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Anticancer and Cytotoxic Effect of Verotoxin 1 on Colon Cancer Cell Line
Hadid Mustafa Attiyah,Al-Halbosiy Mohammad M. F.,Al-Shaibani Abdulwahid B. 한국미생물·생명공학회 2022 한국미생물·생명공학회지 Vol.50 No.3
Verotoxin-1 (VT-1) or Shiga-like toxin 1 (Stx-1) is produced by enterohemorrhagic Escherichia coli (EHEC) and is an AB5 holotoxin with a strong inhibitor of protein synthesis. VT-1 is a type 2 ribosomeinactivating protein (RIP) that has been shown to have cytotoxic and anticancer potential by inducing necrosis, apoptosis, and cell cycle arrest, making it a promising antitumor candidate. Here, we tested the cytotoxicity of VT-1 on CaCo2 and NCM425 cell lines and the results showed that VT-1 was more potent on CaCo2. Morphological changes were also evaluated on the cellular level and the results showed that VT-1 caused a decrease in viable cell count, altered cell membrane permeability, and an increase in total nuclear intensity. On the other hand, VT-1 displayed a lesser impact on mitochondrial membrane potential (MMP) and cytochrome c release. On the expression of caspases 3 and 9, VT-1 exhibited an insignificant effect on both which alongside the mitochondrial membrane potential (MMP) and cytochrome c results, might indicate that CaCo2 suffered from the necrosis process as a mechanism of cell death after exposure to VT-1.
Free vibration analysis of cantilever cylindrical tanks
Hadid, H.A.,Hasson, D.A. Techno-Press 1995 Structural Engineering and Mechanics, An Int'l Jou Vol.3 No.1
General free vibration characteristics of cantilevered circular cylindrical tanks are analyzed using the integral equations technique with the cubic spline functions. For computations, the partial differential equations for thin shallow shells as given by Flugge's have been employed after the addition of the inertia forces. The application of the method is illustrated with a numerical examples of tanks which are free at the top edge and fixed at the bottom. The results obtained by this method have been compared with the available results and a good agreement was found.