Development of a modified straw method for vitrification of in vitro-produced bovine blastocysts and various genes expression in between the methods

Ha, A.N.,Lee, S.R.,Jeon, J.S.,Park, H.S.,Lee, S.H.,Jin, J.I.,Sessions, B.R.,Wang, Z.,White, K.L.,Kong, I.K. Academic Press 2014 Cryobiology Vol.68 No.1

This study evaluated a modified plastic straw loading method for vitrification of in vitro-produced bovine blastocysts. A modified straw was used with a depressed area on its inner surface to which embryos attach. In vitro-produced blastocysts were randomly assigned into three groups: (i) blastocysts attached to the inner surface of a plastic straw (aV), (ii) blastocysts attached to the inner surface of a modified plastic straw (maV), and (iii) non-vitrified blastocysts (control). The recovery rates were not significantly different between aV and maV groups (95.8% vs. 94.3%). The post-thaw survival rate did not significantly differ between aV and maV groups (86.4% vs. 88.2%). The total cell numbers of blastocyst was higher in control than in aV and maV groups (142+/-21.8 vs. 117+/-29.7 and 120+/-25.2; P<0.05), but not significantly differ between aV and maV groups. The mRNA levels of pro-apoptosis related genes Bax and Caspase-3 were higher in aV and maV than in control (P<0.05). By contrast, the mRNA levels of anti-apoptotic genes Bcl-2 and Mcl-1 and of antioxidant-related genes MnSOD and Prdx5 were lower in aV and maV than in control (P<0.05). Confocal microscopy analysis of Golgi apparatus and mitochondria showed that the fluorescence intensity of Golgi apparatus and mitochondria was higher in control than in aV and maV groups. In conclusion, both aV and maV methods can be used to successfully vitrify IVP blastocysts, with maV method to be preferable because of its easiness in embryo loading.

독일학교에서의 환경교육에 관한 교육과정 및 교과서

Haan, Gerhard de 한국환경교육학회 1991 한국환경교육학회 학술대회 자료집 Vol.- No.1

Comparison of Predicted Acoustics with the Measured Acoustic Properties of a Multi-Purpose Hall

Haan, Chan-Hoon The Acoustical Society of Korea 2006 韓國音響學會誌 Vol.25 No.e3

The present study presents the design procedures and the acoustic properties of the main hall of Ansan Cultural Arts Center in Korea which has opened in 2004. The acoustic design values are compared with the measured acoustic properties of the completed multi-purpose hall. Acoustic design criteria were suggested in the design stage through the 3-dimentional computer simulations. The acoustic parameters including SPL, RT, C80, $D_{50}$M, RASTI were measured in the hall after completed. Acoustic measurements were carried out in the 40 measurement points using MLS sound source signal in 4 different sound source points. The results show the even distribution of sound levels within the 2.0dB of difference among all seats. The reverberation time of 1.66sec was measured which is similar to the objective value of 1.65 sec in empty states. It was also found that average C80 values lie in the objective extents of C80 from -1 to 3dB and average D50 value of 54 was measured. Thus, it is concluded that the hall can be used as a multi-purpose hall with a suitable acoustic conditions.

Isolation of subgroup J avian leukosis virus in Korea

Haan-Woo Sung,Jae-Hong Kim,Sanjay Reddy,Aly Fadly 대한수의학회 2002 Journal of Veterinary Science Vol.3 No.2

Two subgroup J avian leukosis viurses (ALVs) wereisolated from broiler breeder flocks,in which myeloidleukosis had occurred.The isolates could be classifiedas subgroup JALV by the positive reaction in polymerasechain reaction (PCR) with primers specific for subgroupJ ALV. Two isolates replicated in chicken embryofibroblast (CEF) cells from the alv6 chicken line inwhich cells are resistant to subgroup A and E ALVs.In in vitro serum neutralization tests with othersubgroup ALVs including ADOL-Hc1,the prototype ofsubgroup J ALVs isolated in the United States ofAmerica, two isolates were partially neutralized byantibody to ADOL-Hc1, indicating that Korean isolatesand ADOL-Hc1 may be antigenically related, but notidentical.When the PCR was done with a primer pairdesigned to amplify genes of E element and longterminal repeat of proviral DNA, the PCR productsize of one isolate (KOAL-PET) was smaller than thatof ADOL-Hc1, suggesting that some sequences inthese regions are deleted.

