of Arabidopsis thaliana, Shows Several Defects in Male Reproductive Development

Hai-Yan Chen,Yue-Feng Guan,Xue-Yong Huang,Yu-Ting Wu,Fen-Fei Wang,Ju-Fang Gao,Que Zhou,Zhong-Nan Yang,Jia-Yao Liu,Hong-Xia Zhang 한국식물학회 2012 Journal of Plant Biology Vol.55 No.3

We have characterized a new male-sterile mutant in Arabidopsis that exhibits conditional sterility but has restored fertility when drought-stressed. This mutant,multiple impairments in male reproduction 1 (mimr1),shows pleiotropic defects in both vegetative and reproductive development. Examination with dissecting and scanning electron microscopes revealed that its pollen grains are not effectively released from the anther locule after dehiscence, and anther differentiation is defective. Growth of the style and stamen filaments are also abnormal. Histological analysis demonstrated that these phenomena are due not only to a noticeably reduced extension of the stamen but also greater elongation of the pistil. Genetic analysis indicated that mimr1 is a single locus recessive nuclear mutant. The mutation can be mapped to a locus strongly linked to a 1200-kb region on Chromosome 3. Meta-analysis of expression patterning presented several candidate genes in that region. No mutants with similar phenotypes have previously been reported, suggesting that mimr1 is a novel male-sterile locus. Characterization of MIMR1 will provide further insights into the molecular basis for the development of plant reproductive organs.

Investigation of the SHM-oriented model and dynamic characteristics of a super-tall building

Hai-Bei Xiong,Ji-Xing Cao,Feng-Liang Zhang,Xiang Ou,Chen-Jie Chen 국제구조공학회 2019 Smart Structures and Systems, An International Jou Vol.23 No.3

Shanghai Tower is a 632-meter super high-rise building located in an area with wind and active earthquake. A sophisticated structural health monitoring (SHM) system consisting of more than 400 sensors has been built to carry out a long-term monitoring for its operational safety. In this paper, a reduced-order model including 31 elements was generated from a full model of this super tall building. An iterative regularized matrix method was proposed to tune the system parameters, making the dynamic characteristic of the reduced-order model be consistent with those in the full model. The updating reduced-order model can be regarded as a benchmark model for further analysis. A long-term monitoring for structural dynamic characteristics of Shanghai Tower under different construction stages was also investigated. The identified results, including natural frequency and damping ratio, were discussed. Based on the data collected from the SHM system, the dynamic characteristics of the whole structure was investigated. Compared with the result of the finite element model, a good agreement can be observed. The result provides a valuable reference for examining the evolution of future dynamic characteristics of this super tall building.

Expression Analysis of miRNAs in Porcine Fetal Skeletal Muscle on Days 65 and 90 of Gestation

Chen, Jian-hai,Wei, Wen-Juan,Xiao, Xiao,Zhu, Meng-Jin,Fan, Bin,Zhao, Shu-Hong Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.7

MiRNAs (microRNAs) are a class of small non-coding RNA molecules of ~21 nucleotides that down- regulate the expression of target genes at post-transcriptional level. In this study, we first accomplished a preliminary scan of miRNA expression using 65 and 90 day fetal pig skeletal muscle samples by microarray hybridization, and 34 miRNAs showed strong positive signals. Five of these miRNAs were selected for further investigation by real-time RT-PCR. The statistical analyses indicated that three miRNAs exhibited significant differential expression (p<0.05) during porcine muscle development from 65 to 90 days of gestation, e.g., miR-24 and miR-424 were down-regulated while miR-133a was up-regulated. Multi-tissue RT-PCR was performed to detect the expression patterns of the five miRNA precursors. The results showed that most of these precursor miRNAs were ubiquitously expressed in different porcine tissues.

Water and carbohydrate levels at different developmental stages and dynamics in hibernating pupae of Pieris melete (Lepidoptera: Pieridae)

Hai-Jun XIAO,JunHui CHEN,Chao CHEN,Fang-Sen XUE 한국곤충학회 2015 Entomological Research Vol.45 No.5

For insight into the physiological indicators of diapause in Pieris melete, water and carbohydrate (glycogen and trehalose) levels were measured under both natural and laboratory conditions. The highest water content (3.71–3.79 mg/mg dry weight) was found in larvae and developing pupae, which was substantially higher than in diapausing pupae (2.59 mg/mg dry weight). Water content was almost stable during diapause, except for individuals approaching diapause termination (3.43–3.58 mg/mg dry weight). The total carbohydrate level was significantly higher in pre‐pupae (47.41 μg/mg) compared to larvae (22.80 μg/mg) and developing pupae (21.48 μg/mg). The highest level of trehalose was detected in winter diapausing pupae, and no trehalose was found in larvae or developing pupae. Levels of glycogen were highest in pre‐pupae and lowest in diapausing pupae. Levels of total carbohydrate decreased as diapause proceeded, and no significant changes were found in trehalose levels for diapausing pupae under natural conditions or treated for 60–90 days at 5°C. Pupae treated at 20°C for 60–90 days had significantly lower levels of trehalose than those treated for 30 days. Glycogen content was relatively stable at 5°C, but increased after treatment under natural conditions and 20°C for more than 60 days. These results suggest that the dynamics of water and carbohydrate levels are potential physiological diapause indicators, which show metabolic differences between trehalose and glycogen during diapause development.

Transcriptome-wide identification of miRNA targets and a TAS3- homologous gene in Populus by degradome sequencing

Hai Bao,Min Chen,Hui Chen,Liang Du,Yanwei Wang 한국유전학회 2019 Genes & Genomics Vol.41 No.7

Background Degradome sequencing has been applied to identify miRNA-directed mRNA cleavage and understand the biological function of miRNAs and their target genes in plants defense to stress. miRNAs involved in the response to cold stress have been identified in Populus, however, there are few reports about the validated targets of miRNAs in Populus under cold stress. Objectives The primary objective of this investigation was to globally identify and validate the targets of the miRNAs and regulatory components in Populus under cold stress. Methods Populus plantlets grown in vitro were treated with cold (4 °C for 8 h) and total RNA was extracted using Trizol reagent. Approximately 200 μg total RNA was used for the construction of the degradome library, and degradome sequencing was conducted on an Illumina HiSeq 2000. The sequences were mapped to Populus genome using SOAP 2.0 and then were collected for degradome analysis. Additionally, trans-acting siRNA sequences from transacting siRNA gene 3 sequences and mature miRNAs cleaved from precursor miRNAs of Populus were analyzed. 5′ RNA ligase-mediated-RACE (5′-RACE) were further conducted. Results 80 genes were experimentally determined to be the target of 51 unique miRNAs, including three down-regulated miRNAs (pto-miR156k, pto-miR169i-m, and pto-miR394a-5p/b-5p) and two up-regulated miRNAs (pto-miR167a-d and pto-miR167f/g). The specificity and diversity of cleavage sites of miRNA targets were validated through 5′-RACE experiment and the results were similar with that of degradome sequencing, further supporting the empirical cleavage of miRNAs on targets in vivo in Populus. Interestingly, the TAS-homologous gene pto-TAS3 (EF146176.1) was identified and 11 potential ta-siRNAs [D1(+)–D11(+)] and their possible biogenesis sites within the pto-TAS3 transcript sequence were predicted in Populus. In addition, the biosynthesis of miRNA from precursor miRNA (pre-miRNA) was also validated through the detection of a total of 17 pre-miRNAs. Conclusion Our investigation expands the application of degradome sequencing for evaluating miRNA regulatory elements and evidence of the miRNA synthesis process, and provides empirical evidence of bona fide cleavage of target genes by miRNAs in Populus, which might be used for the research of miRNA-mediated regulation mechanism and molecular improvement of resistance to cold stress.

method for vibration isolation of a vertical axis automatic washing machine with a hydraulic balancer

Hai-Wei Chen,Wei-Xi Ji,Qiu-Ju Zhang,Yi Cao,Sheng-Yao Fan 대한기계학회 2012 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.26 No.2

This paper discusses a method for vibration isolation of a vertical axis automatic washing machine with a hydraulic balancer. First, a way to isolate vibration through a small amplitude of the suspension rod’s axial force is proposed, and a base circle of a cone along which the lower joint of a suspension rod moves is discussed. Based on the circle, a geometric constraint involving the slant angle of the suspension rod, the deflection angle of the washing/spinning assembly, the suspension radius of the tub and the eccentricity of the system at the steady state is derived. Considering that the trace along which the suspension rod moves is also affected by the dynamics of the system, a governing equation satisfying the equilibrium conditions of the centrifugal forces and torques is obtained. Combing the geometric constraint and governing equation together achieves a general governing equation for vibration isolation of the system. Finally, the general governing equation is proven by simulations, and the relations between the optimal installing height and several parameters are discussed.

IQGAP1 is overexpressed in hepatocellular carcinoma and promotes cell proliferation by Akt activation

Chen, Feng,Zhu, Hai-Hong,Zhou, Lin-Fu,Wu, Shan-Shan,Wang, Jing,Chen, Zhi Korean Society for Biochemistry and Molecular Bion 2010 Experimental and molecular medicine Vol.42 No.7

The scaffold protein IQGAP1 shows elevated levels in several cancer types, but its expression in hepatocellular carcinoma is unknown. We found that 58% of human hepatocellular carcinoma tissue samples had increased IQGAP1 expression compared to adjacent normal tissue. Overexpressing IQGAP1 raised the in vivo tumorigenicity of hepatocellular carcinoma cells, and forced overexpression of IQGAP1 in vitro stimulated cell proliferation. Cell growth was reduced by knockdown or mutation of IQGAP1, or by treatment of cells with a phosphotidylinositol 3-kinase inhibitor. To determine the mechanism by which IQGAP1 overexpression affected hepatocellular carcinoma cells, we confirmed its interaction in these cells with mammalian target of rapamycin (mTOR), a serine/threonine kinase that integrates signals about nutrient and energy status with downstream effectors that influence cell division. In addition, we discovered a new interaction involving IQGAP1, mTOR and Akt, which is a downstream target of mTOR. Akt phosphorylation on Ser-473, which is catalyzed by mTOR and required for Akt activation, increased with increasing amounts of IQGAP1, and decreased with IQGAP1 mutation. We hypothesize that IQGAP1 is a scaffold that facilitates mTOR and Akt interaction.