THE BIMA PROJECT: O-C DIAGRAMS OF ECLIPSING BINARY SYSTEMS

HAANS, G.K.,RAMADHAN, D.G.,AKHYAR, S.,AZALIAH, R.,SUHERLI, J.,IRAWATI, P.,SAROTSAKULCHAI, T.,ARIFIN, Z.M.,RICHICHI, A.,MALASAN, H.L.,SOONTHORNTHUM, B. The Korean Astronomical Society 2015 天文學論叢 Vol.30 No.2

The Eclipsing Binaries Minima (BIMA) Monitoring Project is a CCD-based photometric observational program initiated by Bosscha Observatory - Lembang, Indonesia in June 2012. Since December 2012 the National Astronomical Research Institute of Thailand (NARIT) has joined the BIMA Project as the main partner. This project aims to build an open-database of eclipsing binary minima and to establish the orbital period of each system and its variations. The project is conducted on the basis of multisite monitoring observations of eclipsing binaries with magnitudes less than 19 mag. Differential photometry methods have been applied throughout the observations. Data reduction was performed using IRAF. The observations were carried out in BVRI bands using three different small telescopes situated in Indonesia, Thailand, and Chile. Computer programs have been developed for calculating the time of minima. To date, more than 140 eclipsing binaries have been observed. From them 71 minima have been determined. We present and discuss the O-C diagrams for some eclipsing binary systems.

Development of an active gust generation mechanism on a wind tunnel for wind engineering and industrial aerodynamics applications

Haan, Fred L. Jr.,Sarkar, Partha P.,Spencer-Berger, Nicholas J. Techno-Press 2006 Wind and Structures, An International Journal (WAS Vol.9 No.5

A combination Aerodynamic/Atmospheric Boundary Layer (AABL) Wind and Gust Tunnel with a unique active gust generation capability has been developed for wind engineering and industrial aerodynamics applications. This facility is a cornerstone component of the Wind Simulation and Testing (WiST) Laboratory of the Department of Aerospace Engineering at Iowa State University (ISU). The AABL Wind and Gust tunnel is primarily a closed-circuit tunnel that can be also operated in open-return mode. It is designed to accommodate two test sections ($2.44m{\times}1.83m$ and $2.44m{\times}2.21m$) with a maximum wind speed capability of 53 m/s. The gust generator is capable of producing non-stationary gust magnitudes around 27% of the mean flow speed. This paper describes the motivation for developing this gust generator and the work related to its design and testing.

Calcium Current in the Unfertilized Egg of the Hamster

Haan, Jae-Hee,Cho, Soo-Wan,Yang, Young-Sun,Park, Young-Geun,Park, Hong-Gi,Chang, Gyeong-Jae,Kim, Yang-Mi,Park, Choon-Ok,Hong, Seong-Geun The Korean Physiological Society 1994 대한생리학회지 Vol.28 No.2

The presence of a calcium current $(i_{Ca^{2+}})$ passed via a specific channel was examined in the unfertilized hamster egg using the whole-cell voltage clamp technique. Pure inward current was isolated using a $Ca^{2+}-rich$ pipette solution containing 10 mM TEA. This current was independent of external $Na^+$ and was highly sensitive to the $Ca^{2+}$ concentration in the bathing solution, indicating that the inward current is carried by $Ca^{2+}$. The maximal amplitude was $-4.12{\pm}0.58nA\;(n=12)$ with 10mM $Ca^{2+}$ at -3OmV from a holding potential of -8OmV. This current reached its maximum within 20ms beyond -3OmV and decayed rapidly with an inactivation time constant $({\tau})$ of 15ms. Activation and inactivation of this $i_{Ca^{2+}}$ was steeply dependent on the membrane potential. The $i_{Ca^{2+}}$ began to activate at the lower voltage of -55 mV and reached its peak at -35 mV, being completely inactivated at potentials more positive than -40 mV. These result suggest that $i_{Ca^{2+}}$ in hamster eggs passes through channels with electrical properties similar to low voltage-activated T-type channels. Other results from the present study support this suggestion; First, the inhibitory effect of $Ni^{2+}\;(IC_{50}=13.7\;{\mu}M)$ was more potent than $Cd^{2+}\;(IC_{50}=123\;{\mu}M)$. Second, $Ba^{2+}$ conductance was equal to or below that of $Ca^{2+}$. Third, $i_{Ca^{2+}}$ in hamster eggs was relatively insensitive to nifedipine $(IC_{50}=96.6\;{\mu}M)$, known to be a specific t-type blocker. The physiological role of $i_{Ca^{2+}}$ in the unfertilized hamster eggs remains unclear. Analysis from steady-state inactivation activation curves reveals that only a small amount of this current will pass in the voltage range $(-70{\sim}-30\;mV)$ which partially overlaps with the resting membrane potential. This current has the property that it can be easily activated by a weak depolarization, thus it may trigger a certain kind of a intracellular event following fertilization which may cause oscillations in the membrane potential.

Regulatory Action of $\beta-adrenergic$ Agonist and 8-bromocyclic AMP on Calcium Currents in the Unfertilized Mouse Eggs

Haan, Jae-Hee,Cheong, Seung-Jin,Kim, Yang-Mi,Park, Choon-Ok,Hong, Seong-Geun The Korean Physiological Society 1993 대한생리학회지 Vol.27 No.2

There are many report suggesting that influx and intracellular calcium concentration $([Ca^{2+}]_i)$ are related to cell signalling in various cells. However, it has not been reported that calcium channel activation is affected by the substances involved in signal transduction pathways in the mouse eggs. In this study, the effects of isoprenaline (ISP) and cyclic AMP on calcium influx through calcium channels were investigated to show their relationship with the signal transduction process in unfertilized mouse eggs. Using whole cell voltage clamp techniques, calcium currents, elicited by the depolarizing pulses of 300 ms duration (from -50 mV to 50 mV in 10 mV increments) from a holding potential of -80 mV, were recorded. The current-voltage (I-V) relation of calcium currents was shown to be bell-shaped; the current began to activate at -50 mV and reached its maximum $(-1.33{\pm}0.16\;nA:\;mean{\pm}S.E.,\;n=7)$ at -10 mV, then decayed at around 50 mV. Calcium currents were fully activated within $7\;ms{\sim}20\;ms$ and completely inactivated 200 ms after onset of the step pulse. ISP within the concentration ranges of $10^{-8}\;M{\sim}10^{-4}\;M$ dose-dependently increased the amplitude calcium current. The permeable cyclic AMP analogue,8-bromocyclic AMP, also increased its maximal amplitude by 46ft at $10^{-5}\;M$, while protein kinase inhibitor (PKI), which is known to inhibit 0.02 phosphorylating units of cyclic AMP-dependent protein kinase (PKA) per microgram decreased calcium currents. Currents recorded in the presence of PKI were resistant to increase by the application of $10^{-5}\;M$. Also, PKI inhibited the calcium current increase elicited by ISP treatment. These results suggest that $\beta-adrenergic$ regulation of the calcium channel is mediated by the cAMP-dependent protein kinase. This signal transduction pathway might play a role in regulating $[Ca^{2+}]_i$, level due to the increase of calcium influx in mouse eggs.

Regulatory Action of ß-adrenergic Agonist and 8-bromocyclic AMP on Calcium Currents in the Unfertilized Mouse Eggs

Haan. Jae-Hee,Cheong. Seung-Jin,Kim. Yang-Mi,Park. Choon-Ok,Hong. Seong-Geun 대한생리학회 1993 대한생리학회지 Vol.27 No.2

There are many report suggesting that influx and intracellular calcium concentration ([Ca²⁺]_i) are related to cell signalling in various cells. However, it has not been reported that calcium channel activation is affected by the substances involved in signal transduction pathways in the mouse eggs. In this study, the effects of isoprenaline (ISP) and cyclic AMP on calcium influx through calcium channels were investigated to show their relationship with the signal transduction process in unfertilized mouse eggs. Using whole cell voltage clamp techniques, calcium currents, elicited by the depolarizing pulses of 300 ms duration (from -50 mV to 50 mV in 10 mV increments) from a holding potential of -80 mV, were recorded. The current-voltage (I-V) relation of calcium currents was shown to be bell-shaped; the current began to activate at -50 mV and reached its maximum (-1.33±0.16 nA: mean±S.E., n=7) at -10 mV, then decayed at around 50 mV. Calcium currents were fully activated within 7 ms ~ 20 ms and completely inactivated 200 ms after onset of the step pulse. ISP within the concentration ranges of 10^-8 M ~ 10^-4 M dose-dependently increased the amplitude calcium current. The permeable cyclic AMP analogue,8-bromocyclic AMP, also increased its maximal amplitude by 46ft at 10^-5 M, while protein kinase inhibitor (PKI), which is known to inhibit 0.02 phosphorylating units of cyclic AMP-dependent protein kinase (PKA) per microgram decreased calcium currents. Currents recorded in the presence of PKI were resistant to increase by the application of 10^-5 M. Also, PKI inhibited the calcium current increase elicited by ISP treatment. These results suggest that β-adrenergic regulation of the calcium channel is mediated by the cAMP-dependent protein kinase. This signal transduction pathway might play a role in regulating [Ca²⁺]_i, level due to the increase of calcium influx in mouse eggs.

[발언과 논평] 문화건축물의 외형적, 내재적 가치

한찬훈(Haan, Chan Hoon) 대한건축학회 2016 建築 Vol.60 No.10